흰쥐 난소에서 황체내 큰포식세포에 관한 형태학적 연구 : 면역조직화학적 및 투과전자현미경적 연구

김원식,한승로,조근자,김수일,박창식 충남대학교 형질전환복제돼지연구센터 2007 논문집 Vol. No.10

황체는 임신의 수립과 유지에 필수적인 일시적인 내분비샘으로, 주기능을 발정주기의 생리적 기간을 결정하고 성공적인 임신을 달성하는데 결정적인 프로게스테론의 생산이다. 황체의 기능은 뇌하수체 gonadotropin 뿐만 아니라 황체내 큰포식세포에서 분비되는 TNF-α를 포함한 몇가지 cytokines에 의해 조절되는 것으로 알려져 있다. 본 연구에서는 흰쥐 (Sprague-Dawley계통)에서 생식주기별 황체내 큰포식세포의 변화를 면역조직화학적 및 투과전자현미경적으로 관찰 비교하였다. 본 연구에서 흰쥐 황체에서 큰포식세포는 배란기,임신기 및 출산후기 등 모든 시기에 나타나고,그 수와 면역반응성은 배란기에 가장 컸고 출산후기,임신기 순으로 나타났다. 아울러 투과전자현미경 관찰에서는 황체내 큰포식세포를 세포체가 가늘고 돌기가 긴 비포식성 큰포식세포와 포식성 공포와 지방소체들을 많이 함유한 포식성 콘포식세포로 분류할 수 있었다. 본 연구를 통해 황체내 콘포식세포는 발정주기에 따라 그 수나 면역반응성이 다르며,황체퇴화가 활발히 진행되는 배란기와 출산후기에 면역반응성이 증가하고 포식성 큰식세포들이 나타난 것은 큰포식세포에서 TNF-α 와 같은 cytokines의 분비를 활발히 일으키고 그들이 황체세포들의 세포자멸사를 유발,촉진하고 또한 세포자멸 사소체들을 포식,제거하는 방법으로 황체퇴화를 유도하여 정상적인 발정주기를 유지하기 위한 기전으로 생각 되고,임신기에 큰 포식세포의 수와 면역반응성이 작아지고 비포식성 큰포식세포가 주를 이루는 것은 이들이 TNF-α 동의 cytokines 분비를 통해 그들이 황체자극효과를 나타내 황체의 기능을 유지,촉진하는데만 관여하고,한편으로는 황체에서 생산되는 프로게스테론에 의한 큰포식세포 억제작용 때문인 것으로 생각된다. Macrophages in the corpus luteum have many important roles during the periods of functional development and luteal regression. Not only phagocyte the apoptotic luteal cells, but also they secrete many cytokines and exert their effects via autocrine/paracrine actions. In this study, we investigated the changes of number and immunoreactivity of macrophages at various developmental periods of the corpus luteum in the rat ovary. The rats (Sprague- Dawley strain, female) at age of 8 weeks (ovulatory period), GD 6 (pregnant period), and postpartum 5 days (postpartum period) were sacrificed under ether anesthesia and obtained both ovaries, one used for macrophages immunohistochemistry and the other used for TEM. The results were as follows; I. In the corpora lutea of the rat, macrophages were observed all the developmental periods including ovulatory, pregnant and postpartum periods. 2. In the corpora lutea of the rat, number of macrophages was highest in the ovulatory period, and decreased at postpartum period and pregnant period in order. The immunoreactivity of macrophages was high at ovulatory period, moderate at postpartum period, and low at pregnant period. 3. In TEM observations, two types of macro phages were observed: One type was non-phagocytic macrophage and the other type was phagocytic macrophage. Phagocytic macrophages were observed in the corpora lutea at ovulatory and postpartum periods and contained apoptotic bodies, phagocytic vacuoles and many lipid droplets. Non-phagocytic macrophages were observed in the corpora lutea at pregnancy period and showed slender cell body with long cytoplasmic processes and contained no apoptotic bodies. In the rat, the number and the degree of immunoreactivity of macrophages in the corpus luteum varied with the changes of functional state of the corpus luteum. It was suggested that the main function of the macrophages at the ovulatory and postpartum periods was elimination of apoptotic luteal cells and that at pregnancy period was autocrine/paracrine action. Ultrastructurally, two types, phagocytic and nonphagocytic types, of macrophages confirmed. These results will provide valuable informations on the study of the role macrophages during development and regression of corpus luteum.

방사선 조사에 의한 흰주 난포의 퇴축과 난포세포의 방사선 감수성

김수일,한승로,조근자,허대영,이영호,조문준,김무강,김원식 충남대학교 의과대학 의학연구소 2002 충남의대잡지 Vol.29 No.1

The aim of this study were to investigate radiation-induced atresia and radiation susceptibility in the rat ovary morphologically. Female rats (Sprague Dawley strain) of 4 weeks old were irradiated with dose of 4 Gy and 8 Gy, and sacrificed at hour 6, 12, and 24 after radiation. The H & E stain, the TUNEL method (ApopTag kit) and transmission electron microscopy (TEM) were used. In light microscopic observation, the number of atresia of ovarian follicles were increased significantly at 6h after irradiation (p<0.01). There was no significant difference between 4 Gy and 8 Gy irradiated groups. In TEM observation, granulosa cells in radio-sensitive follicles were characterized by several structural features including condensation of nuclear chromatin granules, nuclear fragmentation and apoptotic body formation. An apoptotic cell is observed to have been phagocytosed by a normal granulosa cell. Granulosa cells in radio-resistant follicles were characterized by several structural features including nuclear indentation, partial condensation of chromatin granules, mitochondrial swelling, dilatation of RER cisterns, accumulation of dense irregular masses, accumulation of lipid droplets, and increased lysosomal bodies. Number of gap junctions between granulosa cells were decreased, and intercellular space were widen than that of control animals. These findings were prominent at 6h after irradiation and were diminished at 12h and 24h after irradiation. With these results, it was concluded that radiation-induced follicular cell apoptosis and ovarian follicular atresia in rat ovary increased considerably at 6h after irradiation. Further studies are needed to reveal the more extensive differences between radiosensitive and radioresistant follicular granulosa cells.

3D 프린팅으로 제작한 스트레인게이지를 이용한 어깨 모션 인식 시스템 개발

조근식(Geun Sik Cho),기범근(Beom-Geun Ki),권병수(Byeong Su Kwon),박용재(Yong-Jai Park) 한국생산제조학회 2023 한국생산제조학회지 Vol.32 No.2

Maintaining good health is a top priority for many people, particularly active seniors with a variety of physical activities. The shoulder is a complex joint that is particularly susceptible to injury, but it plays a crucial role in many physical activities. This study aimed to measure using a strain gauge manufactured by a 3D printer to detect shoulder motion. The sensor was fabricated using carbon nanotube (CNT) and nylon conductive filaments, and TPU filaments. The sensor in this paper was manufactured by printing three conductive lines on the main body printed with TPU. The manufactured sensor was attached to the shoulder to measure the motion of the shoulder. In this paper, we confirmed the possibility of detecting shoulder movement of sensors through experiments measuring three shoulder motions.

노후된 잔교식의 항만구조물의 신뢰성에 기초한 안전성 및 내하력평가

조효남,김성훈,노홍식,임종권,이우근 한양대학교 공학기술연구소 1995 공학기술논문집 Vol.4 No.1

This study is intended to propose a reliability based approach to safety assessment and berth-capacity rating of aged wharf structures based on measured data acquired from an extensive dynamic field load testing against berth impact of real ships. The study comprises the essential part of a project for assessing actual berth-capacity of deteriorated and damaged dolphin structures at the Onsan oil refinery wharf currently operated for the berth of oil refinery ships of 20,000 DWT.

재배년수와 토성이 도라지 생육 및 수량에 미치는 영향

조주식,윤한대,송근우,이영한,최용조,김민근 한국환경농학회 2000 한국환경농학회지 Vol.19 No.1

재배년수와 토성이 도라지 생육 및 수량에 미치는 영향을 연구한 결과는 다음과 같다. 재배년수가 2년에서 8년까지 양질사토에서 시험한 결과 재배년수와 1근중은 Y=17.24X+0.95(R²=0.948^(***)), 발병율은 Y=12.01X+5.74(R²=0.946^(***))로서 고도의 유의성이 있었다. 재배년수가 길수록 뿌리의 T-N, P₂O_5, MgO 함량은 감소되었고 장해근은 정상근에 비해 T-N, P₂O_5, K₂O 함량이 높았다. 도라지 2년생을 양질사토, 사양토, 미사질양토와 식양토에서 시험한 결과 시험후 pH는 미사질양토가 6.4였으며 다른 토성에서는 5.7∼5.8의 수치를 나타내었고 P₂O_5 및 Fe 함량을 미사질양토에서 높았다. 도라지 생육상황은 미사질양토에서 지상중 10.38g, 근경 1.34㎝, 근장 16.8㎝, 초장 57.9㎝로 양호하여 1근중이 5.46g으로 가장 높았다. This study was conducted to determine the characteristics of continuous culture and soil texture and their effect on growth and yield of platycodon. The continuous cropping year was treated from 2-years old to 8-years old. The continuous cropping year was correlated with root weight Y-17.246X+0.95(R²=0.948^(***)) and root disease incidence rate Y=12.01X-5.74(R²=0.946^(***)). The T-N, P₂O_5 and MgO content of root were yearly decresed and those of injury roots were higher than those of normal roots. The 2-years old platycodon was treated different soil texture(Loamy sand, Sandy loam, Silt loam, Clay loam). The shoot weight 10.38g, root diameter 1.34㎝, root height 16.8㎝ and root weight 5.46g at silt loam were higher than other soil texture.

Preparation of a Nickel Nanopowder by Wire Explosion in Liquid Media

Cho, Chuhyun,Ha, Yoon-Cheol,Kang, Chungil,Jin, Yun-Sik,Rim, Geun-Hie Korean Physical Society 2010 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.57 No.6

원발성 과호산구성 증후군 1예

조종근,홍대식,박희숙 순천향대학교 1988 논문집 Vol.11 No.1

The idiopathic hypereosinophilic syndrome represents a heterogenous group of disorders with the common features of prolonged eosinophilia of an undetectable cause and organ system dysfunction. The diagnostic criteria are 1) a persistant eosinophilia of 1500 eosinophils/cu mm for longer than six months or death before six months associated with signs and symtoms of hypereosinophilic disease; 2) lack of evidence for other causes of eosinophilia; 3) evidence of organ system involvement. We have experienced a case of idiopathic hypereosinophilic syndrome in a 29 years old man who complained of pain on right upper quadrant of abdomen and generalized maculopapular skin eruption. We confirmed it with biopsies of bone marrow, skin, liver and duodenum which showed diffuse eosinophilic infiltration, therefore he has been treated with prednisone and hydoxyurea with good response. And now he is treated with a small dose of prednisone.

가상점원 : 고객과의 협상을 위한 에이전트

조의성,조근식 한국지능정보시스템학회 1999 한국지능정보시스템학회 학술대회논문집 Vol.1 No.1

Lymphocytic Hypophysitis in a Patient with Testiculal Feminization Syndrome

Cho, Tack-Geun,Kwon, Jeong-Taik,Nam, Teak-Kyun,Suk, Jong-Sik The Korean Neurosurgical Society 2007 Journal of Korean neurosurgical society Vol.41 No.1

Lymphocytic hypophysitis is a rare inflammatory disease of the pituitary gland that is one of the cause of hypopituitarism. The majority of cases occur in women during the peripartum period, and it is rare in men. Testicular feminization syndrome is a genetic disorder presenting a 46XY karyotype, but a normal female phenotype. We report a case of lymphocytic hypophysitis in a 23-year-old woman with testicular feminization syndrome.

전자거래상에서의 구매자와 자동협상 수행을 위한 가상점원 시스템

조의성,조근식 한국지능정보시스템학회 1999 지능정보연구 Vol.5 No.2