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( Eun Byul Lee ),( Jung Eun Choi ),( Jung Hee Kim ),( Wonhee Hur ),( Tian Zhu Li ),( Sung Woo Kim ),( Sung Woo Hong ),( Young Ki Lee ),( Sung Min Kim ),( Joon Ho Lee ),( Seung Kew Yoon ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1
Background: Hepatitis E virus (HEV) is a hepatotropic virus and has shown the ability of self-assembly through its N-terminally truncated ORF2 (Nt-ORF2). Recently, many researchers have attempted to use the virus like particles (VLPs) as a gene delivery vehicle. In this study, we established the liver specific delivery system using HEV-LP produced in Huh7 cells by recombinant baculovirus expression system. Methods: To produce HEV-LP in mammalian cells, the recombinant baculovirus containing the CMV promoter derived Nt-ORF2 gene was generated in the insect cells, Sf21. Subsequently, the baculovirus was used to infect into the human hepatoma cell line, Huh7 cells. The expression of Nt-ORF2 in Huh7 cells was confirmed by western blot analysis. In addition, the formation of HEV-LP particle was observed by electron microscopy. Next, to determine the liver-specific entrance of HEV-LP, FITC-conjugated HEV-LP infected into the cancer cell lines derived from various organs. After infection, the fluorescence existed in infected cells was detected by confocal microscopy. Results: Nt-ORF2 was highly expressed in Huh7 cells infected with recombinant baculoviruses. Nt-ORF2 expressed in the cells showed the ability of self-assembly and release from the cells. The HEV-LPs produced from Huh7 cells was observed 25 nm in diameter. Furthermore, HEV-LP could enter into the hepatoma cell lines within 4 hr post-infection. Conclusions: These results suggest that HEV-LP was efficiently produced and infected in hepatoma cells and established HEV-LP system could be used as the valuable liver specific transporter to deliver the therapeutic agents.
박은지 ( Eun-ji Park ),장새별 ( Sae-byul Jang ),백선은 ( Seon-eun Baek ),김선경 ( Seon-kyung Kim ),유호룡 ( Ho-ryong Yoo ),유정은 ( Jeong-eun Yoo ),정인철 ( In-chul Jung ) 대한한방부인과학회 2017 大韓韓方婦人科學會誌 Vol.30 No.1
Objectives: Polycystic Ovarian Syndrome (PCOS) is characterized by ovulatory disorder, polycystic ovaries and clinical or laboratory hyperandrogenemia, also associated with an increased risk of various other long-term complications. The purpose of this study is to develop a standard instrument of pattern identifications in Korean medicine for PCOS. Methods: We retrieved the patterns and symptoms from Korean and Chinese literatures which mentioned pattern identifications of PCOS. In order to develop the instrument, we took the consultation from the advisor committee based on the collected informations from literatures. Finally the questionnaire of pattern identification for PCOS was developed. Results: 1) 5 pattern identifications and 53 symptoms and signs were selected from 20 references. 2) We obtained the mean weights which reflected the standard deviations from each symptom of the pattern by 15 experts. 3) We designed the Korean medicine Instrument on pattern identification for PCOS. It was composed of 61 questions, 44 of patient-reported format and 17 of assessor-reported format. Conclusions: Instrument of pattern identification for PCOS was developed through experts` discussion. Further study is required to identify the validity and reliability of this pattern identification instrument for PCOS.
( Hyuk Jung ),( Joo Kyoung Jung ),( Sat Byul Kim ),( Eun A Cho ),( Mi Jung Um ) 대한폐경학회 2018 대한폐경학회지 Vol.24 No.2
Objectives: To evaluate the histologic effects of tamoxifen on the endometrium using hysteroscopy in postmenopausal women with breast cancer. Methods: The study included 46 postmenopausal patients who were referred from another clinic due to thickening or bleeding of the endometrium after taking tamoxifen for breast cancer. All patients underwent transvaginal sonography (TVS) and hysteroscopic endometrial biopsy with a 5-mm, continuous-flow, operating hysteroscope. Results: The incidence of malignancy was high (20%) in cases of abnormal uterine bleeding (AUB) after taking tamoxifen. However, in the non-AUB group with thick endometrium after taking tamoxifen, the incidence of adenocarcinoma was 3.2%. Conclusions: Our findings confirm the estrogen-like effect of tamoxifen on the endometrium. Endometrial evaluation with TVS suggests further diagnostic procedures; moreover, histologic examination is necessary under hysteroscopy, especially in cases of endometrial bleeding after taking tamoxifen. (J Menopausal Med 2018;24:81-86)
조은별 ( Eun Byul Cho ),이민경 ( Min Kyung Lee ),정지윤 ( Ji Yun Jung ),박은주 ( Eun Joo Park ),김광호 ( Kwang Ho Kim ),김광중 ( Kwang Joong Kim ) 대한피부과학회 2016 大韓皮膚科學會誌 Vol.54 No.5
Although the coexistence of vitiligo and psoriasis is well known and considered not unusual, psoriasis localized exactly on vitiliginous lesions has been rarely reported. We report a 13-year-old girl with erythematous scaly plaques localized only on the depigmented vitiliginous patches after narrow-band UVB therapy. Biopsy samples were taken from the areas where the new erythematous plaques occurred. Considering the clinical findings and the biopsy results, our case corresponds to psoriasis co-localized with vitiligo. After the patient discontinued phototherapy and started oral cyclosporine, the psoriatic lesions improved. We report a rare case of psoriasis arising strictly on vitiligo lesions. (Korean J Dermatol 2016;54(5):385∼388)