Study on Chinese Consumers’ Purchase Intention on Cosmetics PPL in Korean(Hallyu) Drama

Deng Xiao-Wen,Soon-Hong Kim 한국유통과학회 2015 KODISA ICBE (International Conference on Business Vol.2015 No.-

In terms of an implication of this study, it was confirmed that hallyu celebrity-oriented imitative psychology, South Korean cosmetics brand image, PPL attitude and others have effects on purchase intention on South Korean cosmetic products and in particular, of the path coefficients, this PPL attitude variable was proved to be the most influential factor and after all, PPL strategies of South Korea’s dramas were verified as a marketing strategy that would work effectively on Chinese consumers. South Korea needs to keep creating cultures and dramas of good quality, and they also must come up with strategies to promote their creations to China.

An Effective Method for Extraction of Glutaryl-7-aminocephalosporanic Acid Acylase from Recombinant E. coli Cells

Xiao-qiang Ma,Er-zheng Su,Sen-wen Deng,Dong-zhi Wei 한국생물공학회 2015 Biotechnology and Bioprocess Engineering Vol.20 No.4

In this study, several chemical treatment techniques commonly used for protein extraction were investigated for recovering glutaryl-7-aminocephalosporanic acid acylase (GLA) from recombinant E. coli cells. The best results were obtained by the combined use of cetyltrimethylammonium bromide (CTAB) and KCl. Subsequently, various extraction conditions, such as cation salt, concentrations of CTAB and KCl, extraction temperature, extraction time, and biomass, were optimized to further enhance the release yield and specific activity of GLA. Our results showed that 110% of GLA was released after treatment with 0.5% CTAB (w/v, %) and 0.3 M KCl at 10°C for 12 h, and its specific activity in this extracting solution was approximately 1.5 times higher as compared to that obtained by sonication. This extraction method could avoid the inactivation of GLA caused by drastic mechanical methods, and also enhance its specific activity for industrial extraction.

Concept and Analysis of Stay Cables with a CFRP and Steel Composite Section

Wen Xiong,C.S. Cai,Rucheng Xiao,Lu Deng 대한토목학회 2012 KSCE Journal of Civil Engineering Vol.16 No.1

This paper describes the concept of a novel stay cable with a Carbon Fiber Reinforced Plastic (CFRP) and steel composite section. In this concept CFRP core conserves all the advantages of CFRP materials such as light weight and high strength, while steel coat provides protection for the CFRP core and ensures reliable anchorage performance. The steel coat can reduce cost and provide higher stiffness than CFRP materials in some length range. Following this concept, several configurations were proposed for the composite structure. Effects of the key design parameter of the proposed stay cables, namely the ratio of the CFRP section area to the whole section area, were examined through a parametric study using analytical solutions. By doing this, an appropriate range of the ρ value was suggested. Factors that can affect the appropriate range of the ρ value, including the horizontal projected length of stay cables,cable force, and pylon height (i.e., vertically projected length of stay cables), were also studied. Finally, the feasibility of the proposed stay cables was conceptually verified. It was shown that the proposed stay cables could be an excellent alternative to the pure CFRP or traditional steel stay cables.

A prospective randomized multicenter trial for lymphadenectomy in early- stage ovarian cancer: LOVE study

Ting Deng,Kaijiang Liu,Liang Chen,Xiao-jun Chen,Hua Wen Li,Hongyan Guo,Huijiao Zhang,Libing Xiang,Xin Feng,Xiaoyu Wang,Hextan Y. S. Ngan,Jianguo Zhao,Dongling Zou,Qing Liu,Jihong Liu 대한부인종양학회 2023 Journal of Gynecologic Oncology Vol.34 No.3

Background: The Lymphadenectomy in Ovarian Neoplasms (LION) study revealed that systemic lymphadenectomy did not bring survival benefit for advanced ovarian cancer patients with clinically normal lymph nodes and was associated with a higher incidence of operative complications. However, there is no consensus on whether lymphadenectomy has survival benefit or not in early epithelial ovarian cancer (EOC). Methods: We designed the LOVE study, a multicenter, randomized controlled, phase III trial to compare the efficacy and safety of comprehensive staging surgery with or without lymphadenectomy in stages IA-IIB EOC and fallopian tube carcinomas (FTC). The hypothesis is that the oncological outcomes provided by comprehensive staging surgery without lymphadenectomy are non-inferior to those of conventional completion staging surgery in early-stage EOC and FTC patients who have indications for post-operative adjuvant chemotherapy. Patients assigned to experimental group will undergo comprehensive staging surgery, but lymphadenectomy. Patients assigned to comparative group will undergo completion staging surgery including systematic pelvic and para-aortic lymphadenectomy. All subjects will receive 3–6 cycles of standard adjuvant chemotherapy. Major inclusion criteria are pathologic confirmed stage IA-IIB EOC or FTC, and patients have indications for adjuvant chemotherapy either confirmed by intraoperative fast frozen section or previous pathology after an incomplete staging surgery. Major exclusion criteria are non-epithelial tumors and low-grade serous carcinoma. Patients with severe rectum involvement which lead to partial rectum resection will be excluded. The sample size is 656 subjects. Primary endpoint is disease-free survival.

Association of Methylation of the RAR-β Gene with Cigarette Smoking in Non-Small Cell Lung Cancer with Southern-central Chinese Population

Li, Wen,Deng, Jing,Wang, Shuang-Shuang,Ma, Liang,Pei, Jiang,Zeng, Xiao-Xi,Tang, Jian-Xin Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.24

Pathogenesis of lung cancer is a complicated biological process including multiple genetic and epigenetic changes. Since cigarette smoking is confirmed as the most main risk factor of non-small cell lung cancer (NSCLC), the aim of this study was to determine whether tobacco exposure plays a role in gene methylation. Methylation of the RAR-${\beta}$ gene were detected using methylation-specific polymerase chain reaction in DNA from 167 newly diagnosed cases with NSCLC and corresponding 105 controls. A significant statistical association was found in the detection rate of the promoter methylation of RAR-${\beta}$ gene between NSCLC and controls ($x^2$=166.01; p<0.01), and hypermethylation of the RAR-${\beta}$ gene was significantly associated with smoking status (p=0.038, p<0.05). No relationship was found between RAR-${\beta}$ gene methylation and pathologic staging including clinical stage, cell type, gender and drinking (p>0.05), and the methylation of RAR-${\beta}$ gene rate of NSCLC was slightly higher in stages III+IV (80.0%) than in I+II (70.8%). Similar results were obtained for methylation of the RAR-${\beta}$ gene between squamous cell carcinoma (77.9%) and other cell type lung cancer (73.9%). These results showed that the frequency of methylation increased gradually with the development of clinical stage in smoking-associated lung cancer patients, and tobacco smoke may be play a potential role in RAR-${\beta}$ gene methylation in the early pathogenesis and process in lung cancer, particularly squamous cell carcinoma. Aberrant promoter methylation is considered to be a promising marker of previous carcinogen exposure and cancer risk.

Application of Computed Tomography for Differential Diagnosis of Glioma Stoke and Simple Cerebral Hemorrhage

Li, Xiao-Li,Zhou, Fa-Ming,Shangguan, Shou-Qin,Zou, Wen-Qin,Deng, Yan-Qing,Chen, Tao,Chen, Guang-Hui Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.8

Objective: To explore the value of computed tomography (CT) in the differential diagnosis of glioma stroke and simple cerebral hemorrhage. Materials and Methods: A total of 45 patients with glioma stroke and stroke as the initial symptom in our hospital from Jun., 2009 to Oct., 2013 were selected along with 50 individuals with simple cerebral hemorrhage in the same period randomly collected as a control group. The CT results in both groups were analyzed and compared. Results: In the observation group, there were 25 patients with astrocytoma (55.6%), 11 with oligodendroglioma (24.4%), 8 with ependymoma (17.2%) and 1 with glioblastoma multiforma (GBM, 2.22%). Additionally, the major CT manifestation was coexistence of hemorrhage and tumor signs. By comparison, it could be found that the proportions of patients respectively with peripheral edema and space-occupying effect in the observation group were significantly higher than in the control group (P<0.01). Conclusions: Application of CT examination combined with medical history in patients has very important clinical value in the differential diagnosis of glioma stroke and simple cerebral hemorrhage.

Staged Improvement in Awareness of Disease for Elderly Cancer Patients in Southern China

Li, Xing,Dong, Min,Wen, Jing-Yun,Wei, Li,Ma, Xiao-Kun,Xing, Yan-Fang,Deng, Yun,Chen, Zhan-Hong,Chen, Jie,Ruan, Dan-Yun,Lin, Ze-Xiao,Wang, Tian-Tian,Wu, Dong-Hao,Liu, Xu,Hu, Hai-Tao,Lin, Jia-Yu,Li, Zhu Asian Pacific Journal of Cancer Prevention 2015 Asian Pacific journal of cancer prevention Vol.16 No.15

Background: In mainland China, awareness of disease of elderly cancer patients largely relies on the patients' families. We developed a staged procedure to improve their awareness of disease. Materials and Methods: Participants were 224 elderly cancer patients from 9 leading hospitals across Southern China. A questionnaire was given to the oncologists in charge of each patient to evaluate the interaction between family and patients, patient awareness of their disease and participation in medical decision-making. After first cycles of treatment, increased information of disease was given to patients with cooperation of the family. Then patient awareness of their disease and participation in medical decision-making was documented. Results: Among the 224 cancer elderly patients, 26 (11.6%) made decisions by themselves and 125 (55.8%) delegated their rights of decision-making to their family. Subordinate family members tended to play a passive role in decision-making significantly. Patients participating more in medical decision-making tended to know more about their disease. However, in contrast to the awareness of disease, patient awareness of violation of medical recommendations was reversely associated with their participation in medical decision-making. Improvement in awareness of diagnosis, stages and prognosis was achieved in about 20% elderly cancer patients. About 5% participated more actively in medical decision-making. Conclusions: Chinese elderly cancer patient awareness of disease and participation in medical decision-making is limited and relies on their family status. The staged procedure we developed to improve patient awareness of disease proved effective.

Reconstructed Adeno-Associated Virus with the Extracellular Domain of Murine PD-1 Induces Antitumor Immunity

Elhag, Osama A.O.,Hu, Xiao-Jing,Wen-Ying, Zhang,Li, Xiong,Yuan, Yong-Ze,Deng, Ling-Feng,Liu, De-Li,Liu, Ying-Le,Hui, Geng Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.8

Background: The negative signaling provided by interactions of the co-inhibitory molecule, programmed death-1 (PD-1), and its ligands, B7-H1 (PD-L1) and B7-DC (PD-L2), is a critical mechanism contributing to tumor evasion; blockade of this pathway has been proven to enhance cytotoxic activity and mediate antitumor therapy. Here we evaluated the anti-tumor efficacy of AAV-mediated delivery of the extracellular domain of murine PD-1 (sPD-1) to a tumor site. Material and Methods: An rAAV vector was constructed in which the expression of sPD-1, a known negative regulator of TCR signals, is driven by human cytomegalovirus immediate early promoter (CMV-P), using a triple plasmid transfection system. Tumor-bearing mice were then treated with the AAV/sPD1 construct and expression of sPD-1 in tumor tissues was determined by semi quantitative RT-PCR, and tumor weights and cytotoxic activity of splenocytes were measured. Results: Analysis of tumor homogenates revealed sPD-1 mRNA to be significantly overexpressed in rAAV/sPD-1 treated mice as compared with control levels. Its use for local gene therapy at the inoculation site of H22 hepatoma cells could inhibit tumor growth, also enhancing lysis of tumor cells by lymphocytes stimulated specifically with an antigen. In addition, PD-1 was also found expressed on the surfaces of activated CD8+ T cells. Conclusion: This study confirmed that expression of the soluble extracellular domain of PD-1 molecule could reduce tumor microenvironment inhibitory effects on T cells and enhance cytotoxicity. This suggests that it might be a potential target for development of therapies to augment T-cell responses in patients with malignancies.

Deep-blue organic light-emitting diodes based on multi-tert-butyl modified naphthylene

Bin Wei,Hanfei Xu,Ning Zhao,Xicun Gao,Yiteng Ye,Yingnan Wang,Dengli Yao,Xiao-Wen Zhang,Xiujuan Liu 한국공업화학회 2021 Journal of Industrial and Engineering Chemistry Vol.102 No.-

Six tert-butyls modified naphthyl derivative (2,6-di-tert-butyl-1,5-bis-(3,5-di-tert-butyl-phenyl)-naphthalene (N(dtb)4) was synthesized and tested for host and guest materials in organic light-emittingdiodes (OLEDs). Blue OLED using N(dtb)4 as a host and an emitter DSA-PH as a guest reaches an efficiencyof 11.52 cd A 1 (maximum external quantum efficiency 7.8 % and maximum power efficiency10.33 lm W 1). In addition, for the OLED with N(dtb)4 being a guest, we find that the concentrations ofN(dtb)4 will also affect the performance of the OLED, among which ADN: 5 wt.% N(dtb)4 has higher efficiency,and the maximum current efficiency, external quantum efficiency (EQE) and power efficiency ofthe device reach 3.75 cd A 1, 3.93 % and 3.36 lm W 1, respectively. We also investigated the pure N(dtb)4-based ultraviolet OLED (UV-OLED), and achieved the maximum EQE of the device is 1.12 %. These resultsindicate that N(dtb)4, a novel synthesized fluorescent organic material, has great potential applications inOLEDs and accordingly advancing lightings and displays.

Multiple Cytotoxic Factors Involved in IL-21 Enhanced Antitumor Function of CIK Cells Signaled through STAT-3 and STAT5b Pathways

Rajbhandary, S.,Zhao, Ming-Feng,Zhao, Nan,Lu, Wen-Yi,Zhu, Hai-Bo,Xiao, Xia,Deng, Qi,Li, Yu-Ming Asian Pacific Journal of Cancer Prevention 2013 Asian Pacific journal of cancer prevention Vol.14 No.10

Background/Objectives: Maintenance of cellular function in culture is vital for transfer and development following adoptive immunotherapy. Dual properties of IL-21 in activating T cells and reducing activation induced cell death led us to explore the mechanism of action of IL-21 enhanced proliferation and cytotoxic potential of CIK cells. Method: CIK cells cultured from PBMCs of healthy subjects were stimulated with IL-21 and cellular viability and cytotoxicity to K562 cells were measured. To elucidate the mechanism of action of IL-21, mRNA expression of cytotoxic factors was assessed by RT-PCR and protein expression of significantly important cytotoxic factors and cytokine secretion were determined through flow cytometry and ELISA. Western blotting was performed to check the involvement of the JAK/STAT pathway following stimulation. Results: We found that IL-21 did not enhance in vitro proliferation of CIK cells, but did increase the number of cells expressing the CD3+/CD56+ phenotype. Cytotoxic potential was increased with corresponding increase in perforin ($0.9831{\pm}0.1265$ to $0.7592{\pm}0.1457$), granzyme B ($0.4084{\pm}0.1589$ to $0.7319{\pm}0.1639$) and FasL ($0.4015{\pm}0.2842$ to $0.7381{\pm}0.2568$). Interferon gamma and TNF-alpha were noted to increase ($25.8{\pm}6.1ng/L$ to $56.0{\pm}2.3ng/L$; and $5.64{\pm}0.61{\mu}g/L$ to $15.14{\pm}0.93{\mu}g/L$, respectively) while no significant differences were observed in the expression of granzyme A, TNF-alpha and NKG2D, and NKG2D. We further affirmed that IL-21 signals through the STAT-3 and STAT-5b signaling pathway in the CIK cell pool. Conclusion: IL-21 enhances cytotoxic potential of CIK cells through increasing expression of perforin, granzyme B, IFN-gamma and TNF-alpha. The effect is brought about by the activation of STAT-3 and STAT-5b proteins.