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Microporous organic network nanoparticles for dual chemo-photodynamic cancer therapy
Kim, Dong Wook,Kim, Da Hye,Jang, June Young,Ko, Yoon-Joo,Lee, Sang Moon,Kim, Hae Jin,Na, Kun,Son, Seung Uk The Royal Society of Chemistry 2019 Journal of Materials Chemistry B Vol.7 No.26
<P>This work shows that microporous organic network (MON) chemistry can be applied to the dual photodynamic and chemical therapy of cancer cells. Water-compatible and Zn-phthalocyanine (ZnPhT) loaded MON nanoparticles were engineered through size-controlled synthesis using poly(vinylpyrrolidone) (PVP) as a surfactant, followed by postsynthetic sulfonation of materials. The ZnPhT was successfully loaded on the sulfonated MON nanoparticles (N-SMONs) <I>via</I> coulombic interaction between anionic sulfonate and cationic ZnPhT. Because of their microporosity and high surface area, DOX was loaded efficiently on the ZnPhT/N-SMON nanoparticles. The resultant DOX/ZnPhT/N-SMON showed synergistic performance in the dual photodynamic and chemotherapy of cancer cells and tumors in <I>in vitro</I> and <I>in vivo</I> tests.</P>
Da Yoon Yu,Sang-Hyon Oh,In Sung Kim,Gwangil Kim,Jeong A Kim,Yang Soo Moon,장재철,이상석,Jong Hyun Jung,Jun Park,조광근 한국축산학회 2022 한국축산학회지 Vol.64 No.6
In this study, Rubus coreanus (R. coreanus) byproducts with high polyphenol content were fermented with R. coreanus-derived lactic acid bacteria (Lactobacillus plantarum GBL 16 and 17). Then the effect of R. coreanus-derived lactic acid bacteria fermented feed (RC-LAB fermented feed) with probiotics (Bacillus subtills, Aspergillus oryzae, Yeast) as a feed additive for pigs on the composition of intestinal microbes and the regulation of intestinal immune homeostasis was investigated. Seventy-two finishing Berkshire pigs were randomly allotted to four different treatment groups and 18 replicates. RC-LAB fermented feed with probiotics increased the genera Lactobacillus, Streptococcus, Mitsuokella, Prevotella, Bacteroides spp., Roseburia spp., and Faecalibacterium prausnitzii, which are beneficial bacteria of the digestive tract of pigs. Also, RC-LAB fermented feed with probiotics decreased the genera Clostridium, Terrisporobacter, Romboutsia, Kandleria, Megasphaera and Escherichia, which are harmful bacteria. In particular, the relative abundance of the genera Lactobacillus and Streptococcus increased by an average of 8.51% and 4.68% in the treatment groups and the classes Clostridia and genera Escherichia decreased by an average of 27.05% and 2.85% in the treatment groups. In mesenteric lymph nodes (MLN) and spleens, the mRNA expression of transcription factors and cytokines in Th1 and Treg cells increased and the mRNA expression of Th2 and Th17 transcription factors and cytokines decreased, indicating a regulatory effect on intestinal immune homeostasis. RC-LAB fermented feed regulates gut immune homeostasis by influencing the composition of beneficial and detrimental microorganisms in the gut and regulating the balance of Th1/Th2 and Th17/Treg cells.
Kim Da Seul,Park Jong Eun,황승연,Jeong Daun,Lee Guntak,Kim Taerim,Lee Se Uk,Yoon Hee,Cha Won Chul,심민섭,Jo Ik Joon,Shin Tae Gun 대한응급의학회 2022 Clinical and Experimental Emergency Medicine Vol.9 No.3
Objective We evaluated the performance of diastolic shock index (DSI) and lactate in predicting vasopressor requirement among hypotensive patients with suspected infection in an emergency department. Methods This was a single-center, retrospective observational study for adult patients with suspected infection and hypotension in the emergency department from 2018 to 2019. The study population was split into derivation and validation cohorts (70/30). We derived a simple risk score to predict vasopressor requirement using DSI and lactate cutoff values determined by Youden index. We tested the score by the area under the receiver operating characteristic curve (AUC). We performed a multivariable regression analysis to evaluate the association between the timing of vasopressor treatment and 28-day mortality. Results A total of 1,917 patients were included. We developed a score, assigning 1 point each for the high DSI (≥2.0) and high lactate (≥2.5 mmol/L) criteria. The AUCs of the score were 0.741 (95% confidence interval [CI], 0.715–0.768) at hypotension and 0.736 (95% CI, 0.708–0.763) after initial fluid challenge in the derivation cohort and 0.676 (95% CI, 0.631–0.719) at hypotension and 0.688 (95% CI, 0.642–0.733) after initial fluid challenge in the validation cohort, respectively. In patients with scores of 2 points, early vasopressor therapy initiation was significantly associated with decreased 28-day mortality (adjusted odds ratio, 0.37; 95% CI, 0.14–0.94). Conclusion A prediction model with DSI and lactate levels might be useful to identify patients who are more likely to need vasopressor administration among hypotensive patients with suspected infection.
Kim, Eun-Jung,Yoon, Ji-Young,Woo, Mi-Na,Kim, Cheul-Hong,Yoon, Ji-Uk,Jeon, Da-Nee The Korean Dental Society of Anesthsiology 2015 Journal of Dental Anesthesia and Pain Medicine Vol.15 No.2
In oral and maxillofacial surgery, many complications associated with nasotracheal tube can be caused. In this case, we reported ballooning tube damage of nasotracheal tube during orthognathic double-jaw surgery and replacement of tube through cut down of tube and tube exchange using airway exchange catheter. The patient scheduled for high Le Fort I osteotomy and bilateral sagittal split osteotomy was intubated nasotracheally with nasal endotracheal tube. During maxilla osteotomy, air bubble was detected in the oral blood. In spite of our repeated ballooning, the results were the same so we changed damaged tube using airway exchange catheter aseptically. Tiny and superficial cutting site was detected in the middle of pilot tube. As we know in our case, tiny injury impeded a normal airway management and prevention is important.
Kim, Woojin,Bae, Munjoo,Chang, Sei-Jin,Yoon, Jin-Ha,Jeong, Da Yee,Hyun, Dae-Sung,Ryu, Hye-Yoon,Park, Ki-Soo,Kim, Mi-Ji,Kim, Changsoo The Korean Society for Preventive Medicine 2019 예방의학회지 Vol.52 No.6
Objectives: It is well-known that post-traumatic stress disorder (PTSD) among firefighters contributes to their job-related stress. However, the relationship between burnout and PTSD in firefighters has rarely been studied. This study therefore explored the association between burnout and its related factors, such as trauma and violence, and PTSD symptoms among firefighters in Korea. Methods: A total of 535 firefighters participated in the Firefighter Research on Enhancement of Safety & Health study at 3 university hospitals from 2016 to 2017. The 535 participants received a baseline health examination, including questionnaires assessing their mental health. A Web-based survey was also conducted to collect data on job-related stress, history of exposure to violence, burnout, and trauma experience. The associations among burnout, its related factors, and PTSD symptoms were investigated using structural equation modeling. Results: Job demands (${\beta}=0.411$, p<0.001) and effort-reward balance (${\beta}=-0.290$, p<0.001) were significantly related to burnout. Burnout (${\beta}=0.237$, p<0.001) and violence (${\beta}=0.123$, p=0.014) were significantly related to PTSD risk. Trauma (${\beta}=0.131$, p=0.001) was significantly related to burnout; however, trauma was not directly associated with PTSD scores (${\beta}=0.085$, p=0.081). Conclusions: Our results show that burnout and psychological, sexual, and physical violence at the hands of clients directly affected participants' PTSD symptoms. Burnout mediated the relationship between trauma experience and PTSD.
Precise base editing without unintended indels in human cells and mouse primary myoblasts
Yoon Da Eun,Kim Na-Rae,Park Soo-Ji,Jeong Tae Yeong,Eun Bokkee,Cho Yongcheol,Lim Soo-Yeon,Lee Hyunji,Seong Je Kyoung,Kim Kyoungmi 생화학분자생물학회 2023 Experimental and molecular medicine Vol.55 No.-
Base editors are powerful tools for making precise single-nucleotide changes in the genome. However, they can lead to unintended insertions and deletions at the target sites, which is a significant limitation for clinical applications. In this study, we aimed to eliminate unwanted indels at the target sites caused by various evolved base editors. Accordingly, we applied dead Cas9 instead of nickase Cas9 in the base editors to induce accurate substitutions without indels. Additionally, we tested the use of chromatin-modulating peptides in the base editors to improve nucleotide conversion efficiency. We found that using both dead Cas9 and chromatin-modulating peptides in base editing improved the nucleotide substitution efficiency without unintended indel mutations at the desired target sites in human cell lines and mouse primary myoblasts. Furthermore, the proposed scheme had fewer off-target effects than conventional base editors at the DNA level. These results indicate that the suggested approach is promising for the development of more accurate and safer base editing techniques for use in clinical applications.
Effects of Rubus coreanus byproducts on intestinal microbiota and the immune modulation
Da Yoon Yu,Sung Hak Kim,Jeong A Kim,In Sung Kim,문양수,이상석,박화춘,Jong Hyun Jung,Yi Hyung Chung,Dae Keun Shin,남기창,In Soon Choi,조광근 아세아·태평양축산학회 2018 Animal Bioscience Vol.31 No.3
Objective: Although the efficacy of Rubus coreanus (RC) byproducts as a feed additive has been recognized, its effects on intestinal microorganisms and the immune system are still unknown. Methods: Six-week-old male rats were treated with 0.5% RC (T1), 1.0% RC (T2), and 1.5% RC (T3) for 4 weeks. Results: We found that treatment with RC byproducts significantly increased the daily gain of body weight and feed intake. Treg-cell differentiation was enhanced in the mesenteric lymph nodes and spleen from the rats fed with RC byproducts. Illumina sequencing showed that bacteria in the phylum Firmicutes decreased and while those in the phylum Bacteroidetes increased in RC-treated groups. Particularly, the pathogenic microorganisms in the family Peptococcaceae decreased, and the non-pathogenic families Lachnospiraceae and S24-7 increased. Quantitative polymerase chain reaction analysis showed that the RC byproducts increased the lactic acid bacteria Bifidobacterium spp., Oscillospira spp., Leuconostoc citreum, and Weissella cibaria in a concentration-dependent manner. Conclusion: RC byproducts may be effective in immunomodulation by affecting intestinal microorganisms.