Thermal and Mechanical Response of Soil and Tunnel during Replacement of Shield Tail Brush by Freezing Method

Xu-Yang Wang,Da-Jun Yuan,Da-Long Jin,Wei-Lin Su 대한토목학회 2020 KSCE JOURNAL OF CIVIL ENGINEERING Vol.24 No.5

In the long-distance shield tunneling process, the shield tail brush needs to be replaced after wear failure, especially in cross-river and cross-sea tunnel projects. Taking the shield tail brush replacement project of Qingchun Road tunnel in Hangzhou, China as the research background, a three-dimensional sequential thermo-mechanical coupled numerical simulation was carried out to study the shield tail brush replacement with liquid nitrogen freezing by ABAQUS software. The development of temperature fields and displacement fields of soil, and the deformations of the tunnel sections along the longitudinal direction induced by freezing were obtained. The numerical model was further validated by site measurement. In addition, the effects of the frozen wall thickness, buried depth, excavation radius, and coefficients of linear thermal expansion of soils on the frost heave were studied.

장백산 살충식물자원에 관한 연구

김대용(Da Yong Jin),여용석(Long Shi Lu),박금(Jin Piao),전설여(Xue Li Qian),여혜자(Hui Zi Lu) 강원대학교 농업과학연구소 2002 심포지움-생물자원의 보고 장백산 Vol.2002 No.-

후성유전학적 데이터를 활용한 고혈압 예측 딥러닝 모델

Thi Mai Nguyen(Thi Mai Nguyen ),Hoang Long Le(Hoang Long Le),Da Hae Kim(Da Hae Kim),Yun-Chul Hong(Yun-Chul Hong),Jin Hee Kim(Jin Hee Kim) 한국환경보건학회 2021 한국환경보건학회 학술대회 자료집 Vol.2021 No.2

Heavy concrete shielding properties for carbon therapy

Jin-Long Wang,Jiade J Lu,Da-Jun Ding,Wen-Hua Jiang,Ya-Dong Li,Rui Qiu,Hui Zhang,Xiao-Zhong Wang,Huo-Sheng Ruan,Yan-Bing Teng,Xiao-Guang Wu,Yun Zheng,Zi-Hao Zhao,Kai-Zhong Liao,Huan-Cheng Mai,Xiao-Dong Korean Nuclear Society 2023 Nuclear Engineering and Technology Vol.55 No.6

As medical facilities are usually built at urban areas, special concrete aggregates and evaluation methods are needed to optimize the design of concrete walls by balancing density, thickness, material composition, cost, and other factors. Carbon treatment rooms require a high radiation shielding requirement, as the neutron yield from carbon therapy is much higher than the neutron yield of protons. In this case study, the maximum carbon energy is 430 MeV/u and the maximum current is 0.27 nA from a hybrid particle therapy system. Hospital or facility construction should consider this requirement to design a special heavy concrete. In this work, magnetite is adopted as the major aggregate. Density is determined mainly by the major aggregate content of magnetite, and a heavy concrete test block was constructed for structural tests. The compressive strength is 35.7 MPa. The density ranges from 3.65 g/cm³ to 4.14 g/cm³, and the iron mass content ranges from 53.78% to 60.38% from the 12 cored sample measurements. It was found that there is a linear relationship between density and iron content, and mixing impurities should be the major reason leading to the nonuniform element and density distribution. The effect of this nonuniformity on radiation shielding properties for a carbon treatment room is investigated by three groups of Monte Carlo simulations. Higher density dominates to reduce shielding thickness. However, a higher content of high-Z elements will weaken the shielding strength, especially at a lower dose rate threshold and vice versa. The weakened side effect of a high iron content on the shielding property is obvious at 2.5 µSv=h. Therefore, we should not blindly pursue high Z content in engineering. If the thickness is constrained to 2 m, then the density can be reduced to 3.3 g/cm³, which will save cost by reducing the magnetite composition with 50.44% iron content. If a higher density of 3.9 g/cm³ with 57.65% iron content is selected for construction, then the thickness of the wall can be reduced to 174.2 cm, which will save space for equipment installation.

Identification and expression analysis of grape LRK10L-2 genes during grape fruit development

Ma Jin-Ping,Yin Xue-Ren,Wei Tong-Lu,Liu Hai-Nan,Pei Mao-Song,Yang Sheng-Di,Jin Hui-Ying,He Guang-Qi,Guo Da-Long 한국식물생명공학회 2022 Plant biotechnology reports Vol.16 No.1

LRK10L-2 is known to be related to the plant disease response, little information is available about the relationship of LRK10L-2 and fruit ripening. The protein physicochemical properties, conserved domains, gene structures, subcellular locali- zation, expression patterns during grape fruit development and promoter activity of the members of grape LRK10L-2 gene family were explored in this study. A total of 109 LRK10L-2 family gene members were identified, and mainly distributed on chromosome 16. Almost all of them were located in the plasma membrane. Most of the LRK10L-2 genes contain four or five motifs, ranging from 0 to 5 introns and have the cis-acting elements related to hormones in their promoter regions. There were 20 pairs of tandem duplicates and 293 pairs of segmental duplication in LRK10L-2 family genes. It was proved that the expression of LRK10L-2 gene varied at the different fruit development stages of 'Kyoho' and its early-ripening bud mutant, ‘Fengzao’. The subcellular localization of VIT_16s0098g00160 and VIT_16s0098g00400 were in the plasma membrane, and had a significant enrichment of the GUS signal in N.benthamiana leaves for the promoter. The results lay a solid basis for the further functional researches of the LRK10L-2 genes for grape fruit ripening.

Modeling and Simulation of the Magnetic Method for High Voltage Direct Current Inspection Robot

Xian-jin Xu,Cheng-hui Liu,Yu Yan,Hao-da Chen,Lei Fang,Yun-long Wang,Long-hui Wu 한국자기학회 2018 Journal of Magnetics Vol.23 No.3

In order to solve the slipping problem of a two-arm-wheel combined inspection robot, a maglev system based on the magnetic field of a high voltage direct current (HVDC) is proposed. The magnetic system comprises of two parts: a magnetic levitation system and a magnetic drive system. The levitation component overcomes the gravity of the robot by the Ampere force generated by current-carrying coils in the magnetic field of HVDC. The drive component utilizes the Ampere force generated by current-carrying coils in the magnetic field of HVDC as the driving force. Simulation results of the model are compared with the calculated values. The results show that the magnetic levitation method and the magnetic drive method are theoretically feasible, and the model is accurate and effective, which is of great practical significance to the physical realization of the inspection robot.

부동한 온도처리가 호랑나비의 생장발육에 주는 영향

여용석(Long Shi Lu),맹염령(Yan Ling Meng),김대용(Da Yong Jin) 강원대학교 농업과학연구소 2002 심포지움-생물자원의 보고 장백산 Vol.2002 No.-

Prognostic Significance of Overexpression of EZH2 and H3k27me3 Proteins in Gastric Cancer

He, Long-Jun,Cai, Mu-Yan,Xu, Guo-Liang,Li, Jian-Jun,Weng, Zi-Jin,Xu, Da-Zhi,Luo, Guang-Yu,Zhu, Sen-Lin,Xie, Dan Asian Pacific Journal of Cancer Prevention 2012 Asian Pacific journal of cancer prevention Vol.13 No.7

The enhancer of zeste homolog 2 (EZH2) methyl transferase and histone 3 lysine 27 (H3K27me3) protein can repress gene transcription, and their aberrant expression has been observed in various human cancers. This study determined their expression levels in gastric cancer tissues with reference to clinicopathological features and patient survival. We collected 117 gastric cancer and corresponding normal tissues for immunohistochemistry analysis. In gastric cancers, 82/117 (70.1%) were positive for EZH2 and 66/117 (56.4%) for H3K27me3 proteins in contrast to only 5.41% and 7.25% of normal gastric mucosa specimens, respectively. Kaplan-Meier survival data showed the average overall and disease-free survival of EZH2 high expression patients was 25.2 and 20.2 months, respectively, shorter than that with EZH2 low expression (40.5 and 35.9 months). The average overall survival and disease-free survival of high H3K27me3 expression patients was 23.4 and 17.4 months, shorter than without H3K27me3 expression (37.6 and 34.5 months). The average overall survival and disease-free survival of patients with both EZH2 and H3K27me3 expression was 18.8 and 12.9 months, respectively, shorter than that with either alone (34.7 and 31.2 months) or with low levels of both (43.9 and 39.9 months). Multivariate Cox regression analysis showed that H3K27me3 and EZH2 expression, tumor size differentiation and clinical stage were all independent prognostic factors for predicting patient survival. This study demonstrated that detection of both EZH2 and H3K27me3 proteins can predict poor survival of gastric cancer patients, superior to single protein detection. In addition, H3K27me3 and EZH2 protein expression could predict lymph node metastasis.

Physical properties and chemical composition of the cores in the California molecular cloud

Zhang, Guo-Yin,Xu, Jin-Long,Vasyunin, A. I.,Semenov, D. A.,Wang, Jun-Jie,Dib, Sami,Liu, Tie,Liu, Sheng-Yuan,Zhang, Chuan-Peng,Liu, Xiao-Lan,Wang, Ke,Li, Di,Wu, Zhong-Zu,Yuan, Jing-Hua,Li, Da-Lei,Gao, Springer-Verlag 2018 Astronomy and astrophysics Vol.620 No.-

<P><I>Aims.</I> We aim to reveal the physical properties and chemical composition of the cores in the California molecular cloud (CMC), so as to better understand the initial conditions of star formation.</P><P><I>Methods.</I> We made a high-resolution column density map (18.2′′) with <I>Herschel</I> data, and extracted a complete sample of the cores in the CMC with the fellwalker algorithm. We performed new single-pointing observations of molecular lines near 90 GHz with the IRAM 30m telescope along the main filament of the CMC. In addition, we also performed a numerical modeling of chemical evolution for the cores under the physical conditions.</P><P><I>Results.</I> We extracted 300 cores, of which 33 are protostellar and 267 are starless cores. About 51% (137 of 267) of the starless cores are prestellar cores. Three cores have the potential to evolve into high-mass stars. The prestellar core mass function (CMF) can be well fit by a log-normal form. The high-mass end of the prestellar CMF shows a power-law form with an index <I>α</I> = −0.9 ± 0.1 that is shallower than that of the Galactic field stellar mass function. Combining the mass transformation efficiency (<I>ε</I>) from the prestellar core to the star of 15 ± 1% and the core formation efficiency (CFE) of 5.5%, we suggest an overall star formation efficiency of about 1% in the CMC. In the single-pointing observations with the IRAM 30m telescope, we find that 6 cores show blue-skewed profile, while 4 cores show red-skewed profile. [HCO<SUP>+</SUP>]/[HNC] and [HCO<SUP>+</SUP>]/[N2H<SUP>+</SUP>] in protostellar cores are higher than those in prestellar cores; this can be used as chemical clocks. The best-fit chemical age of the cores with line observations is ~5 × 10<SUP>4</SUP> yr.</P>

Mesenchymal-to-epithelial transition of osteoblasts induced by Fam20c knockout

Geng Ya-Wei,Zhang Zhen,Jin Han,Da Jun-Long,Zhang Kai,Wang Jian-Qun,Guo Yu-Yao,Zhang Bin,Li Ying 한국유전학회 2022 Genes & Genomics Vol.44 No.2

Background: Fam20c is intimately related to tissue development and diseases. At present, it has been reported that Fam20c regulates the mineralization of osteoblasts, but there are few reports on other effects. Objective: To study the effect of Fam20c on osteoblasts by knocking out the Fam20c gene. Methods: Fam20c knockout osteoblasts were constructed by transfecting mouse osteoblasts with lentivirus. The proliferation, migration and mineralization of Fam20c knockout cells were detected by CCK-8, scratch test and alizarin red staining assays. The subcellular structure was observed by transmission electron microscopy. RT-PCR was used to detect the differential expression of mesenchymal-to-epithelial transition (MET)-related marker genes and core transcription factors. The differential expression of MET-related proteins was detected by immunofluorescence or Western blot. Transcriptome analysis of Fam20c knockout osteoblasts was performed, and real-time PCR was used to verify transcriptome analysis related to MET. Results: The proliferation ability of osteoblasts was not significantly changed after Fam20c deletion, but the migration ability and mineralization ability were significantly weakened. There were tight junctions between Fam20c knockout cells. The expression of mesenchymal cell marker genes and core transcription factors was significantly decreased, and the expression of epithelial cell marker genes was significantly increased. The expression of mesenchymal cell marker proteins was significantly decreased, and the expression of epithelial cell marker proteins was significantly increased. Multiple signalling molecules and pathways involved in MET have changed. Conclusions: Knockdown of Fam20c resulted in MET. Fam20c affects the transcription of key factors in osteoblast MET.