Mechanism of E2F1 in the proliferation, migration, and invasion of endometrial carcinoma cells via the regulation of BMI1 transcription

Lu Yanyang,Wei Ying,Shen Xiaoqin,Tong Yixi,Lu Jin,Zhang Yahui,Ma Yun,Zhang Rong 한국유전학회 2023 Genes & Genomics Vol.45 No.11

Background Endometrial carcinoma (EC) is the most prevalent gynecological cancer. Transcription factor (TF) regulates a large number of downstream target genes and is a key determinant of all physiological activities, including cell proliferation, differentiation, apoptosis, and cell cycle. The transcription factor E2F1 shows prominent roles in EC. BMI1 is a member of Polycomb suppressor Complex 1 (PRC1) and has been shown to be associated with EC invasiveness. It is currently unclear whether E2F1 can participate in the proliferation, migration, and invasion processes of EC cells by regulating BMI1 transcription. Objective We investigated whether E2F1 could participate in the proliferation, migration, and invasion processes of EC cells by regulating BMI1 transcription, in order to further clarify the pathogenesis and etiology of EC, and provide reference for identifying potential therapeutic targets and developing effective prevention and treatment strategies for this disease. Methods Human endometrial epithelial cells (hEECs) and human EC cell lines were selected. E2F1 expression was assessed by Western blot. E2F1 was silenced in AN3CA or overexpressed in HEC-1 by transfections, or E2F1 was silenced and BMI1 was overexpressed in AN3CA by cotransfection. Cell proliferation, migration, and invasion were detected by MTT, wound healing, and Transwell assays. The binding sites between E2F1 and BMI1 promoters were predicted through JASPAR website, and the targeted binding was verified by dual-luciferase report and ChIP assays. Results E2F1 was up-regulated in human EC cell lines, with its expression highest in AN3CA, and lowest in HEC-1. AN3CA invasion, migration, and proliferation were repressed by E2F1 knockdown, while those of HEC-1 cells were promoted by E2F1 overexpression. E2F1 overexpression increased the activity of wild type BMI1 reporter vector promoter, while this promotion was weakened after mutation of the predicted binding site in the BMI1 promoter. In the precipitated E2F1, BMI1 promoter site level was higher than that of IgG immunoprecipitant. BMI1 silencing suppressed AN3CA cell growth. BMI1 overexpression partially abrogated E2F1 silencing-inhibited EC cell growth. Conclusion E2F1 promoted EC cell proliferation, invasion, and migration by promoting the transcription of BMI1.

Finger Vein Representation by Modified Binary Tree Model

Tong Liu,Jianbin Xie,Huanzhang Lu,Wei Yan,Peiqin Li 한국산학기술학회 2013 SmartCR Vol.3 No.2

Finger vein recognition has high identification accuracy and strong security performance, which can be used in banks, offices, factories, etc. Although image representation is not a necessary process for finger vein recognition, a proper representation method can help to explore distribution regularities and structure differences of finger veins, and provides instructive information for finger vein recognition. It is very difficult to represent finger veins because of their irregular structure. Therefore, four principles (caliber uniformity, node replication, loop splitting, and virtual connection) are proposed in this paper, first to simplify the finger vein structure as a binary tree structure. Then a modified binary tree model is proposed based on the binary tree structure. The new model uses the binary tree to describe the relationships between different vein branches and uses a B-spline function to describe the spatial structure of vein branches. Experiments show that this model can quantitatively describe the relationships between, and the spatial structure of, vein branches with little representation error and low storage space requirements.

Nonlinear identification of Bouc–Wen hysteretic parameters using improved experience-based learning algorithm

Wei-Li Luo,Tongyi Zheng,Huawei Tong,Yun Zhou,Zhongrong Lu 국제구조공학회 2020 Structural Engineering and Mechanics, An Int'l Jou Vol.76 No.1

In this paper, an improved experience-based learning algorithm (EBL), termed as IEBL, is proposed to solve the nonlinear hysteretic parameter identification problem with Bouc-Wen model. A quasi-opposition-based learning mechanism and new updating equations are introduced to improve both the exploration and exploitation abilities of the algorithm. Numerical studies on a single-degree-of-freedom system without/with viscous damping are conducted to investigate the efficiency and robustness of the proposed algorithm. A laboratory test of seven lead-filled steel tube dampers is presented and their hysteretic parameters are also successfully identified with normalized mean square error values less than 2.97%. Both numerical and laboratory results confirm that, in comparison with EBL, CMFOA, SSA, and Jaya, the IEBL is superior in nonlinear hysteretic parameter identification in terms of convergence and accuracy even under measurement noise.

Intravenous Tenecteplase for Acute Ischemic Stroke Within 4.5–24 Hours of Onset (ROSE-TNK): A Phase 2, Randomized, Multicenter Study

Wang Lu,Dai Ying-Jie,Cui Yu,Zhang Hong,Jiang Chang-Hao,Duan Ying-Jie,Zhao Yong,Feng Ye-Fang,Geng Shi-Mei,Zhang Zai-Hui,Lu Jiang,Zhang Ping,Zhao Li-Wei,Zhao Hang,Ma Yu-Tong,Song Cheng-Guang,Zhang Yi,Ch 대한뇌졸중학회 2023 Journal of stroke Vol.25 No.3

Background and Purpose Intravenous tenecteplase (TNK) efficacy has not been well demonstrated in acute ischemic stroke (AIS) beyond 4.5 hours after onset. This study aimed to determine the effect of intravenous TNK for AIS within 4.5 to 24 hours of onset. Methods In this pilot trial, eligible AIS patients with diffusion-weighted imaging (DWI)-fluid attenuated inversion recovery (FLAIR) mismatch were randomly allocated to intravenous TNK (0.25 mg/kg) or standard care within 4.5–24 hours of onset. The primary endpoint was excellent functional outcome at 90 days (modified Rankin Scale [mRS] score of 0–1). The primary safety endpoint was symptomatic intracranial hemorrhage (sICH). Results Of the randomly assigned 80 patients, the primary endpoint occurred in 52.5% (21/40) of TNK group and 50.0% (20/40) of control group, with no significant difference (unadjusted odds ratio, 1.11; 95% confidence interval 0.46–2.66; <i>P</i>=0.82). More early neurological improvement occurred in TNK group than in control group (11 vs. 3, <i>P</i>=0.03), but no significant differences were found in other secondary endpoints, such as mRS 0–2 at 90 days, shift analysis of mRS at 90 days, and change in National Institutes of Health Stroke Scale score at 24 hours and 7 days. There were no cases of sICH in this trial; however, asymptomatic intracranial hemorrhage occurred in 3 of the 40 patients (7.5%) in the TNK group. Conclusion This phase 2, randomized, multicenter study suggests that intravenous TNK within 4.5–24 hours of onset may be safe and feasible in AIS patients with a DWI-FLAIR mismatch.

Overcoming 5-Fu Resistance of Colon Cells through Inhibition of Glut1 by the Specific Inhibitor WZB117

Liu, Wei,Fang, Yong,Wang, Xiao-Tong,Liu, Ju,Dan, Xing,Sun, Lu-Lu Asian Pacific Journal of Cancer Prevention 2014 Asian Pacific journal of cancer prevention Vol.15 No.17

Background: 5-Fluorouracil (5-FU) is the most commonly used drug in colon cancer therapy. However, despite impressive clinical responses initially, development of drug resistance to 5-Fu in human tumor cells is the primary cause of failure of chemotherapy. In this study, we established a 5-Fu-resistant human colon cancer cell line for comparative chemosensitivity studies. Materials and Methods: Real time PCR and Western blotting were used to determine gene expression levels. Cell viability was measured by MTT assay. Glucose uptake was assess using an Amplex Red Glucose/Glucose Oxidase assay kit. Results: We found that 5-Fu resistance was associated with the overexpression of Glut1 in colon cancer cells. 5-Fu treatment at low toxic concentration induced Glut1 expression. At the same time, upregulation of Glut1 was detected in 5-Fu resistant cells when compared with their parental cells. Importantly, inhibition of Glut1 by a specific inhibitor, WZB117, significantly increased the sensitivity of 5-Fu resistant cells to the drug. Conclusions: This study provides novel information for the future development of targeted therapies for the treatment of chemo-resistant colon cancer patients. In particular it demonstrated that Glut1 inhibitors such as WZB117 may be considered an additional treatment options for patients with 5-Fu resistant colon cancers.

Failure characteristics of combined coal-rock with different interfacial angles

Zhao, Tong-Bin,Guo, Wei-Yao,Lu, Cai-Ping,Zhao, Guang-Ming Techno-Press 2016 Geomechanics & engineering Vol.11 No.3

In order to investigate the influence of the interfacial angel on failure characteristics and mechanism of combined coal-rock mass, 35 uniaxial/biaxial compressive simulation tests with 5 different interfacial angels of combined coal-rock samples were conducted by PFC2D software. The following conclusions are drawn: (1) The compressive strength and cohesion decrease with the increase of interfacial angle, which is defined as the angle between structure plane and the exterior normal of maximum principal plane, while the changes of elastic modulus and internal friction angle are not obvious; (2) The impact energy index $K_E$ decreases with the increase of interfacial angle, and the slip failure of the interface can be predicted based on whether the number of acoustic emission (AE) hits has multiple peaks or not; (3) There are four typical failure patterns for combined coal-rock samples including I (V-shaped shear failure of coal), II (single-fracture shear failure of coal), III (shear failure of rock and coal), and IV (slip rupture of interface); and (4) A positive correlation between interfacial angle and interface effect is shown obviously, and the interfacial angle can be divided into weak-influencing scope ($0-15^{\circ}$), moderate-influencing scope ($15-45^{\circ}$), and strong-influencing scope (> $45^{\circ}$), respectively. However, the confining pressure has a certain constraint effect on the interface effect.

Construction of a genetic linkage map and QTL mapping of agronomic traits in Auricularia auricula-judae

Li-Xin Lu,Fang-Jie Yao,Peng Wang,Ming Fang,You-Min Zhang,Wei-Tong Zhang,Xiang-Hui Kong,Jia Lu 한국미생물학회 2017 The journal of microbiology Vol.55 No.10

Auricularia auricula-judae is a traditional edible fungus that is cultivated widely in China. In this study, a genetic linkage map for A. auricula-judae was constructed using a mapping population consisting of 138 monokaryons derived from a hybrid strain (A119-5). The monokaryotic parent strains A14-5 and A18-119 were derived from two cultivated varieties, A14 (Qihei No. 1) and A18 (Qihei No. 2), respectively. In total, 130 simple sequence repeat markers were mapped. These markers were developed using the whole genome sequence of A. auricula-judae and amplified in A14-5, A18- 119, and the mapping population. The map consisted of 11 linkage groups (LGs) spanning 854 cM, with an average interval length of 6.57 cM. A testcross population was derived from crossing between the monokaryon A184-57 (from the wild strain A184 as a tester strain) and the mapping population. Important agronomic trait-related QTLs, including mycelium growth rate on potato dextrose agar for the mapping population, mycelium growth rate on potato dextrose agar and sawdust for the testcross population, growth period (days from inoculation to fruiting body harvesting), and yield for the testcross population, were identified using the composite interval mapping method. Six mycelium growth raterelated QTLs were identified on LG1 and LG4, two growth period-related QTLs were identified on LG2, and three yieldrelated QTLs were identified on LG2 and LG6. The results showed no linkage relationship between mycelium growth rate and growth period. The present study provides a foundation for locating genes for important agronomic characteristics in A. auricula-judae in the future.

The Antibacterial Mechanism of Zn(II) Frame Supported on Alginate Membrane

Dan Luo,Ruo-Wei Lu,Cui-Juan Wang,Yan Tong,Cheng Liu,Yu-Mei Xiao,Yan-Xia Chen 대한화학회 2020 Bulletin of the Korean Chemical Society Vol.41 No.11

In order to solve the problem of antibiotic-resistant bacteria caused by excessive use of antibiotics, herein, an antibacterial membrane composed of natural sodium alginate (ALG), zeolite imidazolate skeleton (ZIF-8) and niflumic acid (NIF) was reported. The membrane serves as a versatile platform for local antibacterial. This report carried out in-depth research on the physical properties and antibacterial mechanism of the synthesized sodium alginate composite film. The data shows that the sodium alginate-based antibacterial film has a continuous antibacterial effect, and the release of antibacterial molecules can be controlled according to changes in the external environment. The results show that the complex has stronger mechanical and bacteriostatic properties. Niflumic acid and Zn(II) have synergistic antibacterial effect. The complex promotes bacterial death by hindering bacterial respiratory metabolism and destroying cell membrane structure.

Effect of different tooth preparation designs on the marginal and internal fit discrepancies of cobalt-chromium crowns produced by computer-aided designing and selective laser melting processes

Na Yu,Hong-Wei Dai,Fa-Bing Tan,Jin-Lin Song,Chao-Yi Ma,Xue-Lu Tong 대한치과보철학회 2021 The Journal of Advanced Prosthodontics Vol.13 No.5

PURPOSE. To evaluate the impact of five different tooth preparation designs on the marginal and internal fit discrepancies of cobalt-chromium (CoCr) crowns produced by computer-aided designing (CAD) and selective laser melting (SLM) processes. MATERIALS AND METHODS. Five preparation data were constructed, after which design crowns were obtained. Actual crowns were fabricated using an SLM process. After the data of actual crowns were obtained with structural light scanning, intaglio surfaces of the design crown and actual crown were virtually superimposed on the preparation. The fit-discrepancies were displayed with colors, while the root means square was calculated and analyzed with one-way analysis of variance (ANOVA), Tukey’s test or Kruskal-Wallis test (α = .05). RESULTS. The marginal or internal color-coded images in the five design groups were not identical. The shoulder-lip and sharp line angle groups in the CAD or SLM process had larger marginal or internal fit discrepancies compared to other groups (P < .05). In the CAD process, the mean marginal and internal fit discrepancies were 10.0 to 24.2 μm and 29.6 to 31.4 μm, respectively. After the CAD and SLM processes, the mean marginal and internal fit discrepancies were 18.4 to 40.9 μm and 39.1 to 47.1 μm, respectively. The SLM process itself resulted in a positive increase of the marginal (6.0 - 16.7 μm) and internal (9.0 - 15.7 μm) fit discrepancies. CONCLUSION. The CAD and SLM processes affected the fit of CoCr crowns and varied based on the preparation designs. Typically, the shoulder-lip and sharp line angle designs had a more significant effect on crown fit. However, the differences between the design groups were relatively small, especially when compared to fit discrepancies observed clinically.

Directional production of even carbon volatile fatty acids from peanut meal: effects of initial pH and hydraulic residence time

Yujia Chen,Qian Fang,Lu Zhang,Kequan Zhang,Tong Wei,Qiqi Ping 대한환경공학회 2022 Environmental Engineering Research Vol.27 No.4

In this study, peanut meal was used as the substrate for the continuous production of even carbon volatile fatty acids (ECFA) through anaerobic fermentation. First, a continuous stirred reactor (CSTR) was started by adjusting the initial pH to acclimate the related bacteria, and then the hydraulic residence time (HRT) was optimized. The effects of different initial pH and HRT on the potential of ECFA production by peanut meal fermentation, the transformation and utilization of main organic compounds, and the composition and replacement of microbial community structure behind it were investigated. The results showed that ECFA was the dominant factor in the acid production of peanut meal fermentation, and alkaline pH and HRT = 6 d were the more favorable operating conditions for the hydrolysis and acidification of peanut meal. The average concentration of total volatile fatty acids (TVFAs) obtained was 15,249.50 mg/L, and the content of ECFA varied from 88.51% to 96.27%, with an average ratio of 92.55%. This operation condition drove the formation of microbial community structure, mainly including Prepotella, Tepididimicrobium, Anaerosalibacter, and Igatzschineria, which were favorable for ECFA production, reflecting the potential of using peanut meal for targeted production of ECFA.