Development of an Automated Image Analyzer for Microvessel Density Measurement in Bone Marrow Biopsies

Yousun Chung,Seungwon Shin,Hyoeun Shim,Ji Yeon Sohn,Dong-eun Lee,Lee Hyewon,Hyeon Seok Eom,Kwang Gi Kim,Sun-Young Kong 대한진단검사의학회 2020 Annals of Laboratory Medicine Vol.40 No.4

Angiogenesis is important for the proliferation and survival of multiple myeloma (MM) cells. Bone marrow (BM) microvessel density (MVD) is a useful marker of angiogenesis and an increase in MVD can be used as a marker of poor prognosis in MM patients. We developed an automated image analyzer to assess MVD from images of BM biopsies stained with anti-CD34 antibodies using two color models. MVD was calculated by merging images from the red and hue channels after eliminating non-microvessels. The analyzer results were compared with those obtained by two experienced hematopathologists in a blinded manner using the 84 BM samples of MM patients. Manual assessment of the MVD by two hematopathologists yielded mean±SD values of 19.4±11.8 and 20.0±11.8. The analyzer generated a mean±SD of 19.5±11.2. The intraclass correlation coefficient (ICC) and Bland-Altman plot of the MVD results demonstrated very good agreement between the automated image analyzer and both hematopathologists (ICC=0.893 [0.840–0.929] and ICC=0.906 [0.859–0.938]). This automated analyzer can provide time- and labor-saving benefits with more objective results in hematology laboratories.

Manifestation of Authoritarian Resilience?:

Yousun Chung 서울대학교 국제학연구소 2017 Journal of International and Area Studies Vol.24 No.1

This article discusses how middle-class homeowners in urban China have made advancement in collective interest representation and thus protection of private property rights in more systematic ways: not just by contending but by participating in public policy process. This is quite salient in their participation in relevant legislation, from the Property Law in 2007 to the Ordinance on Property Management in Beijing in 2010 and a new version of property management-related local legislation underway. Further investigation of this topic sheds lights on theoretical issues of in what ways and to what extents the state-society interaction in contemporary China is evolving.

A Pilot Project for the Expansion of the External Quality Assessment Program in Transfusion Medicine: Anti-D Titer Test

Yousun Chung,Jin Seok Kim,육희정,Sang-Hyun Hwang,Heung-Bum Oh,Hyungsuk Kim,Dae-Hyun Ko 대한임상검사정도관리협회 2022 Journal of Laboratory Medicine And Quality Assuran Vol.44 No.2

Background: Many test items in transfusion medicine that are still not included in the Korean Association of External Quality Assessment Service. We performed a pilot project of anti-D titer test for the expansion of proficiency testing program in transfusion medicine. Methods: Proficiency testing materials with low (BBS2-21-01) and high (BBS2-21-02) anti-D titer were sent to 53 laboratories that agreed to participate in the pilot project for the 2nd Korean Association of External Quality Assessment Service on Transfusion Medicine in 2021. The results were entered on the website of the Korean Association of External Quality Assessment Service (http://eqas.keqas.org/). Results: A total of 47 laboratories submitted the results. For BBS2-21-01, the intended response of anti-D titer was 4–16 for tube technique with antihuman globulin (AHG) and 16–64 for column agglutination technique (CAT) with AHG. Meanwhile, for BBS2-21-02, the intended response was 8–32 for tube technique with AHG and 32–128 for CAT with AHG. The proportion of acceptable results was 90.9%–95.5% for tube technique with AHG and 95.8%–100.0% for CAT with AHG. Conclusions: The pilot project of anti-D titer test was successfully performed. This project will be expanded to a biannual proficiency testing program for anti-D antibody titer in the Korean Association of External Quality Assessment Service starting in 2022.

In Search of Peace Studies in China: The Rise of Rights Movements, Conflict Resolution, and Prospects of Peace Studies

( Yousun Chung ) 서울대학교 통일평화연구원 2023 Asian Journal of Peacebuilding Vol.11 No.1

Peace studies in China has had meaningful initial developments, but the sustainability of the field’s development has been constrained by an authoritarian political atmosphere. Rights and justice are core issues in peace studies, but these remain unaddressed in China. Thus, this article highlights studies on rights movements as de facto peace studies in an authoritarian setting and compares the movement among the middle class and that of peasants/workers, which can help us understand current contradictions and disharmony in Chinese society. Maintaining the stability of a turbulent society is becoming a crucial agenda for the Chinese state. Accordingly, studies on rights movements will become increasingly important.

Molecular Analysis of Eight American Type Culture Collection Gonococcal Strains by Neisseria gonorrhoeae Multiantigen Sequence Typing and PorB Sequence Typing

Yousun Chung,Minje Han,박지영,Sora Kang,Inhee Kim,Jung A Park,Jae-Seok Kim 대한임상검사정도관리협회 2019 Journal of Laboratory Medicine And Quality Assuran Vol.41 No.1

Background: Molecular epidemiological typing of Neisseria gonorrhoeae is crucial for monitoring the spread of resistant strains. As reference strains can be used for laboratory internal quality control, we genetically characterised the American Type Culture Collection (ATCC) gonococcal strains by Neisseria gonorrhoeae multiantigen sequence typing (NGMAST) and porB sequence typing using public multilocus sequence typing (PubMLST). Methods: Eight ATCC gonococcal reference strains (ATCC 19424, ATCC 31426, ATCC 35541, ATCC 43069, ATCC 43070, ATCC 49226, ATCC 49926, and ATCC 49981) from Culti-Loops (Thermo Fisher Scientific, USA) were cultured. After DNA extraction, porB and tbpB were amplified and sequenced. Sequence types (STs) and allele numbers were each determined by NG-MAST (http://www.ng-mast.net) and porB sequence typing using PubMLST (http://pubmlst.org/neisseria/porB/). Results: ATCC 19424 was identified as ST 266 by NG-MAST, and as Allele 946 by PubMLST. ATCC31426 was assigned a novel ST by NG-MAST, and was assigned Allele 958 with 1.2% mismatch by PubMLST. ATCC 35541 was identified as ST 12 by NG-MAST, and as Allele 624 by PubMLST. ATCC 43069 and ATCC 43070 were both identified as ST 681 by NGMAST, and as Allele 984 by PubMLST. ATCC 49226 was identified as ST 1572 by NG-MAST, and as Allele 2110 by PubMLST. ATCC 49926 and ATCC 49981 were both identified as ST 16496 by NG-MAST, and as Allele 928 by PubMLST. Conclusions: The ST data obtained for ATCC gonococcal reference strains by NG-MAST and porB sequence typing using PubMLST can be used for quality assurance of molecular epidemiological typing in clinical microbiological laboratories.

A Pilot Project for Expansion of the External Quality Assessment Program in Transfusion Medicine: Extended Antigen Typing

Yousun Chung,Jin Seok Kim,John Jeongseok Yang,Hyungsuk Kim,Sang-Hyun Hwang,Heung-Bum Oh,고대현 대한임상검사정도관리협회 2021 Journal of Laboratory Medicine And Quality Assuran Vol.43 No.2

Background: As the need for expansion of the external quality assessment (EQA) scheme for transfusion medicine in Korea has increased, we performed a pilot project of extended antigen typing for Lewis, Duffy, and Kidd antigens. Methods: We sent EQA materials to participant laboratories for Lewis, Duffy, and Kidd antigen typing in October 2020. Participants were asked to enter the agglutination titers, methodologies, and results of Lewis, Duffy, and Kidd antigen typing on the Korean Association of External Quality Assessment Service website. Results: For Lewis antigen typing, the intended result was Le(a–b–); 20 participants (95.2%) responded as Le(a–b–), and only one (4.8%) responded as Le(a–b+). For the Duffy antigen typing test, the intended result was Fy(a+b–), and all 20 participants (100%) responded as Fy(a+b–). For the Kidd antigen typing test, the intended result was Jk(a+b+), and all 22 participants (100%) responded as Jk(a+b+). Conclusions: As the pilot project was successfully executed, extended antigen typing for Lewis, Duffy, and Kidd antigens has been included in the Korean Association of External Quality Assessment Service on transfusion medicine since 2021.

Choice of ABO Group for Blood Component Transfusion in ABO-Incompatible Solid Organ Transplantation: A Questionnaire Survey in Korea and Guideline Proposal

Chung Yousun,Ko Dae-Hyun,Lim Jihyang,Kim Kyeong-Hee,Kim Hyungsuk 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.1

The number of ABO-incompatible solid organ transplantations (ABOi SOTs) has markedly increased worldwide since the early 2000s. We investigated the choice of ABO group for blood component transfusion in ABOi SOT. We conducted a survey by e-mailing a questionnaire to blood bank specialists at 77 major hospitals in Korea, among whom 34 responded to the survey. In major ABOi SOT, for red blood cells (RBCs), the recipient’s type (70.6%) was the most common choice, followed by group O (29.4%); for platelets, group AB (50.0%) was the most common choice, followed by the donor type (38.2%); for plasma, group AB (55.9%) was the most common choice, followed by the donor type (32.4%). In bidirectional ABOi SOT, for RBCs, the recipient’s type (55.9%) was the most common choice, followed by group O (44.1%); for platelets and plasma, group AB was the most common choice (94.1% and 97.1%, respectively). The policies for transfusion in ABOi SOT were diverse. We suggest a guideline on the choice of ABO group for transfusion in ABOi SOT to secure patient health and enable an efficient use of blood components.

Proposal of the Need for New Korean Guidelines on the Use of Therapeutic Apheresis in Clinical Practice

Chung Yousun,Kim Hyungsuk,Ko Dae-Hyun 대한진단검사의학회 2022 Annals of Laboratory Medicine Vol.42 No.6

타이상(臺商) 연구의 현황과 동향 고찰

鄭有善 ( Chung Yousun ),鄭善旭 ( Chung Sun Wook ),張榮熙 ( Chang Young Hee ) 현대중국학회 2020 現代中國硏究 Vol.22 No.3

타이상(臺商)은 경제·경영, 정치, 사회·문화 등 여러 측면에서 흥미로운 연구 질문들을 던져왔고, 이를 반영하여 해외 학계에서는 1990년대 중반 이후 타이상 연구가 본격화되고 다양한 연구들이 소개되었다. 이러한 배경 하에 본 연구는 타이상 연구의 현황과 동향을 고찰하여 국내 학계에 소개하고 향후 연구 과제를 제언하고자 한다. 본 논문은 2000년대 이후 소개된 타이상 연구에 초점을 두어 중화권(주로 대만과 중국)과 서구에서 발표된 연구들을 크게 경제·경영, 사회, 정치 분야로 구분해 고찰했다. 경제 및 경영 분야에서는 타이상의 중국 진출 동기, 진출 지역, 투자의 변화 양상, 타이상과 지방정부의 관계, 미중 갈등이 타이상에 미치는 영향등으로 분류하여 최근 연구들을 조명했다. 사회 분야에서는 타이상의 정체성 변화, 타이상과 중국사회에서의 적응 및 통합, 타이상과 중국인 노동자 간의 노사 문제 등을 중심으로 진행된 여러 연구들을 검토하고, 향후 타이상 연구가 사회학적 학문성을 갖기 위해 이민 연구 및 계급 정치적 접근에 초점을 맞추게 될 것임을 전망했다. 정치 분야에서는 경제적 협력과 정치적 갈등이 복합적으로 얽혀있는 양안관계의 상황 속에서 타이상의 정치적 영향에 초점을 맞춘 연구들을 고찰했다. 타이상이 중국 정치, 대만정치, 양안 관계 각각에 미치는 영향으로 세분화해 그 정치적 영향성을 조명했다. 결론 부분에서는 타이상 연구가 중국 진출 한상(韓商)에게 제시하는 시사점을 논의하고 이와 관련된 향후 비교연구 과제를 제언하였다. The roles and activities of Taishang (Taiwanese business people) have so far provided intriguing research questions from economic, political, social and cultural aspects, and thus received growing academic attention since the mid-1990s. This article aims to introduce the trend of Taishang studies to the Korean academic community and make suggestions for future research. Our main focus of analysis is the recent Taishang studies published in the post-2000 period in mainland China, Taiwan, and the West. We categorize existing Taishang studies into three categories: economy/business, society, and politics. In economy/business domain, entry strategy and its changes, the relationship between Taishang and Chinese local government, and the impact of the recent tension between China and US on Taishang have drawn scholarly attention. In society domain, scholars have focused on changing identities of Taishang, Taishang’s adaption and assimilation to the Chinese society, and labor-management relations between Taishang and local Chinese workers. Future research needs to analyze these themes from the angle of immigration and class. In politics domain, Taishang’s role as political actors in the midst of intricate cross-strait relations has been highlighted. The Taishang’s political agency has been discussed from various dimensions such as Taishang’s influence on the Chinese politics, Taiwanese politics, and cross-strait relations. In conclusion, we discuss the implications Taishang studies provides to the studies of Hanshang (Korean business people) who have moved to China over the last three decades, and suggest that more scholarly attention be paid to the comparative study of Taishang and Hanshang.

대만 태양화 운동 연구: 메커니즘 접근법을 중심으로

정유선 ( Chung Yousun ) 한국외국어대학교 중국연구소 2016 中國硏究 Vol.68 No.-

Judging from what it has accomplished, the Sunflower movement in Taiwan in spring 2014 is a relatively successful social movement. It has deterred speedy passage of Cross-Straits Service Trade Agreement intended by the KMT government, and triggered massive change of political landscape in Taiwan. As a social movement, through which processes was the Sunflower movement able to induce relatively successful outcome? Based on "mechanism-based approach", this article analyzes the case of Sunflower movement through three mechanisms: environmental, cognitive, and relational. This work puts forward a comprehensive approach to the structure-agency problem in social movement studies, while transcending the conventional efforts to find causal relations between individual variables.