HBV : Dynamic Change of HBsAg Titer during Anti-Viral Therapy in Treatment-Na?ve Patients with Chronic Hepatitis B

( Han Wook Chung ),( Yu Jin Ko ),( Sang Wook Lee ),( Hyun Woong Lee ),( Hyung Joon Kim ) 대한간학회 2013 춘·추계 학술대회 (KASL) Vol.2013 No.1

Background/Aims: Quantitative HBsAg (qHBsAg) titers are emerging as useful tools for measuring viral load and predicting predicting virologic response (VR). This study aimed to determine whether serial monitoring of HBsAg levels could predict virologic response to entecavir(ETV) & clevudine(CLV) therapy in treatment-naive patients with CHB Methods: A total of 157 treatment-naive patients with CHB, who visited Chung-Ang University Hospital between January 2007 and December 2008, were enrolled in this study (100 males). 97 patients(69 males, mean age 48) were treated with ETV for average 53 months, and 60 patients (31 males, mean age 43) were treated with CLV for 26months average. HBsAg levels were measured at baseline, week 12, 24, and 48. All patients were assessed for virological response (HBV DNA<140 copies/mL). Serum HBV DNA was quantified using the real time PCR assay (Artus HBV LC PCR Kit, Roche Diagnostics, lower limit of quantification, 140 copies/mL). Results: In 97 ETV-treated patients, the rates of virological response were 56.7% (55/97) at 48months and 60 CLV-treated patients, 30% (18/60) at 24 months, respectively. Mean reduction of baseline HBV DNA was -5.3, and -4.9 log10 copies/mL. In 55 VR patients treated with ETV, 43(78.1%) patients showed early decline of HBsAg titer and 31(56.3%) patients had lower baseline HBsAg levels (< 3.7 log IU/mL). In 18 VR patients treated with CLV, 9(50%) patients showed early decline of HBsAg titer and 11(61.1%) patients had also lower baseline HBsAg levels (< 3.7 log IU/mL) . Conclusions: Early decline of HBsAg was useful for predicting VR, and baseline HBsAg could be indicative as well in CHB patients treated with ETV than patients treated with CLV.

형질전환을 위한 청치마의 재분화 체계확립

박태성(Tae Sung Park),조동욱(Dong Wook Cho),진명호(Myung Ho Jean),정규환(Kyu Hwan Chung) 한국원예학회 2000 원예과학기술지 Vol.18 No.5

Association of <i>SNCA</i> variants with α-synuclein of gastric and colonic mucosa in Parkinson's disease

Chung, Sun Ju,Kö,nig, Inke R.,Lohmann, Katja,Hinrichs, Frauke,Kim, Juyeon,Ryu, Ho-Sung,Lee, Hyo Jeong,Kim, Kiju,Lee, Jeong Hoon,Jung, Kee Wook,Kim, Mi Jung,Kim, Mi-Jung,Kim, Young Jin,Yun, Sung-Ch Elsevier 2019 Parkinsonism & related disorders Vol.61 No.-

<P><B>Abstract</B></P> <P><B>Background</B></P> <P>Alpha-synuclein (α-Syn) immunostaining in the enteric nervous system (ENS) has been investigated to determine the role of diagnostic biomarker of Parkinson's disease (PD). However, determining factors for alpha-synuclein (α-Syn) deposition in the ENS of humans are still unclear. We aimed to investigate a possible association between <I>SNCA</I> variants and the presence of α-Syn immunostaining in the ENS in patients with PD and healthy individuals.</P> <P><B>Methods</B></P> <P>The study subjects consisted of 38 patients with PD and 46 healthy individuals. α-Syn immunohistochemistry was performed for gastric and colonic mucosal tissues of patients with PD and controls. Mucosal biopsy tissues of the ENS were obtained using standard biopsy forceps by endoscopic gastroduodenoscopy or colonoscopy. Two variants within the <I>SNCA</I> gene (the single nucleotide polymorphism [SNP] rs11931074 and the microsatellite REP1) were genotyped.</P> <P><B>Results</B></P> <P>In patients with PD, the rs11931074 (G allele) was significantly associated with the presence of α-Syn immunostaining in the ENS (OR = 5.96, 95% CI = 1.70–20.97, <I>P</I> = 0.01). In an interaction analysis, SNP rs11931074–PD status interaction was significantly associated with positive α-Syn immunostaining in the ENS (OR = 7.33, 95% CI = 1.58–33.88, <I>P</I> = 0.01). Longer <I>SNCA</I> REP1 alleles were not associated with positive α-Syn immunostaining in the ENS.</P> <P><B>Conclusion</B></P> <P>This exploratory study demonstrated that α-Syn deposition in the ENS may be associated with <I>SNCA</I> variants in patients with PD.</P> <P><B>Highlights</B></P> <P> <UL> <LI> Determining factors for alpha-synuclein (α-Syn) deposition in the enteric nervous system (ENS) of humans are still unclear. </LI> <LI> <I>SNCA</I> rs11931074 (G allele) is associated with α-Syn immunostaining in the ENS in patients with Parkinson's disease (PD). </LI> <LI> α-Syn deposition in the ENS may be associated with <I>SNCA</I> variants in patients with PD. </LI> </UL> </P>

Solubilization of Alcohols in Aqueous Solution of Cetylpyridinium Chloride

Chung Jong Jae,Lee Sang Wook,Kim Young Chul Korean Chemical Society 1992 Bulletin of the Korean Chemical Society Vol.13 No.6

The critical micelle concentration (CMC) values of cetylpyridinium chloride (CPC) in some alcohol-aqueous solutions were determined by UV-Vis spectroscopy at 25$25^{\circ}C$. The CMC of CPC was increased with the addition of methanol and ethanol, while with the addition of propanol it was decreased because of the solubilization of propanol into the micelle of CPC. The ratio (${\beta}$) of the number of counterions to that of surfactant ions associated into micelles in alcohol (methanol, ethanol and propanol) aqueous solutions was measured by using the Shinoda $equation^{17}$. The ratio of counterion binding to the CPC micelles in methanol-and ethanol-water mixtures was larger than in pure water, while the ratio in propanol-water mixture might be much decreased.

New Assessment of the N1-N2 Substaging in Stage Ⅲ Colorectal Cancer

Sung Wook Cho,Gyu Young Jeong,Ryung-Ah Lee,Kwang Ho Kim,Soon Sup Chung 대한외과학회 2010 Annals of Surgical Treatment and Research(ASRT) Vol.78 No.3

Purpose: The five-year survival rates of patients with stage Ⅲ colorectal cancer have been reported widely ranging from 22 to 69 percent. Hence, reliable substaging is important for the management of stage Ⅲ colorectal cancer patients. Therefore, we tried to assess the substages and investigate the possibility of other discriminating numbers for nodal substaging. Methods: The 381 patients with node-positive colorectal cancer who had undergone surgery, were retrospectively categorized by the number of positive nodes. The patients were grouped in five ways, and each grouping was divided into two subgroups according to the number of positive nodes. The subgroups of each grouping were as follows; in LN1 group, N1=1, N2>1; in LN2 group, N1=2, N2>2; in LN3 group, N1=3, N2>3; in LN4 group, N1=4, N2>4; in LN5 group, N1=5, N2>5. We compared the survival rate of each groups. Results: Node-positive patients had a five-year survival rate of 55.2 percent. The statistical differences between the N1 and N2 subgroups of each grouping were as follows: LN1 group (P=0.0128), LN2 group (P=0.0052), LN3 group (P=0.6268), LN4 group (P=0.1480), and LN5 group (P=0.6875). Conclusion: There were significant differences in the five-year survival rates between N1 and N2 in the LN1 group and LN2 group, but there were no differences between N1 and N2 in the other groupings. These data raise the possibility that a novel N1~N2 substaging (N1: 1~2; N2: >2) is superior to the current N1~N2 substaging (N1: 1~3; N2: >3).

Molecular Cloning and Characterization of a Large Subunit of Salmonella typhimurium Glutamate Synthase (GOGAT) Gene in Escherichia coli

Chung Tae-Wook,Lee Dong-Ick,Kim Dong-Soo,Jin Un-Ho,Park Chun,Kim Jong-Guk,Kim Min-Gon,Ha Sang-Do,Kim Keun-Sung,Lee Kyu-Ho,Kim Kwang-Yup,Chung Duck-Hwa,Kim Cheorl-Ho The Microbiological Society of Korea 2006 The journal of microbiology Vol.44 No.3

Two pathways of ammonium assimilation and glutamate biosynthesis have been identified in microorganisms. One pathway involves the NADP-linked glutamate dehydrogenase, which catalyzes the amination of 2-oxoglutarate to form glutamate. An alternative pathway involves the combined activities of glutamine synthetase, which aminates glutamate to form glutamine, and glutamate synthase, which transfers the amide group of glutamine to 2-oxoglutarate to yield two molecules of glutamate. We have cloned the large subunit of the glutamate synthase (GOGAT) from Salmonella typhimurium by screening the expression of GOGAT and complementing the gene in E. coli GOGAT large subunit-deficient mutants. Three positive clones (named pUC19C12, pUC19C13 and pUC19C15) contained identical Sau3AI fragments, as determined by restriction mapping and Southern hybridization, and expressed GOGAT efficiently and constitutively using its own promoter in the heterologous host. The coding region expressed in Escherichia coli was about 170 kDa on SDS-PAGE. This gene spans 4,732 bases, contains an open reading frame of 4,458 nucleotides, and encodes a mature protein of 1,486 amino acid residues (Mr =166,208). The EMN-binding domain of GOGAT contains 12 glycine residues, and the 3Fe-4S cluster has 3 cysteine residues. The comparison of the translated amino acid sequence of the Salmonella GOGAT with sequences from other bacteria such as Escherichia coli, Salmonella enterica, Shigella flexneri, Yersinia pestis, Vibrio vulnificus and Pseudomonas aeruginosa shows sequence identity between 87 and 95%.

Prognostic factors predicting survival rate over 10 years of patients with intrahepatic cholangiocarcinoma after hepatic resection

Chung Hyeun Ma,Dae Wook Hwang,Ki Byung Song,Song Cheol Kim,Sang Hyun Shin,Jae Hoon Lee 대한외과학회 2020 Annals of Surgical Treatment and Research(ASRT) Vol.98 No.3

Purpose: Hepatic resection is considered as the optimal treatment for intrahepatic cholangiocarcinoma (IHCC); however, the survival rate after resection is low and the analysis of long-term (≥10 years) survivors is rare. This study aims to analyze the clinicopathological factors affecting the long-term survival of patients with IHCC. Methods: Between January 2003 and December 2012, a single-institution cohort of 429 patients who underwent hepatic resection for IHCC were reviewed retrospectively. Surgical results, recurrence, and survival rates were investigated, and multivariate analyses were performed to identify prognostic factors. Results: The overall 1- , 3- , 5- and 10-year survival rates of patients were 76.5%, 44.1%, 33.3%, and 25.1%, respectively. Multivariate analysis showed that the serum CA 19-9 level (≥38 U/mL) (P < 0.001), lymph node (LN) metastasis (P = 0.001), and lymphovascular invasion (LVI) (P = 0.012) were independent factors associated with overall survival. In particular, CA 19-9 level and histologic type were determined to be independent factors affecting survival for more than 10 years. Conclusion: CA 19-9 (≥38 U/mL), LN metastasis, and LVI were identified as independent risk factors for survival after resection of IHCC. CA 19-9 (<38 U/mL) and histologic type were independent factors predicting survival for more than 10 years.

Clinical Survey of Patients in the Post-Anesthesia Care Unit

Sung Su Chung,Myung Gi No,Seong Wook Jeong,Sang Hyun Kwak,Woong Mo Im Korean Society of Critical Care Medicine 1999 Acute and Critical Care Vol.14 No.1

BACKGOUND: The purpose of this study was to evaluate characteristics and mortality rates of patients admitted to the post-anesthesia care unit (PACU) for obtaining the better clinical guidances and more advanced therapeutic plan in the future. METHODS: The medical records of total 425 patients admitted to the PACU from January to December 1998 were reviewed and analyzed according to age, sex, department, duration of stay, mechanical ventilator care, and mortality rates. RESULTS: Patients admitted PACU were 6% of total anesthesia patients. The ratio of male to female was 1.5:1. Patients of manhood aged from 45 to 64 were 37%, pediatric patients under 15 year-old were 23%. and elderly patients over 64 year-old were 18% of total PACU patients. The ratio of patients with ventilatory support was 42%. Mortality rate of neonate under 1 month of age was about 47%. Total mortality rate was 12%. CONCLUSIONS: To improve the outcome of the patients in PACU, continuous nutritional and medical support, cardiovascular and pulmonary monitoring, appropriate nursing care, and availability of medical staff were needed.

A Novel Waveguide Grating Router Based on Multimode Interference Couplers in InP

Sung Wook Moon,Young Chul Chung,Kwang Ryong Oh,Chan Yong Park 한국통신학회 1997 OPTOELECTRONICS & COMMUNICATIONS CONFERENCE Vol.2 No.1

Study of Cresol-Novolac Epoxy Systems on Fusion Bonded Epoxy Coatings for Pipeline Protection

Chung, Chi Wook,Lee, Sang Sun,Chai, Soo Gyum,Lim, Jong Chan 한국부식방식학회 2003 Corrosion Science and Technology Vol.2 No.4

Fusion Bonded Epoxy(FBE) systems have been widely used to protect pipelines for over 30 years. Numerous attempts have so far been made to improve the properties of FBE coatings such as chemical resistance, adhesion, water resistance, cathodic disbondment resistance, impact resistance, and flexibility to protect pipelines at a wet and a high temperature condition. But these attempts have not been successful in reducing some weakness, for instance, in pipeline operating at high temperature due to poor hot water resistance and cathodic protection. The purpose here is to build a basis for getting better corrosion resistance of FBE systems. Cresol-novolac epoxy coating systems were studied compared to bisphenol A type epoxy systems. After the immersion of the film in water at a high temperature for a long period, good adhesion to metal substrate and excellent cathodic disbond resistance were observed in the cresol-novolac epoxy resin systems. It is well known that the adhesion of organic coatings to metal substrate might be decreased due to the disruption of a chemical bond across the film and metal interface induced by water molecules. A high crosslinking density might decrease water permeability and Improve cathodic disbonding protection in the coatings. Other factors are studied to understand anti-corrosion mechanism of Cresol-novolac epoxy coatings. In addition, the water absorption rate and the effect of cure temperature on the adhesion and cathodic disbonding resistance of the films were studied in different epoxy coatings and the effect of substrate was evaluated. The results of field application are proved that the Cresol-novolac epoxy coating system developed recently is one of the most suitable coatings for protection of pipelines.