Aspergillus parasiticus R-716의 生育 및 Aflatoxin生産에 미치는 薑黃(curcuma longa L.) 엑기스의 影響

鄭德和 慶尙大學校 1984 論文集 Vol.23 No.2

동양에서 오랫동안 암과 비슷한 질병의 치료에 사용되어 온 薑黃(Curcuma longa L.)이 供試菌(Aspergillus parasiticus R-716)의 生育 및 aflatoxin 生成에 미치는 영향을 조사하기 위하여 물이나 유기용매로서 엑기스를 조제하였다. 薑黃은 chloroform에 의한 추출이 가장 果的이었고 첨가하는 엑기스농도의 증가에 따라 供試菌의 生育과 aflatoxin 生育은 抑制되었고, 薑黃엑기스를 0.2ml 첨가하였을 경우 대조구는 배양 3일후 약 80%의 균체증식을 보였으나 첨가구는 4일부터 형성이 시작되어 9일째에는 0.474g/25ml로 52%가 저해되었고, aflatoxin도 대조구는 5일후에 대체로 생성되었으나, 첨가구는 늦어져 9일째는 대조구의 69%인 946㎍/25ml로서 감소되었다. 그러나 균체 g당 aflatoxin 함량과 배양기간 중 NADPH oxidase 활성은 첨가구가 오히려 높았다. The possible effects of Ganghwang(Curcuma longa L.), which have long been used to treat cancer like disease in oriental countries, on growth and aflatoxin production by Aspergillus parasiticus R 716 were investigated. The most effective solvent used for extraction of Ganghwang was chloroform. The growth and aflatoxin production decreased with the increase in extract concentration. With the addition of 0.2ml Gangwhang extract in 25ml SLS medium, the inhibition of mold growth was delayed for about 4 days and after 9 days, mycelium weight was inhibited 52%(0.474g/25ml) of that showed in the control(0.985g/25ml). and total aflatoxin was also reduced 31%(946㎍/25ml) of that produced in the control(1352㎍/25ml). but aflatoxin per mycelium weight and activity of NADPH oxidase were higher as compared to the control.

Mycotoxin을 중심으로 한 전통식품의 위생학적 연구

정덕화 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.1

Certain Fungi including Aspergillus flavus produce low molecular secondary metabolite that is toxic to human and animals, which have been termed mycotoxin. Given the proper humidity and temperature like summer in Korea, are capable of growing of those hazard fungi and elaborating mycotoxin on almost any organic substrate such as traditional foodstuffs and their raw materials including rice, barley, corn, meju, doenjang and gochujang etc. Until now, some people have examined to isolate various fungi such as Aspergillus sp., Penicillimn sp, and Fusarium sp. from traditional foodstuffs and raw materials, and have screened various mycotoxin producing strains. Some mycotoxin contamination such as aflatoxin, ochratoxin, deoxynivalenol(DON) and zearalenone etc. also have been confirmed from similar above samples. But these data are different each other and inconsistent in experimental conditions and methods. Especially, almost experiments have been finished for one time. So more consistent experimental method and data are necessary to evaluate objectively the safety of traditional foodstuffs subjected to the mycotoxin. For this purpose, we have to apply a new advanced technology to develop more simple and rapid methods for determination of mycotoxin and also have to concentrate our efforts on activation of research and accumulation of technology with sustaining investment of financial support and enlargement of research installation. With those harmonious efforts, it should be possible to examine continuously and systematically the mycotoxin contamination in our traditional foodstuffs and to assure the safety of them. Then we can maintain and develop the better traditional foodstuffs suited to internationalization.

진주지역 아동 복지 시설에서의 피복환경에 대한 미생물 평가

정정숙,박선자,정덕화 대한보건협회 2002 대한보건연구 Vol.28 No.4

This study was conducted to assess the microbial level of cloth environment at five child welfare institutions(A, B, C, D, E) in Jinju District. Four major categories for experiment were personal hygiene, cloth hygiene of chilfren, cloth environment in indoor and kitchen which were involved thirteen minor items. Main assessment tool on this research was total bacteria count. Whereas total bacteria count at B child welfare institution was high as 1.96× 10^4CFU /100cm^2 in hands, hair hygiene was good at the same place. According to cloth hygiene of children, hygienic condition of E place was bad with a high level of bacteria counts from various items including socks, shoes. Total bacteria of indoor cloth environmemt at all child welfare institutions was beyond the standatd level, but indoor air was more clean than other items. In cloth environment of kitchen, dish cloth and apron of C place showed high level of total bacteria counts with 1.72×10^3 CFU/100cm^2 and TNTC, respectively. fungi were also screened from every items except hair, and detected as high frequency in socks of A, B, C and E places. Among them, B place was oftenly contaminated with fungi in various items such as socks, bedcloth, apron and rubber gloves. In conclusion, periodic surveillance on microbial assessment of cloth environment should be necessary for the safety of cloth environment at child welfare institutions, so more hygienic practice and education of staff members were also reinforced.

T-2 Toxin이 병아리 비장세포의 유전질 발생에 미치는 영향

전향숙,정덕화,이서래 이화여자대학교 생명과학연구소 1994 생명과학연구논문집 Vol.5 No.-

시험관 내에서 T-2 toxin이 병아리 비장세포의 blastogenesis에 미치는 영향을 살펴본 결과, B-cell mitogen인 lipopolysaccharide및 T-cell mitogen인 concanavalin A자극에 대해 T-2 toxin의 농도가 증가함에 따라 억제정도가 증가하는 경향을 나타내었다. 노출시기를 달리하여 T-2 toxin을 투여한 병아리 비장세포에서 mitogen 자극에 대한 반응을 알아 본 결과, 부화하기 전과 후에 계속 T-2 toxin에 노출시킨 실험군은 가장 영향을 많이 받은 것으로 나타났고 부화전 혹은 부화후 어느 한 시기에만 T-2 toxin에 노출된 실험군은 비교적 영향을 적게 받는 것으로 나타났다. The effects of T-2 toxin of mitogen-induced blastogenesis of chick splenic cells were investigated. The[^3H]thymidine incorporation in splenic cells stimulated by lipopolysaccharide and concanavalin A were equally inhibited as the concentration of T-2 toxin was increased. The effective dose of T-2 toxin causing a 50% reduction of[^3H]thymidine incorporation was inbetween 1.0 and 5.0 ng/㎖ for both mitogens. Mitogen-induced blastogenesis in chick splenic cells showed differences among experimental groups with different exposure time of T-2 toxin, exhibiting the most inhibition in the experimental group exposed to T-2 toxin at both embryonic and chick periods.

다단계 검출알고리즘을 이용한 웨이블렛기반 이미지 워터마킹

이구영,정용덕,이종원,김정화 조선대학교 전자정보통신연구소 2002 電子情報通信硏究所論文誌 Vol.5 No.2

In this paper, we propose a watermarking algorithm of multiresolution watermarking based on wavelet transform for digital images. Watermark insertion is add to pseudo random sequences to the wavelet coefficients at the high and middle frequency band of the discrete wavelet transform. Instead of previous method, proposed multiresolution watermark decoder that allows efficient identification of the embedded a bank of watermarking key without prior notice of it. Proposed detection method aims at ensuring the maximum exactitude in the detection of the owner identification key and, minimizing the number of false positive detection.

한약재가 Aspergillus parasiticus R-716의 生育과 aflatoxin 生成에 미치는 影響

구성회,이용욱,정덕화,김종규 한국식품위생학회 1988 식품위생학회지 Vol.3 No.2

한의원에서 오래 前부터 사용되어 오고 있는 몇 종의 한약재를 구입하여 이들을 chloroform으로 추출하여 추출물을 조제한 다음 공시균(Aspergillus parasiticus R-716)의 生育과 aflatoxin 生成에 미치는 影響을 조사한 결과 다음과 같은 結論을 얻었다. 1. 목단 추출물 첨가군에서 가장 현저하게 공시균의 生育이 저해되었고 그외의 첨가군에서도 강황, 봉출, 향부자, 백작액, 백출의 順으로 저해되었다. 2. Aflatoxin 生成은 백출과 강황 추출물 첨가군에서만 저해되었고, 나머지 첨가군에서는 오히려 증가되거나 효과가 적었다. 3. 백출 추출물 0.2ml첨가시 3일째부터 菌體 生成을 시작하여 9일째에 0.953g/30ml이었고, aflatoxin 함량은 792㎍으로서 대조군에 비해 약 50%가 감소되었다. 菌體 g當 aflatoxin 함량은 992㎍으로서 대조군의 1,467㎍에 비해 역시 크게 감소되었으며 NADPH oxidase 活性은 대조군보다 높아 백출 추출물이 공시균의 生育과 aflatoxin 生成에 저해 효과가 있는 것으로 나타났다. The possible effects of some oriental herbs, which have been used to treat cancerlike disease in Korea, on growth and aflatoxin production by Aspergillus parasiticus R-716 were investigated. Zedoaria rhizoma, Curcuma longa, Cyperus rotundus, Angelica gigas, Paeonia albiflora, Paeonia mountan, Atractylis ovata, and Pulsatilla koreana were extracted with chloroform. Among them the extract of Paeonia mountan was remarkably effective on the growth inhibition, and Curcuma longa, Zedoaria rhizoma, Cyperus rotundus, Paeonia albiflora, Atractylis ovata also inhibited the growth. The extract of Atractylis ovata and Curcuma longa, also inhibited the aflatoxin production but the others showed no effect at all or sometimes stimulated effect. With the addition of 0.2ml extract of Atractylis ovata in 30ml SLS medium, the growth was delayed for about 2 days, and after 9 days, mycelium weight was 0.953g, and total aflatoxin was reduced 50%(792㎍)of that produced in the control(1547㎍). Aflatoxin per mycelium weight was decreased 32%(992㎍)of that produced in the control(1467㎍), but NADPH oxidase was higher as compared to the control. The extract of Atractylis ovata appeared to have a inhibitory effect on the growth and aflatoxin production of Aspergillus parasiticus R-716.

Aflatoxin-DNA Adduct의 화학합성에 관한 연구

최상경,김성영,강진순,정덕화 경상대학교 유전공학연구소 1993 遺傳工學硏究所報 Vol.12 No.-

Aflatoxin은 세계 여러 곳에서 식품에 오염되어 발견되며 증가하는 인간의 간암발생과 빈번히 역학적으로 관련하여 보고되는 발암물질이다. 이러한 aflatoxin의 발암성 규명에 대한 연구의 일환으로 20㎎ calf thymus DNA와 8㎎ 표준 aflatoxin B₁을 반응시켜 화학적으로 aflatoxin B₁-DNA adduct를 합성하였다. 합성된 aflatoxin B₁-DNA adduct는 산가수분해와 열처리에 의해 거대한 DNA 분자를 절단하였고, immunoaffinity column을 통과시켜 aflatoxin B₁-DNA adduct만 선택적으로 정제한 후 HPLC법으로 정량하였다. 그 결과 반응생성물의 대부분은 aflatoxin B₁-guanine adduct였으며, 그 함량은 aflatoxin B₁으로 5.2㎎이었다. Aflatoxins are highly carcinogenic agents consistantly found as contaminants in human food supplies in many areas of the world and epidemiologically linked to incidence of human liver cancer. To examine the carcinogenic action of aflatoxin B₁,aflatoxin B₁-DNA adducts were chemically synhtesized with the reaction of 20㎎ calf thymus DNA, and 8㎎ standard aflatoxin B₁. Since DNA molecule was too large for analysis, it was fragmented by acid hydrolysis and heat. The fragmented aflatoxin B₁-DNA adducts were selectively concentrated by immunoaffinity column procedure and confirmed by HPLC method. The main component was aflatoxin B₁-guanine adduct, which was quantatively measured as 5.2㎎ aflatoxin B₁.

토끼의 Aflatoxin B₁중독증에 대한 인삼엑기스의 효과 : 임상, 생리적 및 병리학적 변화 Clinical, Physiological and Pathological Changes

김차용,변유성,문평일,정덕화,김종수 慶尙大學校 1987 論文集 Vol.26 No.1

Chinchilla 계통 1~1.5㎏ 되는 토끼 30마리를 5군 으로 나누어 체중㎏당 제 1군은 대조군으로서 Cornoil 0.3ml 씩 제 2군은 aflatoxin B? 0.03mg과 인삼 엑기스 8mg을 제 5군은 aflatoxin B? 0.06mg과 인삼엑기스 8mg을 3주동안 카테타로 경구 투여하여 임상, 혈액학적 및 병리학적 변화를 관찰하였다. 1.사료소비량 감소, 침울, 쇠약, 혼수, 체중증가율감소등과 같은 임상증상은 대조군을 제외한 전 처리군에서 독을 투여후 3일째 부터 전처리기간을 통하여 나타났으나 aflatoxin B? 단독처리군에서 인삼엑기스 혼합처리군보다 더 현저하게 나타났다. 2. 혈청 효소와 AST, ALT는 독물투여후 7일부터 인삼엑기스 혼합투여군 보다 aflatoxin B? 단독 투여군에서 높게 증가하였다. 3.전처리군에서 적혈수용적, 헤모글로빈농도는 영향을 받지 않았고, 혈장단백질은ㅇ 독물투여후 14일이후 aflatoxin B?단독 처리군에서 현저하게 감소하였다. 4. Aflatoxin B?과 인삼엑기스 혼합 투여군 보다 aflatoxin B? 단독투여군에서 간장의 공포변성,담관증생, 핵농축, 붕괴, hepatic fibrosis와 임파절 및 비장에서 현저한 임파양 세포의 소실을 나타내었다. The present study has been carried out to investigate the effectsof ginseng extract on induced chronic aflatoxicosis in rabits. Groups of rabbits were treated orally with aflatoxin B? and mixture of ginseng extract for 21 days with a dosage of 0.03mg, 0.06mg of the toxin and 0.03mg or o.o6mg of the toxin mixture of 8mg of ginseng extract or 0.3ml corn oil s place to per kg body weight. The results obtained were is follows. 1. Sings o toxicosis included reduction of feed consumption emaciation, decrese of body weight and coma revealed in both groups treated with 0.03mg pr 0.06,mg of aflatoxin B?/Kg/B.W than groups 4.5 after days 3. 2. In groups 2, 3(aflatoxin B? 0.03, 0.06mg), Activity of asparate aminotransferase(AST) and alanine aminotransferase (ALT)were increased sigificantly in dats7, 14 and 21. 3. In all groups, Mean values for packed cell volume(PCV)and hemogobin were unremarkasble, but plasma total protein were decreased in groups 2,3than 4,5 groups on days 14 and 21. 4. The rabits treated with aflatoxin B?(2,3 groups)showed markedly degenerative chasnges ranging from swelling and decreaseed granularity to marked vacuolation, hepatic necrosis, fibrosis and bile duct, Proliferation and depletion of lymphocytes in lymphocytes in lymphonde, spleen than groups 4,5.

Allylisothiocyanate 첨가가 Aflatoxin 생성 곰팡이 대사산물의 생합성에 미치는 영향

강성조,여명재,이은일,송재영,정덕화 동아시아식생활학회 1996 동아시아식생활학회지 Vol.6 No.1

The effects of allylisothiocyanate on the biosynthesis of various fungus metabolites such as sterigrnatocystin, lipid, protein, citrate RNA and AMP from the culture of Aspergillius parasiticus R-716 were investigated. The content of sterigmatocystin, the precursor of aflatoxin, was lower in the culture added with 50ppm allylisothiocyanate after 48 hours, however was rather higher after 144 hours compared to that of the control. The addition of allylisothiocyanate resulted in the increase of lipid, protein, RNA in mycelium and the content of citrate in the media, but the amount of AMP was low.

The Effects of Various Vegetables on Growth Characteristics and Aflatoxin Production of Aspergillus Parasiticus R - 716

( Duck Hwa Chung ) 대한보건협회 1985 대한보건연구 Vol.11 No.2