Al₂O₃ TiO₂ 담체종류별 Pt, PtRu 촉매의 산화특성

김문찬,신진실,이동구,최균 한국공업화학회 2005 응용화학 Vol.9 No.1

The volatile organic compounds (VOCs) have been recognized as major contributor to air pollution. The catalytic oxidation is one of the most important processes for VOCs destruction clue to getting high efficiency at low temperature. In this study, mono metallic Pt and bimetallic Pt-Ru were supported to TiO₂ and γ-Al₂O₃. In order to distribute metals uniformly, Xylene, toluene and MEK were used as reactants. The mono metallic or bimetallic catalysts were prepared by the excess wetness impregnation method and were characterized by XRD, XPS, TEM and BET analysis. As a result, Pt-Ru bimetallic catalysts were showed higher conversion than Pt mono metallic catalyst. Pt-Ru bimetallic catalyst was showed highest conversion on the γ-Al₂O₃support. In the VOCs oxidation, Pt-Ru bimetallic catalyst had multi point active sites, so it improved the range of Pt metal state. Therefore, bimetallic catalysts were higher conversion of VOCs than mono metallic ones.

혈관평활근세포에서 산화에너지대사 억제에 의한 아밀로이드전구단백질 대사의 변화

한문구,최웅,김헌식,안희열,한설희 대한치매학회 2002 Dementia and Neurocognitive Disorders Vol.1 No.1

Background: A reduction in the activity of cytochrome c oxidase(COX) has been recently identified in mitochondria from platelets and postmortem brain tissue of AD patients Sodium azide (NaN₃). a COX inhibitor, is an effective chemical agent producing energy shortage and oxidative stress both in vitro and in vivo system Furthermore it has been suggested that vascular compromise could be either directly involved AD pathogenesis or indirectly associated with triggering pathogenetic events leading to AD This study was performed to investigate amyloid precursor protein (APP) metabolism by inhibition of mitochondrial energy metabolism in cultured vascular smooth muslce cells (VSMCs) Materials and Methods: VSMCs isolated from the aorta of seven weeks old Spraque-Dawley rat were treated with NaN₃in a low concentration (100-500μM) or in a high concentration (1-100mM) Cellular proliferation and viability were determined by MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenly)-2H-tetrazolium) assay Cellular APP was detected with N-terminal specific antibody 22C11. Celldeath was determined by observation of morphology and terminal deoxynucleotidyl transferase-mediated dUTP-fluorescein nick end labeling stain (TUNEL) We used ginkgo biloba extract(EGb761) and melatonin as anti-oxidants to investigate the mechanism of latered APP metabolism Results: The viability of VSMCs was increased after treatment with 1 mM and 10mM NaN₃(p<0.05) unitl 3 hr and then dimnished Many TUNEL positive cells were found in 10mM and 100mM treatment group. but were not apoptotic in nature 22C11 immunoreactivity was not changed at 3 hr, 6hr, 12hr Anti-oxidants reduced cellular proliferation (p<0.05). but did not block TUNEL positivities and did not influence the 22C11 immunoreactivity In a low concentration NaN₃ treatment group the viability of VSMCs was increased concentration dependently(p<0.05) Immunoblot with 22C11 showed the concentration dependent decrease at 145 kDa, 125 kDa. and high molecular weight range (>160kDa) TUNEL staining showed DNA fragmentations and condensations of nuclear chromatin suggesting apoptosis After treatment with anti-oxidants, the cellular proliferation was more decreased (p<0.05), and TUNEL positive cell deaths were blocked Immunoreactivities of 125 kDa (immature APP). 145 kDa (mature APP). and higher molecular weight bands were recovered below 400μM of NaN₃ Immunoreactivity of 145 kDa was recovered in 100 μM NaN₃ treated group Conclusions: The presumed mechanism of low concentration COX inhibitor is the overproduction of reactive oxygen species resulting from a depression of the mitochondiral electron transport chain. whereas potential consequence of high concentration COX inhibitor might be related to decreassion of ATP synthesis and bioenergetic impairment Reactive oxygen radicals in response to low concentration COX inhibitor alter the processing of APP in VSMCs This investigation demonstrated analtered APP metabolism as a peripheral marker of AD Therefore VSMCs treated with low concentration COX inhibitor could be concsidered as a novel in vitro model of AD.

초등 교육 발전에 기여하는 교육대학의 현장지도 역할에 관한 연구

김성회 / 이종철 / 전구호 / 정용팔 / 최동준 / 문낙주 공주교육대학교 초등연구원 1977 公州敎大論叢 Vol.13 No.2

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Efeect of Necropsy Days on Number of Clonorchis sinensis and Plaque Forming Cells after Challenge in Hamsters

Moon, Han Ku,Moon, Chu Hwan,Kong, Hyun Hee,Choi, Dong Wik 慶北大學校 醫科大學 1994 慶北醫大誌 Vol.35 No.2

Challenge 感染된 햄스터에서 屠殺日이 肝吸蟲과 PFC의 數에 미치는 影響을 決定하기 위해 肝吸蟲 成蟲의 代謝産物과 Freund's incomplete adjuvant로 感作된 golden 햄스터의 腹腔渗出細胞와 血淸을 recipient 햄스터의 腹腔內에 注入하였다. 第1次 感作한지 7日 後에 肝吸蟲 被囊幼蟲 20個씩 經口感染시킨 다음, 50日, 70日, 90日 그리고 110日에 各各 屠殺하였다. Challenge感染 後, EpG는 對照群에서 感作群보다 하루 일찍 나타났고, 對照群과 感作群 모두 漸次로 增加되다가 40日에서 65日사이에 最高値를 나타낸 후 漸次 減少하였다. 그러나, 感作群 사이에 EpG의 有意的 差는 認定할 수가 없었다. 蟲體回收率은 感作群과 對照群 사이에 有意的 差를 認定할 수 있었다. 蟲體回收率은 Challenge感染후, 약 70日 前後에 減少되었다. Plaque 形成細胞는 感作後 50日과 70日의 感作群에서 檢出되었으나, 그 以後의 感作群 및 對照群에서는 檢出되지 않았다. 以上의 成績으로 미루어 보아 golden 햄스터의 適切한 屠殺日은 Challenge感染 後 50日에서 70日이 適當함을 알 수 있었다. OBJECTIVE, MATERIALS AND METHODS: To determine the appropriate necropsy day in the passive transfer of immunity against Clonorchis sinensis in golden hamsters, the donor hamsters were sensitized with two injections of the admixture of the metabolic products of C. sinensis and Freund's incomplete adjuvant into footpads at 2 weeks' intervals. The peritoneal cells and sera of sensitized and control donor hamsters were injected intraperitoneally to the recipient hamsters, seven days after primary sensitiza-tion. Four groups of the recipient and control hamsters were challenged with 20 metacercariae of C. sinensis, and killed on the 50th, 70th, 90th and 110th day after challenge infection. RESULTS: The eggs of control hamsters appeared one day earlier than those of the recipient groups, and the eggs per gram of feces in both recipient and control groups increased stepwise and continued at high levels from the 40th to 65th day and then decreased irregularily after challenge infection. There was no significant difference in EpG of feces between the sensitized recipient and control groups, but there was significant difference in the worm burden of C. sinensis between the recipient and control groups. The recovery number of the fluke in the recipient and control hamsters decreased about the 70th day after challenge infection. CONCLUSIONS: These results indicate in part that the appropriate necropsy day of golden hamsters would be estimated around the 50th day after challenge infection of C. sinensis.

Hydroa Vacciniforme - Recurrence at Adulthood and Confirmative Diagnosis by Repetitive Ultraviolet-A Phototesting -

( Joo Hyun Choi ),( Seung Kyung Hann ),( Moon Soo Yoon ),( Byung Moon Choi ),( Sung Ku Ahn ),( Yoon Kee Park ) 대한피부과학회 1989 Annals of Dermatology Vol.1 No.2

Evaluation of QTL Related SSR Marker Universality in Korean Rice Breeding Populations

Moon-Tae Song,Jeom-Ho Lee,Sang-Bok Lee,Ja-Hwan Ku,Youn-Sang Cho,Myung-Hee Song,Sung-Ho Choi,Hung-Goo Hwang 韓國作物學會 2003 Korean journal of crop science Vol.48 No.1

If a quantitative trait loci (QTL) marker identified in a population is applicable to different populations (marker universality), this will not only reduce the labor and cost in marker assisted selection (MAS), but accelerate the application of molecular markers to real breeding programs. Present study aims to evaluate the defined QTL related markers from a population to a different breeding population for the MAS. Four rice breeding populations were subjected to seventy-five simple sequence repeat (SSR) markers which were already identified for their polymorphism information content (PIC) in the parents of the crossings. Among them, eight markers were evaluated for their correlation between presence of marker alleles and phenotypic expression in breeding populations. A reasonable level of polymorphism for the mapped markers originated from any sources of rice accessions was observed between crosses of any sources (marker repeatability). However, correlation between presence of markers and expression of the traits in rice breeding populations was not significant except for minor portion of traits and markers examined (failure of marker universality). In the present study, various strategies were discussed to develop new markers with universality of breeding application.

실리카 도파로형 WDM 광 필터의 설계 및 해석에 관한 연구

최대필,문성욱,정영철,신장욱,정명영,최태구 광운대학교 신기술연구소 1997 신기술연구소논문집 Vol.26 No.-

본 논문에서는 Mach-Zehnder 간섭기로 구성된 1×4 필터와 성형결합기로 구성된 5×5 광도파로 격자 라우터를 BPM CAD TOOL을 이용하여 설계하였고 최적화 하였다. 마스크 레이아웃을 효율적으로 실현하기 위한 전체 도파구조를 설계하였다. BPM 전산모사를 통해 설계된 소자의 WDM 특성을 0.8 nm(100 ㎓)와 8.0 nm(1000 ㎓)의 채널간격에 대해서 살펴보았다. 설계된 소자는 두가지 채널간격 모두에 대하여 3.5dB 이내의 잉여손실, 0.5dB 이내의 균일성과 -22dB 이내의 누화(crosstalk)특성을 보임을 확인하였다. In this paper, we designed and optimized 1×4 WDM Filter composed of Mach-Zehnder Interferometer and 5×5 Waveguide Grating Router(WGR) composed of two star couplers. The overall guiding structure is designed to realize the mask layout efficiently. Through the BPM simulation, we demonstrated WDM characteristics of these devices with 0.8 nm and 8.0 nm channel-spacing. It is shown that the excess loss is less than 3.5dB, uniformity less than 0.5dB and the crosstalk smaller than –22 dB for both the channel spacings.

의료보험수가 적정조정률 산정에 관한 연구

문옥륜,장동민,이석구,강선희,박실비아,최상은 서울大學校保健大學院 1995 國民保健硏究所硏究論叢 Vol.5 No.2

Since the introduction of the National Health Insurance Program as of 1977, the charges for medical treatment have been increased as much as by 15 times. In general, Fee schedules should be decided upon by taking account of the ability to pay for medical services by consumers, and the financial status of both insurers and medical facilities. As the policy makers have mainly concerned about controlling the price of medical service, the level of fee schedules has kept low: the accumulative index of the charge for medical treatment showed only 89% as against the accumulative consumer's price index during the last 18 years. This lower increasing rate has caused us many undesirable outcomes. For example, medical practices have been twisted to orient toward higher earning practices and high cost equipments have been introduced imprudently. This study is to develop a new method of determining the adequate increasing rate for medical fee schedules. The data complied by KIHM were used such as 'Analysis of the Hospital Management, 1992' and the 'study on the Adjusted Increasing Rate for the Insurance Fee Schedules, 1994'. The basic model of updating fee schedules have been developed to include deficit rates and the incentive for medical service quality improvement, and the final adjustment rate was derived from it accordingly. If the authorities decide to increase fee schedules as of January 1995, the adjustment by 12.59-14.63% will be enough and if it becomes June 1995, the rates will go up as much as by 21.58-25.08%. Furthermore, this study shows that the government should regularize the period of increasing time and this will make medical facilities to predict their operational budgets possible.

Antigenicity of HM10760 in Guinea Pigs

Choi, Woo-Hyuck,Lee, Sung-Hak,Lee, Mi-Chael,Chung, Moon-Ku,Kwon, Se-Chang,Lee, Gwan-Sun,Han, Jae-Yong,Koh, Woo-Suk Korean Society of ToxicologyKorea Environmental Mu 2005 Toxicological Research Vol.21 No.3

HM10760 is a recombinant human erythropoietin that has beer developed as a drug for anemia. In this study, antigenic potential of HM10760 was examined by active systemic anaphylaxis in guinea pigs and passive cutaneous anaphylaxis in a guinea pig-guinea pig system. HM10760 was subcutaneously administered at 0, 2, and $20{\mu}g/kg$ and also as a suspension with adjuvant ($20{\mu}g/kg$ + FCA). Ovalbumin as a suspension with adjuvant was administered to induce positive control responses. In the active systemic anaphylaxis test, no symptoms except rubbing or licking nose and urination that were considered as physiological phenomena were observed at $0{\mu}g/kg$. Four of 5 animals at $2{\mu}g/kg$ and all the 5 animals at $20{\mu}g/kg$ showed cyanosis and lying on side. All animals in the adjuvant mixture group showed relatively mild symptoms such as rubbing or licking nose, urination, and evacuation. In the passive cutaneous anaphylaxis test, 0/5, 3/5, and 5/5 serum samples from the animals immunized with 0, 2, and $20{\mu}g/kg$, respectively, showed positive reactions against HM10760. All 5 sera collected from the animals immunized with an adjuvant mixture contained HM10760-specific antibodies. These results suggest HM10760 have antigenicity in guinea pigs.

Impact of White Matter Changes on Activities of Daily Living in Mild to Moderate Dementia

Moon, So Young,Na, Duk L.,Seo, Sang Won,Lee, Jun-Young,Ku, Bon D.,Kim, Seong Yoon,Park, Kyung Won,Shim, Yong S.,Youn, Young Chul,Chung, Chan-Seung,Cheong, Hae-Kwan,Choi, Seong Hye,Cha, Kyung Ryeol,Kim S. Karger AG 2011 European neurology Vol.65 No.4

<P>The association between white matter changes and activities of daily living (ADL) in a large, well-defined cohort of patients with mild-to-moderate dementia (either Alzheimer’s disease or subcortical vascular dementia) were investigated. A total of 289 patients were divided into three groups (140 mild, 99 moderate, and 50 severe) depending on the degree of white matter changes as indicated on brain magnetic resonance image scans. Further, we analyzed the three groups’ performances on basic and instrumental ADL. The degree of white matter changes was associated with greater age, hypertension, previous history of stroke, higher Hachinski Ischemic Score, worse global cognitive and functional status, and an increased impairment of basic ADL and instrumental ADL. The increased impairment with regard to the severe group’s performance on both the basic and instrumental ADL remained significant after adjustment for age and hypertension. Tasks involving physical activities were most significant. This was the first study investigating the association between white matter changes and ADL in a large, well-defined dementia cohort. The present study suggests that severe white matter changes may be associated with higher impairment on both basic and instrumental ADL.</P><P>Copyright © 2010 S. Karger AG, Basel</P>