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Cooling Field Tests of Lychees (Litchi chinensis Sonn) Using Air Cooler and Hydrocooler Prototypes
Sergio Chatelain-Mercado,Dagoberto Castillo-Animas,Janeth Karina Jacuinde-Guzman,Fernando Rivera-Cabrera,Fernando Diaz de Leon-Sanchez,Laura Josefina Perez-Flores,Clara Pelayo-Zaldivar 한국원예학회 2006 한국원예학회 기타간행물 Vol.- No.-
주산기 태아 흰쥐의 뇌하수체 전엽에서 분비되는 ACTH의 다형현상
김희승(Kim, Hee-Seung),Chatelain, Alain 대한생리학회 1985 대한생리학회지 Vol.19 No.2
흰쥐의 태아에서 ACTH의 분비양상을 알아보기 위하여, 태아의 제태일수에 따라 혈장 및 뇌하수체 전엽에서 여러 분자형태의 ACTH를 방사면역측정법으로 측정하였다. 태아의 혈장 ACTH농도는 제태 19일에서 가장 높았으며 그후 계속 감소하여 출생후 1주에서 가장 낮은 값을 보였다. 출생 1주후부터 ACTH농도는 다시 증가하기 시작하여 출생후 21일에서는 거의 성체의 값에 도달하였다. 측정된 ACTH는 chromatogram상에서 항상 3가지 peak가 나타났다. 즉 big 형 ( big ACTH, MW ≈ 44,000), intermediate 형 ( intermediate ACTH, MW ≈ 13,000)및 little 형 ( little ACTH, MW ≈ 4,500)으로 구분되었다. 임신말기 (제태기간 17일에서 21일 사이)에서 태아 혈장의 ACTH는 little 형의 비율이 증가한 반면 big 형의 비율은 감소하였다. 그러나 뇌하수체 전엽에서 분비된 ACTH는 3가지 형이 같은 비율이었다. 뇌하수체 전엽에서 분리한 big 형의 ACTH를 시침관내에서 trypsin을 처리한 결과 intermediate 형과 little 형이 출현하였다. 이 결과로 미루어 태아 흰쥐의 뇌하수체에서 분비된 ACTH가 순환도증 다른 형으로 전환될 수 있음이 시사된다.
Polymorphism of ACTH Released by Adenohypophysis of Fetal Rat during Perinatal Period
김희승,Kim, Hee-Seung,Chatelain, Alain The Korean Physiological Society 1985 대한생리학회지 Vol.19 No.2
흰쥐의 태아에서 ACTH의 분비양상을 알아보기 위하여, 태아의 제태일수에 따라 혈장 및 뇌하수체 전엽에서 여러 분자형태의 ACTH를 방사면역측정법으로 측정하였다. 태아의 혈장 ACTH농도는 제태 19일에서 가장 높았으며 그후 계속 감소하여 출생후 1주에서 가장 낮은 값을 보였다. 출생 1주후부터 ACTH농도는 다시 증가하기 시작하여 출생후 21일에서는 거의 성체의 값에 도달하였다. 측정된 ACTH는 chromatogram상에서 항상 3가지 peak가 나타났다. 즉 'big'형 ('big' ACTH, $MW\approx44,000$), 'intermediate'형 ('intermediate' ACTH, $MW\approx13,000$)및 'little'형 ('little' ACTH, $MW\approx4,500$)으로 구분되었다. 임신말기 (제태기간 17일에서 21일 사이)에서 태아 혈장의 ACTH는 'little'형의 비율이 증가한 반면 'big'형의 비율은 감소하였다. 그러나 뇌하수체 전엽에서 분비된 ACTH는 3가지 형이 같은 비율이었다. 뇌하수체 전엽에서 분리한 'big'형의 ACTH를 시침관내에서 trypsin을 처리한 결과 'intermediate'형과 little'형이 출현하였다. 이 결과로 미루어 태아 흰쥐의 뇌하수체에서 분비된 ACTH가 순환도증 다른 형으로 전환될 수 있음이 시사된다.
Modal analysis and ambient vibration measurements on Mila-Algeria cable stayed bridge
Kibboua, Abderrahmane,Farsi, Mohamed Naboussi,Chatelain, Jean-Luc,Guillier, Bertrand,Bechtoula, Hakim,Mehani, Youcef Techno-Press 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.29 No.2
The seismic response analysis of an existing bridge needs a mathematical model that can be calibrated with measured dynamic characteristics. These characteristics are the periods and the associated mode shapes of vibration and the modal damping coefficients. This paper deals with the measurements and the interpretation of the results of ambient vibration tests done on a newly erected cable stayed bridge across the Oued Dib River at Mila city in Algeria. The signal analysis of ambient vibration records will permit to determine the dynamic characteristics of the bridge. On the other hand, a 3-D model of the bridge is developed in order to assess the frequencies and the associated modes of vibration. This information will be necessary in the planning of the test on the site (locations of the sensors, frequencies to be measured and the associated mode shapes of vibration). The frequencies predicted by the finite element model are compared with those measured during full-scale ambient vibration measurements of the bridge. In the same way, the modal damping coefficients obtained by the random decrement method are compared to those of similar bridges.
Modal analysis and ambient vibration measurements on Mila-Algeria cable stayed bridge
Abderrahmane Kibboua,Mohamed Naboussi Farsi,Jean-Luc Chatelain,Bertrand Guillier,Hakim Bechtoula,Youcef Mehani 국제구조공학회 2008 Structural Engineering and Mechanics, An Int'l Jou Vol.29 No.2
The seismic response analysis of an existing bridge needs a mathematical model that can be calibrated with measured dynamic characteristics. These characteristics are the periods and the associated mode shapes of vibration and the modal damping coefficients. This paper deals with the measurements and the interpretation of the results of ambient vibration tests done on a newly erected cable stayed bridge across the Oued Dib River at Mila city in Algeria. The signal analysis of ambient vibration records will permit to determine the dynamic characteristics of the bridge. On the other hand, a 3-D model of the bridge is developed in order to assess the frequencies and the associated modes of vibration. This information will be necessary in the planning of the test on the site (locations of the sensors, frequencies to be measured and the associated mode shapes of vibration). The frequencies predicted by the finite element model are compared with those measured during full-scale ambient vibration measurements of the bridge. In the same way, the modal damping coefficients obtained by the random decrement method are compared to those of similar bridges.
Velamo do Campo: Its Volatile Constituents, Secretory Elements, and Biological Activity
Fatiha El Babili,Christine Roques,Laila Haddioui,Floriant Bellvert,Ce´dric Bertrand,Christian Chatelain 한국식품영양과학회 2012 Journal of medicinal food Vol.15 No.7
The volatile components from Croton campestris root bark were localized by an anatomical study and analyzed by gas chromatography–mass spectrometry for the first time. The roots of this plant showed secretory cells. These volatile constituents, isolated from the dichloromethane extract by hydrodistillation, were analyzed by gas chromatography–mass spectrometry. We found 69 components. They were characterized, and the major constituents of crude oil root barks were spathulenol (23.3%) and borneol (18.7%). Growth inhibitory activity of the active compounds in solution was evaluated by measuring minimal inhibitory concentrations using a broth micromethod. The minimal inhibitory concentration of root bark volatile constituents was 1.56 lg/mL for Staphylococcus aureus, 3.125 lg/mL for Candida albicans, and 6.25 lg/mL for Aspergillusniger.
Antagonistic Effect of a Cytoplasmic Domain on the Basal Activity of Polymodal Potassium Channels
Soussia, Ismail Ben,Choveau, Frank S.,Blin, Sandy,Kim, Eun-Jin,Feliciangeli, Sylvain,Chatelain, Franck C.,Kang, Dawon,Bichet, Delphine,Lesage, Florian Frontiers Media S.A. 2018 Frontiers in molecular neuroscience Vol.11 No.-
<P>TREK/TRAAK channels are polymodal K<SUP>+</SUP> channels that convert very diverse stimuli, including bioactive lipids, mechanical stretch and temperature, into electrical signals. The nature of the structural changes that regulate their activity remains an open question. Here, we show that a cytoplasmic domain (the proximal C-ter domain, pCt) exerts antagonistic effects in TREK1 and TRAAK. In basal conditions, pCt favors activity in TREK1 whereas it impairs TRAAK activity. Using the conformation-dependent binding of fluoxetine, we show that TREK1 and TRAAK conformations at rest are different, and under the influence of pCt. Finally, we show that depleting PIP<SUB>2</SUB> in live cells has a more pronounced inhibitory effect on TREK1 than on TRAAK. This differential regulation of TREK1 and TRAAK is related to a previously unrecognized PIP<SUB>2</SUB>-binding site (R329, R330, and R331) present within TREK1 pCt, but not in TRAAK pCt. Collectively, these new data point out pCt as a major regulatory domain of these channels and suggest that the binding of PIP<SUB>2</SUB> to the pCt of TREK1 results in the stabilization of the conductive conformation in basal conditions.</P>
Siqueira-Neto, Jair L.,Moon, Seunghyun,Jang, Jiyeon,Yang, Gyongseon,Lee, Changbok,Moon, Hong Kee,Chatelain, Eric,Genovesio, Auguste,Cechetto, Jonathan,Freitas-Junior, Lucio H. Public Library of Science 2012 PLoS neglected tropical diseases Vol.6 No.6
<▼1><P>Leishmaniasis is a tropical disease threatening 350 million people from endemic regions. The available drugs for treatment are inadequate, with limitations such as serious side effects, parasite resistance or high cost. Driven by this need for new drugs, we developed a high-content, high-throughput image-based screening assay targeting the intracellular amastigote stage of different species of <I>Leishmania</I> in infected human macrophages. The <I>in vitro</I> infection protocol was adapted to a 384-well-plate format, enabling acquisition of a large amount of readouts by automated confocal microscopy. The reading method was based on DNA staining and required the development of a customized algorithm to analyze the images, which enabled the use of non-modified parasites. The automated analysis generated parameters used to quantify compound activity, including infection ratio as well as the number of intracellular amastigote parasites and yielded cytotoxicity information based on the number of host cells. Comparison of this assay with one that used the promastigote form to screen 26,500 compounds showed that 50% of the hits selected against the intracellular amastigote were not selected in the promastigote screening. These data corroborate the idea that the intracellular amastigote form of the parasite is the most appropriate to be used in primary screening assay for <I>Leishmania</I>.</P></▼1><▼2><P><B>Author Summary</B></P><P>Leishmaniasis, one of the most neglected tropical diseases, affects over 2 million people each year. Visceral leishmaniasis (VL), also known as Kala-azar, is caused by the protozoan parasites <I>Leishmania donovani</I> and <I>Leishmania infantum</I> and is fatal if left untreated. Because existing treatments are often ineffective due to parasite resistance and/or toxicity new drugs are urgently needed. Leishmaniasis is transmitted to humans by the bite of an infected sandfly. In the insect vector, parasites exist as flagellated forms—promastigotes, which infect macrophage cells of the human host, where they differentiate to round forms known as amastigotes. Amastigotes and promastigotes are substantially different from a molecular perspective. Drug discovery for leishmaniasis has traditionally been complicated by the unavailability of validated drug targets and of relevant drug assays. Whole cell-based assays have been widely used, as they bypass the need for a validated target. However, they use the insect form of the parasite; indeed, the human form, the intracellular amastigote, is difficult to obtain in the laboratory in quantities compatible with drug screening. We describe here the technical advances that made it possible to adapt the intracellular amastigote form of <I>L. donovani</I> to a drug assay compatible with high-throughput screening.</P></▼2>