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( Bao Le ),( Gyuhwa Chung ),( Seung Hwan Yang ) 한국응용생명화학회 2018 Journal of Applied Biological Chemistry (J. Appl. Vol.61 No.3
Chitinases are glycosyl hydrolases which cleave the β- 1,4 linkage of chitin into oligo or monomers of N-acetylglucosamine. These bacterial enzymes have been used for a wide range of applications in the food and pharmaceutical industries. In this study, we isolated two potential chitinolytic strains, BAO-01 and BAO-02, from salt-fermented shrimp, which were shown to belong to the genus Salinivibrio through genetic characterization using 16S rRNA. These isolates were gram-positive, rod-shaped, and non-spore forming. BAO-01 showed greater growth and chitinase activity than BAO-02 after the incubation at 37 oC for 4 days. Both strains grew on a wide range of carbon and nitrogen sources, pH values, temperatures, and salt levels. However, they showed minor biochemical differences. In addition, their antimicrobial activities against foodborne pathogens and antibiotic susceptibilities were evaluated. These Salinivibrio spp. did not show bioamine production, hemolytic activity, and mucin degradation. Therefore, the in vitro screening results suggested that these bacteria could be widely used as new candidates for chitin hydrolyzation and seafood fermentation.
Bao‑Jian Zhu,Lin Tang,Ying‑Ying Yu,Dao‑Jun Wang,Chao‑Liang Liu 한국유전학회 2017 Genes & Genomics Vol.39 No.6
Ecdysteroids play an important role in the regulation of molting process in crustaceans. The suppression subtractive hybridization (SSH) technique was employed to search for 20-hydroxyecdysone-responsive genes in the hepatopancreas of the crayfish Procambarus clarkii. Ninety-three putative expressed sequence tags were identified by SSH, including three immune response-related genes, two cell cycle and apoptosis genes, four respiration and energy metabolism genes, four transport-related genes, six metabolism-related genes, two stress response genes, and eight transcription and translation regulation genes. The expression levels of the examined genes were regulated by 20-hydroxyecdysone and varied in the hepatopancreas during the molting stages; data were confirmed by real-time PCR. RNAi of the ecdysteroid receptor had significant effects on the expression levels of 20-hydroxyecdysone- responsive genes. These differentially expressed genes identified by SSH will provide insight into the ecdysteroid signaling pathway in P. clarkii.
Pham Thi Ngoc Anh,Bao Le,양승환 한국응용생명화학회 2020 Journal of Applied Biological Chemistry (J. Appl. Vol.63 No.3
This study focused on the preparation and characterization of anti-wrinkle peptides from the salted and fermented shrimp (Acetes japonicus) inoculated with Salinivibrio cibaria BAO-01 (SFSC). The results showed the proximate composition of SFSC to be 9.23% water, 75.32% protein, 0.23% fat, and 13.3 mg/g ash. Interestingly, the S. cibaria fermentation significantly increased the amount of methionine, leucine, and arginine. The in vitro antioxidant activity was assayed by the diphenylpicrylhydrazyl method and its IC50 value was found to be 43.02±2.84 μg/mL. It was observed to inhibit the activity of elastase, tyrosinase, collagenase, and hyaluronidase. The IC50 values of SFSC were 182.75±12.38 μg/mL for anti-elastase activity, 186.78±7.95 μg/mL for anti-tyrosinase activity, 444.4±34.81 μg/mL for anti-collagenase activity, and 1447.95±28.92 μg/mL for anti-hyaluronidase activity. These results suggest that salted and fermented shrimp has strong potential for the development of nutricosmetic products.
張寶秀 서강대학 지역발전연구소 1996 지역발전연구 : 서강전문대 Vol.2 No.-
A city is the political, cultural, and economic centre of the region aroud it. A good urban system of a region can promote the development of the whole region. Yulin Prefecture, in the southeast Guangxi Zhuangzu Autonomous Region, is a richly endowed area. The urban system in Yulin Prefecture has been developing naturally for more than 2000 years, but now its some aspects, such as the centural city's scale, the scale structure, the functional division of the cities and towns, etc., are disadvantageous to the economic development of Yulin Prefecture. So the urban system need to be planned in order to promote effectively the regional overall prosperity. It is what this paper studies. The paper is divided into four sections: Ⅰ. Introduction Ⅱ. The Process of the Urban System Development Ⅲ. The Motive Forces of the Urban System Development Ⅳ. Conclusion - The Planning Developmental Direction of the Urban System Development
Coffee Husk By-Product as Novel Ingredients for Cascara Kombucha Production
Bao Xuyen Nguyen Le,Thach Phan Van,Quang Khai Phan,Gia Bao Pham,Hoa Pham Quang,Anh Duy Do The Korean Society for Microbiology and Biotechnol 2024 Journal of microbiology and biotechnology Vol.34 No.3
Kombucha, a fermented beverage, is gaining popularity due to its numerous beneficial health effects. Various substrates such as herbs, fruits, flowers, and vegetables, have been used for kombucha fermentation in order to enhance the flavor, aroma, and nutritional composition. This study aims to investigate the potential suitability of cascara as a novel ingredient for kombucha production. Our findings suggested that cascara is a suitable substrate for kombucha production. Fermentation elevated the total phenolic and flavonoid content in cascara, which enhanced the antioxidant, antibacterial, and prebiotic characteristics of the product. Furthermore, the accumulation of acetic acid-induced the pH lowering reached 2.7 after 14 days of fermentation, which achieved the microbiological safety of the product. Moreover, 14 days of fermentation resulted in a balanced amalgamation of acidity, sweetness, and fragrance according to sensory evaluation. Our findings not only highlight the potential of cascara kombucha as a novel substrate for kombucha production but also contribute to repurposing coffee by-products, promoting environmentally friendly and sustainable agricultural development.