Evaluation of Bio-Control Efficacy of Trichoderma Strains against Alternaria alternata Causing Leaf Blight of Ashwagandha [Withania somnifera (L.) Dunal]

Md. Ahsanur Rahman,Md. Arifur Rahman,Zakiah Rahman Moni,Mohammad Anisur Rahman 강원대학교 산림과학연구소 2020 Journal of Forest Science Vol.36 No.3

Ashwagandha is an important ancient medicinal crops, being affected with many diseases, among which leaf blight caused by Alternaria alternata has become the constraint resulting in huge yield losses. Continuous usage of chemical methods leads to environment, soil and water pollution. Whereas biological control of diseases is long lasting, inexpensive, eco-friendly and harmless to target organisms. In this context, it is aimed to evaluate five Trichoderma strains viz. Trichoderma virens IMI-392430, T. pseudokoningii IMI-392431, T. harzianum IMI-392432, T. harzianum IMI-392433 and T. harzianum IMI-392434 as bio-control efficacy against A. alternata and growth promoting effect in Ashwagandha. All the Trichoderma strains had varied antagonistic effects against the pathogen. In dual culture technique, the strain T. harzianum IMI-392433 showed maximum percentage inhibition of mycelial growth (54.89%) followed by T. harzianum IMI-392432 (53.83%), T. harzianum IMI-392434 (48.94%) and T. virens IMI-392430, (43.62%) against the pathogen, while the least inhibition percentage was observed with the T. pseudokoningii IMI-392431 (36.60%). The culture filtrate of the Trichoderma strain, T. harzianum IMI-392433 recorded highest inhibition on the mycelial growth (39.05%) and spore germination (80.77%) of pathogen and the lowest was recorded in T. pseudokoningii IMI-392431 (20.45 and 50%). Moreover, seeds treated with spore suspension of the strain T. harzianum IMI-392433 reduced the percentages of disease severity index significantly. The strain T. harzianum IMI-392433 also significantly increased seed germination %, seedling vigor and growth of Ashwagandha. The correlation matrix showed that root yield per plant of Ashwagandha had significant and positive correlation with plant height (r=0.726**), number of leaf (r=0.514**), number of primary branch (r=0.820**), number of secondary branch (r=0.829**), fresh plant weight (r=0.887**), plant dry weight (r=0.613**), root length (r=0.824**), root diameter (r=0.786**), root dry weight (r=0.739**) and fresh root weight (r=0.731**). The significant and negative correlation (r=-0.336**) was observed with the root yield and percentages of disease severity index. The study recognized that the T. harzianum IMI-392433 strain performed well in inhibiting the mycelial growth and reduced the percentages of disease severity index of pathogen as well as increased the plant growth in Ashwagandha.

A Novel Method for Patterning of Poly(3,4-ethylenedioxythiophene) Films Using UV Exposure-Activated Self-Assembled Monolayers

Rahman, Mohammad Arifur,Kwon, Taewook,Lee, Young Kyu,Rahim, Abdur,Nam, Hosoek,Hong, Joo-Wha,Lee, Jong-Kwon,Soh, Hoesup,Lee, Jaegab American Scientific Publishers 2012 Journal of Nanoscience and Nanotechnology Vol.12 No.2

Ligand-based virtual screening, consensus molecular docking, multi-target analysis and comprehensive ADMET profiling and MD stimulation to find out noteworthy tyrosine kinase inhibitor with better efficacy and accuracy

Arifur Rahman,Nazmul Hasan Naheed,Sabreena Chowdhury Raka,Nazmul Qais,AZM Ruhul Momen 경희대학교 융합한의과학연구소 2020 Oriental Pharmacy and Experimental Medicine Vol.20 No.4

Inhibition of BCR–ABL tyrosine kinase plays a crucial role in the management of chronic myelogenous leukemia (CML). The suppression of CML is getting harder because of a distinct pattern of resistance. Developing new types of ABL tyrosine kinase inhibitors along with ABL2, CSF1R, KIT, LCK, PDGFRA, and PDGFRB inhibitors is the main objective of this study that may overcome the drug resistance issue. The current study has been conducted using a kinase database containing 177,000 bioactive molecules, the top 135 molecules were selected with the best docking score and subjected to comprehensive ADMET profiling, multi-target analysis. Based on consensus molecular docking score (AutoDock, Chimera, Achilles, and Mcule), 22 molecules have been screened out which later undertaken for ADME/T profiling. After profiling of ADME/T data, selected molecules subjected to docking with multiple targets. Finally, molecular dynamics simulations had performed to screen the binding accuracy of the four lead molecules with ABL1. MD simulations of the desired complex (ABL1, ABL2, CSF1R, KIT, LCK, PDGFRA, and PDGFRB, among them ABL1 was the prime target) performed and found that PCID 10181160 and PCID 72724706 are the most promising inhibitors comparing to imatinib. These lead molecules are the potential CML inhibitors that could resolve the resistance pattern. Further chemical synthesis, wet lab analysis, and experimental validation deserve the utmost attention.

High Performance Flexible Organic Thin Film Transistors (OTFTs) with Octadecyltrichlorsilane/Al₂O₃/Poly(4-vinylphenol) Multilayer Insulators

Rahman, Mohammad Arifur,Kim, Hyunho,Lee, Young Kyu,Lee, Chiyoung,Nam, Hosoek,Lee, Jang-Sik,Soh, Hoesup,Lee, Jong-Kwon,Lee, Eun-Gu,Lee, Jaegab American Scientific Publishers 2012 Journal of Nanoscience and Nanotechnology Vol.12 No.2

An adaptive network allocation vector timer-based carrier sense multiple access with collision avoidance medium access control protocol for underwater acoustic sensor networks

Rahman, Md Arifur,Lee, Youngdoo,Koo, Insoo SAGE Publications 2017 International journal of distributed sensor networ Vol.13 No.1

A novel low molecular weight endo-xylanase from Streptomyces sp. CS628 cultivated in wheat bran.

Rahman, Md Arifur,Choi, Yun Hee,Pradeep, G C,Choi, Yoon Seok,Choi, Eun Joo,Cho, Seung Sik,Yoo, Jin Cheol Humana Press 2014 Applied biochemistry and biotechnology Vol.173 No.6

<P>An extracellular low molecular weight xylanase (Xyn628) from Streptomyces sp. CS628 was isolated from Korean soil sample, produced in wheat bran medium, purified, and biochemically characterized. Xyn628 was purified 4.8-fold with a 33.78?% yield using Sepharose CL-6B column chromatography. The purified xylanase was ~18.1?kDa estimated by SDS-PAGE and xylan zymography. N-terminal amino acid sequences of Xyn628 were AYIKEVVSRAYM. The enzyme was found to be stable in a broad range of pH (5.0-13.0) and up to 60?C and have optimal pH and temperature of pH?11.0 and 60?C, respectively. Xyn628 activities were remarkable affected by various detergents, chelators, modulators, and metal ions. The xylanase produced xylobiose and xylotriose as principal hydrolyzed end products from the xylan. It was found to degrade agro-waste materials like corn cob and wheat bran by Xyn628 (20?U/g) as shown by electron microscopy. As being simple in purification, low molecular weight, alkaline, thermostable, and ability to produce xylooligosaccharides show that Xyn628 has potential applications in bioindustries as a biobleaching agent or/and xylooligosaccharides production with an appropriate utilization of agro-waste.</P>

An ammonium sulfate sensitive chitinase from Streptomyces sp. CS501

Md. Arifur Rahman,최윤희,G. C. Pradeep,유진철 대한약학회 2014 Archives of Pharmacal Research Vol.37 No.12

A chitinase from Streptomyces sp. CS501 wasisolated from the Korean soil sample, purified by singlestepchromatography, and biochemically characterized. The extracellular chitinase (Ch501) was purified to 4.60fold with yield of 28.74 % using Sepharose Cl-6B column. The molecular mass of Ch501 was approximately 43 kDaas estimated by SDS-PAGE and zymography. The enzyme(Ch501) was found to be stable over a broad pH range(5.0–10.0) and temperature (up to 50 C), and have anoptimum temperature of 60 C. N-terminal sequence ofCh501 was AAYDDAAAAA. Intriguingly, Ch501 washighly sensitive to ammonium sulfate but it’s completelysuppressed activity was recovered after desalting out. TLCanalysis of Ch501 showed the production of N-acetyl Dglucosamine(GlcNAc) and Diacetylchitobiose (GlcNAc)2,as a principal hydrolyzed product. Ch501 shows antifungalactivity against Fusarium solani and Aspergillus brasiliensis,which can be used for the biological control offungus. As has been simple in purification, stable in a broadrange of pH, ability to produce oligosaccharides, andantifungal activity showed that Ch501 has potential applicationsin industries as for chitooligosaccharides productionused as prebiotics and/or for the biological control ofplant pathogens in agriculture.

Selective Deposition of Copper With Iodine Assisted Growth of MOCVD on an MPTMS Monolayer Surface at a Low Temperature

( Mohammad Arifur Rahman ),( Hee Jung Park ),( Ara Kim ),( Chi Young Lee ),( Jaegab Lee ) 대한금속재료학회 ( 구 대한금속학회 ) 2010 ELECTRONIC MATERIALS LETTERS Vol.6 No.4

We have investigated the selective deposition of Cu films on the mercaptopropyltrimethoxysilane-ocatade-cyltrichlorosilane (MPTMS-OTS) patterned glass substrate using (hfac)Cu(DMB) and C2H5I as precursors at 110°C. The low temperature deposition of Cu without iodine exhibited the difficulties of Cu nucleation on MPTMS with its low Cu growth rate of 22 nm/min and long induction period of approximately 4 min at 110°C. The addition of iodine significantly enhanced the surface diffusion of Cu adatoms on the MPTMS surface and thus led to increased nucleation and growth rate (28 nm/min) at 110°C. With these advantages, we successfully increased the maximum thickness of Cu selectively deposited on the MPTMS patterned lines from 57 nm to 140 nm without selectivity loss by adding iodine to (hfac)Cu(DMB)(3,3-dymethyl-1-butene) at 110°C. Consequently, the low temperature process for the selective deposition of Cu on the MPTMSOTS patterned surface can be utilized for the fabrication of flexible electronics.

Discrete time Adaptive Controller for Suppression of Resonance in Hard Disk Drive Servo System

Md. Arifur Rahman,Abdullah Al Mamun,Kui Yao 제어·로봇·시스템학회 2015 International Journal of Control, Automation, and Vol.13 No.5

Mechanical resonant modes of the actuator severely impede the efforts in achieving high precision in head positioning servo system of hard disk drives (HDD). Hence it is important that these modes be properly compensated. The conventional approach is to use a notch filter in cascade with the nominal controller, which works well if the resonant frequencies are known and do not vary during operation. However, in reality, frequency and damping ratio of the resonant modes vary in mass-produced systems. They also get changed over time due to wear and tear. As a result, auto-tuning or adaptation of the notch filters is required to maintain the performance at the same level. In this paper, a discrete time adaptive controller is presented in a simple and yet efficient way to suppress the effects of resonant modes. Effectiveness of the proposed compensator is demonstrated through simulation and experiments by implementing the compensator for a Voice Coil Motor (VCM) actuator used in HDD.

An Alkaline and Metallo-protein Type Endo Xylanase from Streptomyces sp. CSWu-1

Md. Arifur Rahman,최연희,Pradeep G. C.,최윤석,최은주,조승식,송재경,유진철 한국생물공학회 2014 Biotechnology and Bioprocess Engineering Vol.19 No.2

An alkaline xylanase (XynWu-1) fromStreptomyces sp. CSWu-1 was isolated from the Koreansoil sample, purified and biochemically characterized. Theextracellular xylanase was purified 4.8 fold with a 16%yield using Sephadex G-50 followed by DEAE-Sepharose(fast flow) column chromatography. The molecular massof the enzyme was approximately 37 kDa estimated bySDS-PAGE and xylan zymography. N-terminal amino acidsequence of XynWu-1 was AINVLVAALX. The enzymewas found to be stable in a broad range of pH (7.0 ~ 13.0)and to 50°C and have an optimal pH and temperature of11.0 and 60°C, respectively. XynWu-1 activity was foundto be affected by Mn2+ ion with highest activity at 6 mMand produced xylose, xylobiose, and xylotetraose as majorhydrolyzed end products. It was found to degrade agrowaste materials like corncob and wheat bran by XynWu-1(2,000 U/g) as shown by electron microscopy. As beingstable in extreme alkaline pH, diverse peculiar biochemicalcharacteristics, and ability to produce oligosaccharide showsthat XynWu-1 has potential application in various bioindustrieslike probiotics, ethanol, etc.