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An Xueying,Wang Rongliang,Lv Zhongyang,Wu Wenshu,Sun Ziying,Wu Rui,Yan Wenjin,Jiang Qing,Xu Xingquan 생화학분자생물학회 2024 Experimental and molecular medicine Vol.56 No.-
Osteoarthritis (OA) is the most common form of arthritis. However, the exact pathogenesis remains unclear. Emerging evidence shows that N6-methyladenosine (m6A) modification may have an important role in OA pathogenesis. This study aimed to investigate the role of m6A writers and the underlying mechanisms in osteoarthritic cartilage. Among m6A methyltransferases, Wilms tumor 1-associated protein (WTAP) expression most significantly differed in clinical osteoarthritic cartilage. WTAP regulated extracellular matrix (ECM) degradation, inflammation and antioxidation in human chondrocytes. Mechanistically, the m6A modification and relative downstream targets in osteoarthritic cartilage were assessed by methylated RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing, which indicated that the expression of frizzled-related protein (FRZB), a secreted Wnt antagonist, was abnormally decreased and accompanied by high m6A modification in osteoarthritic cartilage. In vitro dysregulated WTAP had positive effects on β-catenin expression by targeting FRZB, which finally contributed to the cartilage injury phenotype in chondrocytes. Intra-articular injection of adeno-associated virus-WTAP alleviated OA progression in a mouse model, while this protective effect could be reversed by the application of a Wnt/β-catenin activator. In summary, this study revealed that WTAP-dependent RNA m6A modification contributed to Wnt/β-catenin pathway activation and OA progression through post-transcriptional regulation of FRZB mRNA, thus providing a potentially effective therapeutic strategy for OA treatment.
Xueying Li,He Huang,Bing Xu,Hongqiang Guo,Yingcheng Lin,Sheng Ye,Jiqun Yi,Wenyu Li,Xiangyuan Wu,Wei Wang,Hongyu Zhang,Derong Xie,Jiewen Peng,Yabing Cao,Xingxiang Pu,Chengcheng Guo,Huangming Hong,Zhao 대한암학회 2019 Cancer Research and Treatment Vol.51 No.3
Purpose Rituximab with cyclophosphamide, doxorubicin, vincristine, and prednisone administered every 3 weeks (R-CHOP-21) is the standard care for diffuse large B-cell lymphoma (DLBCL). It is unknown whether the dose-dense R-CHOP (R-CHOP-14) could improve the outcome of the disease in Asian population. Materials and Methods Newly diagnosed DLBCL patients were centrally, randomly assigned (1:1) to receive R-CHOP- 14 or R-CHOP-21. R-CHOP-14 was administered every 2 weeks, and R-CHOP-21 was administered every 3 weeks. Primary end point was disease-free survival (DFS). Secondary end points included overall survival (OS), progression-free survival (PFS), response rate and toxicities. Results Seven hundred and two patients were randomly assigned to receive R-CHOP-14 (n=349) or R-CHOP-21 (n=353). With a median follow-up of 45.6 months, the two groups did not differ significantly in 3-year DFS (79.6% for R-CHOP-14 vs. 83.2% for R-CHOP-21, p=0.311), 3-year OS (77.5% for R-CHOP-14 vs. 77.6% for R-CHOP-21, p=0.903), or 3-year PFS (63.2% for R-CHOP-14 vs. 66.1% for R-CHOP-21, p=0.447). Patients with an International Prognostic Index (IPI) score ! 2 had a poorer prognosis compared to those with an IPI score < 2. Grade 3/4 hematologic and non-hematologic toxicities were manageable and similar between R-CHOP-14 and R-CHOP-21. Conclusion R-CHOP-14 did not improve the outcome of DLBCL compared to R-CHOP-21 in Asian population. With manageable and similar toxicities, both of the two regimens were suitable for Asian DLBCL patients. For high-risk patients with IPI ! 2, new combination regimens based on R-CHOP deserve further investigation to improve efficacy.
Xueying Chai,Zhilu Ai,Jun Liu,Ting Guo,Jingyan Wu,Jie Bai,Qinlu Lin 한국식품과학회 2020 Food Science and Biotechnology Vol.29 No.7
The effects of the secondary metabolite biosynthesison the metabolism and morphology of the Monascuspurpureus were investigated in this study. Hypha andseptum length became longer after deletion of genes pigRand pksCT in M. purpureus LQ-6 by Agrobacteriumtumefaciens-mediated transformation technology, highlybranched hyphae, much smaller and freely dispersedmycelial pellets were observed in M. purpureus. Comparedwith that in the wild-type, the level of intracellular NADHand NADPH was almost constant in M. purpureus DpigRat 4 days, but the NADH and NADPH levels decreased by1.58-fold and 3.71-fold in M. purpureus DpksCT. Thepresent study can not only provide a kind of strategy toimprove the Monascus pigments production, but also providetheoretical support for the further study of relationshipbetween the secondary metabolites, metabolism and morphologicalchange.
Xueying Tian 보안공학연구지원센터 2016 International Journal of Hybrid Information Techno Vol.9 No.2
The economy in Suzhou, China is in the late stages of its industrialisation, during which, the completeness of non-profit organizations (NPOs) has been an important indicator for measuring the all-round socio-economic development of Suzhou. Currently, the NPOs of Suzhou make up for deficiencies in the government and the market, and thus make a positive contribution to the political and economic development of Suzhou. However, it is easy to find that NPOs still suffer disadvantages such as slow development, rarity, and low capacity: these inhibit their active benefits. This research investigated the classification and development status of the NPOs in Suzhou; on this basis, it analyzed the main problems facing them. Moreover, aiming at these problems, countermeasures and suggestions were proposed so as to allow them to play a more important in the socio-economic system of Suzhou.
Adsorption of dyes from water by Prunella vulgaris stem and subsequent fungal decolorization
Xueying Zhang,Jun Zhou,Yuben Fan,Jiayang Liu 한국화학공학회 2020 Korean Journal of Chemical Engineering Vol.37 No.9
The residue of herbaceous Prunella vulgaris stem (PVS) was evaluated as a potential adsorbent for dye removal, followed by fungal cultivation to achieve dye degradation on solid waste. PVS was analyzed in terms of nutritional composition such as fiber, ash, protein, and fat, which not only played a role in dye adsorption but also provided solid matrix for fungal growth. Five dyes, namely, crystal violet (CV), methylene blue (MB), reactive black 5 (RB), indigo carmine (IC), and direct red 80 (DR), were tested as adsorbates but only CV and MB were effectively adsorbed. Effect of sorbent dose, contact time, dye concentration, and NaCl on adsorption was investigated individually. Langmuir model was suitable for fitting MB adsorption, while adsorption of CV adopted the Freundlich model. The adsorption capacity was calculated to be 625 mg/g for CV and 303 mg/g for MB, respectively. The adsorption process of both dyes was spontaneous and endothermic, and the adsorption followed pseudo 2nd order kinetic model and film diffusion model. The dyed PVS was finally cultivated with fungus Pycnoporus sp., wherein efficient dye decolorization was attained under solid state fermentation. As such, PVS coupled with subsequent fungal degradation might serve as novel alternative for dye effluent treatment.
Xueying Cui,송해덕,김연경,Jung Won Hur 숭실대학교 영재교육연구소 2019 Global Creative Leader Vol.9 No.6
This study aims to analyze the degree that online task value and collaboration preference can predict learning engagement, and to verify the mediating effects of self-regulated learning ability in flipped learning. This was done in terms of both pre-class and in-class of flipped learning, which are actively applied in higher education. To this end, a survey of 220 college students taking a flipped learning class at C University in Seoul was conducted. The study’s findings showed that online task value and collaboration preference had a significant effect on learning engagement, while also predicting self-regulated learning ability. Self-regulated learning ability was found to affect learning engagement, and was also found to partially mediate the relationships of both online task value and collaboration preference in relation to learning engagement.
Xueying Tao(도설영),Wu Jing(오청),Kyoung-Sook Kim(김경숙),Ziniu Yu(유자우),Young-Choon Lee(이영춘) 한국생명과학회 2008 생명과학회지 Vol.18 No.5
해양으로부터 분리한 한천분해효소를 생산하는 미생물을 분리하여 동정한 결과 이 세균은 Pseudoalteromonas sp.로 판명되어 Pseudoalteromonas sp. JT-6로 명명하였다. 또한 이 균주로부터 한천분해효소 유전자를 클로닝하여 염기서열을 결정하였던 바, 이 유전자는 445개의 아미노산을 코드하는 1338 bp의 염기서열로 이루어져 있었다. 이 유전자는 Janthinobacterium sp. SY12와 99%의 높은 아미노산 서열 상동성을 나타내었고 N-말단으로부터 신호서열, 당분해효소 family 16에 속하는 β-agarase 촉매영역 및 탄수화물 결합영역으로 이루어진 도메인 구조를 나타내었다. 대장균에서 생산되어 정제된 재조합 한천분해효소는 40℃와 pH 9.0에서 최대 활성을 나타내었으며, 기질인 한천분해 형태로 볼 때 본 효소는 endo-type의 β-agarase임이 증명되었다. A gene (agaA6) encoding an extracellular agarase from a marine bacterium, Pseudoalteromonas sp. JT-6, was cloned, sequenced and expressed in Escherichia coli. It comprised an open reading frame of 1338 base pairs and encodes a protein of 445 amino acids with a predicted molecular weight of 50,150 daltons. The entire amino acid sequence of this agarase gene showed 99% identity with the agaA gene from Janthinobacterium sp. SY12. It consists of a signal peptide, a glycoside hydrolase family 16 β-agarase domain and a carbohydrate-binding domain. The recombinant agarase was overexpressed and purified to homogeneity. Enzyme activity analysis revealed that the optimum temperature and pH for the purified recombinant enzyme were around 40℃ and 9.0. The enzyme was an endo-type β-agarase and hydrolyzed agarose to several oligosaccharides.