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문병현,송주열,이현성,손경규,엄태환,김정우,김아름 대구대학교 정보통신연구소 2004 情報通信硏究 Vol.3 No.2
The system designed in this paper, can mark the interior state of greenhouse using sensors of temperature, humidity and illumination on greenhouse interior by greenhouse system for farm village area's agriculture automation. And, the real time monitor is possible real time monitoring by camera. Also, system that can supply water using spring cooler system and humidifier. The proposed system can sustain a designed environment of the greenhouse through rentilation fan, fluorescent light and roof door control. TCP/IP is used for the remote control of state the sensors in the client program. 본 논문에서 설계된 시스템은 농촌 지역의 농업 자동화를 위한 온실 시스템으로 온실 내부에 온도, 습도, 조도 센서를 이용한 온실 내부의 상태를 표시할 수 있으며 카메라를 이용하여 실시간 관측이 가능하다. 또한 본 시스템은 지붕 개폐, 스프링 쿨러와 가습기를 이용한 수분 공급, 환풍기, 형광등 등을 통하여 온실 내부의 환경을 유지시킬 수 있다. 모든 센서를 이용한 상태와 온실 제어를 TCP/IP를 이용한 클라이언트 프로그램에서 원격으로 관리할 수 있다.
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Biosynthesis of Glycosylated Derivatives of Tylosin in Streptomyces venezuelae
( Ah Reum Han ),( Sung Ryeol Park ),( Je Won Park ),( Eun Yeol Lee ),( Dong Myung Kim ),( Byung Gee Kim ),( Yeo Joon Yoon ) 한국미생물 · 생명공학회 2011 Journal of microbiology and biotechnology Vol.21 No.6
Streptomyces venezuelae YJ028, bearing a deletion of the entire biosynthetic gene cluster encoding the pikromycin polyketide synthases and desosamine biosynthetic enzymes, was used as a bioconversion system for combinatorial biosynthesis of glycosylated derivatives of tylosin. Two engineered deoxysugar biosynthetic pathways for the biosynthesis of TDP-3-O-demethyl-D-chalcose or TDP-Lrhamnose in conjunction with the glycosyltransferaseauxiliary protein pair DesVII/DesVIII were expressed in a S. venezuelae YJ028 mutant strain. Supplementation of each mutant strain capable of producing TDP-3-O-demethyl- D-chalcose or TDP-L-rhamnose with tylosin aglycone tylactone resulted in the production of the 3-O-demethyl- D-chalcose, D-quinovose, or L-rhamnose-glycosylated tylactone.
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( Ah Reum Khang ),( Eun Ki Kim ),( Eun Shil Hong ),( Hyung Jin Choi ),( Chan Soo Shin ),( Kyong Soo Park ),( Seong Yeon Kim ) 대한내과학회 2011 대한내과학회 추계학술발표논문집 Vol.2011 No.1
Parathyroid cysts are very rare among the causes of primary hyperparathyroidism (PHPT). They are divided into functional and nonfunctional cysts. Operative removal is a treatment of choice for a functional cyst. 99mTc-Sestamibi parathyroid scan is a highly effective and sensitive diagnostic tool for localization of hyperparathyroidism, however it shows a false-negative result, occasionally. We found one case which was presumed to have cystic parathyroid adenoma based on clinical findings and neck computed tomography (CT) with negative finding in parathyroid scan. A male patient who was 44 years-old visited to hospital due to legs pain and it was occurred 5months ago and deteriorated gradually. In addition, he presented with the compressive symptoms such as dysphagia and hoarseness. Serum calcium level was 14.4 mg/dl, phosphorus 2.0 mg/dl, creatinine 0.99 mg/dl and intact parathyroid hormone (iPTH) was increased to 478.1 pg/ml. On neck CT, 6.2×3.8×2.7 cm sized cystic nodule was found in inferior part of right thyroid gland. Sestamibi uptake for the found nodule was not detected on 2 hour delayed imaging of 99mTc-Sestamibi parathyroid scan. Fine needle aspiration for diagnosis and localization was done and intracystic iPTH was increased to 61,600 pg/ml. The iPTH monitoring for focused parathyroidectomy led to successful enucleation of right inferior parathyroid. It was a parathyroid adenoma and serum calcium, phosphate and iPTH was normalized after operation. A cystic parathyroid adenoma can be hardly detected on 99mTc-Sestamibi parathyroid scan sometimes. The multidisciplinary approach which considered clinical findings, laboratory results, physical examination and other imagings (CT, Ultrasound, etc) is important for differential diagnosis of hyperparathyroidism. The iPTH monitoring helps the focused parathyroidectomy.
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Kim, Shin Hye,Kim, Ah Reum,Choi, Hyun Seok,Kim, Min Young,Chun, Eun Hi,Oh, Seung-Ha,Choi, Byung Yoon Williams & Wilkins Co 2015 Medicine Vol.94 No.43
<P><B>Abstract</B></P><P>Unilateral sensorineural hearing loss (USNHL)/single-side deafness (SSD) is a frequently encountered disability in children. The etiology of a substantial portion of USNHL/SSD still remains unknown, and genetic causes have not been clearly elucidated. In this study, the authors evaluated the heritability of USNHL/SSD.</P><P>The authors sequentially recruited 50 unrelated children with SSD. For an etiologic diagnosis, we performed a rigorous review on the phenotypes of family members of all children and conducted, if necessary, molecular genetic tests including targeted exome sequencing of 129 deafness genes.</P><P>Among the 50 SSD children cohort, the authors identify 4 (8%) unrelated SSD probands from 4 families (SH136, SB173, SB177, and SB199) with another hearing impaired family members. Notably, all 4 probands in our cohort with a familial history of SSD also have pigmentary abnormalities such as brown freckles or premature gray hair within first degree relatives, which may indicate that genes whose products are involved with pigmentary disorder could be candidates for heritable SSD. Indeed, SH136 and SB199 turned out to segregate a mutation in <I>MITF</I> and <I>PAX3</I>, respectively, leading to a molecular diagnosis of Waardenburg syndrome (WS).</P><P>We report, for the first time in the literature, a significant heritability of pediatric SSD. There is a strong association between the heritability of USNHL/SSD and the pigmentary abnormality, shedding a new light on the understanding of the molecular basis of heritable USNHL/SSD. In case of children with congenital SSD, it would be mandatory to rigorously screen pigmentary abnormalities. WS should also be included in the differential diagnosis of children with USNHL/SSD, especially in a familial form.</P>
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Kim Yeon Soo,Lee Su Hyun,Kim Soo-Yeon,Kim Eun Sil,Park Ah Reum,Chang Jung Min,Park Vivian Youngjean,Yoon Jung Hyun,Kang Bong Joo,Yun Bo La,Kim Tae Hee,Ko Eun Sook,Chu A Jung,Kim Jin You,Youn Inyoung,C 대한영상의학회 2024 Korean Journal of Radiology Vol.25 No.1
Objective: To investigate whether reader training improves the performance and agreement of radiologists in interpreting unenhanced breast magnetic resonance imaging (MRI) scans using diffusion-weighted imaging (DWI). Materials and Methods: A study of 96 breasts (35 cancers, 24 benign, and 37 negative) in 48 asymptomatic women was performed between June 2019 and October 2020. High-resolution DWI with b-values of 0, 800, and 1200 sec/mm2 was performed using a 3.0-T system. Sixteen breast radiologists independently reviewed the DWI, apparent diffusion coefficient maps, and T1-weighted MRI scans and recorded the Breast Imaging Reporting and Data System (BI-RADS) category for each breast. After a 2-h training session and a 5-month washout period, they re-evaluated the BI-RADS categories. A BI-RADS category of 4 (lesions with at least two suspicious criteria) or 5 (more than two suspicious criteria) was considered positive. The per-breast diagnostic performance of each reader was compared between the first and second reviews. Inter-reader agreement was evaluated using a multi-rater κ analysis and intraclass correlation coefficient (ICC). Results: Before training, the mean sensitivity, specificity, and accuracy of the 16 readers were 70.7% (95% confidence interval [CI]: 59.4–79.9), 90.8% (95% CI: 85.6–94.2), and 83.5% (95% CI: 78.6–87.4), respectively. After training, significant improvements in specificity (95.2%; 95% CI: 90.8–97.5; P = 0.001) and accuracy (85.9%; 95% CI: 80.9–89.8; P = 0.01) were observed, but no difference in sensitivity (69.8%; 95% CI: 58.1–79.4; P = 0.58) was observed. Regarding inter-diffusionreader agreement, the κ values were 0.57 (95% CI: 0.52–0.63) before training and 0.68 (95% CI: 0.62–0.74) after training, with a difference of 0.11 (95% CI: 0.02–0.18; P = 0.01). The ICC was 0.73 (95% CI: 0.69–0.74) before training and 0.79 (95% CI: 0.76–0.80) after training (P = 0.002). Conclusion: Brief reader training improved the performance and agreement of interpretations by breast radiologists using unenhanced MRI with DWI.
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Kim, Ah-Reum,Yoon, Tae-Ung,Kim, Eun-Jung,Yoon, Jung Woon,Kim, Seo-Yul,Yoon, Ji Woong,Hwang, Young Kyu,Chang, Jong-San,Bae, Youn-Sang Elsevier 2018 Chemical engineering journal Vol.331 No.-
<P><B>Abstract</B></P> <P>A novel Cu(I) loading method, which includes the reduction of CuCl<SUB>2</SUB> to CuCl without an external reducing agent and high-temperature calcination, was developed using the redox properties of coordinatively unsaturated Fe(II) sites in MIL-100(Fe). The successful loading of Cu(I) ions and their redox-couple reactions are supported by various methods such as TEM/EDS, XPS, PXRD, and ICP-AES techniques, as well as N<SUB>2</SUB> adsorption isotherms at 77K. Compared to Cu loaded into isostructural MIL-100(Al) devoid of redox active sites, Cu(I)-loaded MIL-100(Fe) exhibits higher C<SUB>3</SUB>H<SUB>6</SUB>/C<SUB>3</SUB>H<SUB>8</SUB> selectivity and superior air stability. This indicates that the Fe(II) sites in MIL-100(Fe) act as antioxidants that protect the resultant Cu(I) species, as well as reducing agents for CuCl<SUB>2</SUB>. Remarkably, in the typical pressure-swing adsorption (PSA) range (1–5bar), the Cu(I)-loaded MIL-100(Fe) exhibits a large C<SUB>3</SUB>H<SUB>6</SUB> working capacity as well as very high C<SUB>3</SUB>H<SUB>6</SUB>/C<SUB>3</SUB>H<SUB>8</SUB> selectivities that are superior to those of the benchmark adsorbents, zeolite-13X and HKUST-1. Moreover, this material is easily regenerated under mild conditions and exhibits good separation performance under dynamic mixed-flow conditions. This facile method for loading Cu(I) can be applied to other adsorbents containing redox-active sites.</P> <P><B>Highlights</B></P> <P> <UL> <LI> A novel Cu(I) loading method was developed by utilizing the redox-active Fe(II) sites in MIL-100(Fe). </LI> <LI> Fe(II) sites in MIL-100(Fe) act as antioxidants that protect the resultant Cu(I) species. </LI> <LI> Cu(0.6)@MIL-100(Fe) is superior to benchmark adsorbents considering both C<SUB>3</SUB>H<SUB>6</SUB> selectivity and working capacity. </LI> <LI> Cu(0.6)@MIL-100(Fe) exhibits good separation and easy regeneration performance under mixed-flow conditions. </LI> </UL> </P> <P><B>Graphical abstract</B></P> <P>[DISPLAY OMISSION]</P>
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Identifying <i>SYNE1</i> ataxia and extending the mutational spectrum in Korea
Kim, Ji Sun,Kim, Ah Reum,Youn, Jinyoung,Lee, Chung,Kim, Nam-Soon,Park, Woong-Yang,Park, Jong Kyu,Kim, Nayoung K.D.,Cho, Jin Whan Elsevier 2019 Parkinsonism & related disorders Vol.58 No.-
<P><B>Abstract</B></P> <P><B>Introduction</B></P> <P>Recent advances in next generation sequencing technologies have uncovered the genetic background of various diseases. The mutations in the <I>SYNE1</I> gene was previously identified as a potential cause of pure cerebellar ataxia. Although autosomal recessive ataxias are slightly more frequent than autosomal dominant forms worldwide, autosomal recessive forms are extremely rare in Korea. In this study, we aimed to identify <I>SYNE1-</I>associated ataxia by whole exome sequencing in a Korean sample, and to review the prevalence of <I>SYNE1</I> in non-French-Canadians.</P> <P><B>Methods</B></P> <P>Patients with suspected cerebellar ataxia who visited movement disorders clinic from March 2014 to December 2017 were clinically screened. After excluding cases with acquired causes and common genetic causes in Korea, including spinocerebellar ataxia and dentatorubral-pallidoluysian atrophy, 63 undiagnosed subjects were screened for <I>SYNE1</I> mutations by next generation sequencing methods.</P> <P><B>Results</B></P> <P>We identified four novel mutations (one splicing, one truncating, and two missense mutations) distributed throughout the <I>SYNE1</I> gene in two patients. The phenotype was mainly pure cerebellar ataxia in both cases. However, axonal neuropathy, mild frontal dysfunction, and autonomic dysfunction were also revealed. The age of disease onset was relatively late and the disease course was only mildly progressive.</P> <P><B>Conclusion</B></P> <P>Our results indicate that <I>SYNE1</I> mutations are not an uncommon cause of recessive ataxia with additional clinical features in the Korean population. The results of this study should alert neurologists to request <I>SYNE1</I> testing to aid the diagnosis of undetermined adult-onset ataxia in Korean patients.</P> <P><B>Highlights</B></P> <P> <UL> <LI> <I>SYNE1</I> mutation is not uncommon outside the French-Canadian founder population. </LI> <LI> We identified 4 novel mutations distributed throughout the <I>SYNE1</I> gene in 2 patients. </LI> <LI> Phenotype was mainly pure ataxia in Korean <I>SYNE1</I> ataxia. </LI> <LI> Axonal neuropathy and mild cognitive impairment was also shown in <I>SYNE1</I> ataxia. </LI> </UL> </P>
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