Bacterial Endophyte Sphingomonas sp. LK11 Produces Gibberellins and IAA and Promotes Tomato Plant Growth

Abdul Latif Khan,Muhammad Waqas,강상모,Ahmed Al-Harrasi,Javid Hussain,Ahmed Al-Rawahi,Salima Al-Khiziri,Ihsan Ullah,Liaqat Ali,정희영,이인중 한국미생물학회 2014 The journal of microbiology Vol.52 No.8

Plant growth promoting endophytic bacteria have been identifiedas potential growth regulators of crops. Endophyticbacterium, Sphingomonas sp. LK11, was isolated from theleaves of Tephrosia apollinea. The pure culture of Sphingomonassp. LK11 was subjected to advance chromatographicand spectroscopic techniques to extract and isolate gibberellins(GAs). Deuterated standards of [17, 17-2H2]-GA4,[17, 17-2H2]-GA9 and [17, 17-2H2]-GA20 were used to quantifythe bacterial GAs. The analysis of the culture broth ofSphingomonas sp. LK11 revealed the existence of physiologicallyactive gibberellins (GA4: 2.97 ± 0.11 ng/ml) and inactiveGA9 (0.98 ± 0.15 ng/ml) and GA20 (2.41 ± 0.23). Theendophyte also produced indole acetic acid (11.23 ± 0.93μM/ml). Tomato plants inoculated with endophytic Sphingomonassp. LK11 showed significantly increased growth attributes(shoot length, chlorophyll contents, shoot, and rootdry weights) compared to the control. This indicated thatsuch phyto-hormones-producing strains could help in increasingcrop growth.

Diversity of pollinators and their role in the pollination biology of chickpea, Cicer arietinum L. (Fabaceae)

Abdul Latif,Saeed Ahmad Malik,Shafqat Saeed,Naeem Iqbal,Qamar Saeed,Khalid Ali Khan,Chen Ting,Hamed A. Ghramh 한국응용곤충학회 2019 Journal of Asia-Pacific Entomology Vol.22 No.2

Chickpea (Cicer arietinum L.) is an important source of food for people worldwide. In the current study, we studied its pollination biology with special reference to floral visitors along with their visitation rate, frequency and pollen load during 2012 and 2013. We also explored the effect of floral visitors on the capsule weight, seed weight, seed numbers and seed germination. Results revealed three bees, two wasps, five flies, one moth and three butterfly species on the flowers of chickpea. Apis dorsata, A. florea, Amegilla sp. and Eristalinus aeneus were the major species with 434–474, 223–311, 69–74 and 81–136 individuals, respectively in both years. Floral visitors differed significantly in term of visitation frequency with A. florea as the most frequent visitor (9.13–9.86 visits/flower/5 min) followed by E. aeneus (5.43–5.58 visits/flower/5 min) and A. dorsata (1.72–2.31 visits/ flower/5 min) in both years. Similarly, A. florea had statistically highest visitation rate (16.85–19.99 flowers visited/min) followed by E. aeneus (9.73–10.68 flowers visited/min). A. dorsata had significantly higher pollen load on its body (84629–85,104 pollen grains) followed by A. florea (64940–65,135 pollen grains) and Amegilla sp. (64020–65,120 pollen grains). The open-pollinated flowers had significantly higher capsule weight (0.27 ± 0.01 g), seed weight (0.18 ± 0.01 g), seed numbers (1.67 ± 0.07 seeds) and seed germination (95 ± 1.38%) as compared to flowers deprived of pollinators in cages. The results suggested A. florea, A. dorsata and E. aeneus could be effective pollinators of chickpea. Hence these three species can be properly utilized on commercial scale to increase crop yield.

Pollination biology of Callistemon viminalis (Sol. Ex Gaertn.) G. Don (Myrtaceae), Punjab, Pakistan

Abdul Latif,Naeem Iqbal,Muhammad Ejaz,Saeed Ahmad Malik,Allah Bakhsh Gulshan,Abid Mahmood Alvi,Khaliq Dad 한국응용곤충학회 2016 Journal of Asia-Pacific Entomology Vol.19 No.2

A research was conducted to find out the floral traits and pollinator's community of bottle brush (Callistemon viminalis: Myrtaceae). Total numbers of pollen grains, pollen grain viability, stigma receptivity, nectar volume and nectar concentration, numbers of ovules and pollen/ovule ratio were recorded. The pollinators' abundance, visitation rate and frequency were also observed. In field experiment, capsule weight, seed setting and seed numbers in open and caged flowers were also evaluated. The results revealed a total of 128,139 pollen grains/ flower. Among total pollen grains, viable pollen grains were 84.3% and non-viable were 15.69%. There were 275 ovules/flower and pollen ovule ratio was 472.50. Stigma receptivity was decreased with the age of the flowers and stigma remained receptive for about 6 days. The nectar volume and nectar concentration were 13 μL and 29%, respectively. The flowers were visited by nine Hymenopteran, four Lepidopteran, one Dipteran and one bird species. Among all pollinators, bees represented the most abundant species (1290) and showed the highest visitation rate (3.6–13.8 numbers of flowers/min) and visitation frequency (0.22–1.92 individuals/ branch/5 min). Open-pollinated flowers showed statistically higher capsule weight (0.0824 ± 0.001), seed setting (0.052 ± 0.0001) and seed numbers (242.4 ± 2.87) as compared to flowers in cages.

Hollow fiber (HF) membrane fabrication: A review on the effects of solution spinning conditions on morphology and performance

ABDUL LATIF AHMAD,Tunmise A. Otitoju,Boon S. Ooi 한국공업화학회 2019 Journal of Industrial and Engineering Chemistry Vol.70 No.-

HF spinning is a potential configuration in membrane technology due to its self-supporting propertiesand high productivity per unit volume. The fabrication of HF membrane is affected by many parametersand it is important to explore the effect of these parameters on membrane properties. The amount ofresearches on traveling distance are minimal and inadequate to conclude their influence onfiberproperties. The draw ratio has always been neglected (often taken as 1), cannot in most cases equal to 1 asit is influenced by take-up speed, dopeflowrate and solution. Future insights on the above mentionedparameters are important.

Production of Gibberellins by Aspergillus fumigatus sp.LH02 and Its Propitious Endophytic Association to Increase Isoflavone Contents Under High Salt Stress and Plant Growth Promotion in Soybean

Abdul Latif Khan,Muhammad Hamayun,Yoon-Ha Kim,Sang-Mo Kang,Joon-He Lee,In-Jung Lee 한국작물학회 2010 한국작물학회 학술발표대회 논문집 Vol.2010 No.04

Endophytic Aspergillus Fumigatus sp. LH02, isolated from the roots of soybean and indentified through phylogenetic analysis of 18S rDNA sequence, was found stimulatory to the growth of Waito-C rice seedlings among 13 other endophytic strains screened. In growth hormone analysis, pure culture of fungal isolate produced three gibberellins viz GA4, GA9 and GA12. To know the role and effect on soybean in endophytic association (EA) and non-endophytic association (NEA) under salt stress (70 and 140mM), experiment was carried out at growth chamber using complete randomized block design. The results elaborated that the EA has significantly increased the shoot length, shoot and root fresh and dry weights, chlorophyll content, photosynthesis rate and leaf area of the plants with and without salt stress compared to control. Field emission scanning electron microscope analysis of root's surface showed that EA plants were extensively colnized by Aspergillus fumigatus LH02 under high salanity. In phytohormonal anlysis, ABA contents in EA and NEA were almost similar. Howere, when plants were exposed th salt stress, ABA levels were found significantly low in EA compared to NEA plant. In isoflavone content analysis, aglycones, malonyl, acetyl and glucosides isoflavones (total 11 compounds) were analyzed for each treatment through extensive HPLC andalysis. The quantities of isoflavone were found higher in EA plant's leaves with and without salt stress compared to NEA plant leaves. In conclusion, such endophytic association can be beneficial to explore ways to improve crop production under stressful conditions.

Vocal Symptoms and Acoustic Findings in Menopausal Women in Comparison to Pre-menopause Women with Body Mass Index as a Confounding Variable

( Abdul-latif Hamdan ),( Georges Ziade ),( Georges Tabet ),( Rachel Btaiche ),( Ghina Fakhri ),( Firas Yatim ),( Doja Sarieddine ),( Muhieddine Seoud ) 대한폐경학회 2017 대한폐경학회지 Vol.23 No.2

Objectives: To compare the prevalence of phonatory symptoms in menopausal women compared to pre-menopause women with body mass index (BMI) as a confounding variable. Methods: A total of 69 women, 34 menopausal and 35 pre-menopausal were invited to participate in this study. Demographic variables included age, smoking, and BMI. All subjects were asked about the presence hoarseness, loss of high or low frequencies, pitch breaks, throat clearing, dryness in the throat and vocal fatigue. Acoustic analysis was performed and the Voice Handicap Index (VHI)-10 was filled by all the subjects. Results: There was a significantly higher prevalence of throat clearing and dryness in the menopausal group compared to the pre-menopause group with a P value of 0.035 and 0.032 respectively. When BMI was taken into account, there was no statistically significant difference in the prevalence of any of the phonatory symptoms in menopausal women with high BMI and pre-menopause. There was no statistically significant difference in the means of any of the acoustic parameters between the menopausal group and pre-menopausal group. There was no significant difference in the mean VHI-10 between the menopause group and the pre-menopause (P = 0.652). Conclusions: The results of this investigation substantiate the importance of fat as an alternative source of estrogen which can mask some of the phonatory symptoms. (J Menopausal Med 2017;23:117-123)

Robust Achievable Rate for Multiuser mmWave Massive MIMO Systems

Abdul Latif Sarker,Dong Seog Han 한국통신학회 2018 한국통신학회 학술대회논문집 Vol.2018 No.11

Optimization of PES/ZnO mixed matrix membrane preparation using response surface methodology for humic acid removal

ABDUL LATIF AHMAD,Abdullah Adnan Abdulkarim,Suzylawati Ismail,Ooi Boon Seng 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.3

The application of response surface methodology (RSM) in preparation and optimization of membranes is important in order to reduce the effort and time needed to achieving an optimum performance. RSM was used to develop an optimum polyethersulfone (PES)/ZnO mixed matrix (MM) membrane for humic acid removal. The MMs were synthesized by dispersing various amounts of hydrophilic ZnO nanoparticles (NPs) into a solution containing PES, polyvinylpyrrolidone (PVP) and dimethylacetamide (DMAc). Flat sheet MM membranes were prepared via the phase inversion method using the central composite design (CCD). The effects of four preparation parameters, such as PES, ZnO, PVP weight percentages and solvent evaporation time, were investigated. Pure water flux (PWF), humic acid flux (HAF) and humic acid rejection (HAR) were selected as a model responses. It was shown that PES and PVP were mainly affected on both PWF and HAF. Furthermore, the interaction effect between PES and ZnO-NPs shows a significant effect on PWF, while the quadratic effects of both solvent’s evaporation time and ZnO-NPs weight percentage coupled with the interaction effect between PES and PVP weight percentage shows the most significant parameters that affects HAR. The optimization method was subjected to maximize all of the PWF, HAF and HAR. It was also determined that the optimized membrane can be synthesized from a solution containing 17.25 wt% PES, 3.62 wt% ZnO and 3.75 wt% PVP with 15 s of solvent evaporation time. The optimum values of PWF, HAF and HAR were 222.3 (L/m2 h), 94.7 (L/m2 h), and 96.34%, respectively. Thus, it can be concluded that the CCD technique is capable of optimizing PES-ZnO membrane performance.

Process modeling and optimization studies of high pressure membrane separation of CO2 from natural gas

ABDUL LATIF AHMAD,Jimoh Kayode Adewole 한국화학공학회 2016 Korean Journal of Chemical Engineering Vol.33 No.10

Process design and optimization methodology for high pressure membrane removal of CO2 from natural gas was developed. An approximate model based on plasticization pressure and permeability parameters at plasticization was proposed for quick evaluation of membrane materials for the high pressure operation. The model was derived by applying the partial immobilization assumption to the fundamental model of solution - diffusion mechanism along with a modified upper-bound curve. About ninety membranes obtained from literature were used to illustrate this methodology. The best three were selected for detailed process modeling and optimization. Process optimization was achieved via non-linear programming constraint optimization model. Gas processing cost was used as the objective function, while plasticization pressure and the CO2 concentration in the feed were used as the constraints. Membrane of 6FDA-durene had the lowest annual gas processing cost while 6FDA-DAM : DABA 2 : 1 had the highest optimum product purity.

The Effect of Broken Orange Pecco tea on caries activity of mutans streptococcal level in dental plaque

Abdul Latif,S. Mangundjaja 대한예방치과학회 2009 International Journal of Clinical Preventive Denti Vol.5 No.4

Objectives: Tea is a traditional drink that is beneficial to health. One of its benefits is its capability to prevent dental caries because it has active substance polyphenol. The aim of the study is to determine the effect of Broken Orange Pecco(BOP) tea on caries activity level of mutans streptococci in plaque after consuming a glass of tea twice daily for one month. Materials and methods: Before enrolled in the study, informed consents were received from their parents. The subject of this study were 30 ,twelve-year old schoolchildren which lived in tea plantation of Malabar, West of Java Indonesia selected for this purpose. 30 subjects conducted two times of treatment as follows: first they were treated as control group by not drinking Broken Orange Pecco tea for one month, and then they were treated as treatment group by drinking a glass of Broken Orange Pecco tea twice daily for one month. Plaque samples were taken with a sterile toothpick along the buccal gingival margin of upper lower and the fissures of the molars. The tips were cut off and put directly into a small tube of Cariostat (Tzu Shimono et al, 1986) and incubated in anaerobic jar. Data obtained were analyzed descriptively and "t- test. Results: The data showed that there is no significant difference of mutans streptococci in group not treated by Broken Orange Pecco tea. However, a significant difference (p=0.000) was found as results in group treated with Broken Orange Pecco tea. Conclusion: Therefore it could be concluded that drinking Broken Orange Pecco tea is effective in inhibiting caries activity levels of mutans streptococci in plague and could be useful for controlling dental caries. Objectives: Tea is a traditional drink that is beneficial to health. One of its benefits is its capability to prevent dental caries because it has active substance polyphenol. The aim of the study is to determine the effect of Broken Orange Pecco(BOP) tea on caries activity level of mutans streptococci in plaque after consuming a glass of tea twice daily for one month. Materials and methods: Before enrolled in the study, informed consents were received from their parents. The subject of this study were 30 ,twelve-year old schoolchildren which lived in tea plantation of Malabar, West of Java Indonesia selected for this purpose. 30 subjects conducted two times of treatment as follows: first they were treated as control group by not drinking Broken Orange Pecco tea for one month, and then they were treated as treatment group by drinking a glass of Broken Orange Pecco tea twice daily for one month. Plaque samples were taken with a sterile toothpick along the buccal gingival margin of upper lower and the fissures of the molars. The tips were cut off and put directly into a small tube of Cariostat (Tzu Shimono et al, 1986) and incubated in anaerobic jar. Data obtained were analyzed descriptively and "t- test. Results: The data showed that there is no significant difference of mutans streptococci in group not treated by Broken Orange Pecco tea. However, a significant difference (p=0.000) was found as results in group treated with Broken Orange Pecco tea. Conclusion: Therefore it could be concluded that drinking Broken Orange Pecco tea is effective in inhibiting caries activity levels of mutans streptococci in plague and could be useful for controlling dental caries.