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Kinetic 방법을 이용한 Ceruloplasmin의측정법과 성적의 검토
황의대 ( Eui Dai Whang ),김태운 ( Tae Woon Kim ),박일진 ( Ill Jin Park ) 대한임상검사과학회 1985 대한임상검사과학회지(KJCLS) Vol.17 No.1
Ceruloplasmin is an enzyme which catalyzes the oxidation of P-phenylenediamine by oxygen to purple-colored compound. This enzyme is a blue-colored metalloprotein with an absorbance maximum at 605nm and specific absorptiyity of 0.068×liter×g-1×cm-1 The protein migrates electrophoretically as analpha 2 globulin and contains 0.355 percent copper. Ceruloplasmin is a glycoprotein, containing 8 to 10 sialic acid residues per mol, for which relative molecular masses varying from 132, 000 to 162, 000 have been reported. Before the enzyme properties of ceruloplasmin were discovered by Holmberg and Laurell, the protein was of interest as one of the "acute phase reaction" proteins present in serum. Ceruloplasmin is of most interest clinically because of its relation to Wilson``s disease. Ceruloplasmin level in serum is a significant sex difference, with the lever in women some 20 percent higher than that in man. The liver is the source of the protein enzyme present in plasma. The level at birth is about 20 percent of that seen in adults. We attempt to introduce and establish more rapid completion of ceruloplasmin determination based on kinetic method using a Gilford 203-S automatic chemistry analyzer.
Centrifugal analyer를 이용한 혈청중 Complement C3 C4 측정법의 검토
홍순동 ( Soon Dong Hong ),황의대 ( Eui Dae Whang ),김태운 ( Tae Woon Kim ),박알진 ( Il Jin Park ),박애자 ( Ae Ja Park ),정낙승 ( Nak Seung Jung ) 대한임상검사과학회 1984 대한임상검사과학회지(KJCLS) Vol.16 No.1
The complement acts as a part of immune system to protect the individual from microbial infection by mediating a variety biologic reaction; opsonization, chemotaxis of leukocytes, increased vascular permeability, and cytolysis of target organisms. The complement system is consisted about 18 proteins, among them, The C3 and C4 are the highest concentrates in serum. These proteins are said to be in "classic pathway" or "alternative pathway" names. Activation of the complement proteins result in loss of their precursors, native activities and, or their accelerated clearence from p1asma. Progressing hypercatabolism without compensation by increased synthesis of complements, the determination of depressed levels of them may provide the evidence of the comp1ement system activation. For the evaluation of the patient with autoimmune disease such as autoimmune hemolytic anemia, SLE, rheumatoid arihritis and poststreptococcal glomerulonephritis, the determination of C3 and C4 concentration are both of the most informative method. Low concentration of C4 indicated that classic pathway activation has occurred, since C4 is extremely sensitive to C1 Depressed level of C3 suggests that rather intense activation of ether pathway. Decreased C3 associated with normal C4 levels indicate exclusive activation of alternative pathway. During the past decade, a number of invastigators have developed the techniques for measurement of complement. Since 1929, Michael Heidleberger has developed immunoprecipitation analysis of complement. And also Boyden et al (1947) demonstrated turbidimetric absorbance using the basis of antigen quantitation. The radial immunodiffusion (RID) technique has proved to be one of the most useful methods in clinical laboratories during the past few years, but it require rather long processes from 18 to 48 hrs in order to complete precipitation ring formation. So we attempt to introduce and establish more rapid completion of complement quantitation based on turbidimetric technique using a centrifugal analyzer.
조광현,우종수,황윤호,이양행,박철호,김종성,김철호,이경순,조영일,박태인 인제대학교 1985 仁濟醫學 Vol.6 No.4
A successful treatment of infants and children with congenital heart defects depends upon several factors: diagnosis must be accurate, the operative technique, including techniques of perfusion and myocardial preservation, must be suited to the needs of patients and intensive cares, including accurate anesthesia, must be applied through all stages of investigation and treatment, i.e. before, during and after the operation. Recently we performed a open heart surgery successfully for a six-years old girl who had suffered from frequent upper respiratory tract infection and mild to moderate exertional dyspnea with congenital heart defects (ventricular septal defect, Kirklin type II, with patent foramen ovale). The operation and postoperative course were very smooth. And now we report this with review of literatures.