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오지은(Ji Eun Oh),박상우(Sang Woo Park),임춘수(Chun Soo Lim),김연수(Yon Su Kim),안규리(Cu Rie Ahn),한진석(Jin Suk Han),김성권(Shung Gwon Kim),이정상(Jung Sang Lee) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.3
목 적: 일반적으로 본인이 건강하다고 알고 있는, 소위 ‘정상’ 성인에서 우연히 발견된 단백뇨의 임상적 의미를 알아보고자 하였다. 방 법: 1995년 5월 1일부터 2000년 2월 11일까지 약 5년간 서울대학교병원 건강증진센터에서 검진을 받은 성인 중 혈압약을 복용하지 않고, 공복시 혈당이 126 mg/dL 미만인 22,595명에서 실시한 집단 검진 결과를 단면적 연구로 분석하였다. 결 과 : 총 대상자 22,595명(남자 11,737명, 여자 10,858명) 중 dipstick 요검사에서‘±’이상의 단백뇨의 유병율은 남자 6.7%, 여자 3.6%였다. 단백뇨의 위험 인자에 대한 단순 연관성 분석을 시행하였을 때, 나이, 성별, 몸무게, 수축기 혈압, 이완기 혈압, 공복시 혈당, BUN, 혈청 크레아티닌, 총 콜레스테롤과 흡연 여부가 임상적으로 중요한 위험인자로 나타났다. 단백뇨와 혈압의 관계를 살펴보았을 때 dipstick 요검사에 따른 단백뇨의 정도가 증가함에 따라 수축기와 이완기 혈압이 증가하였다. Cockroft 공식에 의한 크레아티닌 청소율은 단백뇨의 정도가 증가함에 따라‘++’이상에서 유의한 감소를 나타내었다. 혈압의 정도를 제 6차 Joint National Committee의 기준으로 구분하여 혈압의 증가에 따른 단백뇨의 확률을 살펴보았을 때, 전체적으로 혈압이 증가함에 따라 단백뇨의 확률도 증가하였으며, 이러한 증가 추세는 정상 혈압과 고-정상 혈압사이, 고-정상 혈압과 1단계 고혈압사이, 2단계 고혈압과 3단계 고혈압사이에서 유의한 증가를 보였다. 혈압과 크레아티닌 청소율과의 상관관계를 살펴보았을 때, 전체적으로 혈압이 증가하면 크레아티닌 청소율이 감소하는 양상을 나타내었다. 이를 단백뇨가 있는 군과 없는 군으로 나누어 분석하였을 때, 혈압이 고-정상 혈압과 1단계 고혈압에서 단백뇨가 있는 군에서 단백뇨가 없는 군에 비해 크레아티닌 청소율의 유의한 감소를 나타내었다. 결 론: 이상에서, 소위‘정상’성인에서 우연히 발견된 단백뇨라도 일반적으로 알려진 것처럼 양성의 경과를 밟는 것만은 아니며, 유의한 혈압의 증가와 신기능의 감소를 동반할 수 있음을 시사하는 결과이다. Purpose : This study was implemented to investigate the prevalence of proteinuria and its combined morbidity in apparently normal adults. Methods : We examined the mass screening data of Health Promotion Center in Seoul National University Hospital from May 1, 1995 to February 11, 2000. The random urine samples of all screenees were examined by dipstick test. Among them 22,595 adults(men 11,737 and women 10,858) who didn`t take anti- hypertensive medication and whose fasting blood sugar <126 ㎎/dL were included in this analysis. Results : The prevalence of proteinuria was 6.7% in men and 3.6% in women. Risk factors for proteinuria by simple correlation analysis were age, sex, body weight, systolic blood pressure, diastolic blood pressure, fasting blood sugar, blood urea nitrogen, serum creatinine, total cholesterol and smoking. As the degree of proteinuria increased, the systolic and diastolic blood pressures also increased significantly and creatinine clearance significantly decreased above the‘++’level of proteinuria. Probability of proteinuria was calculated at each blood pressure level graded by JNC Ⅵ. With the increase of the level of blood pressure, the probability of proteinuria increased significantly between normal and high normal, high normal and hypertension1, and hypertension 2 and hypertension 3 level. Creatinine clearance and blood pressure level showed negative correlation. When total screenees were divided to proteinuria and no proteinuria groups, proteinuria group showed significant decrease of creatinine clearance in high normal and hypertension 1 level. Conclusion : Our results suggest that proteinuria in the apparently normal adults is not a benign condition, and it can be accompanied by significantly increased blood pressures and decreased renal function.
김지영 ( Ji Young Kim ),박재선 ( Jae Seon Park ),남용재 ( Yong Jae Nam ),유은진 ( Eun Jin Yoo ),이원석 ( Won Seok Lee ),최성헌 ( Sung Hun Choe ),한진석 ( Jin Seok Han ),최종우 ( Jong Woo Choi ) 한국환경분석학회 2012 환경분석과 독성보건 Vol.15 No.2
Analytical conditions of amino acids in ground-water by using UFLC-MS/MS(ESI) were optimized for prompt and accurate analysis. Under the Edmen`s method, derivatization of amino acids with PITC (phenylisothiocyanate) was performed and chromatographic separation was achieved on ODS (octadodesylsulfonate)- column with 3,400 psi. Within 10 minutes (6 min for MS collection), 21 types of amino acids were simultaneously analyzed by UFLC-MS/MS (ESI) after the selection of qualitative and quantitative ions. This method was precise with percent relative standard deviation of 2.6~13.8 % on 7 replicate analysis and accurate with percent recovery of 81.6~115.3 %. Confidence interval (CI, 3.53~4.25 mg/L). coefficient of determination (r2, 0.970562 (TRP)~0.999329 (VAL)), limits of detection (0.29(PRO)~1.88(ASN) mg/L (mean. 0.91 mg/L)), and limits of quantification (0.99(PRO)~6.28(ASN) mg/L (mean, 3.03 mg/L)) were obtained and proven to be a reliable method. Derivatives was relatively stable when it occurred for more than three hours under 45oC based on optimum tests. Also, it was notable that m/z 164.15 ion, reagent of derivatization under MS/MS, was identified with phenylalanine in the same time frame, indicating excessive quantity. However, when the quantitative ion was changed from m/z 164.15 to m/z 221.10, relevant result was achieved.
토양분야 환경오염공정시험기준과 KS ISO규격의 일원화에 관한 연구
김지인 ( Ji In Kim ),김보경 ( Bo Kyong Kim ),김정화 ( Joung Hwa Kim ),이군택 ( Goon Teak Lee ),이상욱 ( Sang Uk Lee ),안문성 ( Mun Seong Ahn ),임태숙 ( Tae Sook Lim ),한진석 ( Jin Seok Han ),이원석 ( Won Seok Lee ) 한국환경분석학회 2012 환경분석과 독성보건 Vol.15 No.3
Korea has two type of the environmental official test methods, environmental standards enacted by ministry of environment (ES) and Korean industrial standard enacted by ministry of knowledge and Economy (KS), which causes the confusion of users, The main purpose of this study is to compare ES with KS and to make solutions to unify two types of standards. futhermore, We found the ways to improve ES and KS considering many countries aligned domestic standards with international standards. ES for soil quality consisted of sixty official test methods. We focused on thirty official test methods in ES except for the introduction, sampling methods and leak test methods and compared them with the corresponding KS just translated from ISO standard without any technical changes. By reviews and comparative tests between ES and KS we classified them into “No corresponding standards”, “Pre-unification completed”, “Pre-unification impossible”, “Unification completed” and “Unification impossible”. There were eighteen standards possible to unify, six standards impossible to unify and six standards corresponding no KS. We suggested that ESs for CN, phenols and Cr6+ were needed to adopt parts of the procedures in KS for improving recoveries and reducing the pre-treatment time and labor. We also found that both standards had to include detailed information about wavelength to analyze metals for user`s convenience.
Monocyte Chemoattractant Protein - 1 ( MCP-1 ) 조절부위에 따른 유전자다형성이 MCP-1 발현 및 낭창성 신염에 미치는 영향
오윤규(Yoon Kyu Oh),한진석(Jin Suk Han),안규리(Cu Rie Ahn),이정은(Joung Eun Lee),김연수(Yon Su Kim),김성권(Suhng Gwon Kim),이정상(Jung Sang Lee),김현리(Hyun Lee Kim),양승희(Seung Hee Yang),오지은(Ji Eun Oh),윤형진(Hyung Jin Yoon) 대한신장학회 2002 Kidney Research and Clinical Practice Vol.21 No.1
목 적 : MCP - 1 조절부위에 대한 유전자다형성이 MCP - 1 발현과 낭창성 신염의 발병, 진행에 대한 영향을 알아보고자 본 연구를 시행하였다. 방 법 : 전신성 홍반성 낭창(SLE) 환자 49명, 대조군 46명에서 유전자다형성을 결정 후, 각 유전자형에 따라 luciferase assay로 단백질 발현정도를 비교하였다. 정상 대조군 6명의 유전자형에 따라 말초혈액 단핵세포를 자극 후 배양 상청액의 MCP - 1 농도를 측정하였고 SLE 89명, 정상 대조군 21명의 혈중 MCP - 1 농도를 측정, 유전자형에 따라 신 침범과의 연관성을 조사하였다. 결 과 : SLE 환자에서 MCP- 1 조절부위(-2518 position :A- >G) 유전자다형성은 환자군(n=49)에서 각각 AA 10명(20%), GA 21명(42%), GG 18명(38%)으로 정상대조군(n=46)의 9명(20%), 27명(58%), 46명(22%)과 차이가 없었다. 말초혈액 단핵세포를 자극 후 배양 상청액의 MCP- 1 농도는 AA군이 현저히 증가되었다(p=0.01). 293 T 세포에 AA와 GG 유전자 이입 후 luciferase assay 시행시 GG 유전자 단백발현이 AA의 39%로 단백발현 감소(p=0.014), TNF-α를 처리 후 AA 유전자와 GG군의 construct 모두에서 activity가 증가되었고 특히 AA 유전자 단백발현이 GG 보다 3배 이상 증가되었다. SLE 환자군의 혈중 MCP- 1 농도는 418.17±935.30 pg/mL로 정상 대조군(127.78±114.53 pg/mL)에 비해 높았으나(p=0.047), 환자군의 혈중 MCP- 1 농도와 요단백량의 연관성은 없었다. 낭창성 신염으로 진단된 환자와 다른 대조군들 사이의 혈중 MCP- 1 농도를 비교하였을 때 의미있는 차이는 없었으나, AA homozygote군의 낭창성 신염 환자들에서 혈중 MCP- 1 농도가 신염이 없는 AA군에 비해 증가되었다(p=0.014). 결 론 : MCP - 1 유전자다형성이 단백발현의 차이를 낳고 낭창성 신염 진행에 중요한 역할을 하며 신질환 진행의 예측과 함께 적절한 치료방침을 세우는데 유전자다형성이 중요한 요소로 고려되어야 한다. Background : Monocyte chemoattractant protein-1(MCP-1) plays an important role in progression of lupus nephritis.(LN) The genetic polymorphism in the regulatory region would influence clinical manifestations by controlling serum levels of MCP-1. Methods : We determined the genotypes of the MCP-1 gene, the secretion of MCP-1 by pheripheral blood monocytes (PBMCs) and transcription activity according to polymorphism on ELISA and luciferase assay. We also correlated serum MCP-1 level with proteinuria according to the genotypes to evaluate the clinical implication of genetic polymorphism in LN. Results : 10 patients with SLE (20%) were AA homozygous, 21(42%) GA heterozygous, and 18(38%) GG homozygous, which w as similar with normal controls[AA 9(20%), GA 27(58%), GG 46(22%)](n=46). By in-vitro stimulation of PBMCs using Phytohemagglutinin, differential expression of MCP-1 appeared according to the genotypes at -2518 position; PBMCs from AA homozygotes 22.37±0.07 ng/ mL, GA 6.98±0.72 ng/ mL, GG 5.48±0.22 ng/ mL. In the luciferase assay, the gene construct with G at -2518 site showed decreased activity to 39% of that showed by A gene construct . In addition, After cells were treated with TNF-α (10 ng/mL), the transcription activity of A gene construct w as approximately 3 fold greater than that of G gene construct. Levels of serum MCP-1 were significantly higher in patients with SLE (n=89) than normal controls (n=21)(418.17±935.30 pg/mL vs. 127.78±114.53 pg/mL, respectively; p<0.05). In contrast, there were no significant differences in serum MCP-1 levels between patients with LN, patients without LN and normal controls. Also, correlation between serum MCP-1 levels and proteinuria was not found(r=0.191, p>0.05). But, in patients with LN, levels of serum MCP-1 were significant higher in patients with AA genotype than those of GA genotyes and GG genotypes (p<0.01). Conclusion : MCP-1 gene polymorphism at regulatory region may be a considerable marker for LN and may modulate the level of protein expression. Our study could make it possible to screen high risk individuals, thus help us to develop a practical application of the molecular findings in clinical practice.