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한규삼,오병관,Han Kyu-Sam,Oh Byoung-Kwan 대한수의사회 1984 대한수의사회지 Vol.20 No.5
The authors made a survey infestation rate of endoparasites in wild .animals ( deer 68 heads and wild boar 88 heads) at Chon-Buk province. The results are summarized as follows; 1. Infection rate of species in deer Fasciola hepatica $22.8\%$ Oe
한규삼,Han Kyu-sam 대한수의사회 1986 대한수의사회지 Vol.22 No.10
A total of 1.252 quarters milk sample (998 from 267 cows of 37 herds and 254 of received from dairyfarms) in Chunbuk province were classified as clinical and subclinical group by CMT and RBU test. Of these positive sample, the mastitic agents were isolate
뱀장어에서 분리한 불정형 Aeromonas salmonicida EL-I의 일부 특성과 병원성에 관한 연구
한규삼,최인열,배정준,신성일,김영진,김영길,이근광 한국동물위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.1
A partial characteristics and pathogenicity of Aeromonas salmonicida strain isolated from diseased cultured eel were studied. On the biochemicial characteristics of the isolate strain was classified into atypical Aeromonas salmonicida : the characteristics of the isolate strain differed from those of the subspecies of Achromogenes, masoucida and salmonicida. It strain was named atypical Aeromonas salmonicida EL-1 and it grew at optimal pH 7.5 and l% Nacl. In the pathogenicity test, at one to two days post infection, the motality of artificially infected eels with $1{\times}10^8$ and $1.0{\times}10^7$ cfu/fish group was 100%, and at three days pos-infection, in the $1.0{\times}10^6$ /cfu /fish infected group, the epizootic haemorrhagic ulcer was developed, and haemorragic red sports were formed around abdomen and fins and its died (40%). Total cell protein peptide bands of the A. salmonicida EL-1 were presented betweeon 11.8~102 Kd in molecular weight by the electrophoretic analysis and appeared 28 bands in all. Drug sensitivity of atypical A. salmonicida EL-1 strain was sensitivity to chlorampenicol, gentamicin, kanamycin, streptomycin and sulfamethoxazole /trimethoprim and intermidiate to ampiclin and neomycin resistant to cephalotin, erythromycin, lincomycin and tetracycline.
韓國産메기(Silurus asotus)의 疾病에 關한 연구 Aeromonas hydrophila에 의한 皮膚 潰瘍病
한규삼(Kyu-Sam Han),최인열(In-Yeol Choi),배정준(Joung-Joon Bae),김영길(Young-Gill Kim),이근광(Keun-Kwang Lee),김영진(Young-Jin Kim) 군산대학교 수산과학연구소 1994 군산대학교 수산과학연구소 연구논문집 Vol.2 No.-
The Aeromonas strains were isolated from diseased cultured catfsh(Silurus asotous) in the ponds near by Gochang of Chunbuk. The present isolates were identified as A. hydrophila based on their biological aud biochemical characteristics. The isolates of A. Hydrophila were named GC-l, GC-2, GC-3, GC-4 and GC-5. Five strains were grew optimally at temperatures 35℃. pH 7.5 and in 0.5 to 1% NaCㅣ, This bacterium(GC-l) was injected into health catfish in order to prove the causative agent of ascites and haemorrhagic ulcers. The symptoms in catfish infected by this challenge method were observed to be very similar to the symptoms of a natural infection, but controls did not show any abnormal symptoms during the experimental period. At 24 post-injection, the red spot developed around the injection site and the haemorrhagic ulcers was extended near the gill and ventral fin. Five strains were tested for drug sensitivity by plate method. All strains were sensitivity to gentamicin and resistant to penicillin, streptomycin and tetracycline.
담수어류에서 Argulus japonicus의 실험감염에 관한 연구
한규삼 ( Kyu Sam Han ),정영미 ( Young Mee Jung ),박태욱 ( Tae Wook Park ),임채웅 ( Chae Woong Lim ),송희종 ( Hee Jong Song ),도홍기 ( Hong Ki Do ) 한국가축위생학회 1999 韓國家畜衛生學會誌 Vol.21 No.4
Argulus spp, a small group of parasitic curstaceans called Branchiura, is particularly important in freshwater acquaculture. We investigated the infection of Argulus to several different species of fishes and therapy effect of masoten. Argulus japonicus was collected from Okjeong lake, Chonbuk province and experimen-tally infected to freshwater fishes from July to August 1998. The results were as follows; 1. The shape of a Agulus is an oval and dorsoventrally flattened, with first maxillae and second maxillae, and four pairs of legs. 2. The size of the adult stage of A japonicus was 3.2-5.0×2.3-2.9mm in female and 1.5-3.1×1.6-2.9mm in male. 3. In the trial of experimental infection with A japonicus to non-scaled fishes, Parasilurus asotus and Ictalurus punctalus, were infected and some of them died after severe infection. However, A japonica was not infected at all. 4. Masoten treatment for A japonicus was effective at 0.2 ppm once a day for 3 days and parasites on the surface of fishes disappeared after five days.
한국산(韓國産)메기(Silurus asotus)의 질병(疾病)에 관(關)한 연구 Aeromonas hydrophila에 의한 피부(皮膚) 궤양병(潰瘍病)
한규삼 ( Kyu Sam Han ),최인렬 ( In Yeol Choi ),배정준 ( Joung Joon Bae ),김영길 ( Young Gill Kim ),이근광 ( Keun Kwang Lee ),김영진 ( Young Jin Kim ) 한국가축위생학회 1993 韓國家畜衛生學會誌 Vol.16 No.2
The Aeromonas strains were isolated from diseased cultured catfsh(Silurus asotous) in the ponds near by Gochang of Chunbuk. The present isolates were identified as A. hydrophila based on their biological and biochemical characteristics. The isolates of A. Hydrophila were named GC-1, GC-2, GC-3, GC-4 and GC-5, Five strains were grew optimally at temperatures 35℃, pH 7.5 and in 0.5 to 1% NaCl, This bacterium(GC-1) was injected into health catfish in order to prove the causative agent of ascites and haemorrhagic ulcers. The symptoms in catfish infected by this challenge method were observed to be very similar to the symptoms of a natural infection, but controls did not show any abnormal symptoms during the experimental period. At 24 post-injection, the red spot developed around the injection site and the haemorrhagic ulcers was extended near the gill and ventral fin. Five strains were tested for drug sensitivity by plate method. All strains were sensitivity to gentamicin and resistant to penicillin, streptomycin and tetracycline.
뱀장어에서 분리한 부정형(不定形) Aeromonas salmonicida EL-1의 일부 특성과 병원성에 관한 연구
한규삼 ( Kyu Sam Han ),최인열 ( In Yeol Choi ),배정준 ( Joung Joon Bae ),신성일 ( Sung Il Shin ),김영진 ( Young Jin Kim ),김영길 ( Young Gill Kim ),이근광 ( Keun Kwang Lee ) 한국가축위생학회 1995 韓國家畜衛生學會誌 Vol.18 No.1
A partial characteristics and pathogenicity of Aeromonas salmonicida strain isolated from diseased cultured eel were studied. On the biochemicial characteristics of the isolate strain was classified into atypical Aeromonas salmonicida: the characteristics of the isolate strain differed from those of the subspecies of Achromogenes, masoucida and salmonicida. It strain was named atypical Aeromonas salmonicida EL-1 and it grew at optimal pH 7.5 and 1% Nacl. In the pathogenicity test, at one to two days post infection, the motality of artificially infected eels with 1×10(8) and 1.0×10(7)cfu/fish group was 100%, and at three days pos-infection, in the 1.0×l0(6)cfu/fish infected group, the epizootic haemorrhagic ulcer was developed, and haemorragic red sports were formed around abdomen and fins and its died(40%). Total cell protein peptide bands of the A. salmonicida EL-1 were presented betweeon 11.8~102 Kd in molecular weight by the electrophoretic analysis and appeared 28 bands in all. Drug sensitivity of atypical A. salmonicida EL-1 strain was sensitivity to chlorampenicol, gentamicin, kanamycin, streptomycin and sulfamethoxazole/trimethoprim and intermidiate to ampiclin and neomycin resistant to cephalotin, erythromycin, lincomycin and tetracycline.