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RISS 인기검색어
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- 저자 : 최은아(E. A. Choi) (검색결과 3 건)
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황체화된 인간 과립세포에서 Apoptosis 관련 유전자인 bcl-2와 TRPM-2의 발현
이병석,최은아,장경환,김진영,배상욱,박기현,조동제,이국,김재욱,송찬호,Lee, B.S.,Choi, E.A.,Chang, K.H.,Kim, J.Y.,Bae, S.W.,Park, K.H.,Cho, D.J.,Lee, K.,Kim, J.W.,Song, C.H. 대한생식의학회 1997 Clinical and Experimental Reproductive Medicine Vol.24 No.2
Apoptosis, programmed cell death, is posulated to occur in granulosa cells in ovarian follicular atresia. bcl-2 gene serves as protector from apoptosis and, thus, is associated with increased cell survival. TRPM-2 gene expression has been implicated as a trigger of apoptosis in rat prostate, uterus and mammary gland. Our objective was to determine if bcl-2 and TRPM-2 are expressed in luteinized human GC and, therefore, have regulatory functions for apoptosis in GC. Human GC were obtained via oocyte retrival from the infertile patients stimulated with exogeneous gonadotropins while undergoing IVF. GC were isolated from follicular fluid using Percoll gradient centrifugation. The GC were further purified with anti-CD45 magnetic beads to remove contaminating WBC's. RT-PCR were performed to analyze the mRNA expression of bcl-2 and TRPM-2 in the GC. The PCR primers were designed to amplify a 195 bp fragment of bcl-2 and a 174 bp fragment of TRPM-2. The PCR products were electrophoresed on 4% agarose gel. Three separate experiments indicated that both bcl-2 and TRPM-2 are concurrently expressed in human GC. We cultured granulosa cells with FSH (1 ng/ml) for 1 day to investigate the relative changes of TRPM-2 mRNA level with RNAse protection assay. When we cultured GC with serum free medium for 1 day TRPM-2 mRNA level increased with 1.3 fold, however it was decreased 0.64 fold with FSH. Therefore we conclude that bcl-2 and TRPM-2 are concurrently expressed and that the interaction of their products may be involved in GC apoptosis. And TRPM-2 may be regulated with FSH.
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곽예림,권경미,김유리,신지연,엄영아,오가영,윤미혜,윤예림,정자혜,최은아 이화여자대학교 간호과학대학 2010 이화간호학회지 Vol.- No.44
Nowadays, juvenile's sexual-related delinquency is increasing and their immature sexual behavior is on issue with this recent phenomenon. That is the reason people realize offering right sex education for them is important. So, we are going to study for trying to make an improvement in sex education field based on the fact that is practicing now at school. We will reference "소중한 성 바로알기" in this study which is published in 2008 for schoolteacher to compensate some limitations of former education material. This study was done from June 15 to July 20, 2009(Total : 36days) and aimed for surveying senior year students of middle school located in Seoul, S. Korea. We sampled 248 students by random from 8 classes of 4 schools. We analyzed final 209 samples(the other 39 samples were omitted for inappropriate response and return.) using SPSS WIN 12.0. The questionnaire includes 4 parts of relationship and psychosexual, physical growth and sexual health, social environment and sexual equality, and healthy family with marriage and it consists of 31 questions. We made this questionnaire base on the reference as we stated above. From this study we found the part of healthy family with marriage has not enough contents compared with the other 3 parts. And also we could see students don't feel satisfaction with one-off, one-sided sex education, and the education which is performed with only one single method. Therefore we make a judgement that middle school students have their needs toward sex education at school and those are considered as regular education by well-trained education provider, and various means for education such as case study, discussion, or role play. As well we think sex education for middle school students need to offer more about sexual equality and also alert the increasing possibilities of sexual commit in cyber space to adolescent.
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