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미세현미조작된 생쥐배의 발생과 단일할구의 성감별 기술에 관한 연구 2 . 미세조작에 의해 분리된 할구의 염색체 분석조건에 관한 연구
김익수(I . S . Jeon),정범식(B . S . Jung),박수봉(S . B . Park),최광수(K . S . Choi) 한국축산학회 1991 한국축산학회지 Vol.33 No.7
The study was carried out to investigate the optimum condition of C-banding for sexing mouse embryo by chromosome analysis of single blastomere. The sexing rate was 42.9% with the 114 blastocyst embryos which were used C-banding after being hyp-otonic-treated in 1% sodium citrate for 30 minutes. The separated blastomeres for 4-cell embryos were used for C-banding after being hypotonic-treated in 1% sodium citrate for 20-30, 10-15 and 1-2 min., and the sexing rate for different treatment times were 5.5%. 0%, and 0%, respectively. The separated blastomeres from 4-cell embryos were used for C-banding after being hypotonic-treated in 30% fetal calf serum for 20-30, 10-15 and 1-2 min., and the sexing rate for different treatment times were 18.5%, 0% and 0% respectively.
전익수(I . S . Jeon),김선의(S . E . Kim),정진관(J . K . Jung),정일정(I . C . Cheong),박영식(Y . S . Park),최광수(K . S . Choi) 한국축산학회 1993 한국축산학회지 Vol.35 No.6
The effect of micromanipulation and zona pellucida(ZP) on in vitro viability and development of frozen embryos was investigated for the effective cryopreservation of biopised embryos. The ZP-intact 4-cell embryos and ZP-removed 4-cell embryos were biopised using micromanipulator and micropipette, respectively. Biopsied embryos were equilibrated in M16 containing 3.5M DMSO and 0.25M sucrose for 1.5 minutes and then were ultrarapidly frozen in LN₂. The frozen embryos were thawed at 37℃ and were cultured in M 16 at 37℃ for 48 hours under an atmosphere of 5% CO₂ and 100% humidity. The developmental rate of fresh and frozen embryos was evaluated as percentage of embryo development from 4 cell stage to blastocyst. The results were summarized as: 1) the survival rate of 4-cell embryos ultrarapidly frozen and thawed was 73%. The development rate of survived embryos after freezing was 92%; 2) the survival rate of micromanipulated-embryos ultrarapidly frozen and thawed was 79?. The development rate of survived embryos after freezing was 59%; 3) the developmental rate of the cultured fresh ZP-intact embryos was significantly higher than those of the fresh ZP-removed embryos and fresh ZP-removed biopsied embryos (p$lt;.05); and 4) the developmental rate of the cultured frozen ZP-intact embryos was significantly higher than those of the frozen ZP-removed embryos and frozen ZP-removed biopsied embryos(p$lt;.05).
돼지의 혈청 Insulin-like Growth Factor-I과 산자수간의 연관성 연구
양성호,서동삼,박희복,김기동,강창원,최광수,박성수,홍기창,고용,Yang, S.H.,Seo, D.S.,Park, H.B.,Kim, K.D.,Kang, C.W.,Choi, K.S.,Park, S.S.,Hong, K.C.,Ko, Y. 한국동물번식학회 1999 Reproductive & developmental biology Vol.23 No.3
The litter size has been the primary interest of economic traits in pig reproduction. It has been recently shown that insulin-like growth factor-Ⅰ(IGF-Ⅰ) plays roles in establishing pregnancy and in supporting fetal growth and development. But, the effect of serum IGF-Ⅰ on litter size has not been studied. Therefore, this study was conducted to relate serum IGF-Ⅰ concentration with porcine litter size and to investigate the possible connection with estrogen receptor(ER) as a candidate gene for the litter size. Sera during day 45 to 105 of pregnancy were collected from two groups showing high and low litter size and serum IGF-Ⅰ concentration was measured by radioimmunoassay (RIA). IGF-Ⅰ levels in both groups decreased gradually as pregnancy stage proceeded but were not significantly different. Secondly, DNA was extracted from blood and PCR-RFLP was utilized to analyze ER genotypes of pigs in each group, which produced three polymorphic patterns. Based on the ER genotypes analyzed, low litter size group showed higher IGF-Ⅰ concentration than high litter size group. Taken together, the results indicate that the serum IGF system was correlated with steroid system but not with the litter size in pigs. Thus, this study implies that porcine litter size could be determined locally at the ovary level.
전익수,최광수,손시환,오노타마오 ( I . S . Jeon,K . S . Choi,S . H . Sohn,T . Ono ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
This study examined the production of chick-quail chimera by transfer of blastoderm cells and investigated the developmental pattern of chick blastoderm cells in the quail subgetminal cavity. To produce the chick-quail chimera, 1,500 to 2,000 cells of chick embryos in the blastodermal stage were microinjected into the subgerminal cavity of quail embryos. The presence of the blastoderm cells of chicks in the subgenninal cavity of quail embryos was detected by Feulgen staining. The developmental pattern of the blastodenn cell division of chicks in the quail subgerminal cavity was examined by chromosomal analysis. When quail embryos which were injected with chick blastoderm cells were cultured in surrogate egg-shells, average hatchabilities were 40.0% for the wild type and 45.8% for the black type. A chick-quail chimera was produced from the black type. After the blastoderm cells of chicken embryos were itgected into the subgeminal cavity of quail embryos, the presence of blastoderm cells of chicken embryos was detected by Feulgen staining in the ectoderm, endoderm, and mesoderm of the subgetminal cavity of quail embryos. When the blastoderm cells of chicken embryos were injected into the subgemunal cavity of quail embryos, the chromosomal analysis revealed that the occurrence of blastoderm cell division of chicken embryos was exxremely rare.
전익수,최광수,손시환,오노타마오 ( I . S . Jeon,K . S . Choi,S . H . Sohn,T . Ono ) 한국축산학회 1997 한국축산학회지 Vol.39 No.1
This study examined the pattern of in vitro development of quail embryos cultured in surrogate egg-shells and investigated an in vitro culture system of quail embryos to provide opportunities for generic manipulation of quails. To investigate die pattern of in viro development of quail embryos in surrogate egg-shells, 2-day-old quail embryos were implanted into surrogate egg-shells, sealed with wrap and cultured at 37.6℃ with relative humidity of 65.5±5%. Quail embryos in the blasfodermal stage were implanted into surrogate quail and chicken egg-shells sequentially, sealed with wrap, and cultured at 37.6℃ with the relative humidity of 65.5±5%. An average length of the third toe of quail embryos cultured in surrogate egg-shells at 15 days of incubation was 12.49㎜, and was 13.03㎜ for the control culture. An average dry weight of quail embryos cultured in surrogate egg-shells at 15 days of incubation was 1.216g, and was 1.042g for the control culture. When blastodermal embryos were cultured in surrogate egg-shells of a chicken, the average hatchabilities were 11.8% for wild type and 24.6% for black type. When two-day-old embryos were cultured in surrogate egg-shells of the chicken, average hatchabilities were 55.6% for wild type and 51.3% for black type, and when blastodermal embryos were cultured in surrogate eggshells of quail from the blastodermal stage to 2-days-old, and thereafter to hatching were recultured in surrogate egg-shells of chickens, average hatchabilities were 36.0% for wild type and 51.9% for black type. The results for our culture system indicated that it would be practical for the production of transgenic birds.
Saanen 종과의 누진교잡에 의한 재래산양 개량시험 제2보 , Saanen 종과 재래산양의 교잡종에 대한 비유능력의 변화
이길왕,최광수,탁태영,설동섭,이기만 ( K . W . Lee,K . S . Choi,T . Y . Tak,D . S . Sul,K . M . Lee ) 한국축산학회 1975 한국축산학회지 Vol.17 No.1
The data from 393 purebred and crossbred goats produced by matings of Korean Native Goats and Saanen, which were investigated at Livestock Experiment Station since 1963, were analyzed by least-square method to investigate changes of milking performance of the Korean Native Goats by grading up with the Saanen. This procedure adjusted the measurement data for surveyed year, parity, mating system and for the number of kid born at one birth. The results obtained are as follows. 1. The milk productions of the Korean Native Goats, the first filial, the second, and the third graded generations were 423.91, 90.97, 288.12, 354.81 and 372.76㎏, respectively. Their milk fat percentages were 4.15, 5.72, 5.37, 4.30 and 4.24, respectively. Their lactation periods were 239.7, 173.2, 214.3, 236.2 and 234.2 days, respectively. 2. The milk performances of the first filial, the second, the third graded generation and Saanen were significantly higher than those of Native Goats. The increment of milk character is the filial, the second, and the third graded generation, compared to the Native Goats were 197.15, 263.94 and 281.79㎏ in milk production; 41.14, 63.05 and 61.05 days in lactation period; -0.34, -1.42 and -1.48% in milk fat percentage. 3. The difference of milk production, lactation period and milk fat percentage between the third and the second graded generation was 17.89㎏, -2.00 days and -0.06, respectively. The difference between purebred Saanen and the third graded generation was 51.15㎏ for milk production, 1.55 days for lactation period, and -0.08% for milk fat percentage. However there was no significant difference between them. 4. The individual comparisons among the least square estimates showed that the difference between the 3 and 1 kid per birth was 130.4㎏ for milk production, 29. 4 days for lactation period, and -0.7% for milk fat percentage. The difference between the 3 .and 2 kid per birth was 3.13㎏, 11.6 days and 0.02%, respectively. The difference between the 2 and 1 kid per birth was 41.6㎏, 17.8 days and 0.09%, respectively.