Galanin이 흰쥐 결장운동에 미치는 영향

진영주 ( Young Joo Chin ),임정묵 ( Joung Muk Leem ),채희복 ( Hee Bok Chae ),박선미 ( Seon Mee Park ),윤세진 ( Sei Jin Youn ) 대한소화기학회 2003 대한소화기학회지 Vol.41 No.3

Background/Aims: Galanin is distributed in enteric nerve terminals throughout the gastrointestinal tract. However, the effect of galanin on colonic motility in isolated colon is still unknown. Using isolated, vascularly perfused rat colon, we investigated the role of galanin on colonic motility, the difference in the effect of galanin between proximal and distal colon, and the innervation of the action of galanin on colonic motility (cholinergic or adrenergic input). Methods: An isolated rat colon was placed in temperature-controlled water bath and vascularly perfused with Krebs solution. After a controlled period, galanin was administered intraarterially and the intraluminal pressure was monitored via microtip catheter pressure transducers at the proximal and distal colon. After pretreatment of phentolamine, propranolol, hexamethonium or atropine, respectively, galanin was infused at a concentration of 240 pM and intraluminal pressure was monitored. The contractile response was expressed as % changes of motility indices over basal period. Results: Galanin significantly increased colonic motility (p<0.01). The contractile response was more potent at the distal colon but the difference was statistically insignificant (p>0.05). The stimulating effect was not inhibited by phentolamine, propranolol, hexamethonium but significantly inhibited by atropine. Conclusions: Galanin increases rat colonic motility and the action of galanin is influenced by cholinergic input. (Korean J Gastroenterol 2003;41:196-203)

급성 아황산가스 폭로후 흰쥐의 폐에 유발된 염증반응에 관한 연구

진영주 ( Young Joo Chin ),박남규 ( Nam Gyu Prak ),이현숙 ( Hyean Suk Lee ),김대수 ( Dae Soo Kim ),엄재호 ( Jae Ho Earm ),조명찬 ( Myeong Chan Cho ),윤세진 ( Sei Jin Yoon ),정화숙 ( Hwa Sook Jeong ),송형근 ( Hyung Geun Song ),성노 대한결핵 및 호흡기학회 1994 Tuberculosis and Respiratory Diseases Vol.41 No.4

폐포단백증에서 동시에 실시한 좌우측 전폐세척

진영주(Young Joo Chin),최기원(Ki Won Choi),이현숙(Hyeon Suk Lee),김대수(Dae Soo Kim),엄재호(Jae Ho Earm),조명찬(Myeong Chan Cho),윤세진(Sei Jin Yoon),박종국(Joong Cook Park),임승운(Seung Woon Lim),이상도(Sang Do Lee) 대한내과학회 1995 대한내과학회지 Vol.49 No.1

Pulmonary alveolar proteniosis is a rare disease of unknown etiology characterized by the accumulation of surfactant like phospholipids and proteinaceous material in the alveolar space. ther are 6 published reports in Korea and only one case of therapeutic whole lung lavage is reported. Whole lung lavage is regarded as the only consistently success ful treatment modality and has been gernerally done, each lung separated by an interval of 2 to 7 days in the adult. In this case, we performed the lavage nf both lungs sequentially in one session and so reduced the number of anesthesia and the duration of hospitalization. After this procedure, we observed a significant improvement in clinical symptoms and pulmonary function test. 2 cases of bilateral whole lavage in one session has been repotrer1 worldwide. There seems to be no significant problem in bilateral whole lung lavage in one session if it is performed cautiously by a specialist. And total lung compliance can be used as a useful indicator for determining the time of the second lavage.

흰쥐의 Trinitrobenzene Sulfonic Acid ( TNBS ) 유발성 대장염에서 Flavonoids 계 Eupatilin 유도체 DA - 6034 의 효과

장동경(Dong Kyung Chang),진영주(Young Joo Chin),정현채(Hyun Chae Jung),송인성(In Sung Song),김정룡(Chung Yong Kim),손미원(Mi Won Son),유무희(Moo Hi Yoo) 대한내과학회 1998 대한내과학회지 Vol.55 No.3

Objective: In traditional medicine, Artemisia capillaris has been used for treatment of chronic diarrhea. Previously we found Artemisia capillaris had an effect on rats with TNBS-induced colitis. Eupatilin, a kind of fla- vonoids, may be a probable effective component. To evaluate the effect of a eupatilin derivative compound DA-6034 on the rat with TNBS-induced colitis, we perfomed this study, Methods: Colitis was induced with 1ml of 50 mg/ml TNBS mixed with 60 % ethanol (vol/vol) in Sprague Dawley rats. From the next day, 1ml methylcellulose, 1 mg/kg prednisolone, 0.3 or 3 mg/kg of DA-6017 and DA-6034 were administered through once daily for 2 weeks. At 2days, lweek, and 2weeks later, we evaluated the effect by gross damage score (0-10) and measured myeloperoxidase, PGE2, and L from the damaged mucosa. Results: The mean gross damage scores of prednisolone end 3 mg/kg of DA groups were significantly lower than that of a placebo group at 2weeks(0.8, 0.9 vs. 4.0, p<0.05). Myeloperoxidase activities also seemed to be lower in those effective groups but were not statistically significant. LTB4 levels were lower in prednisolone and, 0.3 and 3 mg/kg of DA groups than in a placebo group at 2weeks(7.91, 7.23, and 7.13 vs, 13.90 ng/mg protein, p<0.05), PGE2 levels were decreased in prednisolone and 0.3 mg/kg of DA-6034 groups at 2days. DA17 showed no effects. Conclusions: Eupatilin derivative compound, DA-6034 was effective in rats with TNBS-induced colitis. In that LTE4 leve1 is lowered with some decrease of PGE2 level, this agent probably has an inhibitory effect on arachidonic acid metabolism.

Inverse Effort Dependence 가 노력성호기류량의 측정에 미치는 영향

김대수(Dae Soo Kim),이현숙(Hyeon Suk Lee),진영주(Young Joo Chin),엄재호(Jae Ho Earm),조명찬(Myeong Chan Cho),윤세진(Sei Jin Yoon),이상도(Sang Do Lee) 대한내과학회 1995 대한내과학회지 Vol.48 No.6

Objectives: During forced expiratory vital capacity maneuvers, if volume is measured at mouth instead of body surface, the flow during the effort independent portion becomes inversely effort dependent. This negative effect largely depends on compression of thoracic gas and has been termed inverse effort dependence. We performed this study to determine how the test results of FEV1, FEF25-75, FEF25, FEF50, and FEF75 are affected by effort. Methods: We compared the test results selected by effort based criteria(highest PEFR) with that selected by volume based criteria(ATS criteria). And we also studied the correlations between effort dependent changes of each paramaters. Randomly sampled routine pulmonary function test results obtained from 50 normal subjects and 45 subjects with obstructive pulmonary disease were analyzed. Results: 1) In both normal and abnormal group, selection based on the highest PEFR resulted in a decline of mean FEV1 compared to volume based criteria(p< 0.01), and was accompanied by an even more significant, decrease of FVC(p<0.01). 2) In both normal and abnormal group, there was no significant difference in FEF25-75 FEF25, FEF50, and FEF75 between volume based criteria and effort based criteria. 3) In both normal and abnormal group, there was no significant correlation between relative change of PEFR and effort dependent change of FEV1. 4) In normal group there was significant between effort dependent change of FEV1 and accompanying change of FVC(r=0.55, p<0.01). 5) In abnormal group there was significant, correlation between effort dependent change of FEV1 and accompanying change of FVC (r=0.69, p<0.01). Conclusion: It can be concluded that FEV is little affected by effort once the lungs are fully inflated and FEF25-75, FEF25, FEF50, and FEF75 are not affected significantly by inverse effort dependence in clinical practice.

식도 편평상피 유두종 5예

이현숙(Hyeon Suk Lee),김대수(Dae Soo Kim),진영주(Young Joo Chin),윤세진(Sei Jin Youn),송형근(Hyeong Keun Song),성노현(Ro Hyun Sung),이동호(Dong Ho Lee) 대한소화기학회 1995 대한소화기학회지 Vol.27 No.3

Squamous cell papilloma of the esophagus that originates from esophageal mucosa is rare, benign tumor. The etiology is unknown. The endoscopic appearance of the squamous cell papilloma is seen as a benign sessile lesion projecting from the esophageal wall. Patients are free of symptoms directly attributable to the lesion and usually is an incidental finding at endoscopy. We observed endoscopically 5 cases of squamous cell papilloma of the esophagus and endoscopical therapy was performed 3 cases. So, the relevant literatures on the subject were reviewed. (Korean J Gastroenterol 1995;27:356 - 360)

쯔쯔가무시병에 동반된 간질성 폐렴에 관한 연구

이현숙(Hyeon Suk Lee),김대수(Dae Soo Kim),진영주(Young Joo Chin),김성진(Seong Jin Kim),송형근(Hyeong Keon Song),성노현(No Hyeon Seong),이상도(Sang Do Lee),김명수(Myeong Su Kim) 대한내과학회 1996 대한내과학회지 Vol.50 No.1

Objectives: Interstitial penumonia is a major cause of death in scrub typhus but little is known about its pathology and pathogenesis. To study the clinical findings, pathology and pathogenesis of interstitial pneumonia in scrub typhus, we performed this study. Methods: We performed clinical evaluation, laboratory tests, radiological evaluation including high resolution CT and pulmonary function tests in 53 scrub typhus patients. Bronchoalveolar lavage(BAL) and transbronchial lung biopsy(TBLB) were done in patients with interstitial pneumonia and electron microscopic examination and immunofluorescence study including monoclonal antibody against Rickettsia tsutsugamushi were done. Results: 1) Interstitial pneumonia was found in 13(26%) patients. 2) Radiological findings: High-resolution CT showed diffuse ground glass opacity, thickening of bronchovascular bundie and prominent central core of secondary pulmonary lobule with minimal thickening of interlobular septum and there were no findings suggesting vasculitis 3) Cells in HAL fluid: total cell count, (46±9)×104/ml, alveolar macrophage, 68±17%, lymphocyte, 28±15% and neutrophil, 5±3% Pathologic finding(TBLH): 4) Light microscope: interstitial edema with mononuclear cell infiltration, mainly lymphocyte, without any evidence of vasculitis or perivasculitis 5) Immunofluorescence and eletron microscope ' IF study, using anti-Ig G,A,M, anti-Cg, anti-fibrinogen and anti-rickettsial Ab, revealed negative reaction. Electron photomicrograph revealed no rickettsial organism. Conclusion: In interstital pneumonia associated with scrub typhus we noted lymphocytosis in BAL fluid and interstitial inflammation in histopathology without any evidence of vasculitis. Thus vasculitis is not the only main pathology but other mechanism including immunopathogenesis may operate in the pathogenesis of intersititial pneumonia in scrub typhus

폐쇄성 담관질환의 담즙에서의 Helicobacter 검출에 관한 연구

노임환(Im Hwan Roe),이문숙(Moon Suk Lee),진영주(Young Joo Chin),임창영(Chang Young Lim),송일한(Il Han Song),김정원(Jung Won Kim),신지현(Ji Hyun Shin),이학성(Hak Sung Lee),이종화(Jong Hwa Lee) 대한내과학회 1998 대한내과학회지 Vol.55 No.3

Objective: Several newly recognized Helicobacter spp. such as H. hepaticus, H. bilis, H, cholecystus, H. rappini, H. pullorum, can cause persistent hepatitis, hepatoma, cholangiopancreatitis, and cholecystitis in animals. Recently same studies have been reported that Helicobacter DNA can be found in the bile from the patients with diseased bile duct, although its clinical significance is still unclear. We aim of this study is to investigate the existence, and character of Helicobacter in the bile from the obstructed bile duct, and the relationship with pH and the other bacteria found in the bile. Methods: Twenty-eight bile samples (15 from bile duct cancer, 6 from pancreatic head cancer, 7 from bile duct stones) were obtained from the PTBD route. Bile pH measurement, and Helicobacter culture in microaerophilburic and anaerobic conditions were performed. The primers chosen for polymerase chain reaction(PCR) amplification for detection and characterization were ureA (411 bp) and cagA gene (298 bp), respectively. And primer of 16s rRNA for all known bacteria including Helicobacter was used, and the kinds of bacteria were identified by RFLP. Results: Helicobacter DNA was detected in 39.3%. The bile pH was not related with presence of Helicdxxter (7.83±0.41 vs 7.78±0.48). The prevalence of cagA was 35.7%, and 16s rRNA was found in 46.4%. The specific 16s rRNA band for Helicobacter was observed in 14.3%. All the culture were not successful. Conclusion: Although the Helicobacter spp. were not cultured, Helicobacter exists obviously in the bile from the diseased bile duct, and coexist with other bacteria. These results should stimulate studies to ascertain whether these Helicobacter play a role in the pathogenesis of bile duct diseases in human.

한국에서 분리된 Helicobacter pylori 균주에서의 병리 인자 분포 및 이에 대한 숙주 방어 기전의 분자생물학적 연구

김정목(Jung Mogg Kim),정현채(Hyun Chae Jung),이주영(Joo Young Lee),이경미(Kyung Mee Lee),진영주(Young Joo Chin),김영전(Young Jeon Kim),송인성(In Sung Song),김정룡(Chung Yong Kim) 대한내과학회 1998 대한내과학회지 Vol.54 No.1

Objectives: CagA or cytotoxin-positive H. pylori may be associated with gastroduodenal diseases. However, controversies about this association also exist. Moreover, there could be geographic differences in the prevalence of virulence factors such as cagA or cytotoxin. In H. pylori infection, the gastric mucosa shows acute and chronic inflammation. However, the pathogenesis of such as an inflammation by H. pylori is not well elucidated. We performed this study 1) to determine prevalence of the genes of virulence factor such as cagA and cytotoxin in H. pylori, 2) to assess the correlation of their presence with clinical findings, and 3) to test whether the vacuolating cytotoxin of H. pylori could evoke proinflammatory cytokine gene expression in gastric epithelial cells. Methods: 1) The prevalence of the cagA, vacA and adhesin genes in H. pylori strains isolated from Koreans was determined by PCR analysis. 2) H. pylori was cultured in Brucella broth containing 10% fetal bovine serum for 3 days using a shaker in a microaerophilic condition. Cytotoxin assay was performed by determining whether addition of the concentrated culture supernatants is able to cause vacuolization of HeLa cells. 3) After human gastric epithelial cells, Hs746T and AGS were incubated with the culture supernatants containing vacuolating cytotoxin, each RNAs were extracted from the gastric epithelial cells. And then various cytokine gene expression were assessed using RT-PCR. The expressed cytokine transcripts were quantified by RT-PCR and standard synthetic RNA. Among cytokines, IL-8 proteins were also measured by ELISA. Results: 1) More than 95% of H. pylori isolates from Korean adults possessed cagA, vacA and adhesin genes. And 80.6% of H. pylori strains have expressed vacuolating cytotoxicity against HeLa cells within 24 hours. 2) There was no correlation between the virulence factors of H. pylori strains and clinical findings. 3) Cytotoxin-positive culture supernatants also caused vacuolization in gastric epithelial cells, both Hs746T and AGS. 4) Expression of mRNA for proinflammatory cytokines such as IL-1 α, IL-8, MCP-1 and GM-CSF was much more upregulated by vacuolating cytotoxin-positive culture supernatants than cytotoxin-negative ones in both Hs746T and AGS cells. Number of molecules of the expressed IL-8 transcripts was parallel to the amounts of IL-8 protein secreted from gastric epithelial cells. Conclusion : These results suggest that virulence factors of H. pylori may not be factors determining disease entitiy in Korean patients infected with H. pylori. In addition, vacuolating cytotoxin secreted from H. pylori could give rise to vacuolization in gastric epithelial cells as well as induce proinflammatory cytokines from the cells.

간경변증 환자에서 Ranitidine의 약동학적 연구

진영주,엄재호,윤세진,이동호,장인진,신상구 大韓消化器病學會 1998 大韓消化器病學會誌 Vol.32 No.5