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지관자(KJ Jee),김석찬(SC Kim),지일운(IW Ji),문영규(YG Moon) 대한산부인과학회 1996 Obstetrics & Gynecology Science Vol.39 No.12
In the future, fetal cells in the maternal blood may be a source of material for routine screening for prnatal diagnosis in the first trimester for any woman who wishes to be tested. In order for this to become a reality, the timing of the appearance of fetal cells in the maternal circulation must be defined accurately. So, our work have focused on when during gestation can fetal cells best be recovered and whether they are then present and available in sufficient numbers for analysis throughout the pregnancy. Samples were collected from 162 women pregnancy between 7 and 25 weeks of getation and we analyzed the nRBC ratio(%) within total isolated nucleated cells by differential counts(Diff-Quik stained). In this study, we have some conclusions; The results of our study of fetal cell isolation was very encouraging(sucessful rate: 91.35%). It is possible to isolation of nRBC in all pregnant women during 9~25 weeks of pregnancy, although the best period for fetal cell recover was 11~15 weeks of gestation.
지관자(KJ Jee),김석찬(SC Kim),김구련(GR Kim),지일운(IW Ji),정재현(JH Jeong),문영규(YG Moon) 대한산부인과학회 1997 Obstetrics & Gynecology Science Vol.40 No.5
The isolation of fetal cells from maternal circulation has the potential to allow relatively self prenatal diagosis for all pregnant women. The present technology, however, has not reached the accuracy required for clinical diagnosis because of maternal cell contamination So we published a new method for enrichment of nRBC in a fetal cell isolation(1996). In this study, attempted to FISH analysis of nRBC which was isolated by our own methods. We evaluated the efficiency of FISH. As the results, we have successfully used FISH on enriched nRBC. We were able to identified 2 abnormal fetus which were confirmed by conventional cytogenentic study as Down syndrome(Fig.1) and Klinefeltre syndrome(Fig.2). And the sensitivity and specificity for FISH was 86%(49/57) and 92.3%(36/39), respectively. According to our results, fetal cell analysis by FISH can be reliable used for prenatal aneuploidy diagnosis. However, the problems of enrichment of the fetal cell and FISH probe or condition should be over come before analyze.
GPA ( glycophorin A ) 와 FISH를 결합시킨 방법에 의한 모체혈액 내 태아세포를 이용한 유전학적 연구
지관자(KJ Jee),김석찬(SC Kim),이승수(SS Lee),문영규(YG Moon) 대한산부인과학회 1998 Obstetrics & Gynecology Science Vol.41 No.5
The possibility of prenatal genetic screening using fetal cells isolated from maternal blood has been reported. However, it is essential to recognize of real fetal nRBC for an accurate prenatal diagnosis because the number of fetal cells in maternal circulation is very rare and some maternal cells can be contaminated in it, in spite of enrichment of maternal pheriperal blood. Therefore, we attempted the method called Immuno-FISH that GPA (glycophorin A) conjugated to cytoplasmic membrane and a DNA probe tagging fluorescene with binding to nucleus, simultaneously, for more efficacious analysis of fetal genetic screening. As the results of evaluated from 42 pregnancy women; we could identify 8.2 fetal nRBCs from 20 ml maternal pheriperal blood, we can observe the signal of Immuno-FISH onto nRBC within 16 on the 42 samples (35.6%), successfully, and we could have more efficacious results as the sensitivity was 71.74%, the specificity was 88.8%.
쥐 유방암종에서 흉선세포 융합에 의한 전이능 증가 기작에 관한 연구
김철우,지관자,김종재 한국유전학회 1993 Genes & Genomics Vol.15 No.2
We have obtained three hybrid cell lines- FA4, FB4 and FB6 - through hybridization process between rat NMU mammary carcinoma cell line and allogeneic thymocytes. Those hybrid cell lines were inoculated with matrigel into the interscapular subcutaneous tissue in newborn Sprague-Dawley rats. About 5-14 weeks later, metastatic nodules were developed in the lungs and axillary lymph nodes among the animals, which were inoculated by FB6 or transplanted subsequently by those tumor masses. The expression of Thy 1.1 and T cell antigen was higher in FB6-12, one of the established cell lines which were gained from metastatic tumor lesions by inoculation of FB6, than in other cell lines. Also we have obtained FB6-HM subclone which was selected as a highly metastatic clone from FB6 by limiting dilution method. The evidence of hybridization between NMU and thymocytes was disclosed by the fact that FB6-HM clone showed a positive reaction against polyclonal rabbit sera raised by repeated immunization with S-D rat thymocytes. CD44 molecule, which has been known that the degree of its expression in the neoplastic epithelial cells was positively correlated with the increased metastatic potential, was also more expressed in FB6-12 than in any other cell lines. The results of this study strongly supported the hypothesis that epithelial tumor cells could gel metastatic potential through hybridization with lymphoreticular lineage cells. Additionally, by establishing the metastasizing mammary carcinoma in Sprague-Dawley rat model, we could perform proper experiments on the study of mechanism of hematogenous and lymphatogenous metastasis in cancer.