Bacillus spp. 엽면살포에 의한 가로수 및 고추의 병 방제

정준휘,류충민 한국식물병리학회 2016 식물병연구 Vol.22 No.2

Out of plant-associated bacteria, certain plant growth-promoting bacteria (PGPB) have been reported to increase plant growth and productivity and to elicit induced resistance against plant pathogens. In this study, our objective was to broaden the range of applications of leaf-colonizing PGPB for foliar parts of road tress and pepper. Total 1,056 isolates of endospore-forming bacteria from tree phylloplanes were collected and evaluated for the enzymatic activities including protease, lipase, and chitinase and antifungal capacities against two fungal pathogens, Colletotrichum graminicola and Botrytis cinerea. Fourteen isolates classified as members of the bacilli group displayed the capacity to colonize pepper leaves after spraying inoculation. Three strains, 5B6, 8D4, and 8G12, and the mixtures were employed to evaluate growth promotion, yield increase and defence responses under ἀeld condition. Additionally, foliar application of bacterial preparation was applied to the road tress in Yuseong, Daejeon, South Korea, resulted in increase of chlorophyll contents and leaf thickness, compared with non-treated control. The foliar application of microbial preparation reduced brown shot-hole disease of Prunus serrulata L. and advanced leaf abscission in Ginkgo biloba L. Collectively, our results suggest that leaf-colonizing bacteria provide potential microbial agents to increase the performance of woody plants such as tree and pepper through spray application.

브레이크 냉간저더와 디스크 두께편차 및 런아웃의 관계에 관한 실험 분석

정준휘(Jun hui Jeong),신응수(Eung soo Shin) 한국자동차공학회 2021 한국자동차공학회 학술대회 및 전시회 Vol.2021 No.11

브레이크 디스크 두께 편차와 런아웃이 냉간 저더에 미치는 영향에 관한 실험 분석

정준휘(Jun hui Jeong),신응수(Eung soo Shin) 한국자동차공학회 2022 한국 자동차공학회논문집 Vol.30 No.5

Twist Friction Driver의 이송특성에 관한 연구

정준휘(Jun Hui Jeong),이응석(Eung Suk Lee),안동율(Dong Yul An) 대한기계학회 2004 대한기계학회 춘추학술대회 Vol.2004 No.11

This paper propose a study on the Feed Characteristics of Twist Friction Driver. We are using Twist Friction Driving mechanism system. The system consists of Twist Friction Driver elements such as driving shaft, driven roller, Spring for pre-load, Air bearing guide, Servo motor, and measuring devices such as Encoder of Servo motor, Laser interferometer, LVDT . The Twist Friction driver is mechanically simple and very quiet at high speed, and has low pre-load. So The Twist Friction driver can materialize an ultra precision feed-resolution. The feed characteristics of the driver is determined by slip and angular error, backlash.

새로운 생물적 방제 전략: 미생물 인자 유래 식물면역 유도

이상무,정준휘,류충민 한국식물병리학회 2015 식물병연구 Vol.21 No.2

새로운 생물적 방제 전략: 미생물 인자 유래 식물면역 유도

이상무,정준휘,류충민 한국식물병리학회 2015 식물병연구 Vol.21 No.3

Plant have developed sophisticated defence mechanisms against microbial pathogens. The recent accumulated information allow us to understand the nature of plant immune responses followed by recognition of microbial factors/determinants through cutting-edge genomics and multi-omics techniques. However, the practical approaches to sustain plant health using enhancement of plant immunity is yet to be fully appreciated. Here, we overviewed the general concept and representative examples on the plant immunity. The fungal, bacterial, and viral determinants that was previously reported as the triggers of plant immune responses are introduced and described as the potential protocol of biological control. Specifically, the role of chitin, glucan, lipopolysaccharides/extracellular polysaccharides, microbe/pathogen-associated molecular pattern, antibiotics, mimic-phytohormones, N-acyl homoserine lactone, harpin, vitamins, and volatile organic compounds are considered. We hope that this review stimulates scientific community and farmers to broaden their knowledge on the microbial determinant-based biological control and to apply the technology on the integrated pest management program. 식물은 다양한 병원성 미생물에 대하여 효과적인 방어 기제를 발전시켜 왔다. 최근 유전체와 다중 오믹스 기술의 발전은우리에게 미생물 인자에 의한 식물 면역을 폭넓게 이해할 수 있는 단초를 제공해 주었다. 하지만 아직까지는 이러한 기술을 병방제 전략에 이용한 적은 많지 않다. 그래서 본 리뷰에서 식물면역의 기본 개념을 소개하고 최근 얻어진 결과들을 소개하였다. 덧붙여 이미 논문에서 발표된 진균, 세균, 바이러스 유래 결정인자에 의한 생물적 방제 가능한 방법에 대해 기술하였다. 특히 미생물 결정인자인 chitin, glucan, LPS/EPS, 미생물분자패턴, 항생제, 식물유사호르몬, AHLs, harpin, 비타민, 휘발성물질에대한 결과를 자세하게 기술하였다. 이 리뷰를 통하여 많은 과학자들과 농민들이 미생물 결정인자 기반의 생물적 방제에 대한지식이 폭넓어지고, 다양한 미생물 결정 인자가 앞으로 농업현장의 종합적인 병방제 전략의 하나로 자리매김하기를 바란다.

무 유전자원 82 계통의 유리당 함량 분석

서미숙,정준휘,박범석,김정선 한국자원식물학회 2018 한국자원식물학회지 Vol.31 No.5

십자화과 식물에 속하는 무는 전세계적으로 재배되는 중요한 뿌리 채소작물이다. 본 연구에서는 다양한 형질을 가진 무 유전자원 82 계통을 대상으로 총 유리당 함량을 분석하였다. 일본, 독일, 그리고 한국에서 보유중인 82 계통의 유전자원은 크게 5 아종을 포함하고 있으며, 야생형, 야생 근연종, 재래종 그리고 육성품종으로 구분된다. 포도당, 과당, 자당 그리고 엿당을 분석하여 총 유리당 함량을 측정한 결과, 평균 25.33 ㎎/g, 5.64-46.68 ㎎/g의 분포도를 나타내어 계통에 따라 총 유리당함량에 차이를 확인할 수 있었다. 또한 총 유리당 함량에 대하여포도당이 56.92%로 가장 높은 유리당 함유율을 보였고, 과당(31.34%), 자당(10.46%), 그리고 엿당(2.25%)의 순으로 높은 함유 율을 보였다. 5 아종에서 유전형에 따른 총 유리당 함량의 차이는 관찰되지 않았으나, 아종에 따른 유리당 함유 율의 차이는확인할 수 있었다. 또한 야생종과 야생 근연종 그리고 재래종 무는 육성 품종과 비교하여 평균 총 유리당 함량에서는 큰 차이를보이지 않았으나, 당의 종류별 함유 율을 비교한 결과 야생종과재래종에서 높은 함유 율을 확인할 수 있었다. 본 실험의 결과는후속 연구를 통한 고품질 무의 개발 및 무의 유리당 생합성 관련연구를 위한 기초정보를 제공할 수 있을 것이다. Radish (Raphanus sativus L.) is a species of the Brassicaceae family and an important root vegetable crop, produced worldwide. A total of 82 radish accessions with various morphological and physiological characteristics analyzed for total sugars content. These accessions includes five subspecies and classified as wild, wild-relative, traditional and improved cultivar. The four sugars, glucose, fructose, sucrose and maltose, showed various contents in 82 accessions. Total sugar content ranged from 5.64 to 46.68 ㎎/g and showed average 25.33 ㎎/g. Total sugar content was not statistically significant among the five subspecies, but individual sugar ratio varied. The wild, wild-relatives and traditional cultivars were not significantly among average total sugars content compared with improved cultivars. On the other hand, the wild and traditional cultivars were showed high ratio of individual sugars. These results could be valuable information for the development of new radish cultivars and regulation of sugars biosynthesis in radish.

Complete genome sequence of polybutylene adipate terephthalatedegrading Rhizobacter sp. strain SBD 7-3, isolated from soil

연제형,최한석,정준휘,고영준,김다연,안시현,안재형,원항연 한국미생물학회 2023 미생물학회지 Vol.59 No.4

Rhizobacter sp. SBD 7-3, a polybutylene adipate terephthalate (PBAT)-degrading strain, was isolated from upland soil that has been installed by biodegradable mulching films in Yeongwol province, Republic of Korea. The genome analysis revealed strain SBD 7-3 genome comprised one chromosome (5,594,180 bp) and two plasmids (200,976 bp; 142,941 bp), with G + C contents of 67.6%, 60.7%, and 61.4% respectively. The genome contained 5,540 protein-encoding genes, 58 tRNA genes, and 3 rRNA genes. Several genes linked to the breakdown of PBAT are found throughout the genome.

중대재해처벌법 인지 정도와 건설 근로자 안전 의식 수준의 상관관계분석

서영준,김슬기,이동엽,정준휘,김대영,Seo, Youngjun,Kim, Seulgi,Lee, Dongyeope,Jung, Junhwi,Kim, Daeyoung 한국건설관리학회 2024 한국건설관리학회 논문집 Vol.25 No.3

본 연구의 목적은 중대재해처벌법에 따른 건설 노동자의 중대재해인지 수준과 안전의식 수준 간의 상관관계를 분석하는 것이다. 건설 노동자, 건설 관리자, 안전 관리자를 대상으로 설문조사를 실시하였다. 이 연구의 결과는 다음과 같다. 세 그룹 간의 상관관계 분석 결과, 안전 관리자가 가장 높은 상관관계를 보였으며, 그 다음으로 건설 관리자와 건설 노동자가 이어지며, 세 그룹 모두 보통 수준의 상관관계를 나타냈다. 전체 그룹에서의 상관관계 분석도 유의한 상관관계를 나타내었으며, 참가자 수가 증가함에 따라 더 높은 상관계수가 관찰되었다. 또한, 상관계수의 유의성을 검증하기 위해 p-value와 유의수준(α)을 비교하였다. 결과적으로 유의수준인 0.05보다 작은 p-value를 얻어 귀무가설을 기각하고 대립가설인 "중대재해처벌법에 대한 인지 정도와 안전의식 수준 정도는 관계가 있다"를 채택하다. 따라서, 중대재해처벌법의 인지 정도와 건설 안전의식 수준은 관련이 있다는 결론을 얻었다. 이 연구의 한계점은 주관적인 지표를 기반으로 한 설문조사에 의존하였으므로, 질문 항목의 난이도에 변동성이 존재할 수 있다는 점이다. The purpose of this study was to analyze the Correlation Analysis between Awareness of the Serious Disaster and Safety Consciousness of Construction Workers under the Act on the Punishment of Serious. A survey was conducted on construction workers, construction managers, and safety managers. The results of this study were as follows; The correlation analysis conducted among the three groups indicated a significant correlation, with safety managers demonstrating the highest correlation, followed by construction managers and construction workers, and all three groups exhibited a moderate correlation. The correlation analysis conducted for the entire group also revealed a significant correlation, and as the number of participants increased, higher correlation coefficients were observed. Furthermore, to ascertain the significance of the correlation coefficients, a comparison was made between the p-value and the significance level (α). Consequently, a p-value smaller than the significance level of 0.05 was obtained, leading to the rejection of the null hypothesis and the acceptance of the alternative hypothesis. Therefore, it can be concluded that there is a relationship between the level of awareness of the serious Accidents Punishment Act and the level of Safety Consciousness of Construction. One limitation of this study is that it relied on a subjective indicator through a survey, which may introduce variability in the difficulty level of the questionnaire items.

미생물제(Sphingobium sp. Cam5-1) 처리에 따른 토양 중 카두사포스의 분해효과

연제형 ( Jehyeong Yeon ),정준휘 ( Joon-hui Chung ),최한석 ( Han Suk Choi ),고영준 ( Young-joon Ko ),김다연 ( Dayeon Kim ),안시현 ( Sihyun An ),안재형 ( Jae-hyung Ahn ),한귀환 ( Gui Hwan Han ),원항연 ( Hang-yeon Weon ) 한국환경농학회 2023 한국환경농학회지 Vol.42 No.4

Cadusafos, an organophosphorus insecticide, has been commonly used against various pests worldwide. Organophosphorus pesticides have shorter half-lives and lower toxicities than organochlorine pesticides. However, excessive use of Cadusafos can increase pest resistance and issues with acetylcholine biomagnification, potentially resulting in human toxicity. In this study, we investigated the effect of a Cadusafos-degrading microbial agent (CDMA) prepared using Sphingobium sp. Cam5-1, which was previously reported to effectively degrade residual Cadusafos in soil. Experiments were conducted under both controlled laboratory and greenhouse field conditions. Under laboratory conditions, CDMA (10⁶ cfu/g soil application rate) decomposed 97% of Cadusafos in the soil in the untreated control after 21 days. Additionally, when CDMA (10⁶ cfu/g soil) was mixed with quicklime, 99% of Cadusafos was decomposed within 3 days. Under greenhouse field conditions, the combined effect of CDMA (10⁶ cfu/g soil) and quicklime was not observed. However, CDMA (10⁶ cfu/g soil) application alone was capable of decompos- ing 91% of Cadusafos after 3 days. These results indicate that CDMA can effectively decompose high residual levels of Cadusafos in soils under field conditions using a low inoculum rate.