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쥐 해마에서 글루타메이트의 시간에 따른 변화가 급성 스트레스 연구에 미치는 영향 : 자기공명분광학을 이용한 연구
연제형(Jehyeong Yeon),윤창수(Chang-Soo Yun),황윤호(Yoonho Hwang),백현만(Hyeon-Man Baek),김동윤(DongYoun Kim),한봉수(Bong Soo Han) 대한전자공학회 2021 대한전자공학회 학술대회 Vol.2021 No.6
It was reported that rodents were significantly influenced by stress from experimental procedure and by isoflurane anesthesia. Therefore, in MRS experiments in the hippocampus of normal mice, the concentrations of Glu and tCho were observed to decrease over time. Glu is a potential stress biomarker and its concentration increases with stress. The purpose of this study is to investigate the effect of the time delay that may occur during 1H-MRS experiments on acute stress studies by comparing the control group and the stress group. 20 C57BL/6N male mice were grouped into the control group and the stress group. The stress group mice were exposed to acute constrain stress, and 1H-MRS data were obtained from the left hippocampus, and the difference in the concentration of metabolites due to stress was compared. We found that the statistical significance of the difference in Glu concentration between the two groups changed if there was a difference of about 17 minutes in the data collection time between the two groups. In conclusion, the results of this study suggest that it is necessary to avoid the difference in the timing of data collection between the control and experimental groups in stress studies.
미생물제(Rhodococcus sp. 3-2) 처리에 따른 토양 중 카벤다짐의 분해효과
연제형 ( Jehyeong Yeon ),김현수 ( Hyeon-su Kim ),안재형 ( Jae-hyung Ahn ),한귀환 ( Gui Hwan Han ),오영곤 ( Young Goun Oh ),조일규 ( Il Kyu Cho ),박인철 ( In-cheol Park ) 한국환경농학회 2021 한국환경농학회지 Vol.40 No.4
BACKGROUND: The fungicide of benomyl, a benzimidazole group, has been commonly used for pesticides against fungal diseases in the world. However, benomyl is rapidly hydrolyzed in the environment after using to control plant diseases and has adverse effects by generating carbendazim, which is toxic to plants, humans, and the environment. METHODS AND RESULTS: In this study, the decomposition effect of carbendazim, a degradation product of benomyl was conducted in pot and field after making a prototype of benomyl-degrading microbial agent (BDMA). We found that the carbendazim-degrading microbial agent (CDMA) (10<sup>5</sup>, 10<sup>6</sup>, and 10<sup>7</sup> cfu/g soil) decomposed carbendazim by 50% or more in all the treatments, compared to the untreated control in the pot tests after four weeks. The effect of 100% decomposition of carbendazim was observed at 7 days after treatment, when the prototype of BDMA was apllied at 10-folds dilution in the field. The decomposition effect at more than 60% and plant growth promoting effect were observed after 7 days of the treatment, compared with the untreated group in the second field experiment,treated with commercially available concentrations of 500-folds and 1,000-folds. CONCLUSION(S): These results might represent that the BDMA would decompose carbendazim effectively, a decomposition product of the fungicide benomyl, remaining in agricultural area, and it could be utilized practically by using a low dilution rate.
미생물제(Sphingobium sp. Cam5-1) 처리에 따른 토양 중 카두사포스의 분해효과
연제형 ( Jehyeong Yeon ),정준휘 ( Joon-hui Chung ),최한석 ( Han Suk Choi ),고영준 ( Young-joon Ko ),김다연 ( Dayeon Kim ),안시현 ( Sihyun An ),안재형 ( Jae-hyung Ahn ),한귀환 ( Gui Hwan Han ),원항연 ( Hang-yeon Weon ) 한국환경농학회 2023 한국환경농학회지 Vol.42 No.4
Cadusafos, an organophosphorus insecticide, has been commonly used against various pests worldwide. Organophosphorus pesticides have shorter half-lives and lower toxicities than organochlorine pesticides. However, excessive use of Cadusafos can increase pest resistance and issues with acetylcholine biomagnification, potentially resulting in human toxicity. In this study, we investigated the effect of a Cadusafos-degrading microbial agent (CDMA) prepared using Sphingobium sp. Cam5-1, which was previously reported to effectively degrade residual Cadusafos in soil. Experiments were conducted under both controlled laboratory and greenhouse field conditions. Under laboratory conditions, CDMA (10<sup>6</sup> cfu/g soil application rate) decomposed 97% of Cadusafos in the soil in the untreated control after 21 days. Additionally, when CDMA (10<sup>6</sup> cfu/g soil) was mixed with quicklime, 99% of Cadusafos was decomposed within 3 days. Under greenhouse field conditions, the combined effect of CDMA (10<sup>6</sup> cfu/g soil) and quicklime was not observed. However, CDMA (10<sup>6</sup> cfu/g soil) application alone was capable of decompos- ing 91% of Cadusafos after 3 days. These results indicate that CDMA can effectively decompose high residual levels of Cadusafos in soils under field conditions using a low inoculum rate.