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고추역병균 ( 병원균 : Phytophthora capsici ) 의 생물학적 방제 - Ⅱ. 항균물질의 분리 정제 및 항균활성 -
장윤희,장상문,최정,이동훈 ( Yoon Hee Chang,Sang Moon Chang,Jyung Choi,Dong Hoon Lee ) 한국환경농학회 1996 한국환경농학회지 Vol.15 No.4
In the culture medium, the three antifungal fractions against P. capsici were separated by Sephadex G-25 column chromatography and Silica-gel chromatography. The substance A in white powder and the substance B in sticky oil were isolated by ethyl acetate: acetone mixture(7 : 3), and the substance C in yellow powder was isolated by chloroform: ethyl acetate mixture(95 : 5). The crude extract by ethyl acetate from the culture medium acidified to pH 2 was known to inhibit completely the growth of P. capsici at the level of 50㎎ ㎏^(-1). The substance A and B were known to be effective above the level of 5㎎ ㎏^(-1), and the substance C was effective above the level of 1㎎ ㎏^(-1). However, at the level of 20㎎ ㎏^(-1), the efficiency was in the order of A>C>B. It is apparent on a pot-experiment scale that the three substances effectively control Phytophthora blight of the red-pepper plant grown in the soil inoculated with P. capsici.
고추역병균 ( 병원균 : Phytophthora capsici ) 의 생물학적 방제 - Ⅰ. 고추 역병 길항균의 (拮抗菌) 선발 -
장윤희,장상문,이동훈,최정 ( Yoon Hee Chang,Sang Moon Chang,Dong Hoon Lee,Jyung Choi ) 한국환경농학회 1996 한국환경농학회지 Vol.15 No.3
This study was attempted to select an antagonist against Phytophthora blight of red-pepper caused by Phytophthora capsici. The three strains, A-35, A-67 and A-183 were isolated from the rhizosphere in soil where red-pepper had been cultivated continuously for a long time, and the strain A-83 was estimated to be the strongest antagonist against P. capsici. The A-183 strain was identified as a strain of Pseudomonas sp., showing the maximum antifungal activity, when cultured at 30℃ for 5 days in the potato extract medium(pH 6.5) containing 2.0% mannitol and 0.3% peptone.
무릎 신전 제한형 보조기 착용이 보행 시 하지 내 협응에 미치는 영향
장윤희(Yoonhee Chang),정보라(Bora Jeong),강성재(Sungjae Kang),류제청(Jeicheong Ryu),김규석(Gyu Seok Kim),문무성(Museong Mun),고창용(Chang-Yong Ko) 한국재활복지공학회 2016 재활복지공학회논문지 Vol.10 No.3
본 연구의 목적은 무릎 신전 제한형 보조기 착용 후 보행시 하지 내 협응에 미치는 영향을 평가하는 하는 것이다. 본 연구에는 7명의 건강한 성인 남성(32.3±2.7 세, 175.2±3.8cm, 76.2±8.7kg)이 참여하였다. 이들은 무릎보조기를 착용하거나 착용하지 않고 10m의 평지를 보행하였다. 이때 시공간적 파라미터, 하지의 각도, 하지 내 연속상대위상(CRP)를 측정 및 계산하였다. 그 결과 시공간적 요인은 차이가 없었다 (all p > 0.05). 무릎보조기 착용 시 엉덩관절의 가동범위 및 각도 변화는 없었으나, 무릎관절의 가동범위는 감소하였으며 (65.5±3.7° vs. 60.5±3.5°, p < 0.05), 최대 굴곡 역시 감소하였다 (입각기: 31.9±4.6 vs. 25.6±5.5, 유각기: 76.7±3.1 vs. 68.9±3.4, all p < 0.05). 발목관절의 가동범위는 변화가 없었으나, 최대 배측굴곡 (22.4±2.6 vs. 19.2±2.1, p< 0.05)은 감소하였으며, 최대 족저굴곡이 증가하였다 (9.5±3.0 vs. 15.7±2.2, p<0.05). 대부분의 CRP는 차이가 없었으며, 유각기 엉덩관절과 무릎관절 사이의 CRP만 감소하였다 (93.0±7.8 vs, 84.7±4.9, p < 0.05). CRP의 표준편차는 대부분 증가하였다 (유각기 엉덩관절과 무릎관절사이: 25.1±6.7 vs. 32.4±1.9, 입각기 무릎관절과 발목관절사이: 46.0±12.9 vs. 80.1±31.1, 유각기 무릎관절과 발목관절 사이: 34.5±4.1 vs. 37.6± 3.1, all p < 0.05). 이와 같은 결과로부터 무릎 신전 제한형 보조기 착용은 관절의 각도, 하지 내 협응에는 영향을 미치지만, 보행특성에는 큰 영향을 미치는 않는다는 것을 확인하였다. The aim of this study was to evaluate Influence on intra-limb coordination in individuals wearing knee brace during walking. Seven healthy male adults (32.3±2.7 years old, 175.2±3.8cm, 76.2±8.7kg) participated. They wore knee brace or didn`t wear any knee brace and were asked to walk along a 10 m long walkway. Spatiotemporal parameters, angles of the lower limbs, and intra-limb continuous relative phase (CRP) were measured and calculated. No differences of spatiotemporal parameters were shown (all p > 0.05). There were no changes in the angle and its range of motion (ROM) in the hip for the subjects as wearing knee brace, while ROM (65.5±3.7° vs. 60.5±3.5°, p < 0.05) of the angle and maximum flexion angles (stance: 31.9±4.6 vs. 25.6±5.5, swing: 76.7±3.1 vs. 68.9±3.4, all p < 0.05) in the knee significantly decreased. No changes in ROM of angle in the ankle were shown, whereas maximum dorsiflexion decreased (22.4±2.6 vs. 19.2±2.1, p< 0.05) and maximum plantarflexion increased (9.5±3.0 vs. 15.7±2.2, p<0.05). There were no changes in most of CRP between joints. CRP between the hip and knee joints decreased (93.0±7.8 vs, 84.7±4.9, p < 0.05). Most of CRP standard deviation increased (between the hip and ankle joint during swing: 25.1±6.7 vs. 32.4±1.9, between the knee and ankle joint during stance: 46.0±12.9 vs. 80.1±31.1, between the knee and ankle joint during swing: 34.5±4.1 vs. 37.6±3.1, all p < 0.05). These results indicated that wearing knee brace affected joint angle and intra-limb coordination, but less affected gait features.
장윤희(Yoon Hee Chang),고창용(Chang Yong Ko),정보라(Bo Ra Jeong),강정선(Jung Sun Kang),김규석(Gyoo Suk Kim),류제청(Jei Cheong Ryu) Korean Society for Precision Engineering 2018 한국정밀공학회지 Vol.35 No.1
Gait analysis is the best objective measurement tool for monitoring rehabilitation. However, it has limitations to evaluate gait recovery. Previous studies have evaluated the effect of gait training using continuous relative phase. The objective of this study was to determine the effect of gait recovery by rehabilitation gait training on lower limb coordination. We analyzed spatio-temporal parameters and CRP values of hip and knee joints based on gait analysis data obtained by 3D motion analysis system at 15 days intervals in 24 uni-lateral transfemoral amputees participated in IRP. Our results revealed that walking velocity of uni-lateral transfemoral amputees who participated in the program during a mean of 107.1 days was 49.2% faster than that at initial stage. The walking velocity showed a 46% increase at the end of 30 days after training. In gait coordination, values of CRP-RMS and CRP-SD were increased and maintained in-phase pattern. CRP showed symmetry in both limbs at the end of 90 days after training. Therefore, CRP is a significant factor in the gait recovery process. Effects of various rehabilitation training methods can be determined through CRP analysis.
장윤희(Yunhee Chang),유지형(Jihyung Yoo) 한국자동차공학회 2020 한국자동차공학회 학술대회 및 전시회 Vol.2020 No.11
An exploratory study on non-intrusive thermometry of permanent magnets based on optical fiber pyrometry is presented. This temperature sensor is very small and flexible, allowing precise temperature measurements in a small and compact devices. Furthermore, it is unaffected by electric and magnetic field interferences making it ideal for monitoring permanent magnet temperatures in electric motors. Near-infrared grey body radiation spectrum from a neodymium magnet from was recorded and processed. The measured temperature range was from 30℃ to 80℃. The measurement technique was also tested under various repetition rate from 250㎐ to 250㎑. The results showed good temperature sensitivity and low temperature uncertainty of around ±1℃ at the fastest tested repetition rate.
장윤희 ( Yun Hee Chang ),서대훈 ( Dae Hoon Seo ),고아름 ( A Rum Koh ),김선영 ( Sun Young Kim ),임준만 ( Jun Man Lim ),한종섭 ( Jong Seup Han ),이상화 ( Sang Hwa Lee ),박선규 ( Sun Gyoo Park ),김양한 ( Yang Han Kim ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.2
화장품 사용 전후의 피부 상태는 주로 시각과 촉각 및 피부 구조에 기초한 정성적, 정량적 방법에 의해 평가되어 왔다. 본 연구에서는 화장품을 피부에 도포할 때 문지름으로 인한 마찰 소음이 발생한다는 사실을 기반으로, 피부 문지름에서 발생하는 소리를 측정하여 청각을 기반으로 하는 새로운 피부 상태 평가법을 개발하고자 하였다. 우선 무향 조건에서 고감도의 계측용 마이크로폰을 사용하여 피부를 문질렀을 때 발생하는 소리를 계측하였으며, 주파수 영역(1/3 옥타브 스펙트럼) 해석법을 이용하여 세정 전, 세정 후, 화장품 도포 후의 세 가지 피부 조건에 따른 소리의 스펙트럼 변화를 분석하였다. 그 결과, 세정 전보다 세정 후에 피부 문지름에서 발생하는 소리의 세기가 증가하는 경향을 보였으며, 화장품 도포 후에는 문지름 소음의 총 세기가 가장 작음을 확인하였다. 또한, 화장품 도포 후는 도포 전과 비교해볼 때, 2 kHz 미만의 저주파 대역의 에너지는 증가하며 반대로 2 kHz 이상의 고주파 영역의 에너지는 상대적으로 감소하는 변곡점이 발생하였다. 화장품 도포 후에 변곡점 근처에서 에너지 차이가 반전되는 이러한 현상은 피부의 물리적 모델인 풍선으로 해석되며, 저주파 영역에서의 소리 세기의 증가를 설명하기 위해 “유연함 지표”를 도입하고, 고주파 영역에서의 소리 세기의 감소를 설명하기 위해서는 “부드러움 지표”를 도입하였다. 그리고, 이러한 소리 측정을 통한 유연함 증가와 부드러움 증가는 각각 cutometer와 primos을 사용한 피부 유연함 측정과 피부 거칠기 측정 결과와 유사함을 확인하였다. 이상의 연구 결과를 통해 유연함과 부드러움 지표를 사용하는 청각에 기초한 평가 방법은 새로운 피부 상태 평가법으로 활용 가능할 것으로 판단된다. Efficacy of cosmetics has been mainly evaluated by qualitative and quantitative methods based on visual sense, tactile sense and skin structure until now. In this study, we suggested a novel evaluation method for skin status based on sound; measuring and analyzing the rubbing noise generated by applying cosmetics. First, the rubbing noise was measured at a close range by a high-sensitivity microphone in anechoic environment, and the noises were analyzed by 1/3 octave band analysis in frequency-domain. Three conditions, 1) before washing, 2) after washing and 3) after application of cosmetics, were compared. As a result, sound pressure level (SPL) of rubbing noise after washing was larger than that of before washing, and the SPL of rubbing noise after cosmetic application was the smallest. Furthermore, the energy of rubbing noise after application was higher than that of the before and after washing conditions in a low frequency band (lower than 2 kHz region). Conversely, the energy of rubbing noise after application was much lower than the others in a high-frequency band (upper than 2 kHz region). This change of energy distribution was described as a balloon-skin model. High SPL in the low frequency region after the cosmetic applications was due to the increase of “flexibility index”, while SPL in the high frequency region significantly decreased because of the attenuation which is related to “softness index”. Therefore, we developed two indices based on the spectrum-energy difference for evaluating skin conditions. This proposed method and indices were verified via skin flexibility and roughness measurement using cutometer and primos respectively. These results suggest that acoustic measurement of skin friction noise may be a new skin status evaluation method.