저강도 초음파 조사 부위의 골 소실 억제 효과 -골 밀도 및 형태학적 특성 평가

고창용,서동현,김효선,김한성,김상희,김진만,김기원,임도형,Ko, Chang-Yong,Seo, Dong-Hyun,Kim, Hyo-Seon,Kim, Han-Sung,Kim, Sang-Hee,Kim, Jin-Man,Kim, Ki-Won,Lim, Do-Hyung 대한의용생체공학회 2009 의공학회지 Vol.30 No.1

Purpose : The aim of this study is to evaluate a possibility of clinical application for the effects of low intensity ultrasound stimulation (LIUS) in morphological characteristics (i.e., structure, bone mineral density) of bone on osteoporotic fracturesprevention. Materials and Methods : Eight virgin 14-week-old ICR mice (approximate weight 25g) were used and ovariectomized (OVX) to induce osteoporosis. Right tibia (US) for each mouse served as the LIUS (1.5MHz frequency, 1.0 kHz pulse repetition on frequency, $30mW/cm^2$ intensity, $200{\mu}s$ pulse length, and stimulation for 20 minutes a day and 5 days a week over a 6-week period). Left tibia (CON) for each mouse served as the non-stimulated controls. Structural parameters and bone mineral density ($g/cm^3$) on trabecular bone of tibiae were calculated and measured from images derived in-vivo micro computed tomography (micro-CT) at 0 week and after 6weeks. Results : The BV/TV and Tb.N in US group were significantly bigger than those in CON group. The Tb.Pf in US group, moreover, was significantly smaller than that in CON group (p<0.05).For the others structural parameters and BMD, however, there were no significant difference between US group and CON group (p>0.05). Conclusion : The LIUS might prevent bone loss and keep bone connectivity in osteoporotic bones. Therefore, the LIUS might prevent effectively the osteoporotic fractures in clinics.

난소 절제술을 시행한 흰쥐 등뼈의 해면골과 피질골의 구조적 특성 추적관찰 및 기계적 특성 분석

고창용(Chang Yong Ko),우대곤(Dae Gon Woo),이태우(Tae Woo Lee),김한성(Han Sung Kim),이법이(Beob Yi Lee),탁계래(Gye Rae Tack) Korean Society for Precision Engineering 2007 한국정밀공학회지 Vol.24 No.1

전신진동이 골다공증이 유발된 쥐 해면골에 미치는 영향

고창용(Chang Yong Ko),이태우(Tae Woo Lee),우대곤(Dae Gon Woo),김효선(Hyo Seon Kim),김한성(Han Sung Kim),이법이(Beob Yi Lee),임도형(Dohyung Lim) Korean Society for Precision Engineering 2008 한국정밀공학회지 Vol.25 No.5

진동 피드백의 주파수가 근전도에 미치는 영향

고창용(Chang-Yong Ko),최부용(Bu-Yong Choi),김태형(Tae-Hyung Kim),허성필(Sung-Phil Heo) 한국정보기술학회 2023 Proceedings of KIIT Conference Vol.2023 No.11

임신기간 중 척추 해면골의 골질(bone qualities) 변화

고창용(Chang-Yong Ko),남궁범석(Bumseok Namgung),김효선(Hyo-seon Kim),김현동(Hyun Dong Kim),김한성(Han-Sung Kim) Korean Society for Precision Engineering 2010 한국정밀공학회지 Vol.27 No.4

생체 내 미세단층촬영 시스템을 이용한 C57BL/6 암컷 마우스의 복부 지방 평가 방법 연구

고창용 ( Chang Yong Ko ),서동현 ( Dong Hyun Seo ),김한성 ( Han Sung Kim ) 한국조직공학과 재생의학회 2010 조직공학과 재생의학 Vol.7 No.4

The aim of present study was to introduce the methodology for evaluation of abdominal adipose tissue using in-vivo micro-computed tomography (μ-CT). Eight C57BL/6 female mice (15 weeks, 21.5±1.3 g) were used. The torsos (the 1st lumbar vertebrae (L1) ~ the 6th lumbar vertebrae (L6) were scanned by using in-vivo μ-CT. The volumes of total (TV), visceral (VV) and subcutaneous (SV) abdominal adipose tissue were measured. The areas of total (TA), visceral (VA) and subcutaneous (SA) abdominal adipose tissue on proximal (P), distal (D) and intermediate (I) region in each lumbar vertebrae (total 18 regions) were measured. And then, the correlations between weight and volumes and between volumes and areas were analyzed. There were no significant correlations between weight and TV, VV and SV (p>0.05). Significant correlations between TV and VV and SV were shown (p<0.05). There were significant correlations between TV and TAs except on L2-I and between VV and VAs except on L1-P and L2-P (p<0.05). Significant correlations between SV and SA on L1-P, L1-D, L2-P and L6-I were shown (p<0.05). These results might indicate that TA and VA in L3~L6 predict TV and VV, whereas SA did not predict SV. In the conclusion, the methodology for evaluation of abdominal adipose tissue using in-vivo μ-CT might be useful.

전신진동이 난소절제술을 시행한 흰쥐 해면뼈에 미치는 영향

고창용(Chang-Yong Ko),이태우(Tae-Woo Lee),우대곤(Dae-Gon Woo),김효선(Hyo-Seon Kim),임도형(Dohyung Lim),김한성(Han-Sung Kim),이법이(Beob Yi Lee) 대한체질인류학회 2007 해부·생물인류학 (Anat Biol Anthropol) Vol.20 No.4

골다공증모델과 전신진동(whole body vibration)에 대한 많은 연구에 따르면, 전신진동이 뼈의 재흡수를 억제하거나 뼈의 생성에 도움을 준다고 보고되고 있다. 하지만 동일 개체에 대하여 전신진동의 골다공증 예방 및 치료 효과에 대해 추적한 연구는 거의 없는 실정이다. 이에 본 연구에서는 다양한 주파수의 전신진동을 난소적출술을 시행한 쥐에게 가한 후 시기별로 해면뼈의 형태학적 특성 변화에 대해 추적 관찰하여 전신진동의 골다공증 치료 효과에 대하여 연구하였다. 12마리의 암컷 SD (Sprague-Dawley) 흰쥐를 대조군(CON), 샴수술군(SHAM), 전신진동군(WBV 17, WBV 30, WBV 45)으로 나누었다. 골다공증을 유발하기 위하여 sham수술군을 제외한 모든 쥐에게 난소적출을 시행하였다. WBV군의 흰쥐에게 1㎜peak-peak의진폭과각각17 ㎐ (WBV 17), 30 ㎐ (WBV 30), 45 ㎐ (WBV 45)의주파수를갖는 전신진동을 1주일에 5일, 매일 30분씩 가하였다. 진동을 가하기 전과 가한지 8주 후에 쥐의 4번째 허리뼈를 Invivo Micro-CT를 이용하여 35 μm의 해상도로 촬영하였다. 이때 획득한 영상을 이용하여 구조적인 파라미터들을 구하고 해면뼈의 형태학적인 특징 변화에 대해 관찰하였다. 그 결과 WBV 45군의 뼈의 양적인 손실이 가장 적었으며 뼈잔기둥의 형태학적인 변화 역시 가장 적었고, WBV 17군은 대조군에 비하여 뼈잔기둥의 형태학적인 큰 변화는 없지만 뼈의 양적인 손실이 대조군에 비해서도 큰 편임을 알 수 있었다. 위의 결과로부터 적절한 진동은 뼈의 양적인 손실 및 구조적 변화를 방지할 수 있다는 것을 알 수 있었지만, 잘못된 전신진동은 뼈의 손실을 더 가속화시키는 것으로 사료된다. 그러므로 전신진동이 골다공증 치료의 새로운 방법으로 제시되어질 수 있다고 사료된다. Previous studies showed that whole body vibration could prevent bone resorption and stimulate new bone formation. The aim of this study is to detect and track effect of whole body vibration for osteoporotic bone of OVX rats. 12 Female rats were used and allocated into 5 group, CON, SHAM, WBV 17, WBV 30 and WBV 45. Rats except SHAM group were ovariectomised to induce osteoporosis. Rats in WBV groups were stimulated whole body vibration at magnitude of 1 ㎜peak-peak and frequency 17 ㎐, 30 ㎐ and 45 ㎐, repectively, for 8 weeks (20 min, 5 days/week). The 4th lumbar in rats were scanned at a resolution of 35 μm at baseline, before stimulation, and 8 weeks by In-vivo Micro-CT. To detect and track changes of morphological characteristics in lumbar trabecuar bone of rats, structural parameters were measured and calculated from acquiring images. In the results, changes of structural characteristics of WBV group were smaller than those of CON group. Loss of quantity of trabecular bone in WBV 45 was the least. In contrast, that in WBV 17 was the biggest. These results showed that reasonable whole body vibration beneficially affected osteoporotic bones. In addition to, whole body vibration was likely to be substituted partly for drug treatment.

무릎 신전 제한형 보조기 착용이 보행 시 하지 내 협응에 미치는 영향

장윤희(Yoonhee Chang),정보라(Bora Jeong),강성재(Sungjae Kang),류제청(Jeicheong Ryu),김규석(Gyu Seok Kim),문무성(Museong Mun),고창용(Chang-Yong Ko) 한국재활복지공학회 2016 재활복지공학회논문지 Vol.10 No.3

본 연구의 목적은 무릎 신전 제한형 보조기 착용 후 보행시 하지 내 협응에 미치는 영향을 평가하는 하는 것이다. 본 연구에는 7명의 건강한 성인 남성(32.3±2.7 세, 175.2±3.8cm, 76.2±8.7kg)이 참여하였다. 이들은 무릎보조기를 착용하거나 착용하지 않고 10m의 평지를 보행하였다. 이때 시공간적 파라미터, 하지의 각도, 하지 내 연속상대위상(CRP)를 측정 및 계산하였다. 그 결과 시공간적 요인은 차이가 없었다 (all p > 0.05). 무릎보조기 착용 시 엉덩관절의 가동범위 및 각도 변화는 없었으나, 무릎관절의 가동범위는 감소하였으며 (65.5±3.7° vs. 60.5±3.5°, p < 0.05), 최대 굴곡 역시 감소하였다 (입각기: 31.9±4.6 vs. 25.6±5.5, 유각기: 76.7±3.1 vs. 68.9±3.4, all p < 0.05). 발목관절의 가동범위는 변화가 없었으나, 최대 배측굴곡 (22.4±2.6 vs. 19.2±2.1, p< 0.05)은 감소하였으며, 최대 족저굴곡이 증가하였다 (9.5±3.0 vs. 15.7±2.2, p<0.05). 대부분의 CRP는 차이가 없었으며, 유각기 엉덩관절과 무릎관절 사이의 CRP만 감소하였다 (93.0±7.8 vs, 84.7±4.9, p < 0.05). CRP의 표준편차는 대부분 증가하였다 (유각기 엉덩관절과 무릎관절사이: 25.1±6.7 vs. 32.4±1.9, 입각기 무릎관절과 발목관절사이: 46.0±12.9 vs. 80.1±31.1, 유각기 무릎관절과 발목관절 사이: 34.5±4.1 vs. 37.6± 3.1, all p < 0.05). 이와 같은 결과로부터 무릎 신전 제한형 보조기 착용은 관절의 각도, 하지 내 협응에는 영향을 미치지만, 보행특성에는 큰 영향을 미치는 않는다는 것을 확인하였다. The aim of this study was to evaluate Influence on intra-limb coordination in individuals wearing knee brace during walking. Seven healthy male adults (32.3±2.7 years old, 175.2±3.8cm, 76.2±8.7kg) participated. They wore knee brace or didn`t wear any knee brace and were asked to walk along a 10 m long walkway. Spatiotemporal parameters, angles of the lower limbs, and intra-limb continuous relative phase (CRP) were measured and calculated. No differences of spatiotemporal parameters were shown (all p > 0.05). There were no changes in the angle and its range of motion (ROM) in the hip for the subjects as wearing knee brace, while ROM (65.5±3.7° vs. 60.5±3.5°, p < 0.05) of the angle and maximum flexion angles (stance: 31.9±4.6 vs. 25.6±5.5, swing: 76.7±3.1 vs. 68.9±3.4, all p < 0.05) in the knee significantly decreased. No changes in ROM of angle in the ankle were shown, whereas maximum dorsiflexion decreased (22.4±2.6 vs. 19.2±2.1, p< 0.05) and maximum plantarflexion increased (9.5±3.0 vs. 15.7±2.2, p<0.05). There were no changes in most of CRP between joints. CRP between the hip and knee joints decreased (93.0±7.8 vs, 84.7±4.9, p < 0.05). Most of CRP standard deviation increased (between the hip and ankle joint during swing: 25.1±6.7 vs. 32.4±1.9, between the knee and ankle joint during stance: 46.0±12.9 vs. 80.1±31.1, between the knee and ankle joint during swing: 34.5±4.1 vs. 37.6±3.1, all p < 0.05). These results indicated that wearing knee brace affected joint angle and intra-limb coordination, but less affected gait features.

수입 관상어에서 분리한 motile aeromonads의 특성

진세윤,고창용,이예지,정윤희,주성철,김은희,Jin, Se-yun,Ko, Chang-yong,Lee, Ye-ji,Jung, Yun-hee,Ju, Seong-cheol,Kim, Eunheui 한국어병학회 2017 한국어병학회지 Vol.30 No.2

현재 국내에 유통되고 있는 담수관상어의 대부분은 수입에 의존하므로 외래 병원체 및 항생제 내성균의 유입 가능성이 크다. 본 연구에서는 담수에 상재하는 균으로 인수공통 감염이 가능하며 어류에 조건성병원균으로 작용하는 Aeromonas hydrophila를 다양한 수입 담수관상어로부터 분리하여 특성을 분석하고자 하였다. 수입 후 1일 이내의 관상어를 수족관으로부터 수집하여 0.5% NaCl을 포함하는 TSA 배지에 간, 신장, 비장을 도말하여 세균을 분리하였다. 세균의 형태, 생화학적 특성 및 starch- ampicillin agar (SA)에서의 clear zone 형성 유무에 근거하여 분리 균의 약 70%인 226 균주가 Aeromonas spp.로 확인되어 이들의 분리 빈도가 매우 높음을 알 수 있었다. 이들 중 9균주를 선택하여 16S rRNA gene의 sequence를 분석한 결과 모두 A. hydrophila로 동정되었으며 API 20E test에서 대부분의 분리 균주는 amygdalin, D-melibiose, D-sucrose, trisodium citrate, L-arabinose 이용에서 표준 균주와 차이를 보였다. 10종의 항균제에 대한 분리 균주의 감수성을 디스크 확산법으로 확인한 결과 amoxycillin, ampicillin에 대해서는 모든 균주가 저항성이었고 florfecicol에 대해서는 모두 감수성이었다. 그러나 7균주는 tetracycline 계통의 항균제와 erythromycin, nalidixic acid 등에 복합내성을 보였다. A. hydrophila로 동정된 9개 분리 균주를 금붕어에 인위 감염을 실시한 결과, 3 균주는 5일 이내에 60~80%의 폐사를 나타내었다. 이러한 결과는 다약제내성이면서 독력이 있는 Aeromonas가 수입관 상어를 따라 국내로 유입되어 전파될 수 있으므로 수입상에서 소비처로 판매되기 전의 방어적 조치가 필요하다는 것을 시사한다. The majority of freshwater ornamental fish are imported and distributed domestically, causing high risk of exposure to exotic pathogens and drug resistant bacteria in Korea. Aeromonas hydrophila is known as a common species of fresh water bacteria and opportunistic fish pathogen, as well as a species causing zoonotic infection. In this study, we isolated motile aeromonads from various imported freshwater ornamental fish and studied the characters of the isolates. Imported freshwater ornamental fish were purchased on day 1 after the fish were deposited in the aquarium. Bacteria were isolated from the liver, kidney and spleen of fish using 0.5% NaCl containing tryptic soy agar medium. Bacteria were grouped on the basis of their morphological characteristics. The colonies with clear zone on starch-ampicillin agar (SA agar) were tentatively identified as Aeromonas spp. Two hundred and twenty-six strains, about 70% of total isolates were assumed to be Aeromonas spp. Nine isolates were further identified based on the result of the API 20E test and PCR using primers specific for A. hydrophila 16S rRNA gene. The isolates were identified as A. hydrophila and the API 20E test showed differences in trisodium citrate, D-sucrose, D-melibiose, amygdalin and L-arabinose availability between the nine isolates and standard A. hydrophila. The susceptibilities of the isolated bacteria to 10 antibacterial agents were confirmed by the disk diffusion method. Isolated strains were found to be resistant to amoxicillin and ampicillin and sensitive to florfenicol. However, 7 isolates showed multiple drug resistances to erythromycin, oxytetracycline, nalidixic acid etc. Pathogenicity of the isolates was determined by the artificial challenge test on goldfish (Carassius auratus). Three isolates caused 60 ~ 80% mortality in goldfish within 5 days after the initiation of challenge. These results indicate that multiple drug resistant, highly pathogenic and exotic A. hydrophila can spread to domestic aquarium and the preventive treatment of fish before sale is necessary.

전이성 골암에 의한 해면골의 미세구조와 골화 분포 변화

박선욱,전옥희,고창용,김지현,김한성,전경진,임도형,Park, Sun-Wook,Jeon, Ok-Hee,Ko, Chang-Yong,Kim, Chi-Hyun,Kim, Han-Sung,Chun, Keyoung-Jin,Lim, Do-Hyung 대한의용생체공학회 2009 의공학회지 Vol.30 No.5

Purpose: The aim of present study is to detect longitudinal alterations of mechanical characteristic determined by bone quality (microarchitecture and degree of mineralization) on femur trabecular bone due to metastatic bone tumor Materials and Methods: Each 6 female SD rats (12 weeks old, approximate 250g) were allocated in SHAM and TUMOR Group. W256 (Walker carcinosarcoma 256 malignant breast cancer cell) was injected into the right femur (intraosseous injection) in TUMOR Group, whereas 0.9% NaCl (saline solution) was injected in SHAM Group. The right hind limbs of all rats were scanned by in-vivo micro-CT to acquire structural parameters, bone mineral density, X-ray attenuation and bone mineralization distribution at 0 week and 4 weeks after surgery. Results: BMD, BV/TV and Tb.N of trabecular bone in TUMOR group were markedly decreased (26%, 11% and 23%) while those in SHAM group were significantly increased (34%, 48% and 11%) (p<0.05). BS/BV, Tb.Sp and SMI in TUMOR group were significantly increased (-16%, 38% and 2%) compared with those in SHAM group (-33%, 12% and -16%) (p<0.05). Additionally, bone mineralization in TUMOR group significantly decreased while those in SHAM group was significantly increased (p<0.05). Conclusion: It is identified that how much bone microarchitecture and mineralization are diminished due to the metastatic bone tumor. The results may be helpful to prediction of fracture risk by metastatic bone tumor.