포공영(蒲公英)이 MC/9 mast cell에서의 Th2 cytokine 발현에 미치는 영향

장문희,최재송,배나영,안택원,Jang, Moon-Hee,Choi, Jae-Song,Bae, Na-Young,Ahn, Teak-Won 사상체질의학회 2012 사상체질의학회지 Vol.24 No.1

1. Objective : The purpose of this study is to investigate the effects of TAM (Taraxacum mongolicum) on Th2 cytokine production in MC/9 mast cells. 2. Methods : The effects of TAM was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. 3. Results : 1) TAM inhibited the IL-4 production significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$, $200{\mu}g/ml$. 2) TAM inhibited the IL-13 production significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$, $200{\mu}g/ml$. 3) TAM inhibited the IL-4 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 4) TAM inhibited the IL-5 mRNA expression significantly in comparison to PI-control group at concentration of $50{\mu}g/ml$, $100{\mu}g/ml$. 5) TAM inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 6) TAM inhibited the IL-13 mRNA expression significantly in comparison to PI-control group at concentration of $100{\mu}g/ml$. 4. Conclusions : These results indicate that TAM (Taraxacum mongolicum) has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

아토피 피부염 유발 NC/Nga mouse에서 도고 온천수 유황 성분의 효과

장문희,고가연,안요찬,안택원,Jang, Moon-Hee,Go, Ga-Yeon,Ahn, Yo-Chan,Ahn, Taek-Won 대한한방안이비인후피부과학회 2013 한방안이비인후피부과학회지 Vol.26 No.3

Objectives : The purpose of this study is to investigate the effects of DOGO phreatic water containing sulphur on Atopic Dermatitis in NC/Nga mouse. Methods : We made DOGO phreatic water removed sulphur using Twin Alternating Sulfate Eater. After making atopic dermatitis caused by sensitizing NC/Nga mouse to DNCB(dinitrochlorobenzene), we made mouse swim in tanks each filled with distilled water, tap water, DOGO phreatic water(contain sulphur), DOGO phreatic water(remove sulphur) for 30minutes everyday. 3weeks later, we analyzed skin clinical score, total IgE levels(by ELISA), WBC differential counting(Neutrophils, Monocytes), absolute cell number of $Neutrophil^+Gr-1^+$, CCR3 mRNA expressions(by Real-time PCR), IL-4, IFN-${\gamma}$ production levels(by ELISA), histologic test(by H&E staining, toluidine blue staining). Results : The results of making NC/Nga mouse induced atopic dermatitis swim in tanks filled with DOGO phreatic water(contain sulphur) are as follows. 1. Skin clinical scores were decreased significantly in comparison to control group. 2. Total IgG levels were decreased significantly in comparison to control group. 3. WBC differential counting(Neutrophils, Monocytes) were decreased significantly in c.mparison to control group. 4. Absolute cell number of $Neutrophil^+Gr-1^+$ were decreased significantly in comparison to control group. 5. CCR3 mRNA expressions were decreased significantly in comparison to control group. 6. IL-4, IFN-${\gamma}$ production levels were decreased significantly in comparison to control group. 7. The epithelial tissue thickness, leucocytes infiltration, erythema, edema, excoriation, scaling, mast cells infiltrations in dorsal skin were decreased in comparison to control group. Conclusions : These results indicate that DOGO phreatic water(contain sulphur) can be used for helping treat atopic dermatitis.

한방 입욕제 조성물이 MC/9 mast cell에서의 Th2 cytokine 발현에 미치는 영향

장문희,최재송,최애련,안택원,Jang, Moon-Hee,Choi, Jae-Song,Choi, Ae-Ryun,Ahn, Taek-Won 사상체질의학회 2012 사상체질의학회지 Vol.24 No.3

Objectives The purpose of this study is to investigate the effects of korean herbal bathing extracts composition 1 and composition 2 on Th2 cytokine production in MC/9 mast cells. Methods The effects of composition 1, 2 was analyzed by ELISA and Real-time PCR in MC/9 mast cells. Levels of IL-5, IL-13 were measured using enzyme-linked immunosorbent assays(ELISA). mRNA levels of IL-4, IL-5, IL-6, IL-13 were analyzed with Real-time PCR. Results Composition 1, 2 inhibited the IL-5, IL-13 production significantly(p<.001) in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Composition 1, 2 inhibited the IL-4, IL-5, IL-13 mRNA expression significantly in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Composition 1 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 200 ${\mu}g/ml$. Composition 2 inhibited the IL-6 mRNA expression significantly in comparison to PI-control group at concentration of 100 ${\mu}g/mL$, 200 ${\mu}g/mL$. Conclusions These results indicate that composition 1, 2 has the effect of decreasing the Th2 cytokine production in the MC/9 mast cell.

혈관성 치매 환자 치험 3례

장문희,최재송,배나영,안택원,Jang, Moon-Hee,Choi, Jae-Song,Bae, Na-Young,Ahn, Teak-Won 사상체질의학회 2011 사상체질의학회지 Vol.23 No.3

1. Objectives: This study is about three vascular dementia patients with disorientation, memory impairment and cognitive disorder. In this study, we evaluated the effects of Sasang constitutional treatment and suggested using the Mini Mental Status Examination - Korea (MMSE-K), Global Deterioration Scale (GDS). 2. Methods: These three patients were treated by Sasang constitutional herbal medications based on "Donguisusebowon". Acupuncture and west medications were limited as much as possible. We evaluated the symptoms through the Mini Mental Status Examination - Korea (MMSE-K), GDS (Global Deterioration Scale). 3. Results and Conclusions: Disorientation, memory impairment and cognitive disorder that were these three patients chief complaints were improved. This case study shows that Sasang constitutional herbal medications are an effective treatment for vascular dementia patients.

중년여성의 체형별 프린세스라인 토르소 원형 개발

장문희 ( Moon Hee Jang ),양정은 ( Chung Eun Yang ) 한국의류산업학회 2014 한국의류산업학회지 Vol.16 No.2

This study developed and proposed a pattern that can fit the body and compensate for the defects of the body type by applying shoulder princess line to the torso pattern and including formative characteristics of each object on the study of body shape`s change in 40-59 year old middle aged women. The results of this study are as follows; First, according to the analysis of ``Size Korea 2010`` such as average, standard deviation, minimum value, and maximum value of 48items, women showed increase in most of the items except height as they became aged. Second, factor analysis was made to understand the shape component factors of middle aged women and to use them for the body type classification. Third, cluster analysis was made according to the shape of front and sides which should be considered in pattern production based on the factor analysis results, and the body type with the measurement values most similar to the average of direct measurement of ‘Size Korea 2010`` was set as standard. Fourth, in designing torso patterns through the 1st and the 2ndwearing experiments according to the body type, body shapes such as Chest Circumference, Waist Circumference, Hip Circumference, and Waist Back Length were considered in pattern design, goodness-of-fit was enhanced with difference in margin according to body type and different margins in front and back, and fitting satisfaction was improved by applying princess line.

한국인과 중국인의 황사로 인한 자각증상 및 예방행동

장문희(Moon-Hee Chang),하은희(Eun-Hee Ha),서영주(Young-Ju Suh),이보은(Bo-Eun Lee),권호장(Ho-Jang Kwon),황승식(Seong-Sik Hwang),이충민(Zhong-Min Li),서주희(Ju-Hee Seo),김병미(Byung-Mi Kim) 한국대기환경학회 2009 한국대기환경학회지 Vol.25 No.1

용육조위탕(龍肉調胃湯) 증류액이 노화 흰쥐 비장 세포의 면역활성에 미치는 영향

이진욱,장문희,최재송,안택원,Yi, Jin-Wook,Jang, Moon-Hee,Choi, Jae-Song,Ahn, Taek-Won 사상체질의학회 2013 사상체질의학회지 Vol.25 No.3

Objectives The purpose of this study was to investigate the effect of Yongyukjowitang(YJT) distillate on the immune activity of spleen cells of aged SD rats. Methods Spleen cells from 10w, 52w, 72w old rats were stimulated with Concanavalin A(Con A) and treated with Vitamin C(Vit.C) or Yongyukjowitang distillate(YJT). After 24 hours, levels of IL-2, IL-4, IL-10, IFN-${\gamma}$ were measured using ELISA in spleen cells. Results and Conclusions 1. The concentration of IL-2, IL-4 in spleen cells of 52 weeks old SD rats(YJT group) significantly decreased in comparison with ConA group. 2. The concentration of IFN-${\gamma}$ in spleen cells of 72 weeks old SD rats significantly increased in comparison with 10 weeks old SD rats(NT group), but decreased in comparison with 10 weeks old SD rats(YJT group). These results suggest that Yongyukjowitang distillate has an effect on the immune activity of spleen cells of aged SD rats.

열다한소탕가감방(熱多寒少湯加減方)으로 호전된 파킨슨병 환자 1례

최재송,장문희,안택원,Choi, Jae-Song,Jang, Moon-Hee,Ahn, Taek-Won 사상체질의학회 2012 사상체질의학회지 Vol.24 No.3

Objectives This study is about a "Taeumin" parkinson's disease patient with bradykinesia, tremor, weakness and dysautonomia etc. In this case, we evaluated the effect of Yuldahanso-tang gagam for parkinson's disease. Methods This patient was treated by Sasang constitutional herbal medication based on "Donguisusebowon". Acupuncture and west medications were used together. We evaluated the clinical progress through two methods, DITI(Digital Infrared Thermal Imaging) and VAS(Visual Analogue Scale). Results During 11months treatment, chief complaints and symptoms of this patient were improved. Conclusions This study shows that Yuldahanso-tang gagam is an effective treatment for "Taeumin" parkinson's disease patients.

형방지황탕(荊防地黃湯)으로 호전된 소양인(少陽人) 소뇌위축증 환자의 현훈(眩暈) 치험 1례

고가연,장문희,김강윤,안택원,Go, Ga-Yeon,Jang, Moon-Hee,Kim, Kang-Yoon,Ahn, Taek-Won 사상체질의학회 2013 사상체질의학회지 Vol.25 No.3

Objectives This case is about a Soyangin cerebellar atrophy patient. In this study, we report the effect of Sasang Constitutional treatment to this patient. Methods This patient was treated by Soyangin's constitutional medications and acupuncture according to the result of Sasang Constitutional diagnosis. We used Visual Analogue Scale(VAS) and Equilibrium Function Test. Results and Conclusions This patient's chief complaints were dizziness. By using Hyeungbangjihwang-tang, she showed positive response about her symptom. This study shows that Hyeungbangjihwang-tang has effect to cerebellar atrophy in Soyangin.

열다한소탕(熱多寒少湯) 가감방(加減方)의 자가탐식(自家貪食) 유도 활성과 관련 단백질 탐색

김희주,배나영,장문희,양현옥,안택원,Kim, Hee-Ju,Bae, Na-Young,Jang, Moon-Hee,Yang, Hyun-Ok,Ahn, Taek-Won 사상체질의학회 2013 사상체질의학회지 Vol.25 No.3

Objectives Modified Yeolda-Hanso tang (MYH) is a traditional herbal formula in Korea for various diseases. MYH is containing the 10 herbs : Pueraria lobata (Willd.) Ohwi, Angelica tenuissima Nakai, Scutellaria baicalensis Georgi, Platycodon grandiflorum (Jacq), Angelicae Dahurica, Cimicifuga heracleifolia Kom, Raphanus sativa L., Polygala tenuifolia (Willd), Acorus gramineus Soland and Dimocarpus longan Lour. The 10 herbs is constituted as a ratio of the 6:4:2:1:2:2:2:4:6:6. We investigated neuroprotective effects of MYH on human neuroblastoma SH-SY5Y cells and evaluated the ability of MYH to prevent and treat for neurodegenerative diseases such as Parkinson's disease via basal autophagy enhancement. Methods Pharmacological induction of Autophagy by MYH in SH-SY5Y cells: Induction of autophagy by MYH in human neuroblastoma SH-SY5Y cells was carreid out by immunoblot analysis with several autophagy markers. SH-SY5Y cells were treated with MYH at the concentration of 400 and $800{\mu}g/ml$ for 24 hr. Specifically, the autophagosome proteins LC3 II and Atg5 levels were increased and autophagy pathway related proteins such as beclin-1, PI3 Kinase class III protein, ULK1, mTOR and AMPK were activated. Conclusions MYH can enhance the induction of autophagy through key regulator AMPK, mTOR, and Beclin-1 and it should be considered as a possible candidate of neuroprotective agents for such as Parkinson's disease.