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흰쥐 폐에서 Prednisone 에 의한 산소 독성과 Liposomal Superoxide Dismutase 의 보호 효과
김수영,백광진,이희성 ( Soo Young Kim,Kwang Jin Baek,Hee Sung Lee ) 생화학분자생물학회 1990 BMB Reports Vol.23 No.3
The changes of superoxide dismutase (SOD), catalase, peroxidase and xanthine oxidase activities in the lung were observed after treating rats either with prednisone (10 ㎎/100 g body weight, group A) alone or with oral liposomal superoxide dismutase (3,000 units) followed by prednisone (10 ㎎/100 g body weight, group B). The results were summarized as follows: 1. In group A, Cu, Zn-SOD activity was higher than non-treated control values, but group B showed lower than that in the control. 2. The activity of Mn-SOD in group A increased progressively until the 3rd day after prednisone treatment and then decreased thereafter, but in group B, the activity was not changed except on the 4th day after the treatment. 3. In all experimental groups, the catalase activity was higher than that in the control, and gorup B showed higher activity than that in group A except on the 4th day after the treatment. 4. The activity of peroxidase in group A was higher than that in the control, but the activity in group B was similar to that in control. 5. In group A, xanthine oxidase activity showed higher than that in the control and group B except on the 1st day after the treatment, and the activity in group A increased with time. The above results suggest that prednisone administration could enhance the xanthine oxidase activity which lead to the production of oxygen radicals in the lung, and that the liposomal SOD may effectively protect the lung from the oxygen toxicity.
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흰쥐 폐에서 Prednisone에 의한 산소 독성과 Liposomal Superoxide Dismutase의 보호 효과
김수영,백광진,이희성,Kim, Soo-Young,Baek, Kwang-Jin,Lee, Hee-Sung 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.3
경구투여 방법으로 흰쥐에 prednisone(10mg/100g body weight)을 투여하거나(A 실험군) liposomal SOD(3,000 units) 투여 후 prednisone(10mg/l00g body weight)을 투여하여 (B 실험군) 1-4 및 5일 뒤에 폐를 적출해서 Cu, Zn-SOD, Mn-SOD, catalase, peroxidase, xanthine oxidase 등의 활성도 변화를 관찰하여 다음과 같은 결과를 얻었다. 1. Cu, Zn-SOD 활성도는, 대부분의 A 실험군이 대조군(생리 식염수만을 1ml/100g body weight 용량으로 경구투여)보다 증가했으나, B 실험군은 대조군보다 감소했다. 2. Mn-SOD 활성변화에서 A 실험군은 3일까지 활성이 점차 증가했으나 그 뒤에 활성이 감소했으며, B 실험군은 4일군만 제외하고 대조군과 비슷한 활성을 보였다. 3 모든 실험군에서 catalase의 활성도는 대조군보다 높았으며, 4일 군만 제외하고 B 실험군의 활성이 A 실험군보다 높았다. 4. Peroxidase의 활성변화에서, A 실험군 모두는 대조군보다 활성이 유의하게 증가한 반면, B 실험군 모두는 대조군과 비슷한 수준의 활성을 나타냈다. 5. Xanthine oxidase의 활성변화에서, A 실험군은 1일 군만 제외하고 대조군에 비해 활성이 유의하게 증가했으며, B 실험군과의 비교에서도 1일 군만 제외하고 활성이 항상 높았다. 또한 시간경과에 따라서도 활성이 증가하는 양상을 보였다. 이상과 같이 prednisone의 경구투여에 의해 xanthine oxidase의 활성이 증가되므로 superoxide radical 등 oxygen radical이 생성되어 폐에서 산소 독성 효과가 있으리라 생각하며, SOD, catalase, peroxidase 등 oxygen radical 제거에 관여하는 효소들은 활성이 증가하여 폐에서의 산소 독성 방어기전에 중요한 역할을 하는 것으로 생각한다. The changes of superoxide dismutase (SOD), catalase, peroxidase and xanthine oxidase activities in the lung were observed after treating rats either with prednisone (10 mg/100 g body weight, group A) alone or with oral liposomal superoxide dismutase (3,000 units) followed by prednisone (10 mg/100 g body weight, group B). The results were summarized as follows: 1. In group A, Cu, Zn-SOD activity was higher than non-treated control values, but group B showed lower than that in the control. 2. The activity of Mn-SOD in group A increased progressively until the 3rd day after prednisone treatment and then decreased thereafter, but in group B, the activity was not changed except on the 4th day after the treatment. 3. In all experimental groups, the catalase activity was higher than that in the control, and gorup B showed higher activity than that in group A except on the 4th day after the treatment. 4. The activity of peroxidase in group A was higher than that in the control, but the activity in group B was similar to that in control. 5. In group A, xanthine oxidase activity showed higher than that in the control and group B except on the 1st day after the treatment, and the activity in group A increased with time. The above results suggest that prednisone administration could enhance the xanthine oxidase activity which lead to the production of oxygen radicals in the lung, and that the liposomal SOD may effectively protect the lung from the oxygen toxicity.
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엄범규(Eom Beom-Gyu),박광수(Park Kwang-Soo),이영엽(Lee Young-Yeob),김재철(Kim Jae-Chul),이희성(Lee Hi Sung) 한국철도학회 2009 한국철도학회 학술발표대회논문집 Vol.2009 No.11월
With increasing international interest in further environmental problems and energy, prospects are that interest in railroad is increased emerging the importance of railroad which has high energy efficiency. In addition, a rate of sharing freight of railroad is expected to be increased gradually because existing line became under freight-based operation and investment in railroad was expanded since the completion of Kyoung-bu new line. Therefore, research of Korean DMT freight car is required in accordance with these increase in a rate of sharing freight of railroad. In this paper, we have tested parts and systems necessary for the basic design and developed analysis model combined with those components in order to ensure running safety of Korean DMT freight car on existing line. Especially, we analyzed the running performance of the Korean DMT freight car passing through the curve by its vibration and transmission process. In results of the running performance analyzation at curve section, the right & left acceleration of the vibration which is delivered from wheelset to the carbody appeared most greatly at all positions & systems. And the vibration which is delivered to wheelset→bogie→carbody is decreased gradually. Also the up & down vibration due to the decrease in transmission process appeared most greatly.
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Kim, Tae-Woon,Choi, Chang-Young,Kim, Seong Won,Lee, Gwang-Hoon,Baek, Kwang Jin,Kwon, Nyoun Soo,Lee, Hee Sung,Yun, Hye-Young 중앙대학교 의과대학 의과학연구소 2000 中央醫大誌 Vol.25 No.1
신경 활성이 발생 및 세포 생존 유지에 중요한 조절인자라는 사실은 신경계의 활성 의존성 현상에 대한 그 동안의 연구결과를 통해 밝혀졌다. 특히, 염화칼륨(KCI)의 처리에 의한 세포막의 탈분극은 여러 종류의 일차배양 신경세포 및 세포주에서 혈청 존재시 생존을 증가시키고, 혈청 제거 시 유발되는 세포사멸을 현저히 저해시킨다. 그러나, 막 탈분극이 세포 생존 유지에 어떻게 관여하는지에 대해서는 아직까지 분명히 밝혀져 있지 않다. 본 연구에서는 막 탈분극에 의해 유입된 칼슘 이온이 어떻게 신경성장인자(NGF)가 제거된 PC12 신경세포주의 생존을 증가시키는지는 이해하기 위해 생존유지에 관련이 있을 것으로 추정되는 세포 내부 신호 전달 물질을 조사였다. L-type voltage-sensitive calium channels(VSCCs), 외부 칼슘의 유입, ras, extracellular single-regulated kinase(Erk),phosphatidylinositol-3 kinase(PI3K)의 활성 억제제를 각각 분화된 지 10일이 된PC12 신경 세포주에 투여하여 세포 생존유지에 미치는 효과를 측정한 결과 모두 세포막 탈분극에 의해 촉진되는 세포 생존을 억제했다. 또한, 전사 억제제와 단백질 합성 억제제를 전처리한 경우에서도 같은 효과가 나타났다. 이 결과는 L-type VSCCs를 통한 외부 칼슘의 세포내 유입, ras와Erk,PI3K같은 ras의 하위 효과자들, 그리고 새로운 유전자 전사 및 단백질의 합성이 PC12 세포의 생존유지에 매우 중요한 역할을 수행함을 암시한다. 따라서, 칼슘,ras,Erk,PI3K가 관여하는 신호 전달 경로가 세포핵으로 신호를 전달하여 아직 알려지지 않은 유전자의 전사활성 및 단백질 발현을 유도하여 세포막 탈분극에 의해 촉진되는 세포 생존유지를 조절하는 것으로 추정된다.
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김수영,이희성,권년수 중앙대학교 의과대학 의과학연구소 1993 中央醫大誌 Vol.18 No.2
In mammals, nitric oxide (NO) plays important roles in many physiological function such as endothelium-dependent vascular relaxation, non-adrenergic non-cholinergic neurotransmission. some cytotoxic actions of activated macrophages are also mediated by NO. Growth-inhibitory effects of macrophage-derived NO have been known in tumor cells, intracellular tropozoites, fungi, intracellular mycobacteriu, and lympho cytes. In the present study anti-bacterial effects of No were investigated. S-Nitroso-N-acetyl-D, L-penicillamine (SNAP) was used to produce NO insolution. SNAP at the concentration of 5mM inhibited the growth of Staphylococcus aureus, Bacillus anthracis and Escherichia coli in nutrient broths by 80, 95 and 60% respectively, in 3 h incubation at 37℃ . The growth of Salmonella paratyphi B was completely inhibited by the same concentration of SNAP. SNAP is decomposed to NO and N-acetyl-D, L-penicillamine during the incubation in an aqueous solution. NO rapidly reacts with itself, oxygen and water to generate accumulating endproducts, nitrite and nitrate. Neither sodium nitrite nor N-acetyl-D, L-penicillamine at the concentration of 5 mM wasgrowth-inhibitory. These results indicate that NO produced from SNAP inhibits bacterial growth. The growth inhibitory effect of SNAP was partially reduced by addition of deoxyribonucleosides, which can be converted to deoxyribonucleotide in the obsence of ribonucleotide reductase. Therefore, the inhibitory effects of NO on bacterial growth may due to the deactivation of ribonucleotide reductase.
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정상 및 Phenylhydrazine을 처리한 토끼 혈장의 Isocitrate Dehydrogenase 활성도에 관한 연구
김영수,이동욱,이희성 중앙대학교 의과대학 의과학연구소 1983 中央醫大誌 Vol.8 No.1
In order to establish the general pattern of synthesis and degradation of plasma isocitrate dehydrogenase(isocitrate: NADP oxidoreductase: EC 1.1.1.42) in normal and reticulocytosic domestic rabbits, sequential measurements of activity of isocitrate dehydrogenase in the plasma of normal and phenylhydrazine treated rabbits were conducted. The activity of isocitrate dehydrogenase was measured by the method of King. The results are summarized as follows ; 1. The reticulocyte count of normal rabbit blood was approximately 2.5%, and the administration of phenylhydrazine resulted in rapid and profound reticulocytosis, i.e., about 63.5% on day 6 and essentially 100% on day 7. 2. The activity of plasma isocitrate dehydrogenase in normal blood was 0.23 unit/ml plasma. 3. In phenylhydrazine treated rabbits, isocitrate dehydiogenase activity was maximal on day 5, about 0.59 unit/ml plasma, which is about 2.3 fold higher than the normal value(K=0.0183; K_d=0.0589; t1/2=17.81 hrs). 4. The rate constants following recovery from phenylhydrazine treatment were K_s=0.0060; K_4 -0.0273 and t1/2=25.38 hrs.
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Lipopolysaccharide, Dexamethasone, 및 N-Nitro-L-Arginine Methyl Ester가 흰쥐 간 조직의 프리라디칼 발생과 제거에 미치는 영향
한기정,김석배,김양균,윤혜영,백광진,이희성,권년수 중앙대학교 의과대학 의과학연구소 2003 中央醫大誌 Vol.28 No.1
Lipopolysaccharide (LPS) stimulates various immune and inflammatory reactions to induces the generation of reactive oxygen intermediates (ROI) and reactive nitrogen intermediates (RNI). ROI and RNI are free radicals with very high reactivity, oxidize cellular constituents including proteins and lipid membranes, and thus play important roles in the pathogenesis of tissue damages in LPS-induced septic shock. The present study examined effects of dexamethasone (DexM), and N-nitro-L-arginine methyl ester (L-NAME) on the generation of ROI and RNI, and on the activities of enzymes removing ROI in the liver of rat model of septic shock. DexM has widely been used as an immunosuppressant. L-NAME has known to block synthesis of nitric oxide (NO), the main RNI, by inhibiting NO synthase (NOS). LPS (10 mg/kg body weight) markedly enhanced NO generation in serum and liver. The increased NO generation was completely blocked by pretreatment with DexM (10 mg/kg) and effectively inhibited by L-NAME (10 mg/kg). Induction of NOS protein expression by LPS was demonstrated by a Western analysis. The NOS expression was completely blocked by DexM pre-treatment, and markedly inhibited by L-NAME. However, the generation of superoxide radical, an important ROI, was not enhance in rat liver by LPS, rather it was decreased a little. Superoxide radical production was increased by L-NAME treatment or combined treatment of DexM and LPS. The level of lipid peroxidation, an index for free radical-induced cell damage, was increased by either LPS or DexM. The enhancement of lipid peroxidation by DexM was abolished by adding LPS. DexM markedly enhanced the activities of ROI removing enzymes, superoxide dismutase, glutathione peroxidase and catalase. However, rats were treated with LPS in addition to DexM, the elevated activities of superoxide dismutase, glutathione peroxidase and catalase were abolished. These results provide information on the generation and removal of ROI and RNI by LPS, DexM and L-NAME in rat liver, and on development of drugs to inhibit free radical-induced liver damage in sepsis.
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