Genome sequence of Veillonella atypica KHUD-V1 isolated from a human subgingival dental plaque of periodontitis lesion

이재형,신승윤,이한,양석빈,장은영,류재인,이진용,문지회,Lee, Jae-Hyung,Shin, Seung-Yun,Lee, Han,Yang, Seok Bin,Jang, Eun-Young,Ryu, Jae-In,Lee, Jin-Yong,Moon, Ji-Hoi The Microbiological Society of Korea 2019 미생물학회지 Vol.55 No.1

본 논문에서는 한국인 만성 치주염 환자의 치은연하치태에서 분리된 Veillonella atypica KHUD-V1의 유전체 서열을 보고한다. 다른 V. atypica 균주와 달리, KHUD-V1에서는 프로 파지 및 이와 관련된 것으로 추정되는 여러 병독성 인자가 확인되었다. V. atypica KHUD-V1 균주 및 이 균주의 유전체 서열정보는 Veillonella의 유전체 다양성을 진화론적 관점에서 이해하고, V. atypica의 병독성 및 유전적 다양성에 기여하는 프로파지의 역할을 연구하는데 유용할 것이다. Here we report the genome sequence of Veillonella atypica strain KHUD-V1 isolated from subgingival dental plaque of Korean chronic periodontitis patients. Unlike other V. atypica strains, KHUD-V1 carries two prophage regions and prophage remnants, as well as several genes homologous to prophage-associated virulence factors, such as virulence-associated protein E, a Clp protease, and a toxin-antitoxin system. The isolate and its genome sequence obtained here will aid to understand the diversity of the genome architecture of Veillonella within an evolutionary framework and the role of prophages that contribute to the genetic diversity as well as the virulence of V. atypica.

설하 신경 마비로 인한 설마비 환자 치험 1례

이재형,한성준,이엄지,이정은,남성욱,하나연,김진성,Lee, Jae-hyung,Han, Seong-jun,Lee, Eom-jee,Lee, Jung-eun,Nam, Seong-uk,Ha, Na-yeon,Kim, Jin-sung 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.5

Hypoglossal nerve palsy is an uncommon neurologic disorder. We report a 67-year-old Korean male with tongue paralysis due to hypoglossal nerve palsy. He had complaints associated only with tongue paralysis and was treated with herbal medicine and electric acupuncture for 12 days. We evaluated his tongue paralysis severity by a numeric rating scale (NRS) and the angle of tongue deviation. After 12 days of treatment, the NRS score showed improvement of his complaints, and the angle of tongue deviation was decreased. Korean medicine could therefore be an effective treatment choice for hypoglossal nerve palsy.

총톤수 130톤급 기선저인망 어선 건조해설

이재형,Lee, Jae-Hyeong 선박안전기술공단 1992 漁船 Vol.50 No.-

옥정호와 벼고을(동진강들)

이재형,Lee, Jae-Hyeong 한국하천협회 2006 하천과 문화 Vol.2 No.4

Alginate Lyase 생산 균주 Pseudomonas sp. N7151-6의 분리 및 특성

이재형,배민지,김양춘,남수완,Lee, Jae-Hyung,Bae, Min-Ji,Kim, Yang-Choon,Nam, Soo-Wan 한국미생물·생명공학회 2009 한국미생물·생명공학회지 Vol.25 No.4

해운대 연안에서 그람 음성균이면서 알긴산 분해효소를 생산하는 세균을 분리하였다. 분리된 N7151-6 균주의 성장을 위한 최적 온도는 $30^{\circ}C$, 최적 pH는 8.0으로 조사되었다. 또한 0-7%(w/v) NaCl 농도에서도 성장 가능하다. 16S rDNA 염기 서열 분석과 생화학적 분석에 의해 이 균주는 Pseudomonas 속으로 동정되어 Pseudomonas sp. N7151-6으로 명명하였다. Pseudomonas sp. N7151-6에서 생산하는 알긴산 분해효소를 한외여과(ultrafilteration; MWCO=30 kDa) 방법에 의해 부분정제하였다. 분리된 효소의 최적 pH는 7.0으로 최적 온도는 $30^{\circ}C$로 조사 되었다. pH 5.0에서 9.0까지 이 효소는 안정하였으며, $23^{\circ}C$에서 $37^{\circ}C$까지의 범위에서도 안정성을 보여주었다. 알긴산 분해효소의 전체 활성은 110 unit/L이었다. A Gram-negative, alginate lyase-producing bacterium was isolated from the Haeundae Coast, Korea. The isolated strain N7151-6 produced alginate lyase. The optimal temperature and pH for growth were found to be $30^{\circ}C$ and pH 8.0, respectively. This strain can be grown at the NaCl concentration of 0-7% (w/v). Analysis of 16S rDNA sequence and physiological profiling indicated that the strain N7151-6 belonged to Pseudomonas sp. The enzyme alginate lyase produced by Pseudomonas sp. N7151-6 was partially purified by ultrafiltration (MWCO= 30 kDa). The optimum pH and temperature for the activity of the purified enzyme were found to be 7.0 and $30^{\circ}C$, respectively. The enzyme was stable at the pH range of 5.0-9.0 and temperature range of $23-30^{\circ}C$. The total activity of alginate lyase produced was reached about 110 unit/L.

간헐성 외사시에서 수술시기에 따른 수술결과 비교

이재형,강남여,Jae Hyung Lee,MD,Nam Yeo Kang,MD 대한안과학회 2013 대한안과학회지 Vol.54 No.2

Purpose: To investigate how the constancy of intermittent exotropia affects postoperative motor and sensory outcomes. Methods: A retrospective analysis was conducted of 44 patients with intermittent exotropia (IXT group) and 16 patients with constant exotropia with a previous history of intermittent exotropia (CXT group). All patients were followed up for more than 1 year postoperatively. Successful postoperative motor alignment was defined as within 8 prism diopters of exo- or esotropia at 6 meters and 33 centimeters. Successful sensory outcome (bifixation) was considered as stereoacuity ≤60 seconds of arc. The influence of constancy, age at surgery, duration of strabismus, preoperative gross stereopsis, preoperative distant deviation, and preoperative distant fusion to surgical outcomes were evaluated using logistic regression analysis. Results: Mean age at surgery and follow-up periods was 9.34 years and 37.8 months in the IXT group, and 10.19 years and 45.7 months in the CXT group. Successful motor alignment rates of the IXT and CXT groups were 65.9% and 48.3%, respectively. Postoperative bifixation rates of the IXT and CXT groups were 70.5% and 12.5%, respectively. The IXT group showed significantly better successful sensory outcome (p = 0.000), but no significant difference in motor outcome (p = 0.121). Constancy and preoperative distant fusion were associated with post-operative bifixation in univariate regression analysis (p = 0.001, 0.025, respectively). However, only the constancy was strongly associated with successful sensory outcome in multivariate regression analysis with an odds ratio of 20.138 (p = 0.008). Conclusions: Surgical correction of intermittent exotropia during the intermittent period showed better sensory outcome than in constant deviation period.

무릎 뼈관절염의 단파치료 효과 : 무작위임상시험의 메타분석과 체계적 고찰

이재형,조혁신,송인영,Lee, Jae-Hyoung,Cho, Hyuk-Shin,Song, In-Yong 대한물리치료학회 2014 대한물리치료학회지 Vol.26 No.5

Purpose: The purpose of this study is to assess the effectiveness of short-wave therapy (SWT) for treatment of knee osteoarthritis (OA) as compared to placebo and control, and to assess the question of whether the effects are related to the mode, dosage, and application method. Methods: We searched randomized, placebo-controlled trials using electronic databases. We also manually reviewed sources in order to identify additional relevant studies. Results: Eight studies (597 participants) with OA were included in the meta-analysis. Pulse SWT had a significant effect on pain relief compared with control treatment, while did not favour compared with the placebo group. Pulse SWT had a significant effect on functional improvement compared with control and placebo treatment. Continuous SWT had no effect on pain relief and functional improvement. Capacitive SWT a significant effect on pain relief, functional improvement, and muscle strength. Continuous and capacitive SWT had increased muscle strength significantly. We found no clinical significance of all outcomes except pain and functional improvement in pulsed SWT with low dose. There was no difference in adverse events. None of the participants experienced any serious adverse events. Conclusion: Low dose pulsed SWT provided a short-term clinical benefit for pain relief and functional improvement. Pulsed SWD with low and high dose had effects on pain and function. There seems to be a placebo effect. We found significant effects on pain and function in capacitive SWT. Despite some positive findings, this analysis lacked data on how effectiveness is affected by mode, dosage, and application method of SWT. Further well-designed clinical studies are required in order to confirm the effectiveness of SWT.

홍수시 저수지운영을 위한 시우량 모형 - Hyetograph model for Reservoir operation during Flash flood

이재형,선우중호,정동국,Lee, Jae-Hyeong,Jeong, Dong-Guk 한국수자원학회 1990 물과 미래(한국수자원학회지) Vol.23 No.3

정확한 유출수문곡선의 예보는 강우강도의 예측능력에 좌우된다. 1시간 정도의 단기 강우예측을 위한 추계학적인 강우모형을 개발하여 제시하는 것이 연구과제이다. 개발하고자 하는 모형은 다지점에서 동시에 강우강도를 예상 또는 예측할 수 있는 능력이 있다. 모형에 필요한 매개변수는 TM자료를 비롯하여 과거에 축적된 자료들로 부터 평가된 값을 이용한다. 모형은 강우진행속도, 환상스팩트럼, 무차원 시간분포 등이 선행연구 결과를 토대로 한다. 선택한 무차원 시간분포가 예측에 미치는 영향과 예측모형이 유출수문곡선에 미치는 영향을 분석한다. Precise run-off forecasting depends on the ability to predict quantitative rainfall intensity. This study suggests a stochastic model for 1 hour order rainfall prediction. The model simultaneously predicts rainfall intensity at all telemetered rain-gauge locations. All model parameters, velocity and direction of storm movement, radial spectrum, dimensionless time distribution of rainfall, are estimated from telemetered and historical data for the basin being predicted. Also the estimated parameters are based on the previous study. The results are the influence of dimensionless time distributions on the prediction and the model on run-off.

QSCC II 반복측정결과 동일체질로 판정된 사람의 기질 및 성격분석

이재형,채한,박수진,권영규,Lee, Jae-Hyung,Chae, Han,Park, Soo-Jin,Kwon, Young-Kyu 대한동의생리학회·한의병리학회 2007 동의생리병리학회지 Vol.21 No.5

Incremental DP에 의한 홍수시 댐군의 연계운영

이재형,이길성,정동국,Lee, Jae-Hyeong,Lee, Gil-Seong,Jeong, Dong-Guk 한국수자원학회 1992 물과 미래(한국수자원학회지) Vol.25 No.2

북한강 수계에 위치한 화천댐과 소양강댐을 홍수시 연계운영을 위하여 Incremental DP를 사용하여 최적운영모형을 개발하였다. 목적함수로는 의암댐 합류점의 첨두 유량을 최소화하는 것으로 하였으며 수리-수문학적 제약조건은 홍수시 댐조작에 관한 법령, 각 댐 및 하도특성을 고려하여 설정하였다. 특히, 2차 홍수에 대비하여 홍수가 끝나는 시점의 수위가 제한수위가 되도록 하였다. 또한 Incremental DP 모형의 운영결과와 Simulation 및 DP에 의한 단일댐 운영결과를 조절률과 이용률의 관점에서 비교하였고, 합류점의 첨두유량을 Feedback Loop를 이용한 Simulation 결과와 비교하였다. 운영결과 조절률과 이용률이 Simulation 및 DP에 의한 단일댐 운영결과보다 다소 낮게 나타났고, 합류점 유량이 Feedback Loop에 결과보다 크게 나타났는데 이는 홍수말기의 수위에 관한 제약조건 때문이다. An optimal operation model for flood control of multi-reservoirs, Hwacheon and Soyanggang, located in the north Han River basin is developed by using the Incremental DP. The objective function is to minimize the peak flow at the confluence point, of Euam dam, and the hydraulic and hydrologic constraints are established by considering the related laws as to the operation of dam in flood season, each reservoir and channel characteristics. In particular, the final elevations of each reservoir are induced to the conservation pool level in order to prepare for the secondary flood. In addition, the results of this model, simulation results and the single reservoir operation by DP are compared in terms of control and utility efficiencies, and also the peak flows at the confluence point for floods with various return periods are compared with the results of simulation suing feedback control. as the results, the control and utility effciencies are more or less low in contrast with the results of simulation and the single reservoir operation by DP, and the peak flows at confluence point are high because of terminal condition of reservoir storage.