Phenytoin의 태아 구개열 유발과 모체 혈청 Corticosterone 증가에 대한 Dimethyl Sulfoxide 억제 작용

이재권,이창업,이문한,류판동,이영재,성하정,Lee, Jae-Kwon,Lee, Chang-Eop,Lee, Mun-Han,Ryu, Pan-Dong,Lee, Young-Jae,Sung, Ha-Jung 한국독성학회 1992 Toxicological Research Vol.8 No.2

It is well known that phenytoin (PHT), a commonly prescribed anticonvulsant, has teratogenicity in experimental animals and human. The major malformation induced by PHT in mouse is cleft palate. The mechanisms of the embryotoxic effects of PHT are unknown. However, PHT and synthetic glucocorticoids share several features with respect to their teratogenicity, and it was known that PHT increased maternal corticosterone level. Therefore PHT-induced cleft palate may be mediated indirectly by elevated maternal corticosterone. Recently it was reported that secalonic acid Dinduced cleft palate and elevated endogenous corticosterone level, and that such effects were antagonized by DMSO. The purpose of this work was to investigate whether the elevated maternal corticosterone is associated with the teratogenicity of PHT in the ICR mouse fetuses by treatment with PHT or PHT plus DMSO. PHT (74mg/kg, BW) was daily administered intraperitoneally on day 10~12 of gestation with and without DMSO(2ml/kg, BW), and the fetal malformation was observed on day 18. Maternal serum corticosterone and fetal PHT levels were determined by HPLC. The results are summarized as follows. 1)The percentage of cleft palate incidense in fetuses following treatment with PHT on day 10~12 of gestation was 51.7%. 2)There was a significant decrement in the cleft palate incidence in fetuses to 30.8% in the group treated with PHT plus DMSO compared with 51.7% in that with PHT alone. 3) Maternal serum corticosterone levels following treatment with PHT on day 10~12 of gestation increased by 116~343% compared with that of vehicle control. Such effect was antagonized by DMSO. 4)PHT concentration in the fetuses was not affected by DMSO. These results suggest that PHT-induced cleft-palate in fetuses seems to be closely associated with the elevation of maternal corticosterone level.

ICR마우스에서 phenytoin의 최기형성 및 dimethyl sulfoxide의 항최기형 효과

이재권,이창업,이문한,류판동,조명행,성하정,박진봉,Lee, Jae-kwon,Lee, Chang-eop,Lee, Mun-han,Ryu, Pan-dong,Cho, Myung-haing,Sung, Ha-jung,Park, Jin-bong 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

Phenytoin(PHT), a commonly prescribed anticonvulsant, has been known as a teratogen in experimental animals and human. However, PHT has strain-specific teratogenic effects for mice and human. Dimethyl sulfoxids(DMSO) has been known to antagonize the teratogenic effects of secalonic acid D, a toxic mold metabolite that has similar teratogenic effects to PHT. Therefore, this study was performed to examine the embryopathic effects of PHT in terms of treatment period and the antiteratogenic effect of DMSO in ICR mice. PHT(75mg/kg, BW) was administered intrapetitoneally on day 10, 10-11 and 10-12 of gestation with or without DMSO(2ml/kg, BW), and the fetal malformation was observed on day 18. Major malformation of fetuses treated with PHT on day 10, 10-11 and 10-12 of gestation was cleft palate, and the percentages of fetus with cleft palate were 14.5%, 31.7% and 51.7%, respectively. Also, there was a significant decrease of cleft palate from 51.7% in PHT alone group to 30.8% in PHT plus DMSO group. Our findings suggest that cleft palate is one of major malformation by PHT treatment in ICR mouse and DMSO has strong antiteratogenic effect.

Interleukin-8-like chemotactic factor from feline peripheral blood mononuclear cells cultured with egg white derivatives

이재권,양만표,Lee, Jae-kwon,Yang, Mhan-pyo The Korean Society of Veterinary Science 2000 大韓獸醫學會誌 Vol.40 No.2

계난백유래물질(EWD)로 배양한 고양이 말초혈액 단핵구세포(MNC)의 배양상충액에서 다형핵백혈구(PMN)에 대한 유주성인자를 조사하였다. EWD로 배양한 MNC 배양상층액과 human recombinant (hr) interleukin (IL-8)는 고양이 PMN의 유주성을 현저하게 증가시켰다. 이 유주활성물질을 규명하기 위해 EWD 처리 MNC 배양상충액을 gel electro-phoresis(denaturing 조건 18% loading gel 및 nondenaturing 조건 12.5% loading gel)를 실시한 결과, EWD로 배양한 MNC 배양상충액과 hr IL-8는 모두 분자량 6~8kDa에서 band를 나타내었다. Denaturing 조건에서 6~8kDa band의 gel slices에서 용출시킨 용출액에서도 고양이 PMN의 유주활성이 인정되었다. EWD로 배양한 MNC 배양상층액, hr IL-8 및 용출액에 의한 유도된 고양이 PMN의 유주활성은 rabbit anti-feline polyclonal IgG(RAF pIgG)와 hr IL-8에 대한 mAb에 의해 농도의존적으로 억제되었다. 또한 RAF pIgG는 hr IL-8와 결합을 보임으로써 사람의 IL-8와 교차반응을 시사하였다. 이상의 결과로부터 EWD로 배양한 고양이 MNC는 분자량 6~8kDa의 IL-8 양(樣) 유주성인자를 분비하여 PMN의 유주성을 유도하는 것으로 사료되었다. The feline chemotactic factor(s) for polymorphonuclear cells (PMN) in culture supernatant from mononuclear cells (MNC) treated with egg white derivatives (EWD) were examined. Culture supernatant from MNC treated with EWD and human recombinant (hr) IL-8 remarkably enhanced chemo-taxis of feline PMN. To investigate feline chemotactic factor(s), gel electrophoresis was performed with culture supernatant from MNC treated with EWD under denaturing (18% loading gel/5% stacking gel) and nondenaturing (12.5% loading gel/5% stacking gel) condition. Hr IL-8 and culture supernatant from MNC treated with EWD yielded a distinct band in a molecular weight, 6 to 8 kDa. Eluted solution from gel slices of 6 to 8 kDa band in denaturing condition also enhanced feline PMN chemotaxis. These chemotactic activities of feline PMN induced by culture supernatant from MNC treated with EWD, hr IL-8 and eluted solution were inhibited in a dose-dependent manner by rabbit anti-feline polyclonal IgG (RAF pIgG) and monoclonal antibody (mAb) against hr IL-8. RAF pIgG also showed a binding activity with hr IL-8, suggesting that RAF pIgG against feline IL-8-like chemotactic factor(s) had cross-reactivity with human IL-8. These results suggested that feline MNC treated with EWD might release feline IL-8-like chemotactic factor(s) with a molecular weight, 6 to 8 kDa, which induces the chemotaxis of feline PMN.

인후두역류질환의 진단 및 치료 경향; 이비인후과 전문의 설문조사 결과

이재권,김태욱,손영익,Lee, Jae-Kwon,Kim, Tae-Wook,Son, Young-Ik 대한후두음성언어의학회 2009 대한후두음성언어의학회지 Vol.20 No.2

Background and Objectives: Although laryngopharyngeal reflux disease (LPRD) is a widely recognized disease in the field of otolaryngology, controversies still exist regarding the diagnosis and treatment for it. The purpose of this survey was to find out current trends in diagnosis and management of LPRD among Korean otolaryngologists. Materials and Method: Questionnaires were obtained from 26 Korean otolaryngology specialists by electronic balloting, and 46 members of the Korean Society of Head and Neck Surgery (KSHNS) bye-mail. Survey participants were asked about their preferences in diagnostic policy, duration/dosage of antireflux treatment, and their familiarity to the reflux symptom index (RST) and the reflux finding scores (RFS). Results: The majority of the survey participants (64%) preferred empiric therapy trial with proton pump inhibitors (PPIs) based on patients' symptoms and their laryngeal findings. PPIs alone (27%) or PPIs with prokinetics (32%) were usually prescribed as a first line treatment mostly with once daily schedule in the morning before meal and for less than 4 months of duration (86%). More than 90% of participants were aware of the RSI and RFS, but less than 50% of them were actually using those items in the clinics. Conclusion: Most of Korean otolaryngologists who participated in this survey answered that they prefer starting empirical treatments with proton pump inhibitors mainly based on the patient's symptoms and their laryngeal findings.

평창동계올림픽 경기장 방문동기가 만족도 및 행동의도에 미치는 영향

이재권(Lee, Jae-Kwon),임세준(Lim, Sei-Jun) 한국체육과학회 2018 한국체육과학회지 Vol.27 No.3

The purpose of this study was to analyze visiting motivation, satisfaction and visiting experience at a 2018 Pyeongchang Winter Olympic Games venue and assess how this will affect behavioral intentions of visitors to a venue of the host city. It focused especially on the Alpensia Ski Jumping Center and aims to analyze influencing factors among visiting motivation, satisfaction and behavioral intentions and provide key data for promotion, services and legacy planning. To achieve the objective of the study, the sampling target was 392 visitors to the Alpensia Ski Jumping Center which is one of the 2018 Winter Olympic Games venues. Convenience sampling and self-administration methods were used as research methods. This study used SPSS Statistics 18 for analysis of frequency, exploratory factor, reliability, correlation analysis, simple regression and multiple regression analysis. After verifying the goodness of the fit of the model and verifying the hypothesis, this study reached three conclusions. Firstly, motivations of visiting the venue were factored into five factors. Among the factors of visiting motivation, Olympic attraction, novelty, socialization, and escape have influence on the overall satisfaction. Secondly, visiting satisfaction has an influence on future behavioral intentions (revisit and recommendation intention). Finally, among the factors of visiting motivation; Olympic attraction, socialization, and escape have an influence on future behavioral intentions (revisit and recommendation intention). Novelty and family only have an influence on the recommendation intention.

다형의 버그 추적 시스템 마이닝 및 분석을 위한 저장소 독립 모델 설계

이재권(Jae-Kwon Lee),정우성(Woo-Sung Jung) 한국컴퓨터정보학회 2014 韓國컴퓨터情報學會論文誌 Vol.19 No.9

본 논문은 다양한 버그 추적 시스템으로부터 추출한 데이터를 통합하여 단일 저장소 모델을 제공하는 UniBAS(Unified Bug Analysis System)를 제안한다. UniBAS는 MSR(Mining Software Repositories) 연구 과정에서의 저장소 추출, 데이터 가공이나 모델 생성과 같은 공통적인 반복 작업을 줄이고, 관련 연구자가 상위 수준의 연구에 보다 집중할 수 있도록 함으로써 해당 연구 수행에 발생하는 복잡도와 비용을 줄여준다. 또한, UniBAS는 데이터 추출 뿐 아니라 질의 기반 분석에 필요한 테이블, 뷰 및 저장 프로시저 등을 자동 생성하며, 수집한 데이터 관리와 외부 도구와의 연동을 위해 다양한 형식의 파일을 생성할 수 있다. 사례 연구로 UniBAS의 유용성을 검증하기 위해 Mozilla사이트의 Firefox프로젝트를 대상으로 실제 중복 버그 리포트를 탐지하는 실험을 진행하였다. 이 과정에서 자동 추출된 자료를 대상으로 질의와 분석이 유연하게 이루어질 수 있었으며, 다양한 자연어 처리 알고리즘 적용을 통해 유효한 실험 결과를 얻을 수 있었다. In this paper, we propose UniBAS(Unified Bug Analysis System) to provide a unified repository model by integrating the extracted data from the heterogeneous bug tracking systems. The UniBAS reduces the cost and complexity of the MSR(Mining Software Repositories) research process and enables the researchers to focus on their logics rather than the tedious and repeated works such as extracting repositories, processing data and building analysis models. Additionally, the system not only extracts the data but also automatically generates database tables, views and stored procedures which are required for the researchers to perform query-based analysis easily. It can also generate various types of exported files for utilizing external analysis tools or managing research data. A case study of detecting duplicate bug reports from the Firfox project of the Mozilla site has been performed based on the UniBAS in order to evaluate the usefulness of the system. The results of the experiments with various algorithms of natural language processing and flexible querying to the automatically extracted data also showed the effectiveness of the proposed system.

배위의 현학사상

이재권 ( Lee Jae Kwon ) 한국사회과교육학회 1987 시민교육연구 Vol.11 No.1

IPA 기법을 이용한 볼링장 선택 속성의 중요도와 만족도 분석

이재권(Lee, Jae-Kwon),오경록(Oh, Kyung-Rok),김동규(Kim, Dong-Kyu) 한국체육과학회 2018 한국체육과학회지 Vol.27 No.4

The purpose of this study was to provide effective information on the marketing strategy through IPA analysis of the attributes of bowling arena. toward this end, a total of 326 respondents was selected using a convenient sampling method and responded to the survey questionnaire. 326 usable data were utilized in data analysis procedure. data were analyzed using, exploratory factors analysis, reliability analysis, descriptive statistics analysis and IPA analysis. The result of this study were as follows. Firstly. 1 quadrant included employee kindness, lane condition, internal environment, location convenience, parking convenience. Secondly. 2 quadrant included fee, sale promotion. Thirdly. 3 quadrant included food & beverage service, reserve system, event, ball condition, food & beverage cost, public transportation convenience. Fourthly. 4 quadrant included rest area, rain scale. discussions and additional implications were also suggested.

인체 부위의 감각 차이 탐구 (과학영재교육을 위한 자료)

이재권 ( Jae Kwon Lee ) 충북대학교 과학교육연구소 2016 과학교육연구논총 Vol.32 No.1

A sense is a physiological ability of living things that provides information for consciousness. Human senses are traditionally clssified as sight(ophthalmoception), hearing(audioception), taste(gustaoception), smell(olfacoception), and touch(tactioception). The senses allow human to learn, to protect, to enjoy fun things. Sensor organs operate by coordinative activity of nerve system, cognitive psychology, and philosophy of perception. The nervous system has a specific sensory system or organ, dedicated to each sense. In this study 1 introduce new program to educate the sensor organs for the students of the course of gifted and talented education. This program will be contribute to understand of touch sense.

멀미약의 약리 활성을 위한 교육자료

이재권 ( Jae Kwon Lee ) 충북대학교 과학교육연구소 2015 과학교육연구논총 Vol.31 No.1

Motion sickness is the feeling you get when the motion you sense with your inner ear is different from the motion you visualize. It is a common condition that occurs in some people who travel by car, train, airplane or boat. Motion sickness progresses from a feeling of uneasiness to sweating and dizziness. This is usually quickly followed by nausea and vomiting. Many motion sickness medication which are effective for motion sickness. However, most motion sickness medications carry the possibility of side effects. Therefore in this study we demonstrated the pharmacokinetic effect of motion sickness medications of cardiovascular system using a water flea.