Immunogold법에 의한 한국재래산양 샘뇌하수체의 성샘자극세포에 관한 연구

이인세,이흥식,송승훈,윤성태,황인구,서제훈,강태천,원무호,Lee, In-se,Lee, Heungshik S.,Song, Seung-hoon,Yoon, Sung-tae,Hwang, In-koo,Seo, Je-hoon,Kang, Tae-cheon,Won, Moo-ho 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.5

There have been a number of studies of gonadotropes secreting LH and FSH in the adenohypophysis, but the pattern of hormone storage and secretion of these cells still remains a controversial matter. In this study, we examined whether gonadotropes contained both of LH and FSH, and if so, how these hormones were distributed within the secretory granules. Hypophyseal sections of Korean native goat were simultaneously immunostained for LH and FSH antisera by protein A-gold technique. It was found that most gonadotropes contained both FSH and LH, but hormone storages in the secretory granules were some different among cells. Three types of gonadotropes were identified by the shape and size of the secretory granules and their hormone storage patterns. One type(I) of gonadotropes contained oval secretory granules, which immunoreactivity for FSH and LH were very weak. The size of secretory granules ranged from 160 to 310nm in diameter. Most granules contained both FSH and LH, but some contained only one of them. In another type(II) of gonadotropes, the immunreactivity and hormone storage patterns of the secretory granules were similar to those of type I cells. However, the secretory granules were round in shape and larger in size than those of type I. The other gonadotropes(type III) were distinctly distinguished by plenty of hormones in their secretory granules which were densely packed with numerous immunolabelled gold particles. These data are some inconsistent with other results that have been obtained in other ruminants like as cattle and sheep.

제초제 저항성 논잡초 발생동향

이인용(Lee In-Yong),박중수(Jung-Soo Park),서영호(Young-Ho Seo),김은정(Eun-Jung Kim),이순계(Sun-Gye Lee),조승현(Seng-Hyun Cho),권오도(Oh-Do Kwon),김상국(Sang-Kuk Kim),정완규(Wan-Gyu Chung),박태선(Tae-Seon Park),김창석(Chang-Seok Kim) 韓國雜草學會 2012 Weed&Turfgrass Science Vol.32 No.2

제초제 저항성 논잡초 발생면적은 농촌진흥청 국립농업과학원과 전국 8개 도 농업기술원과 공동으로 조사하였다. 8개 도 농업기술원에서 2011년 8월에 논토양을 채취하여 피라조설퓨론에틸 피리미노박메틸입제를 30kg ha-1 처리하여 설포닐우레아(SU)계 제초제 저항성 논잡초의 발생면적을 추산하였다. 전국 벼 재배면적(유기 및 친환경농업 벼 재배면적 제외)의 20.9%인 167,081ha에서 SU계 저항성 논잡초가 발생되고 있음을 확인하였다. 2008년과 대비하여 본 결과 60,130ha가 늘어났다. 발생비율을 보면, 충청남도가 논 면적의 47.6%로 제일 많이 발생되었고, 그 다음으로는 전라남도로 36.9%, 충청북도 25.7%, 강원도 20.3%, 경기도 13.0% 순이었다. 제초제 저항성 논잡초 중 발생면적이 가장 넓은 초종은 물달개비로서 65,313ha로 전체 39.1%를 차지하였으며, 그 다음으로 올챙이고랭이, 알방동사니 순이었다. 이들 3초종은 전국적으로 고르게 발생하여 제일 문제되는 잡초들이다. 그리고 미국외풀 13,964ha(8.4%), 강피 5.1%이었다. National Academy of Agricultural Science and eight province Agricultural Research & Extension Services investigated the occurring area of herbicide resistant weeds in paddy field of Korea. In order to estimate the occurring areas of herbicide resistant weeds, we collected paddy soils on August, 2011 and treated 30kg $ha^{-1}$ of pyrazosulfuron-ethyl+pyriminobac-methyl GR. 167,081ha, approximately 20.9% of cultivated area excluding organic and eco-friendly cultivated area, was estimated to be infested by SU-herbicide resistant paddy field weeds. It was increased by 60,130ha compared with investigation of 2008. It was occurred at Chungchungnam-do by 47.6%, followed by 36.9% at Jeonranam-do, 25.7% at Chungchungbuk-do, 20.5% at Gangwon do, and 13.0% at Gyeonggi-do, respectively. Monochoria vaginalis showed the highest with 65,313ha, 39.1% followed by Scirpus juncoides, and Cyperus difformis, respectively. These three species were evenly distributed and the most problematic weeds in the country. Lindernia dubia occurred at 13,964ha (8.4%) and Echinochloa oryzicola was 5.1%, respectively.

다층성장모형을 활용한 산학협력 유형별 대학의 영향요인 분석: 제도주의 동형화와 자원의존이론 관점에서

이인서(Lee, In-Seo) 한국교육정치학회 2020 敎育政治學硏究 Vol.27 No.4

최근 고등교육기관은 산업과 연계하여 산업에 필요한 인재를 육성하거나 기술을 개발하는 등 국가산업의 발전에서 주요한 역할을 하고 있다. 우리나라에서는 정부가 대학의 특성에 맞는 산학협력을 장려하는 재정지원사업을 실시하여 고등교육기관에 산학협력 기능이 정착되었다는 특징이 있다. 본 연구는 국내 고등교육기관에 산학협력이 확산된 방식을 제도주의 동형화의 관점과 자원의존이론 관점에서 분석해보고, 대학의 특성에 맞는 산학협력 방식이 정착되어있는지 분석하고자 하였다. 분석을 위해 2018~2020 대학정보공시자료를 활용하였고, 다층성장모형(multilevel growth model) 방법을 사용하여 전국 209개 대학의 유형별로 인력양성중심 산학협력과 기술개발중심 산학협력 중 어떤 유형이 이루어지고 있는지 분석하였다. 분석 결과 개별 대학에서 산학협력 기능을 수용하는 과정에서 대학의 특성(대학 사명, 지역)에 부합하는 산학협력이 선택되는 것으로 나타났다. 연구 결과에 근거하여 국내의 산학협력 활성화를 위한 시사점을 제시하였다. Recently, institutions of higher education have played a major role in the development of national industries, such as fostering human resources needed for industry or developing technology in connection with industry. This study analyzed the diffusion of industry-academic cooperation in domestic higher education institutions from the perspective of institutionalization and the theory of resource dependence, and analyzed whether industrial-academic cooperation methods were established to suit the characteristics of universities. For analysis, the 2018-2020 university information disclosure data was used, and the multilevel growth model method was used to analyze the types of industry-academic cooperation centered on human resources development and research and technology transfer. The analysis showed that individual universities chose industry-academic cooperation that fits to the university s characteristics (college mission, region) in the process of accepting industry-academic cooperation functions. Based on the research results, this study presented implications for establishing industry-academic cooperation functions.

한국재래산양 뇌하수체의 성장자극세포와 젖샘자극세포에 관한 전자현미경적 연구

이인세,이흥식,원무호,서제훈,송승훈,남영삼,강태천,Lee, In-se,Lee, Heungshik S.,Won, Moo-ho,Seo, Jehoon,Song, Seung-hoon,Nam, Young-Sam,Kang, Tae-Cheon 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3

Somatotropes, mammotropes and somatomammotropes of the Korean native goat hypophysis were studied by double immunoelectron microscopy using antisera to growth hormone(GH) and prolactin(PRL), and protein A-gold particles of different sizes. Mammotropes were round or oval in shape, and contained round and electron dense secretory granules. The size of secretory granules was variable from 460nm to 680nm in diameter. Somatotropes were elliptical or triangular in shape and the oval nucei were located eccentrically at the periphery of the cell. Secretory granules of the cell were oval in shape and clearly distinguished from round granules of mammotropes. The size of granules was 320~680nm in diameter, smaller than that of mammotropes. Somatomammotropes contained round or oval secretory granules. The granules had intermediate size between somatotropes and mammotropes. Some of granules contained both GH and PRL, while the others contained only one of them.

한국재래산양 뇌하수체의 성장자극세포 및 젖샘자극세포에 관한 면역조직화학적 연구

이인세,이흥식,강태천,서제훈,송승훈,남영삼,Lee, In-se,Lee, Heungshik S.,Kang, Tae-Cheon,Seo, Jehoon,Song, Seung-hoon,Nam, Young-Sam 대한수의학회 1998 大韓獸醫學會誌 Vol.38 No.3

The localizations and morphological characteristics of immunoreactive cells for prolactin(PRL) and growth hormone(GH) antisera were studied with double immunohistochemistry in the adenohypophysis of the Korean native goat. PRL immunoreactive cells(mammotropes) and GH immunoreactive cells(somatotropes) were present in the pars distalis and pars tuberalis, but not in the pars intermedia. A few cells were stained with both PRL and GH antisera (somatomammotropes). The possessional percentages of mammotropes, somatotropes and somatomammotropes were 37.0%, 32.6%, 1.0% in females and 35.6%, 32.6%, 1.1% in males, respectively. Mammotropes were oval or round in shape, and $10{\sim}20{\mu}m{\times}17{\sim}25{\mu}m$ in size. These cells were distributed throughout the pars distalis, but were more abundant on the dorsal part adjacent to the hypophyseal cavity and along the lateral and ventral peripheral regions. Somatotropes were elliptical, triangular or polygonal in shape, and $8{\sim}17{\mu}m{\times}17{\sim}18{\mu}m$ in size. The distribution pattern of somatotropes was similar to that of mammotropes. Some somatomammotropes were intercalated between clusters of mammotropes while the others were dispersed independently among the secretory cells.

한국재래산양 십이지장의 장관신경계통에 분포하는 Substance P, CGRP 및 칼슘결합단백질 반응세포에 대한 면역조직화학적 연구

이인세,이흥식,송승훈,윤성태,황인구,강태천,원무호,서제훈,Lee, In-se,Lee, Heungshik S.,Song, Seung-hoon,Yoon, Sung-tae,Hwang, In-koo,Kang, Tae-cheon,Won, Moo-ho,Seo, Je-hoon 대한수의학회 1999 大韓獸醫學會誌 Vol.39 No.3

The localization of substance P(SP), calcitonin gene-related peptide(CGRP) and three calcium binding proteins, calbindin D-28k(CB), calretinin(CR) and parvalbumin(PA) was immunohistochemically examined in the myenteric and submucous plexuses of Korean native goat duodenum. In the neurons of myenteric and submucous plexuses of duodenum, immunoreactivities of SP, CGRP and CB were confirmed in both nerve cell bodies and fibers. In contrast, CR immunoreactivity was found only in nerve fibers of myenteric plexuses, while PA immunoreactivity was found only in nerve cell bodies of submucous plexuses. In the inner circular muscle layer, dense SP-like immunoreactive fibers were prominent but only a few CGRP-like immunoreactivities were observed. Most of SP- and CGRP-like immunoreactive neurons of both plexuses colocalized with CB. This result showed that SP and CGRP may have a important role for the movement of small intestine. The colocalizations of CB with SP or CGRP in myenteric and submucous plexuses suggest that CB may serve neuromodulatory role for SP- and CGRP-immunoreacted neurons on the movement of intestinal wall.

전산유체역학 기법을 이용한 돈사 내 습식 공기 정화기의 적정 위치 설계

권경석,이인복,황현섭,홍세운,서일환,최지선,송상현,문운경,Kwon, Kyeong-Seok,Lee, In-Bok,Hwang, Hyun-Seob,Bitog, Jessie.P.,Hong, Se-Woon,Seo, Il-Hwan,Choi, Ji-Sun,Song, Sang-Hyeon,Moon, Oun-Kyung 한국농공학회 2010 한국농공학회논문집 Vol.52 No.3

In South Korea, as the living standard has been getting higher, meat consumption is steadily increasing. To meet the country's demand, livestock houses become larger and wider with increased raising density. In larger livestock houses, pollutants such as flake of pig skin, excrement, odor, various dusts and noxious gas like ammonia are excessively accumulated inside the facility. These will cause weak immunity for the pigs, diminution of productivity and degeneration of working condition. These problems can be solved through the ventilation performance of the facility. In the winter time, ventilation must be controlled to minimum to maintain a suitable thermal condition. However, this affects the other internal environmental condition because of the minimum ventilation. The installation of "wet air cleaner" especially in the winter time can be an alternative solution. For efficient application of this machine, there is a need to understand the existing ventilation condition and analyze the interaction of existing ventilation system with the wet air cleaner considering its appropriate location. In this study, the existing ventilation system as well as the internal environmental condition negatively inside the facility with the wet air cleaner has been studied using CFD technology. The CFD simulation model was validated from the study conducted by Seo et al. (2008). Results show that the elimination rate of ammonia was 39.4 % and stability could be improved to 35.1 % (Comparing case 5 to 1 where wet air cleaner machine was not used). It can therefore be concluded that case 5 shows the optimum location of a wet air cleaner in the livestock house.

절연구간 전원절체 개폐장치 신뢰도 예측

이인현(In-Hyun Lee),하승태(Seung-Tae Ha),김민호(Min-Ho Kim),서정욱(Jung-Wook Seo),김두일(Du-Ill Kim),홍현표(Hyun-Pyo Hong),이병곤(Byung-Gon Lee),반극동(Keuk-Dong Ban) 한국철도학회 2012 한국철도학회 학술발표대회논문집 Vol.2012 No.10

본 논문은 자동 전원절체 통과시스템을 대상으로 수행 중인 신뢰도 및 위험도 분석 과제 결과의 일부분이다. 자동 전원절체 통과시스템은 전차선로 절연구간을 전기차량이 무정전으로 통과할 수 있도록 개발한 시스템으로 크게 열차검지장치와 전원절체 개폐장치로 구성된다. 이 중에서 전원절체 개폐장치를 대상으로 신뢰도 분석을 수행한 것으로 각 컴포넌트의 고장률 예측을 위한 방법, 절차 그리고 시스템 신뢰도 모델링을 수행한 결과를 기술하였다. 신뢰도 예측은 Telcordia SR-332 Issue 2 규격서를 사용하였으며, 향후 이 결과는 위험도(risk) 분석을 위한 기반 자료로 이용될 예정이다. This paper is parts of a result of a project on reliability and risk analysis for automatic changeover system. The automatic changeover system is developed in order that an electrical train can pass through catenary neutral section in an uninterruptible way, and it’s comprised of a train detector and a power switching system. Reliability analysis is performed for a power switching system of them, and a method and a process to predict a failure rate of each component and a result of performing a system reliability modeling are described in this paper. Reliability prediction is in accordance with Telcordia SR-332 Issue 2, and the result will be used for risk analysis as basic input in the future.

축산악취 확산의 정성적 및 정량적 예측을 위한 공기유동학적 모델 개발 (1) 축산악취 확산 정도 조사 및 이의 영향 요인 규명

이인복 ( Lee In Bok ),홍세운 ( Hong Se Woon ),황현섭 ( Hwang Hyun Sup ),서일환 ( Seo Il Hwan ),유재인 ( Ru Jae In ),하태환 ( Ha Tae Hwan ),권경석 ( Kwan Hyung Suk ) 한국농공학회 2007 한국농공학회 학술대회초록집 Vol.2007 No.-

Odor is one of the major nuisances in the environment. Especially, odor annoyance from livestock production is an increasing problem in communities in many countries. In order to reduce the odor inconvenience and establish good relations between livestock industry and surrounding communities, many studies on the odor dispersion, such as field experiment and diffusion simulation, have been investigated. Especially, CFD being used widely will be effective for aerodynamic analysis with consideration for various wind conditions as well as topographical conditions. Therefore the ultimate objective is to develop the aerodynamic model for predicting qualitative and quantitative diffusion of livestock odor, and in this study, phenomena and factors of livestock odor diffusion were investigated previously.

전산유체역학을 이용한 표준 오리사 설계안에 대한 환기효율성 분석

여욱현,조예슬,권경석,하태환,박세준,김락우,이상연,이승노,이인복,서일환,Yeo, Uk-Hyeon,Jo, Ye-Seul,Kwon, Kyeong-Seok,Ha, Tae-Hwan,Park, Se-Jun,Kim, Rack-Woo,Lee, Sang-Yeon,Lee, Seung-No,Lee, In-Bok,Seo, Il-Hwan 한국농공학회 2015 한국농공학회논문집 Vol.57 No.5

In Korea, 69.4 % of duck farms had utilized conventional plastic greenhouses. In this facilities, there are difficulties in controlling indoor environments for raising duck. High rearing density in duct farms also made the environmental control difficult resulting in getting more stressed making their immune system weaker. Therefore, a facility is needed to having structurally enough solidity and high efficiency on the environmental control. So, new design plans of duck house have recently been conducted by National Institute of Animal Science in Korea. As a study in advance to establish standard, computational fluid dynamics (CFD) was used to estimate the aerodynamic problems according to the designs by means of overall and regional ventilation efficiencies quantitatively and qualitatively. Tracer gas decay (TGD) method was used to calculate ventilation rate according to the structural characteristics of duck houses including installation of indoor circulation fan. The results showed that natural ventilation rate was averagely 164 % higher than typically designed ventilation rate, 1 AER ($min^{-1}$). Meanwhile, mechanically ventilated duck houses made 81.2 % of summer ventilation rate requirement. Therefore, it is urgent to develop a new duck house considering more structural safety as well as higher efficiency of environmental control.