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Latex 응집반응을 이용한 동물의 톡소플라즈마병 진단액 개발에 관한 연구
서명득,이응구,Suh, Myung-deuk,Lee, Eung-goo 대한수의학회 1993 大韓獸醫學會誌 Vol.33 No.4
This study was conducted to develop a sensitized latex-antigen for serodiagnosis of toxoplasmosis in animals. Tachyzoites of T gondii(RH-strain) harvested from mouse peritoneal cavity were purified through the filtraton of polycarbonate membrane(pore size, $3.0{{\mu}m}$, Costar Co.) and disrupted by ultrasonicator. The tachyzoite suspension was ultracentrifuged for 30 min at $60,000{\times}g(4{^{\circ}C})$ and the supernatant was used as a water-lysate antigen. Polystyrene latex particles of $0.8{{\mu}m}$ in diameter(Sigma) were used for the preparation of sensitized latex-antigen suspension. The several parameters including the preparation conditions, incubation buffer. serum dilution buffer and stability of agglutination reactions were evaluated and the results obtained were summarized as follows : 1. The antigen consisting of a water-lysate of T gondii tachyzoites was adsorbed onto polystyrene latex particles of $0.8{{\mu}m}$ in diameter by adding a latex suspension to an equal volume of diluted antigen solution and by incubating the mixture at $37{^{\circ}C}$ under different conditions. 2. The optimum incubation buffer used for the antigen sensitization was 0.1M Tris-HCl buffer(pH 8.0). 3. The optimum serum dilution buffer used for the latex agglutination test was 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300 mM NaCl. But 0.1M Tris-HCl-NaCl buffer(pH 7.4) containing 300-600 mM NaCl, 0.5% BSA and 0.01% Tween-20 improved the agglutination pattems and cleared the background of microplate well without the effects on L.A titer. 4. The time required for antigen sensitization was 40 and 60 min in incubation buffer(pH 8.0) at $37{^{\circ}C}$. But the optimun time for antigen sensitization was min at $37{^{\circ}C}$. 5. The optimun quantity of antigen absorbed on latex particles for proper agglutination was the range of 20 to $32{\mu}g$ of latex particles. 6. The optimun concentration of the latex-antigen suspension for the proper agglutination reaction was determined as 0.2%(w/v). 7. The specificity, rapidity and simplicity of the latex-particle agglutination test suggested that it might be adaptable to large scale serum screening.
Latex 응집반응과 간접형광항체법을 이용한 개 톡소플라즈마병의 혈청학적 진단
이병훈,이응구,서명득,Lee, Byung-hoon,Lee, Eung-goo,Suh, Myung-deuk 대한수의학회 1992 大韓獸醫學會誌 Vol.32 No.4
This study was conducted to determine the serum antibodies against toxoplasma in the artificially infected dogs, pet and street dogs by latex agglutination (LA) and indirect fluorescent antibody (IFA) test. LA test was carried out with commercial Toxo-MT kit (Eiken chemical Co.) and IFA test was carried out with rabbit-anti-dog IgG labelled with FITC (Cappel Co.) and toxo-antigen slides prepared in laboratory. The results obtained were as follows ; 1. Antibodies to Toxoplasma gondii in the artificially infected dogs were detected firstly at the Day 8 in IFA and Day 9 in LA test after inoculation. Positive antibody reactions by these tests were declined gradually afterward but maintained up to 12 weeks. 2. In LA test serum antibody titers in 310 test sera were shown as 10 cases(32%) in 1 : 32.5(1.0%) in 1 : 64, 4(1.3%) in 1 : 128 and 2(0.7%) in 1 : 256. In IFA test serum antibody titers 310 test sera were shown as 17 cases(5.5%) in 1 : 64, 8(2.6%) in 1 : 128 and 5(1.6%) in 1 : 256. 3. In the total of 310 sera from pet and street dogs toxoplasma antibody positive rates were 21 cases (6.8%) by LA and 30 cases (9.7%) by IFA test and the positive detection rates between these two groups by LA and IFA test were not significant(p<0.05). 4. In the total of 115 sera from pet dogs toxoplasma antibody positive rates were 12 cases(10.4%) by LA and 15(13.0%) by IFA test. And in the 195 street dogs the positive rates were 9 cases(4.6%) by LA and 15(7.7%) by IFA test. Also, the positive detection rates between these two groups by LA and IFA test were not significant(p<0.05). 5. Agreement of reactivity between LA and IFA test for 310 sera was 91.3% in total of 283 cases consisting of 12 cases(3.9%) of both LA and IFA positive and 271 cases(87.4%) of LA and IFA negative. 6. LA test was almostly equivalent to the IFA test in producibility and proved to be a simple tool for the screening of toxoplasma antibody in laboratory.
DACUM 직무분석을 이용한 산학일체 교육과정개발 -마이크로컴퓨터 제어 직무를 중심으로-
김영오,김병래,이응구,조병섭,Kim, Young-Oo,Kim, Hyeong-Rae,Lee, Eung-Gu,Cho, Byung-Seob 대한전자공학회 2000 電子工學會論文誌 IE (Industry electronics) Vol.37 No.4
직무분석을 도입하여 대학의 교육과정을 구축하는 모델을 제시하고, 실제 적용한 결과를 예시하였다. 산업현장의 요구사항을 충실히 교육과정으로 구축하기 위하여 DACUM을 도입하였으며, 이를 이용하여 교육과정을 설계한 결과 기존의 교육과정에 비해 현장 중심적인 교과목이 신설되거나, 실무적인 내용이 많이 추가됨을 확인하였다. A curriculum-building model is presented to achieve a work based curriculum development. It includes a job analysis method called DACUM(Developing A Curriculum) which is designed to accept various requirements of industrial fields fast and faithfully. The model is applied to design our curriculum. By analysis, it is shown that the new curriculum includes some new practical classes, and/or that new practical contents are included even in old classes.
이차원전기영동을 이용한 Escherichia coli O157:H7 균주간 항원 Spot의 비교
안영창,신기욱,신용승,이응구,이형준,박미림,김영림,정태성,김곤섭,김용환,Ahn, Yeong-Chang,Shin, Gee-Wook,Shin, Yong-Seung,Lee, Eung-Goo,Lee, Hyoung-Jun,Park, Mi-Rim,Kim, Young-Rim,Jung, Tae-Sung,Kim, Gon-Sup,Kim, Yong-Hwan 대한수의학회 2002 大韓獸醫學會誌 Vol.42 No.2
Proteomics is an emerging powerful tool in studying protein expression and function. At present study, proteomics was employed to evaluate the antigenicity among Escherichia coli O157:H7 strains using 2-dimensional gel electrophoresis (2-DE) and immunoblotting, SDS-PAGE and immunblotting analysis revealed no big differences among E coli O157:H7 strains. 2-DE analysis, however, revealed common antigens as well as specific antigens. The immunoblotting analysis revealed 20 common antigenic spots among E coli O157:H7 strains. In addition, there were 3 and 13 spots as common antigens between ATCC 43894 and KSC 109, and between ATCC 43894 and ACH 5, respectively. Antigenic spots specific for individual strain were also identified as 15, 8 and 22 for ATCC 43894, ACH 5 and KSC 109, respectively. The common antigens would be useful by employing either vaccine development or diagnosis marker, or both, whereas the specific antigens of individual strains would be applicable for epidemiological study. This study suggest that proteome analysis, representative as 2-DE, is valuable tool in exploring the E. coli antigenicity.
Proteomics 기법을 이용한 Salmonella enteritidis의 항원 단백질 분석
박미림,신용승,한대용,김용환,정태성,이후장,이응구,김종수,김은희,김곤섭,Park, Mi-rim,Shin, Yong-seung,Han, Dae-yong,Kim, Yong-hwan,Jung, Tae-sung,Lee, Hu-Jang,Lee, Eung-Goo,Kim, Jong-su,Kim, Eun-hee,Kim, Gon-Sup 대한수의학회 2004 大韓獸醫學會誌 Vol.44 No.1
The common pathogen Salmonella enteirtidis (S. enteritidis) is the major cause of foodborne disease. Protein identification by peptide mass fingerprinting using the matrix-assisted laser desorption ionization time of fight (MALDI-TOF) mass spectrometry (MS) can analysis unambiguously identity the spots from 2-dimensional electrophoresis (2-DE) gel. In this report, we examined protein components from patterns of S. enteritidis proteins. In addition, antigens that are recognized by sera can be identified by immunoblotting. This study that 2-DE analysis of S. enteritidis yields useful information concerning S. enteritidis proteome, the results that have been obtained led to a more detailed understanding of Salmonella pathology and open further interesting fields for future work.
朴炳文,李應九 中央醫學社 1971 中央醫學 Vol.20 No.2
A case of arthroplasty of the elbow Ana 25 year old man is presented. Study of clinical symptoms and signs revealed ankylcsis of the left elbow joint in the position of 65 degrees flexion and claw finger deformity in the left hand. Roentgenographic findings showed ankylosis of the left elbow and an old unreduced Monteggia fracture-dislocation. At the first operation the Monteggia fracture dislocation was corrected by open reduction with an autogenous fibular graft and intramedullary nailing for the non-union of the upper third of the ulna and resection of the radial head for dislocation of the head. Eight months later, arthroplasty of the elbow was performed with free fascial interposition. The postoperative course has been satisfactory, any one year after surgery there is 80 degrees(15°-95°) of motion without pain.