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RISS 인기검색어
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- 저자 : 이광문(Kwang-Moon Lee) (검색결과 9 건)
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TMV mRNA 의 in Vitro Translation 을 선택적으로 억제하는 Anti - Sense DNA 및 RNA Oligonucleotide
이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.2
Three different antisense oligonucleotides and their RNA analogues, each complementary to 5`-end and 3`-end sequences of Tobacco Mosaic Virus (TMV) mRNA, inhibit in vitro translation of the genomic RNA in a rabbit reticulocyte lysate. Inhibition depends upon the concentration, binding sites of antisense oligonucleotides and the secondary structure of TMV mRNA. DNA oligonucleotides and their RNA analogues complementary to 5`-end regions of TMV RNA have the same effects on inhibition of translation. However, oligonucleotides complementary to 3`-end TMV RNA regions, which are supposed to form pseudoknotted RNA structure, have different effects on inhibition of translation. When DNA antisense oligonucleotides are used for inhibition of translation in wheat germ extract containing RNase H, then they have higher efficiency for inhibition of translation compared to rabbit reticulocyte lysate. We conclude that the design of antisense oligonucleotide depends on the secondary structure of target genes and their intrinsic components.
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이광문,이소영,서석수,이복률,Lee, Kwang-Moon,Lee, So-Young,Suh, Suk-Soo,Lee, Bok-Luel Korean Society for Biochemistry and Molecular Biol 1991 한국생화학회지 Vol.24 No.5
Tetrahymena human의 Telomere 염기배열을 가지는 DNA, RNA 및 DNA-RNA hybrid oligonucleotide를 다섯 종류 화학 합성하여 전기영동의 gel mobility shift 실험, Tm 측정 및 DMS methylation 반응으로 그들의 구조를 구명하였다. 합성한 oligonucleotide(T1~5) 의 염기배열은 다음과 같다. T1 : d$d(T_2G_4)_4$, T2 : $d(T_2AG_3)_4$, T3: $h(U_2dG_4)_4$, T4 : $h(T_2rG_4)_4$, T5: $r(U_2G_4)_4$. 이들에 대한 nondenaturing PAGE에서 gel mobility 실험결과 T1과 T2는 size marker DNA 24mer보다 훨씬 빠른 gel mobility를 나타내었고, Tm 측정 및 gel mobility 실험결과 T1은 T2보다 안정한 구조를 취하는 것을 확인하였다. 또한, DMS methylation 실험결과 5'말단의 dG가 methylation반응에 sensitive한 것을 확인하였다. 이상의 결과로서 T1 및 T2 oligoneotide는 용액 중에서 Hoogsteen 염기대를 형성하는 Telmere 구조를 취하는 것을 확인하였고. T1 oligoneotide의 염기배열에서 dT를 rU로 치환한 T3는 T1과 동일구조를 취하는 것이 확인되었으나, dG가 rG로 치환된 T4는 T1과는 다른 gel mobility를 나타내었지만, T5와는 동일한 gel mobility를 나타내었다. 이상의 결과로서 분자내 수소결합으로 Hairpin 구조를 취하는 Telomere 구조형성에 가장 중요한 염기는 deoxyguanosine임을 알았다. Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1~5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence $d(T_2G_4)_4$. T2, T3, T4 and T5 were $d(T_2AG_3)_4$, $h(U_2dG_4)_4$, $h(T_2rG_4)_4$ and $r(U_2G_4)_4$, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5'-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer $h(U_2dG_4)_4$, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer $h(U_2dG_4)_4$, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer$r(U_2G_4)_4$ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.
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Telomere 염기배열을 포함하는 DNA , RNA 및 RNA - DNA Hybrid Oligonucleotide 의 구조에 관한 연구
이광문,이소영,서석수,이복률 ( Kwang Moon Lee,So Young Lee,Suk Soo Suh,Bok Luel Lee ) 생화학분자생물학회 1991 BMB Reports Vol.24 No.5
Five kinds of DNA, RNA and DNA-RNA hybrid oligonucleotides (T1∼5) containing Tetrahymena and human telomeric sequences were synthesized. T1 had the sequence d(T₂G₄)₄. T2, T3, T4 and T5 were d(T₂AG₃)₄, h(U₂dG₄)₄, h(T₂rG₄)₄ and r(U₂G₄)₄, respectively. By nondenaturing PAGE, T1 and T2 showed faster gel mobility compared to size marker DNA 24mer in the presence of 50 mM NaCl at low temperatures. T1 showed a more stable telomeric structure than T2 in a Tm analysis experiment and gel mobility shift experiment. We have tested the susceptibility of dGs in telomeric structure to methylation by DMS. At low temperatures, 5`-terminal dG residue of T1 oligomer was hypersensitive to methylation, while other three dGs were resistant. These results suggest that T1 and T2 oligonucleotides form a telomeric structure containing Hoogsteen base pairs. T3 oligomer h(U₂dG₄)₄, where dT residues were substituted by rU, had the same mobility as T1 in nondenaturing PAGE. But T4 oligomer h(T₂rG₄)₄, where dG residues were substituted by rG, showed a different gel mobility compared with T1. T5 oligomer r(U₂G₄)₄ which contained the ribonucleosides only migrated to the same position as T4. These results show that telomeric DNA structure is dependent on dG to form intramolecular hydrogen bonding in the hairpin structure.
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이소영,이광문,서석수,송영환,이복률 ( So Young Lee,Kwang Moon Lee,Suk Soo Suh,Young Hwan Song,Bok Luel Lee ) 생화학분자생물학회 1992 BMB Reports Vol.25 No.1
When channel catfish was injected with Cd and Zn chloride, the liver of the Cd-treated channel catfish was transiently activated to synthesize metallothionein(MT) mRNA and MT-like proteins. The poly(A)^+ mRNA of about 400 bases long was detected in the Cd-treated channel catfish liver with synthetic DNA probes (27 mer), which were conserved sequences in rainbow trout MT cDNA and human MT cDNA, by northern blot hybridization experiment. Amount of the mRNA from Cd treated channel catfish liver was enough to detect translation products in vitro, using the rabbit reticulocytelysate cell-free translation system. These translation products were MT-like proteins, which were about 6.5 kD in size on SDS-PAGE.
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박성은(Seong-Eun Park),서승호(Seung-Ho Seo),김은주(Eun-Ju Kim),이광문(Kwang-Moon Lee),손홍석(Hong-Seok Son) 한국식품영양과학회 2017 한국식품영양과학회지 Vol.46 No.7
본 연구에서는 새싹보리를 첨가하여(1, 2%) 스트링치즈를 제조하고 품질 특성을 검토하였다. pH는 새싹보리를 첨가한 스트링치즈에서 낮은 값을 보였으며 첨가 농도에 따른 유의적 차이는 보이지 않았다. 아미노산 함량은 새싹보리를 첨가함에 따라 threonine, cysteine, tyrosine, arginine, alanine, proline, lysine, isoleucine, leucine, glutamic acid 등의 함량이 비례적으로 증가하였고, 총 아미노산 함량도 대조구에 비하여 새싹보리 2% 첨가 치즈에서 높았다. 색도는 L<SUP>*</SUP>(명도)와 a<SUP>*</SUP>(적색도)에서 새싹보리 첨가량이 증가함에 따라서 낮아지는 경향을 보였으며, b<SUP>*</SUP>(황색도)는 증가하는 경향을 보였다. 물성의 경우 새싹보리 첨가량이 증가할수록 경도와 검성, 씸힙성은 유의적으로 높게 나타났으며, 반대로 응집성은 낮아지는 경향을 보였다. 또한, 신전성 측정 결과 새싹보리를 첨가하지 않은 스트링치즈보다 새싹보리 첨가 스트링치즈의 신전성이 증가함을 확인하였다. 총 페놀 함량은 새싹보리 2% 첨가 치즈에서 유의적으로 높은 값을 보였으며, 총 플라보노이드와 DPPH 라디칼 소거능은 유의적 차이를 보이지 않았다. The objective of this study was to evaluate the quality characteristics of string cheese with different concentrations (0, 1, and 2%) of barley sprout (Hordeum vulgare L.) powder. The pH of barley sprout string cheese (6.26) was significantly lower compared to that of the control (6.46). Chromaticity tended to decrease with increasing amounts of barley sprout powder in terms of L<SUP>*</SUP> (lightness) and a<SUP>*</SUP> (redness), whereas b<SUP>*</SUP> (yellowness) tended to increase. In the texture profile analysis, string cheese prepared with barley sprout powder showed higher values of hardness, gumminess, and chewiness but lower value of cohesiveness. The string cheese prepared with barley sprout powder also showed higher values for total amino acids and stretchability. Total polyphenol contents of string cheese showed the highest value when barley sprout powder was 2%. Therefore, addition of barley sprout powder to string cheese can be valuable for improving quality of string cheese.
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김정현(Jung Hyun Kim),김민영(Min Young Kim),권은경(Eun Kyung Kwon),이광문(Kwang Moon Lee),최돈웅(Don Woong Choi) 대한약학회 2015 약학회지 Vol.59 No.3
Analytical method for related substances can be categorized into two methods depending on the necessity of reference standard (RS). The analytical method of related substances with RS is fast and accurate, but it’s very expensive and technically difficult to synthesize RS due to their complicated structure. Another method is using relative retention time (RRT) and relative response factor (RRF) which are already validated with RS. Validation of this method is not easy and time consuming, but once it has been developed, it can save cost and time. In this study, we developed the analytical method for related substances of fenofibrate using RRT and RRF. We validated the method by evaluating specificity, linearity, accuracy and precision according to the 「Manual for Guideline Application for Validation of Analytical Procedures」 of MFDS. Also, we calculated RRT and RRF between fenofibrate and fenofibrate related substances. The results of this study showed high specificity for fenofibrate and fenofibrate related substances. Correlation coefficient(r) of all substances were more than 0.99, and the recovery of fenofibrate, fenofibrate related substance I, II and III were 99.44%, 100.84%, 99.14% and 101.58%, respectively. Precision of fenofibrate and its related substances were ranged between RSD 0.29% and 0.93%. Quantification limits of fenofibrate, fenofibrate related substance I, II and III were determined to be 0.03 μg/ml, 0.05 μg/ml, 0.04 μg/ml and 0.02 μg/ml, respectively by confirming signal to noise ratio of each chromatogram. The RRT for fenofibrate related substance I, II and III were determined to be 0.35, 0.41 and 1.34, respectively. Also, the RRF for fenofibrate related substance I, II and III were determined to be 1.28, 0.98 and 0.79, respectively. The developed method was applied to determine contents for fenofibrate related substances in commercial fenofibrate (active pharmaceutical ingredient). As a result, developed analytical methods of related substances will be used for revising the monograph of fenofibrate in Korean Pharmacopoeia revision and contribute quality control of drugs by improving cost and time consuming problem of RS.
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