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Dominant Negative C-myb 유전자치료에 의한 두경부종양 세포의 Apoptosis 유도
황평한,윤희완,박민영,양윤수,이호근,이대열,홍기환 대한이비인후과학회 2003 대한이비인후과학회지 두경부외과학 Vol.46 No.8
Background and Objectives:Although the role of c-myb in head and neck tumor has not been studied, aberant expresion of c-myb in various cancer has been demonstrated recently, suggesting that c-myb may play a role in tumorigenesis. Consequently, disrupting c-myb function might prove a possible stratergy for controlling cancer cell growth. The purpose of this study is to investigate the posibility of clinical application of adenovirus-mediated dominant negative c-myb (DN-myb) gene therapy in head and neck cancer. Materials and Method:neck tumors and were assayed the expresion of c-myb and bcl-2 in tumor and normal tissue by RT-PCR. We have generated a replication-deficient recombinant adenovirus encoding the dominant negative c-myb (Ad/DN-myb) or control adenovirus enco-ding no transgene (Ad/GFP) and infected adenovirus to SNU-1076, head and neck tumor cell line. We examined cell proliferation by 3H-thymidine assay, apoptosis by DNA fragmentation, bcl-2 expresion and Akt/PKB phosphorylation by Western imunob-IGF-II, VEGF mRNA expresion by RT-PCR. Results:c-myb expression of head and neck tumor tisues was significan-tly higher than that of normal tisue, indicating that these genes may play an important role in carcinogenesis of head and neck tumor. Ad/DN-myb infected SNU-1076 cells decreased cell proliferation, IGF-1I and VEGF expression, and remarkably increased their apoptosis through the down-regulation of bcl-2 expression and the inhibition of Akt/PKB pathway activation. Conclusion:myb induced apoptosis of head and neck tumor cells and the adenovirusmedia-ted DN-myb gene therapy may be a potential molecular therapy for the treatment of head and neck tumor. (Korean J Otolaryngol 2003 ;46 :677-85)
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홍기환,김성완,윤희완,조윤성,문승현,이상헌 한국음성과학회 2001 음성과학 Vol.8 No.2
The primary sound produced by the vibration of vocal folds reaches the velopharyngeal isthmus and i s directed both nasally and orally. The proportions of the each component i s determined by the anatomical and functional status of the soft palate. The oral sounds composed of oral vowels and consonants according to the status of vocal tract, tongue, palate and lips. The nasal sounds composed of nasal consonants and nasal vowels, and further modified according to the status of the nasal airway, so anatomical abnormalities in the nasal cavity will influence nasal sound. The measurement of nasal sounds of speech has relied on the subjective scoring by listeners. The nasal sounds are described with nasality and nasalization. Generally, nasality has been assessed perceptually in the effect of ma illofacial procedures for Cleft palate, sleep apnea, snoring and nasal disorders. The nasalization is considered as an acoustic phenomenon. Snoring and sleep apnea i s a typical disorders due to abundant velopharyn . The sleep apnea has been known as a cessation of breathing for at least 10 seconds during sleep. Several medical and surgical methods for treating sleep apnea have been attempted. The uvulopalatopharynoplasty(UPPP) involves removal of 1.0 to 3.0 cm of soft palate tissue with removal of redundant orophatyngeal. mucosa and lateral tissue from the anterior and sometimes posterior faucial pillars. This procedure results in a shortened soft palate and a possible risk following this surgery may be velopharyngeal malfunctioning due to the shortened palate. Few researchers have systematically studied the effects of this surgery as it relates to. speech production. Some changes in the voice quality such as resonance (nasality), articulation, and phonation have been reported. In view of the conflicting reports discussed, there remains some uncertainty about the speech status in patients following the snoring and sleep apnea surgery. The study was conducted in two phases: 1) acoustic analysis of oral and nasal sounds, and 2) evaluation of nasality.
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연령에 따른 정상인의 후두 위치 및 발화 기저주파수의 변화에 대한 연구
홍기환,김현기,정경수,윤희완,김성완 대한후두음성언어의학회 1998 대한후두음성언어의학회지 Vol.9 No.1
Changes in the human voice occur between infancy and old age and reflect a myriad of biological changes that influence the size, shape, and physical properties of the larynx. The human larynx is located near the base of the neck and attached inferiorly to the trachea and opens superiorly into the pharynx. The larynx by the third month of fetal life has the same features recognizable at birth. The fundamental frequency of vocal fold vibration generally becomes higher in early age, lower in middle age, and higher in old age. These decreases in Fo undoubtedly result from a combination of factors, consisting of modest increase in length and mass of the muscle and connective tissues of the vocal fold. But the level of the larynx in the neck may be closely connected with Fo directly, high larynx in related with high pitch and low larynx with low pitch. The purpose of this study is to determine the developmental level difference from child to adult larynx using conventional radiography, and the change of speaking fundamental frequency from second decade to sixth decade.
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