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신 이식후 거부반응 환자에서의 In Situ 교잡법을 이용한 Herpesviruses의 검출
심상인,김양리,강진한,유진홍,윤영석,강문원,박인석,방병기,신완식,최영진,장윤식 대한감염학회 1993 감염 Vol.25 No.4
Viral infection would have a deleterious impact on renal graft survival, and increase the morbidity and mortality in renal transplant patients. The clinical diagnosis of viral infection in renal transplant is difficult. Standard procedure for identification of viruses is tissue culture, but it takes much time. Serological diagnosis of viral infection does not always represent the clinically apparent infections. Recently, the value of direct virus detection by nonisotopic DNA probe in situ hybridization (ISH) has been introduced. To evaluate the potential role of cytomegalovirus(CMV), Epstein-Barr virus(EBV) and herpes simplex virus(HSV) on renal graft rejection, eleven rejected kidney allograft specimens were analyzed by ISH with biotinylated DNA probes. CMV-infected lung tissue, EBV-infected lymph node tissue and HSV-infected skin tissues were used as positive controls. One specimen showed positive hybridization with CMV probe and one with EBV probe. The whole procedure can be completed in less than 1.5 hours. In conclusion, we suggest the colorimetric method for ISH could be a powerful tool of detection of viruses in tissues without the need of radioactive probe. Cytomegalovirus, Epstein-Barr virus and herpes simplex virus may be of minor importance in triggering allograft rejection in Korea.