산소흡입이 폐포상피세포에 미치는 영향에 관한 초미세형태학적 연구

정상봉,손태중 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.1

장기 산소호흡이 폐에 미치는 영향을 알기 위하여 가토에 1분당 1.5∼2.0ℓ의 산소를 Ruben valve를 통해 흡입시킨후 24,48,72,96 및 120시간에 나타나는 광학 및 전자현미경적 소견을 요약하면 다음과 같다. 육안적으로는 충혈, 부종, 무기폐, 폐기종, 출혈성 경색 및 출혈등이 관찰되며 이는 산소흡입시간이 길수록 현저한 경향이었다. 광학현미경으로는 무기폐, 부종, 충혈, 염증, 괴사, 소동맥의 비후, 초자막형성, 기관지염, 폐포세포의 비대, 증식, 변성, 박리등이 관찰되는데, 이 현상은 일반적으로는 산소흡입시간이 길수록 현저한 경향이었다. 전자현미경이로는 Ⅱ형 폐포세포에서는 microvilli의 감소 내지 소실, multilamellar body의 감소, 공포화, 전자밀도의 증가, 층판상 구조의 감소 내지 소실 rER의 감소, 수포화, 단열 ribosome의 탈락, Golgi장치의 위축등이 주변화였으며 이 변화는 산소흡입시간이 길수록 현저하였다. 그밖에 Ⅰ형 폐포세포나 대식세포에서는 뚜렷한 소견은 없었다. Ⅱ형 폐포세포에 있어서 multilamellar body의 전단계 구조물이라 추정하고 있는 multivesicular body는 가토에 있어서는 그 형태가 뚜렷하지 않았다. 이상의 연구성적으로 보아 산소를 장시간 흡입하면 가토에 있어서는 폐포표면 활성물질의 생산을 주로 담당하는 Ⅱ형 폐포상피의 multilamellar body를 위시한 세포내 구조물에 변성이 일어나며, 그 정도는 산소흡입시간과 비례한다고 생각한다. For the investigation of morphologic changes of the alveoli influenced by pure oxygen inhalation, the author kept rabbits under inhalation with 1.5-2.0 liter of pure oxygen per minute through the Ruben valve and sacrificed the rabbits at 24, 48, 72, 96, and, 120 hours after inhalation, respectively. The alveoli were observed by light and electron microscopes. The results were summarized as follows; 1. Grossly, there were areas of congestion, edema, atelectasis, emphysema, hemorrhagic infarction and hemorrhage. The longer pure oxygen was inhaled, the more marked these changes were. 2. Light microscopically, congestion, edema, pneumonitis, focal atelectasis, necrosis, thickening of arteriolar wall, hyaline membrane formation, bronchitis, and hyperplasia, hypertrophy, degeneration and shedding of alveolar epithelial cells were observed. These changes were pronounced in proportion to the duration of pure Oxygen inhalation. 3. Electron microscopically, in type Ⅱ alveolar cells the morphologic changes occurred mainly in microvilli; loss or reduction, in muitilamellar bodies; reduction of number, vacuolization, increase of electron density, and reduction or loss of lamellar structures, in rER; reduction of number, fragmentation, dilatation and shedding of ribosomes and in Golgi complexes; atrophy. These changes were also pronounced in proportion to the duration of pure oxygen inhalation. The macrophages and type Ⅰ alveolar cells were rather unremarkable. 4. In type Ⅱ alveolar cells of the rabbits the morphology of multivesicular bodies suspected as a precursory structures of multilamellar body was not evident. 5. From the above results, it may be conceived that inhalation of pure oxygen induces degenerative changes in multilamellar bodies and other intracellular structures of the type Ⅱ alveolar cells. The Severity of degenerative changes is proportioned to the duration of pure oxygen inhalation.

Ethionine 투여로 인한 소장흡수상피세포의 소포체변화

김풍택,손태중,Kim Poong-Taek,Sohn Tae-Joong 한국현미경학회 1973 Applied microscopy Vol.3 No.1

흰쥐를 실험 동물로 하여 기본식에 0.5% 가 되도록 DL- ethionine 을 첨가하여 1 주 내지 4 주간 사육하고 회장 흡수상피세포의 소포체를 중심으로 하여 이와 관련있는 소기관들의 변화를 관찰한 성적을 요약하면 다음과 같다. rER 에 있어서는 attached ribosome 의 탈락과 그 내강의 확장을 free ribosome 에 있어서는 poly- some 의 감소와 monosome 의 증 가를, Golgi complex 에 있어서는 확장을, 그리고 sER에 있어서도 그 내강의 확장 등이 주적인 변화였다. 이 변화들은 ethionine 투여후 2 주째부터 나타나기 시작하여 점차로 성하여지는 경향이 있다. 이상의 연구 성적으로 보아 ethionine 을 투여하면 회장 흡수상피세포에 있어서도 간장이나 취장에서와 같이 단백 합성에 관하여는 소기관들에 주로 변화가 일어난다고 믿어진다. The author studied ed the effect of ethionine upon the absorption epithelium of ileum with particular ettention to the endoplasmic reticulum. Five tenth per cent of DL-ethionine was added to the diet of the experimental group rats and they were sacrificed after 1, 2, 3, and 4 weeks. respectively. The ileum were observed by the electron microscope. The results obtained were summarized as follow: The changes were detachment of membrane bound ribosome, dilatation of endoplasmic reticulum, decrease of polysome with reciprocal increase of monosome, and dilatation of Golgi complex. These changes were occured at 2 nd to 3 rd week from start of ethionine feeding and more severe at fouth week. These observation suggested that ethionine induced degenerative changes of the organelles.

Cycloheximide투여로 인한 췌장외분비세포의 효소원 과립의 동태

정상봉,채종민,손태중 慶北大學校 醫科大學 1980 慶北醫大誌 Vol.21 No.1

흰쥐에 cycloheximide를 체중 100mg당 0.1mg씩을 투여하고 1일, 2일, 3일 및 4일째에 췌장선방세포의 효소원 과립의 동태를 관찰한 성적으로 요약하면 다음과 같다. Cycloheximde를 투여한 후 1일째에는 효소원 과립은 심히 감소하고, 그 내의 전자밀도도 낮아지는 경향이였으나, 2일째 부터는 그 수가 증가하고 그 내의 전자밀도도 높아지며, 이는 4일째가 되며는 거의 회복되는 경향을 나타내었다. 이상의 연구 성적으로 보아 cycloheximide를 투여하면 효소원 과립의 분비와 생성이 다같이 지연되는 것으로 생각된다. The author subcutaneously administered 0.1mg/100gm of body weight of cycloheximide to the rats and studied reactivity of the zymogen granules in the pancreatic acinar cells on the first, second. third. and fourth day after administration respectively. The results were as follow. The zymogen granules in the pancreatic acinar cells were markedly decreased both in number and electron density on the first day after administration of the cycloheximide. From the second day after administration of the cycloheximide the numbers and electron density of the zymogen granules became increase. On the fourth day these changes tended mostly to recover. On the basis of these results it may thought that both the excretion and synthesis of the zymogen granules in the pancreatic acinar cells should be delyed by the cycloheximide.

항원성 자극을 받은 임파절세포의 Rough Surfaced Endoplasmic Reticulum 의 변화

이창수,권세훈,손태중,Lee Chang-Soo,Kwon Sae-Hoon,Sohn Tae-Joong 한국현미경학회 1973 Applied microscopy Vol.3 No.1

토끼의 족서부 피하로 Bovine Gamma Globulin과 Ineomplete Freud's Adjuvant를 주입한 후 국소임파절내의 항체생산 세포인 형질세포의 rER을 경시적으로 관찰한 결과를 요약하면 다음과 같다. (1) 항원투여전의 형질세포의 rER 는 일반적으로 편평낭상 (flattened sac) 을 정하고, 항원 투여 후 3 일째에는 일반적으로 소포상 (vesic lar) 를 그리고 7 일내지 10 일째에는 공포상 (vacuolar) 를 정하고 그 강내에는 intracisternal granule 이 다수 출현하며 20 일째에는 다시 확장낭상 내지는 편평낭상을 정하게 된다. 이상의 연구성적으로 보아 편평낭상을 정하는 형질세포의 rER에 항원성 자극을 가하면 처음에는 소포상으로 되었다가 공포상으로 변화하며, 항원 성자극이 소퇴하면 다시 편평낭상으로 복귀된다고 생각된다. The changes of rough surfaced endoplasmic reticulum of plasma cells in the regional lymph node after injection of Bovine Gamma Globulin summarized as follow: The rough ER of plasma cells before antigenic stimulation shows flattened sac but changed to vesicular at 3rd day and vacuolar at 7th to 10th day. lntradisternal granules are appeared within the lumina or rough ER at 7th to 10th day. The rough ER is changed to distended sac or flattened sac at 20th day. The results suggest that normally flattened sac of rough ER is changed to vesicular and then to vacuolar after antigenic stimulation. But they are returned to distended sac or flattened sac with disappearance of antigenic stimuli.

쥐에서 Cyclophosphamide 투여에 의한 모낭의 초미형태학적 변화

김대연(Dae Yeon Kim),이규석(Kyu Suk Lee),송준영(Joon Young Song),손태중(Tae Joong Sohn) 대한피부과학회 1987 대한피부과학회지 Vol.25 No.1

Authors studied the effects of cyciophosphamide, a potent inhibitor of nucleoprotein synthesis, to ivestigate the morphologic evidence of destructive actions to the hair. follicles. Sprague-Dawley rats were received 4 mg/kg of eyclophosphamide for 1 to 6 weeks, intraperitoneally, a.nd examined light and electron microscopically. Light microscopically, distortion and constriction of the hair shafts, diminished diameter of the ha.ir bulbs, and atrophy of. the hair matri.x were developed from 1 week. which were more prominent in second weeks and they were progressed after that time. Hairs were frequently fractured due to constriction of the hair shaf ts. Electron microscopically, cells of the hair pulp were decreased in number, and cells of the hair matrix were atrophied, Degenerative changes of the cellular organelles participating in grovth. and development of the hairs were noted, such as disordered formation of tricholyaline granules, diffuse atrophy and increased electron density of the inner root sheath, and loss of the glycogen and intercellular edema of the outer root sheath, but basal cells of the matrix showed minor changes relatively. From the above results, cyclophosphamide may specifically alter the matrix cells of the hair follicles and induces anagen hair losses, which may be reversible at a small dosage when the drug is discontinued, because basal cells of the matrix ahow rninor changes.

망막색소상피세포 상해에 의한 Blood - Retinal Barrier 의 변화

김명미(Myong Mi Kim),한덕기(Duk Kee Hahn),손태중(Tae Joong Sohn),곽정식(Jyung Sik Kwak) 대한안과학회 1987 대한안과학회지 Vol.28 No.4

만성 (慢性) 활동성 (活動性) 간염에서 (肝炎) 근섬유아세포의 (筋纖維芽細胞) 동태 (動態)

김호각(Ho Gak Kim),정준모(J.Mo Chung),손윤경(Yoon Kyung Shon),곽정식(Jyung Sik Kwak),손태중(Tae Joong Sohn) 대한소화기학회 1988 대한소화기학회지 Vol.20 No.3

N/A This study has been carried out to investigate the behavior and histologic origin of myofibroblasts, which are able to be found in the liver tissue of chronic active hepatitis. The biopsied liver tissues of patient were examined under the light and electron microscopes and studied through the immunohistochemical method. Through light microscopic examination, the cases were devided into four mild ones, eight moderate ones and four severe ones. On immunohistochemical study, all mild cases show negative findings to antibody to antiactin and positive findings were demonstrated in all moderate and severe ones. On electron microscopic study, myofibroblasts were found in each three cases of moderate and severe ones and they exhibit intermediate forms between smooth muscle cells and myofibroblasts having the hundles of microfilaments, wrinkled nuclei and basement membrane. Most of them are located in nearby areas of portal veins and in the periphery of these areas, proliferation of Ito cells and transformation of them into similar forms of myofibroblasts were noted. These findings suggest that the myofibroblasts are noted in the moderate and more severe cases of chrrniic active hepatitis showing marked fibrosis and they contribute to the fibrosis of portal areas. Besides, it is presumed that myofibroblasts of chronic active hepatitis are originated from Ito cells.

구연발표 : 만성활동성 간염에서 근직유아 세포의 동태

서인수(In Soo Seo),김호각(Ho Gak Kim),곽규식(Kyu Shik Kwak),최용환(Yong Hwan Choi),정준모(Jun Mo Chung),손윤경(Yoon Kyung Shon),곽정식(Jyung Sik Kwak),손태중(Tae Joong Sohn) 대한소화기학회 1987 대한소화기학회 추계학술대회 Vol.1987 No.-

항원성 및 비항원성 자극에 대한 면역적격세포의 동태에 관한 연구

손태중 慶北大學校 醫科大學 1970 慶北醫大雜誌 Vol.11 No.1

간학의 EM 영상 : 전자현미경으로 만나는 간의 모습(7)

손태중 대한간학회 2000 Clinical and Molecular Hepatology(대한간학회지) Vol.6 No.1