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넙치 전 암컷 집단의 생산을 위한 연구 II. 성전환된 자성발생성 2배체 수컷의 자손 검정
방인철,김경길,김윤,김종현,Bang In-Chul,Kim Kyung-Kil,Kim Yoon,Kim Jong-Hyun 한국양식학회 1996 韓國養殖學會誌 Vol.9 No.2
본 연구는 성전환된 자성발생성 2배체 수컷의 정액을 보통 암컷 및 자성발생성 2배체 암컷의 성숙란에 인공수정시킨 후 자손의 성비를 분석하였다. 자성발생성 수컷의 유도는 자성발생성 2배체를 전장 13.5 mm에서 57.6 mm까지 62일간 $17{\alpha}$-methyltestosterone 10 ppb의 농도로 침지 처리하여 유도하였다. 자손 검정 결과, 보통 암컷과 자성발생성 수컷 교배군의 암컷 유도율은 평균 $37.4\%$로 낮게 나타났다. 그러나 자성발생성 2배체 암컷과 자성발생성 수컷 교배군에서는 8개 실험군 중 6개 실험군에서 $100\%$ 암컷이 유도 되었고 2개 실험군에서는 $96.9\%$ 및 $95\%$ 암컷이 유도되어, 전체 $99\%$ 의 암컷 유도율을 나타내었다. 이러한 결과는 자성발생성 2배체 암컷과 성전환된 자성발생성 2배체 수컷이 정상적인 생식 기능을 통해 교배되어 전 암컷 집단을 생산할 수 있음을 시사한다. A study was carried out to test sex ratio of the progeny that were induced by the artificial fertilization between normal female and gynogenetic diploid eggs and sex-reversed gynogenetic diploid male sperm. Sex reversed gynogenetic diploid males were produced successfully in gynogenetic diploid female by immersion treatment with $17{\alpha}$-methyltestosterone at the concentration of 10 pub from $13.5{\pm}0.22$ mm to $57.6{\pm}5.1$ mm in total length. In $F_1$ progeny test, matings between normal females and sex-reversed gynogenetic males were not produced all female, but matings between gynogenetic diploid females and sex-reversed gynogenetic males were produced all female populations in six out of eight groups and two lots produced $96.9\%$ and $95\%$ female. These data showed that all-female populations were produced by crossing between gynogenetic diploid female and sex reversed gynogenetic diploid male.
넙치 3계통의 혈액성분 분석 및 계통간 교배군의 초기성장과 생존율
방인철,박상용,이윤아,홍경표,김종형 순천향대학교 기초과학연구소 2003 순천향자연과학연구 논문집 Vol.9 No.2
Hematological characteristics of the three populations in the olive flounder, Paralichthys olivaceus were analyzed. The sample fishes were collected from coastal sea areas around Korea (east and west coastal wild populations) and Japanese cultured population. Result researched into Hemoglobin, PCV, total protein, glucose, GOT and GPT in the three population were not significant (P>0.05). Abnormality of mating groups used east female were higher than Japanese female (P<0.05), but no significant differences were found in fertilization rate and hatching success (P>0.05). Japanese X east group showed highest percent and east X west group showed lowest percent in survival rate, significantly (P<0.05). East X east, Japanese X west and Japanese X Japanese groups showed higher growth rate in total length and body weight than the other groups, however, east X west group showed lower growth, significantly(P<0.05).
방인철 순천향대학교 기초과학연구소 1999 순천향자연과학연구 논문집 Vol.5 No.1
A growth study was conducted to investigate the effect of stocking density on performance of juvenile spotted sea bass (Lateolabrax sp.). Fish averaging 25g were stocked into duplicate tanks (50ℓ each) at four different densities of 250g, 500g, 1,000g, and 1,500g/100ℓ, and fed commercial diet (extruded pellet) for 2 months. Weight gain was decreased as density increased, body weights of fish stocked at 250∼500g/100ℓ were higher than those of higher density (p<0.05). Feed efficiency was increased as density increased at 250∼1,000g/100ℓ initial stocking density, but it was decreased at lowest density. These results indicate that optimal stocking density of spotted sea bass juvenile is 1∼1.5kg/100ℓ final stocking density for indoor tank culture system.
NANOTECHNOLOGY FOR ADVANCED NUCLEAR THERMAL-HYDRAULICS AND SAFETY: BOILING AND CONDENSATION
방인철,정지환 한국원자력학회 2011 Nuclear Engineering and Technology Vol.43 No.3
A variety of Generation III/III+ water-cooled reactor designs featuring enhanced safety and improved economics are being proposed by nuclear power industries around the world in efforts to solve the future energy supply shortfall. Thermal-hydraulics is recognized as a key scientific subject in the development of innovative reactor systems. Phase change by boiling and condensation in the reverse process is a highly efficient heat transport mechanism that accommodates large heat fluxes with relatively small driving temperature differences. This mode of heat transfer is encountered in a wide spectrum of nuclear systems,and thus it is necessary to determine the thermal limit of water-cooled nuclear energy conversion in terms of economic and safety. Such applications are being advanced with the introduction of new technologies such as nanotechnology. Here, we investigated newly-introduced nanotechnologies relevant to boiling and condensation in general engineering applications. We also evaluated the potential linkage between such new advancements and nuclear applications in terms of advanced nuclear thermal-hydraulics.