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Streptomyces subrutilus P5가 생산하는 철 함유 superoxide dismutase의 분비
박재승,김재헌,Park, Jae-seung,Kim, Jae-heon 한국미생물학회 2015 미생물학회지 Vol.51 No.2
본 연구에서는 Streptomyces subrutilus P5의 생장과 세포내 외 철 함유 superoxide dismutase 활성을 비교 분석하여 철함유 superoxide dismutase의 분비 시점을 확인하고 분자 수준에서 이 효소의 분비에 관여하는 유전정보를 확인하고자 하였다. Streptomyces subrutilus P5의 균체 생장은 건체 중량을 측정하여 결정하였다. Glucose는 log phase에서 급격히 소모되어 24시간 후에 이르러 완전히 고갈되었다. 세포내의 철 함유 superoxide dismutase는 배양 후 3시간에 나타나며 세포외 철 함유 superoxide dismutase는 배양 후 7.5시간부터 나타난다. 따라서 superoxide dismutase는 용균에 의해서가 아니라 능동적인 분비기작에 의해서 세포 외로 분비된 것으로 추측할 수 있다. Streptomyces subrutilus P5의 sodF에는 signal peptide 유전정보가 존재하지 않았다. 그러나 sodF의 상류지역에서 다른 세균의 type III 분비단백질 유전자와 유사한 type III 분비상자가 발견되었다. Streptomyces 균주에서 type III 분비단백질이 존재할 가능성이 있음을 처음으로 제시하였다. We tried to analyze the growth time for secretion of the iron containing superoxide dismutase by comparing the intra-and extracellular enzyme activity from Streptomyces subrutilus P5 and analyze possible genetic information for this enzyme secretion. The mycelial dry weights and glucose concentrations in culture filtrates were determined during growth. Glucose was consumed rapidly during logarithmic growth phase and almost exhausted at 24 h of cultivation. While the intracellular activity of iron containing superoxide dismutase was first appeared at three hours, the extracellular activity of this enzyme appeared from 7.5 h of cultivation, early logarithmic growth phase. This early presence of the superoxide dismutase might not be the result of cell lysis but active secretion pathway. There was no information for signal peptide responsible for the enzyme secretion in sodF. However, we found a type three secretion box in the promoter region of sodF that has been known for the genes of type III secreted proteins in other bacteria. This is the first report on the possible existence of type III secretion in Streptomyces.
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박재승 ( Jae Seung Park ) 한국외국어대학교 언어연구소 2005 언어와 언어학 Vol.0 No.35
The purpose of this study is to establish the chinese-korean machine translation system. The study of the machine translation has received relatively little attention from korean scholarship in general, what was worse, that is much neglected branch of chinese linguistics in korea. So far. very little has been done in this direction. In China, this topic of the study has been studied extensively during the past five decades. The generation of sentence. till now. has mainly examined in syntactic dimension. That is to say, throughout these studies runs a common theme: the algorithm of machine translation system must be based on Chom`s rewriting rules. However, it cannot be satisfactorily explicated by syntax analysis. Because the sentence is not generated from the combinations between the syntactic categories. The structure of sentence is strong association with cooccurrence restrictions. Therefrore, we need more concrete and delicate the cooccurrence restrictions such as collocational restrictions. My approach in this paper is based on collocational restrictions and semantic informations. First, we pointed out the importance of punctuation in analyzing the structure of chinese sentence. Second. in considering language dependent phenomena. we would rather use morphologidcal alalysis than use word segmentation. This kind of processing may all the more possible because Chinese sentence has no word delimiter. Third Qi hu YAng`s (2000) study introduces the established researches on disambiguation. His approach is based on the principle of "preference". This principle seems plausible, yet it requires further examination. THe purpose of my paper is to an alternative approach which might present the method in solving the question. Four, we introduce some kind of method for establishing machine readable dictionary. we find out the method for dealing with equivocation. name and classify the collocational constructions. This study is only a first step. we believe it offers some insight into the problems. There are, however, a number of problems that remain to be explpred. The study concludes by mentioning some of the further problems raised by this approach to spreading activation model.
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박재승(Park Jae-seung) 한국중어중문학회 2008 中語中文學 Vol.43 No.-
This work was motivated by the need for an efficient item-weight-assignment and the need that a test result makes a sense to an examinee which well accords with the purpose of a given test. In this paper, we showed empirically that classical test theory served the needs up to some level and in the same context, we pointed out that score approach may not suffice the needs. Vocabulary is the most fundamental element to get the abilities of using chinese correctly and effectively. The most basic element which composes an individual's vocabularies is to know the meaning of each word clearly. One word has its own basic meaning and also has a relative meaning that depends on the relation of another word. Therefore the right vocabulary teaching should be like this : Learners are able to know the word's basic meaning and related meaning correctly enough to use it in a context in a right way. Each result was presented in numerical value as a statistical data and was analyzed. As the result I confirmed this synonym teaching method is more effective one to grasp the difference of the meaning existed between synonyms. As a matter of facts, the study in this thesis doesn't get to suggesting a perfect vocabulary teaching model, for this study was done in restricted conditions. But by proposing the point of views and the method the synonym teaching has to get, it seems to obtain some results which raise the necessities to reconsider the directions and the ways the future Chinese vocabulary teaching has to pursue.
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박재승 ( Jae Seung Park ) 한국외국어대학교 중국연구소 2011 中國硏究 Vol.52 No.-
Beginning with the analysis of the problems that the chinses proficiency testing has brought about in recent years. the purpose of this paper is explore the rather reliability and test validity of FLEX developed by Hankuk University of foreign studies. This study investigates the potential roles in the Generalizability theory in the validation of a performance-based chinses test. Data for this study come from Hankuk University of foreign studies`s FLEX. There is no doubt that the FLEX reform will change the traditional chinese way of teaching, teachers` dominating the classes by explaining the language points. Therefore, teachers should pay more attention to the formative test, such as diagnostic tests as the various learning periods, so that the teaching plans and methods can be adjusted in time.
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박재승 ( Park Jae-seung ) 현대일본학회 2017 日本硏究論叢 Vol.46 No.-
본고는 전후 일본의 교육과정 중에서 ‘국어과’, 즉 자국어 교육과정에 대해 살펴보았다. 교육과정 가운데도 특히 ‘국어과’ 교육은 사회생활에서 의사소통을 하고 사회구성원들을 결속하는데 매우 중요한 과목이다. 일본의 ‘국어과’ 교육과정인 학습지도요령의 변천을 살펴본 결과 본고에서는 다음과 같은 특징을 알 수 있었다. 첫째, 일본의 ‘국어과’ 교육과정은 명칭의 변화가 거의 없다는 점이다. 이 점은 교육과정이 바뀔 때마다 과목명이 바뀌는 한국의 실정과 다른 점이다. 교육과정은 교과서를 만드는 데 중요한 지침이 될 뿐만 아니라 국어교육의 방향과 내용을 알려주기 때문에 매우 중요하다. 따라서 교육과정은 가르치는 교사는 물론이고 학생들도 이해할 수 있도록 과목명이 그대로 유지되는 것이 필요하고 내용 서술도 쉽게 하는 것이 중요하다고 할 것이다. 둘째, 일본의 교육과정은 언어활동을 매우 중시한다는 점이 드러났다. 언어활동은 단지 ‘국어과’에서 뿐만 아니라, 다른 교과에서도 강조되고 있다. 언어능력은 사회생활을 원활하게 할 수 있고, 사회구성원들 간에 결속을 다지는 데에도 매우 중요한 역할을 한다는 점을 감안할 때 모든 교과에서 언어활동을 강조하는 것은 매우 유익하다고 할 것이다. 셋째, 일본 교육과정에서 특이한 것 중 하나는 한자교육을 중시하고 있다는 점이다. 일본 언어생활을 살펴보면 한자를 모르면 국어생활을 제대로 할 수 없다. 따라서 사회생활이나 학문연구를 위해서도 한자교육은 선택이 아니라 필수가 될 수밖에 없다. 이렇게 특이한 사정이 있어 일본 교육에서 한자를 중시하게 된 것이다. 넷째, 일본의 교육과정의 변화를 살펴보면 시대의 변화를 매우 발 빠르게 반영했음을 알 수 있다. 시대변화에 능동적으로 대응하고 이를 반영하여 교육하는 것은 국가발전에 매우 도움이 될 것이라고 생각한다. 일본의 교육과정과 ‘국어과’ 개정의 과정을 살펴보면서 일본이 시대에 잘 적응하기 위한 교육을 하기 위해 고심해 왔음을 알 수 있었다. The purpose of this article is to review the changes of Japanese curriculum in Japan after the second world war. Japan has become the world's second largest economy after 30 years of defeat in World War II. Among the curriculums, Japanese education in Japan has been a very important subject in social life. As a result of the review in this article, the following results were obtained : First, the Japanese language and curriculum in Japan have little change in its name. The curriculum is very important because it is important to teach the direction and contents of Japanese language education as well as to make it important to produce textbooks. Secondly, it turned out that language activity is very important in the curriculum. Language activity is emphasized not only in terms of language but also in other subjects. It is very helpful to emphasize linguistic activity in all subjects, given that it is important to facilitate social life and play a vital role in fostering solidarity among members of society. Third, one of the strangest things in the Japanese curriculum is that it focuses on Chinese character education. If you look at Japanese language life, you can not speak Japanese properly if you do not know Chinese characters. Therefore, for the sake of social life or academic studies, Chinese character education is indispensable, not choice. Such peculiar circumstances have led to the importance of Chinese characters in Japanese education. Fourth, if you look at the changes in the curriculum of Japan, you can see that the changes reflect the changing times very quickly. It is necessary for the educational object to actively respond to changes in the times and reflect the social changes accordingly.
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박재승(Park, Jae-seung) 한국중어중문학회 2020 中語中文學 Vol.- No.79
This paper has reorganized the obligation modality category and we developed the method of analyzing the distinctive functions of ‘必须,要,得,应该’ in each functional category. As a first step, we set up a basic characteristic shared by the members of the category. Distinguish between ′Subjective and objective′ and ′Speaker′s behavior control power′, which is used as a concept in the existing research. In the second step, we presented ′可以′ which is selected as the boundary mark of categories, is how to be classified into ′the obligation modality category′ and ′permission modality category′. As a third step, We presented the distinctive function of the members of the obligation modality category.
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