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오미자칠해목 추출물의 과산화수소로 유발된 PC12뇌세포 사멸과 스코폴라민으로 유발된 렛드 동물모델에 대한 개선 효과
박은국,한경훈,허재혁,김남기,배문형,서영하,용윤중,정선용,최춘환,Park, Eun-kuk,Han, Kyung-Hoon,Heo, Jae-Hyeok,Kim, Nam-Ki,Bae, Mun-Hyoung,Seo, Young-Ha,Yong, Yoon-joong,Jeong, Seon-Yong,Choi, Chun-Whan 한국식품영양학회 2020 韓國食品營養學會誌 Vol.33 No.3
Cognitive impairment is considered to be key research topics in the field of neurodegenerative diseases and in understanding of learning and memory. In the present study, we investigated neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts in hydrogen peroxide-induced neuronal cell death in vitro and scopolamine-induced cognitive impairment in Sprague Dawley<sup>®</sup> (SD) rat in vivo. Apoptotic cell death in neuroblastic PC12 cell line was induced by hydrogen peroxide for 1 hour at 100 μM. However, mixture of SC and RF treatment prevented peroxide induced PC12 cell death with no neurotoxic effects. For in vivo experiment, the effect of SC and RF extracts on scopolamine-induced cognitive impairment in SD rat was evaluated by spontaneous alternation behavior in Y-Maze test. After 30 min scopolamine injection, the scopolamine-induced rats presented significantly decreased % spontaneous alteration and acetylcholine level, compared to non-induced group. However, treatment of SC+RF extracts rescued the reduced % spontaneous alteration with acetylcholine concentration from hippocampus in scopolamine-induced rats. These results suggested that mixture of SC and RF extract may be a potential natural therapeutic agent for the prevention of cognitive impairment.
과산화수소로 유도된 SH-SY5Y 신경세포 사멸에 대한 오미자·칠해목 추출혼합물의 보호효과
박은국,한경훈,이승희,김남기,배문형,서영하,용윤중,정선용,최춘환,Park, Eun-kuk,Han, Kyung-Hoon,Lee, Seung-Hee,Kim, Nam-Ki,Bae, Mun-Hyoung,Seo, Young-Ha,Yong, Yoon-joong,Jeong, Seon-Yong,Choi, Chun-Whan 한국식품영양학회 2018 韓國食品營養學會誌 Vol.31 No.6
In neuronal cell deaths, oxidative stress is normally implicated with a most of these deaths occurring in neurodegenerative disorders such as the Alzheimer's and Parkinson's diseases. In this study, the neuroprotective effects of Schisandra chinensis (SC) and Ribes fasciculatum (RF) extracts on hydrogen peroxide ($H_2O_2$)-induced oxidative stress in neuroblastic cell line were investigated. For an hour, hydrogen peroxide of $100{\mu}M$ concentration, was induced on neuroblastic cells, causing apoptic cell death. For the neuroprotection, a sample of neuroblastic cells had been pre-treated with SC and RF extracts for 24 hours before application of the hydrogen peroxide. No neurotoxic effects were observed in the cells that had been treated by SC and RF. This prove that the treatment of SC and RF extract prevented apoptotic cell death of neuroblastic cell line exposed to oxidative injury. In addition, applying both SC and RF extracts at a 7:3 ratio increased the neuronal cell survival rate, compared to individual treatments of SC and RF extract. This study suggests that SC and RF extracts may be potential therapeutic agents for the prevention of neuronal cell death.
Ribes속 식물 감별을 위한 UHPLC-HR-MS/MS 기반 대사체학 접근
김진아,최윤혁,이종석,정선용,박은국,배문형,서영하,최춘환 한국약용작물학회 2018 한국약용작물학술대회 발표집 Vol.2018 No.10
Background : Ribes fasciculatum var. chinense Maxim. has been traditionally used as febrifuge, diuretic and antidote against urushiol in Korea. This study described a metabolomics approach used to discriminate the genus Ribes from different sources. Methods and Results : Four different types of Ribes from Korea were analyzed by ultra-high-resolution mass spectrometry (UHPLC-HR-MS/MS) based metabolomics. Multivariate statistical method such as principal component analysis (PCA) were used to compare the derived patterns among the samples. The data set was subsequently applied to various metabolite selection methods for sophisticated classification with the optimal number of metabolites. The results showed variations in accuracy among the classification methods for the samples of different origins, especially for cultivation region. Conclusion : This proposed analytical method coupled with multivariate analysis is fast, accurate, and reliable for discriminating the origin of the genus Ribes samples and is a potential tool to standardize quality control in the genus Ribes related products.
Bo-Young Kim,Do-Wan Kim,박은국,Jeonghyun Kim,이창근,Hyun-Seok Jin,Seon-Yong Jeong 대한의학유전학회 2022 대한의학유전학회지 Vol.19 No.2
Purpose: Osteoporosis is a common calcium and metabolic skeletal disease which is characterized by decreased bone mass, microarchitectural deterioration of bone tissue and impaired bone strength, thereby leading to enhanced risk of bone fragility. In this study, we aimed to identify novel genes for susceptibility to osteoporosis and/or bone density. Materials and Methods: To identify differentially expressed genes (DEGs) between control and osteoporosis-induced cells, annealing control primer-based differential display reverse-transcription polymerase chain reaction (RT-PCR) was carried out in pre-osteoblast MC3T3-E1 cells. Expression levels of the identified DEGs were evaluated by quantitative RT-PCR. Associa-tion studies for the quantitative bone density analysis and osteoporosis case-control analysis of single nucleotide polymor-phism (SNPs) were performed in Korean women (3,570 subjects) from the Korean Association REsource (KARE) study cohort. Results: Comparison analysis of expression levels of the identified DEGs by quantitative RT-PCR found seven genes, Anxa6, Col5a1, Col6a2, Eno1, Myof, Nfib, and Scara5, that showed significantly different expression between the dexamethason-treated and untreated MC3T3-E1 cells and between the ovariectomized osteoporosis-induced mice and sham mice. Associa-tion studies revealed that there was a significant association between the SNPs in the five genes, ANXA6, COL5A1, ENO1, MYOF, and SCARA5, and bone density and/or osteoporosis. Conclusion: Using a whole-genome comparative expression analysis, gene expression evaluation analysis, and association analysis, we found five genes that were significantly associated with bone density and/or osteoporosis. Notably, the associa-tion P-values of the SNPs in the ANXA6 and COL5A1 genes were below the Bonferroni-corrected significance level.
아밀로이드 베타로 유도된 신경세포 사멸에 대한 PineXol<sup>®</sup>의 보호효과
한경훈 ( Kyung-hoon Han ),이승희 ( Seung-hee Lee ),박광성 ( Kwang-sung Park ),송관영 ( Kwan-young Song ),김정희 ( Jung-hee Kim ),박은국 ( Eun-kuk Park ),한성희 ( Sung-hee Han ) 한국식품영양학회 2017 韓國食品營養學會誌 Vol.30 No.6
Amyloid-β protein (Aβ) is known to increase free radical production in neuronal cells, leading to cell death by oxidative stress. The purpose of this study was to evaluate the protective effects of PineXol<sup>®</sup> on Aβ<sub>25-35</sub> induced neuronal cell death. Rat pheochromocytoma (PC-12) cells were pre-treated with 100 μg/mL of PineXol<sup>®</sup> for 2 h. The cells were exposed to single dose of 30 μM Aβ<sub>25-35</sub> for 24 h. Cell death was assessed by a cell count kit-8 (CCK-8) assay, lactate and dehydrogenase (LDH) release assay. An Apoptotic process was analyzed by a protein expression of the Bcl-2 family using western blotting. Cell viability increased in PC-12 cells treated with both Aβ<sub>25-35</sub> and PineXol<sup>®</sup>, compared to the control group. PineXol<sup>®</sup> induced a decrease of the Bcl-2 protein expression (p<0.05), while Bax and Sod1 increased (p<0.05), indicating attenuation of Aβ<sub>25-35</sub> induced apoptosis. These results suggest that PineXol<sup>®</sup> may be a good candidate for the prevention of Alzheimer’s disease(AD).