검체채취실의 고객만족도 향상

박연보 ( Youn Bo Park ),강희정 ( Hee Jung Kang ),권흥만 ( Hung Man Kwon ),안상진 ( Sang Jin Ahn ),양석환 ( Suk Hwan Yang ),태연주 ( Yeun Ju Tae ),진영희 ( Young Hee Chin ),조현구 ( Hyon Koo Jo ),이복자 ( Bok Ja Lee ),구선회 ( Sun 대한임상검사과학회 2004 대한임상검사과학회지(KJCLS) Vol.36 No.2

The sample collection room(SCR) will have much more influence than all the other departments for the improvement of hospital image, if anyone coming to the SCR in the hospital goes back with the perfect complacency and because most clients who have much stresses and fatigues pay a final visit to the SCR via receipt-diagnosis- acceptance process. SCR has improved its image for the purpose of gratifying clients, in order for clients to visit the hospital again, the quality improvement(QI) team in the Diagnosis Inspection Medical Department has come to a conclusion as follows. The degree of client gratification before improvement marks 65.9 point, but the degree after improvement was 74.2 point. Therefore, satisfaction has increased by 8.3 points. The degree of client gratification in groups before improvement marks (1) service parts-89.2 points (2) facilities and environments-49.1 point (3) toilet facilities-46.3 point. But its gratification after improvement marks (1) 92.5 point (2) 60.1 point (3) 61.0 point. Therefore the degree of satisfaction has increased by (1) 3.3 point, (2) 11.0 point, (3) 14.7 point. The progress of facility improvement plans and the exclusion of improvement on the facility contents in the hospital have made facilities and environments of SCR and toilet facilities to be poorly improved. Although service parts have a good mark, and the facilities and environments are not scoring well, the whole degree clients`` gratification of SCR couldn``t be helped by the low grade. Therefore the bottom line for the clients`` gratification of SCR in the future is to ameliorate the facilities and environments. SCR will take the clients`` gratification survey every year and if any items get low marks, that is, below 90 point throughout the survey, SCR will immediately starts the improvement work for the clients`` gratification with operating the programs of controlling quality continually, and SCR should induce the operation of services, participating in the kind campaign drive for clients. So SCR will adopt the incentive system for the best staff members who perform these kinds of services.

항산화제 또는 팜유로 보강된 유채유를 이용한 라면의 산화안정성

박연보(Yun-Bo Park),박혜경(Hye-Kyung Park),김동훈(Dong-Hoon Kim) 한국식품과학회 1989 한국식품과학회지 Vol.21 No.4

곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 향기성분 비교

연보람(Bo-Ram Yeon),조해미(Hae Me Cho),윤미선(Mi Sun Yun),주진우(Jin-Woo Jhoo),정지욱(Ji Wook Jung),박유화(Yu Hwa Park),김성문(Songmun Kim) 한국식품영양과학회 2012 한국식품영양과학회지 Vol.41 No.12

본 연구의 목적은 곰취(Ligularia fischeri)와 한대리곰취(Ligularia fischeri var. spicifoprmis) 정유의 향취 및 화학성분을 비교하는데 있다. 곰취와 한대리곰취의 정유 함량은 각각 0.12%와 0.04%였으며, 역치수준은 각각 0.01%와 0.1%였다. 향수개발전문가에 의해 평가된 곰취 정유의 향취는 citrus, coniferous, green, oily, smoky, wet하였던 반면 한대리곰취 정유의 향취는 balsam, earthy, oily, wet하여 매우 상이하였다. 곰취 정유에는 총 19종의 화학성분이 검출되었는데 탄화수소가 14종(75%), 알코올이 4종(20%), 아세테이트가 1종(5%)이었으며, 주된 화학성분은 L-β-pinene(36.02%), D-limonene(25.64%), α-pinene(24.85%), β-phellandrene(5.39%), 3-carene(3.77%), α-phellandrene(2.05%)이었다. 한대리곰취 정유에는 총 25종의 화학성분이 검출되었는데 탄화수소 17종(68%), 알코올 4종(16%), 아세테이트 3종(12%), 질소화합물 1종(4%)이었으며, 주된 화학성분은 D-limonene(39.80%), L-β-pinene(35.43%) α-pinene(11.94%), 3-carene(3.70%), β-cubebene(3.51%), β-phellandrene(2.38%)이었다. 한대리곰취 정유에서는 ρ-cymene, γ-muurolene, γ-cadinene, germacrene D, ingol 12-acetate, butyl 9,12,15-octadecatriene과 nimorazole이 미량 검출되었으나, 곰취 정유에서는 이러한 화학성분들이 검출되지 않았다. 본 연구의 결과 각각 독특한 향취를 갖는 곰취와 한대리곰취 정유는 향장산업제품 개발을 위한 소재로 활용될 수 있을 것이라 판단된다. This study was conducted to compare fragrance and volatile chemicals of essential oils in Gom-chewi (Ligularia fischeri) and Handaeri Gom-chewi (Ligularia fischeri var. spicifoprmis). Essential oils were extracted by steam distillation of leaves of Gom-chewi (GC) and Handaeri Gom-chewi (HGC), after which samples were collected by solid-phase micro extraction and the compositions of the essential oils were analyzed by gas chromatography- mass spectrometry (GC-MS). The yields of the essential oils in GC and HGC were 0.12% and 0.04%, respectively, and the threshold levels of the essential oils in GC and HGC were 0.01% and 0.1%, respectively. There were 19 constituents of the essential oil of Gom-chewi: 14 carbohydrates, 4 alcohols, and 1 acetate, and the major constituents were L-β-pinene (36.02%), D-limonene (25.64%), α-pinene (24.85%) and β-phellandrene (5.39%). In the essential oil of HGC, 25 constituents were identified: 17 carbohydrates, 4 alcohols, 3 acetates, and 1 N-containing compound, and the major constituents of HGC were D-limonene (39.74%), L-β-pinene (35.43%) and α-pinene (11.94%). The minor constituents of HGC were ρ-cymene, γ-muurolene, γ-cadinene, germacrene D, ingol 12-acetate and butyl 9,12,15-octadecatriene and nimorazole were not identified in the GC essential oil. Overall, the results showed that the fragrance and chemical compositions of essential oils in GC and HGC differed, suggesting that both essential oils could be used for the development of perfumery products.

임상검체에서 Escherichia spp.의 분리 빈도와 항균제감수성

신현성(Hyun-Sung Shin),박연보(Youn-Bo Park),조경진(Kyung-Jin Cho) 고려대학교 보건과학연구소 2007 보건과학논집 Vol.33 No.1

The 4,579 Escherichia spp. were isolated from total 121,294 clinical materials from Dec 1, 2004 to Nov 30, 2006 in “C” hospital. Isolation frequency of Escherichia spp were 99.7% in E. coli, 0.2% E. hermannii and 0.1% E. fergusonii. %. Escherichia spp. were more frequently isolated in over 50 age group(8.7%-20.7%) than the below 50 age group(2.1-10.4%). According to the specimen types, 59.7% of the isolated Escherichia spp. were from urine (random 45.9% and catheterized urine 13.8%). In the susceptibility analysis of the Escherichia spp., using VITEK Ⅱ AST cards, 25.3%(536/2121) were extended spectrum β-lactamase(ESBL) E. coli. The antimicrobial agents that showed over 90% susceptibility were imipenem, amikacin, piperacillin/tazobactam and nitrofurantoin.

응급검사 소요시간의 요인 분석과 단축방안

오연숙,임재설,조현미,박연보,나기수,남광호,이복자,구선회,권계철 한국의료QA학회 2005 가을학술대회 Vol.2005 No.2

임상화학 검사의 Delta Check System 개발 및 정도관리 효율성 검토

권계철,임춘화,김문희,박연보 충남대학교 의과대학 지역사회의학연구소 2001 충남의대잡지 Vol.28 No.1

Background : The application of the delta check in tests in general chemistry detects both random and clerical errors, thus enhancing the reliability of tests. However, since this approach adds the burden of confirming the results to the laboratory, it has the disadvantage of lengthening the turn around time of tests. We speculated that an online delta check system needed to reduce the turn around time. We developed delta quality control system and evaluated its effectiveness of quality control in clinical chemistry. Methods : We developed an online delta check system based on the client-server paradigm. We used IBM PentiumⅢ PC as server and as clients. The database system used was powerbuilder 6.0. Results : In the system we developed, delta screening was performed when test results were input to the computer. The decision over the delta screening was made by comparing it against patient's clinical information and cumulative results within the same screen. Conclusions : The developed delta check method made it available to reduce the turn around time previously spent on delta screening by eliminating the batch processing of tests which was needed in previous approaches separate query cumulative results and patient's clinical informations for screening purposes.

가막사리(Bidens tripartita L.) 정유의 향취, 화학성분 및 세포독성

윤미선(Mi Sun Yun),연보람(Bo-Ram Yeon),조해미(Hae Me Cho),이사은(Sa Eun Lee),주진우(Jin-Woo Jhoo),정지욱(Ji Wook Jung),박유화(Yu-Hwa Park),김성문(Songmun Kim) 韓國雜草學會 2012 Weed&Turfgrass Science Vol.32 No.3

가막사리는 우리나라 전역의 논에서 다발생하여 농업에 막대한 피해를 주는 일년생잡초이지만 이를 활용하는 방안에 대해서는 연구된 바가 없다. 본 연구의 목적은 가막사리 정유를 향장산업제품의 소재로 활용할 수 있는지를 구명하는데 있다. 가막사리에 함유된 정유의 함량은 $0.028{\pm}0.003%$이었으며, 역치값은 0.1%이었으며, 향취는 green, herbal, oily, spicy이었다. 가막사리 정유에는 탄화수소 17종, 알코올 6종, 아세테이트 6종, 질소화합물 5종, 에테르 3종, 케톤 3종, 락톤 1종, 황화합물 1종 등 총 42종의 화학성분이 함유되어 있었으며, 다함량 성분은 α-phellandrene(22.50%), α-pinene(22.21%), 2,4-dimethyl(2,5-dimethylphenyl)methyl ester benzoic acid(15.11%), limonene(10.66%), β-pinene(35.43%), β-cubebene(5.27%)이었다. 가막사리 정유가 HaCaT 각질형성세포에 미치는 세포독성을 조사하기 위한 MTT assay를 실시한 결과, 가막사리 정유의 IC50값은 0.018%이었으며, 피부에 미치는 영향을 조사하기 위하여 피부자극유발시험을 수행한 결과 가막사리 정유 0.1% 처리에도 그 어떠한 발진이나 수포도 발견되지 않았다. 본 연구의 결과는 가막사리 정유가 향장제품을 위한 소재로 활용될 수 있다는 점을 시사하여 준다. The essential oil was extracted by steam distillation from the aerial part of erect bur-marigold (Bidens tripartita L.), one of the noxious weed in paddy field. The composition of the essential oil was analyzed by gas chromatography-mass spectrometry. The fragrance of the essential oil was green, herbal, oily, spicy. There were 42 constituents in the essential oil:17 hydrocarbons, 6 alcohols, 6 acetates, 5 N-containing compounds, 3 ethers, 3 ketones, 1 lactone and 1 S-containing compound. Major constituents were α-phellandrene (22.50%), α-pinene (22.21%), 2,4-dimethyl (2,5-dimethylphenyl) methyl ester benzoic acid (15.11%), limonene (10.66%), β-pinene (35.43%), and β-cubebene (5.27%). The IC50 value in MTT assay using HaCaT keratinocyte cell line was 0.018%. However, attachment of patch with 0.1% of the erect bur-marigold essential oil for 24 hr did not show any skin toxicity. Overall results of this study suggest that the essential oil of erect bur-marigold could be used as a source for the development of perfumery industrial products.

임상검체에서 Pseudomonas spp.의 분리빈도와 항균제 감수성

신현성 ( Hyun Sung Shin ),박연보 ( Youn Bo Park ),조경진 ( Kyung Jin Cho ) 대한임상검사과학회 2007 대한임상검사과학회지(KJCLS) Vol.39 No.3

From the total 121,294 clinical materials submitted to the Department of Laboratory Medicine of "C" hospital from December 1, 2004 to November 30, 2006, 3,408 Pseudomonas spp. were isolated. The isolation frequencies of Pseudomonas spp. were as follows, P. aeruginosa 95.5%, P. putida 2.5%, P. fluorescens 0.8%, along with low frequencies of P. luteola, P. alcaligenes, P. stutzeri, P. oryzihabitants, P. mendocina and unidentified Pseudomonas species. The isolation rates of Pseudomonas spp. according to season and sex were evenly distributed. The isolated frequency of Pseudomonas spp. in male was two times higher than that of in female showing significantly more male patients in surgical areas and more female patients in internal areas (p<0.001). In monthly analysis, Pseudomonas spp. were the most frequently isolated in July (10.4%), but lowest in February (5.6%). Half of Pseudomonas spp. were isolated from sputum (48.2%). In the susceptibility analysis of Pseudomonas spp. by VITEKⅡ AST cards, the Pseudomonas spp showing higher susceptibility against antimicrobial agents were piperacillin/tazobactam (82.7%) in P. aeruginosa; amikacin (84.7%), colistin (83.3%) in P. putida; and amikacin (96.3%), cefepime (87.5%), ceftazidime (87.5%) ciprofloxacin (92.3%), colistin (88.5%) gentamicin (96.2%), isepamicin (96,1%), meropenem (92.3%), netilmicin (96.0%), piperacillin/ tazobactam (95.4%) and tobramycin (92.6%) in P. fluorescens.

임상검체에서 Serratia spp.의 분리빈도와 항균제 감수성

신현성(Hyun-Sung Shin),박연보(Youn-Bo Park),조경진(Kyung-Jin Cho) 고려대학교 보건과학연구소 2007 보건과학논집 Vol.33 No.2

  Background : The most common hospital infections are of the urinary tract and respiratory diseases which covers three quarters of all the Serratia infections. That kind of nosocomial infections are from the urinary catheter or the instruments in the respiratory intensive care unit. Moreover the Serratia infection is closely related with the antibiotic resistance. The information on the antibiotic resistance against the Serratia spp. isolated from the patients admitted in the Korean medical institutions would be helpful to the building up a database for the antibiotic resistance of Serratia spp.<BR>  Methods : A medical records on the total of 509 Serratia spp. isolated from 116,429 clinical specimens requested to the Depratment of Laboratory Medicine of “C” hospital during the period from Jan. 1, 2005 to Dec.. 31, 2006 were investigated retrospectively and analyzed. In the test for Serratia spp. isolation, the specimens were cultivated in the condition of 37℃ incubator for two or three days after inoculation in Nutrient agar and blood agar plate, then pure culture bacteria were isolated. The bacteria were analyzed by the VITEK Ⅱ. VITEK Ⅱ-GN cards and VITEK Ⅱ system AST-N041 cards respectively for identification and antimicrobial susceptibility patterns, and CLSI M7 A7-MIC was applied for performance standards for antimicrobial susceptibility testing.<BR>  Results : The isolation frequencies of Serratia spp. were as follow, Serratia marcescens 94.7%, Serratia liquefaciens 4.1%, Serratia fonticola 0.4%, along with low frequencies in Serratia ficaria, Serratia odorifera, Serratia plymuthica and Serratia rubidaea. Seven strain Serratia spp. were identified with an isolation range of 0.2%-94.7%. The clinical materials showing over 5.0% isolation rate were 59.5% in sputum, 13.7% in urine, 5.7% in blood and 5.1% in wound against Serratia spp.. The antimicrobial agents that showed over 90.0% susceptibility were amikacin, cefepime, imipenenm, meropenem in Serratia marcescens, and piperacillin, tetracycline, amikacin, cefepime, cefotaxime, ceftazidime, gentamicin, ciprofloxacin, netilmicin, nitro furantoin, piperacillin+tazobactam, ofloxacin, ticarcillin, ticarcillin/clav.ac, and tobramycin in Serratia liquefaciens.<BR>  Conclusion : Severn species of genus Serratia were isolated from the requested clinical materials. The antimicrobial agents showing over 90% susceptibility against the whole groups of Serratia spp.(S. marcescens +S. liquefaciens) were 100.0% in meopenem, 99.6% in imipenem, 95.1% in cefepime, and 95.0% in amikacin. Among the applied 22 antibiotics, four antibiotics showed ?90% susceptibility against S. marcescencs(18.2%), while fifteen showed ?75% susceptibility against S. liquefaciens.<BR>  The increased number of resistant strains and ESBL producing Serratia spp. means that the number of available antimicrobial agents are becoming limited. Therefore, selecting effective antibiotics seems through the susceptibility test seems to be inevitable.

임상검체에서 분리된 사슬알균종의 분리빈도와 항균제 감수성

신현성 ( Hyun Sung Shin ),박연보 ( Youn Bo Park ) 대한임상검사과학회 2008 대한임상검사과학회지(KJCLS) Vol.40 No.1

From the total 116,429 clinical specimens submitted to “C” hospital from January 2005 to December 2006, 2,195 strains of streptococci were isolated. Twenty four species of Streptococcus were identified with 0.1~19.8% isolation frequencies, of which S. pneumoniae was 19.8%, S. agalactiae 16.2%, S. anginosus 9.8%, S. constellatus 5.0%, S. oralis 3.9%, S. mitis 3.3%, S. pyogenes 2.7%, S. salivarius subsp. salivarius 2.2%, S. sanguinis 1.9%. For S. pneumoniae, clinical specimens showing over 9.0% isolation rate were 82.8% in sputum, 9.2% in blood, and for Streptococcus species other than S. pneumoniae, 18.0% in sputum, 16.0% in urine and 9.7% in blood. The antimicrobial agents that showed over 90.0% susceptibility were cefotaxime, gatilfloxacin, imipenem, levofloxacin, linezolid, moxifloxacin, rifampin and sporfloxacin in S. pneumoniae, ampicillin, cefotaxime, cetriaxone, levofloxacin, linezolid, penicillin, quinupristin/dalfopristin and vancomycin in S. agalactiae, chloramphenicol, clindamycin, levofloxacin and vancomycin in S. anginosus, levofloxacin, vancomycin in S. constellatus subsp. constellatus, vancomycin in S. oralis, vancomycin in S. mitis, chloramphenicol, clindamycin, levofloxacin, quinupristin/dalfopristin and vancomycin in S. pyogenes, chloramphenicol, levofloxacin and vancomycin in S. salivarius subsp. salivarius, chloramphenicol, levofloxacin and vancomycin in S. bovis Ⅱ, chloramphenicol, levofloxacin, quinupristin/dalfopristin and vancomycin in S. dysgalactiae subsp. dysgalactiae, levofloxacin, chloramphenicol and vancomycin in the whole group of 10 Streptococcus spp. other than Streptococcus pneumoniae.