양파김치 메탄올추출물의 항암 및 면역활성

박경욱(Kyung-Uk Park),김재용(Jae-Yong Kim),조영숙(Young-Sook Cho),이성태(Sung-Tae Yee),정창호(Chang-Ho Jeong),강갑석(Kap-Suk Kang),서권일(Kwon-Il Seo) 한국식품영양과학회 2004 한국식품영양과학회지 Vol.33 No.9

양파 및 양파김치에 대한 항암 및 면역활성을 조사한 결과는 다음과 같다. 양파 및 양파김치 메탄올 추출물은 aflatoxin B₁로 돌연변이를 유도한 Salmonella typhimurium에 대하여 농도 의존적으로 항돌연변이 효과가 큰 것으로 나타났으며, 양파김치 첨가군이 양파의 첨가군보다 그 효과가 더욱 크게 나타났다. 양파 및 양파김치 메탄올 추출물은 A549 및 MCF-7 암세포주에 처리한 결과 추출물을 처리하지 않은 대조구에 비하여 1,000 ㎍/mL 농도에서 모두 20% 이상 그 성장을 억제하였다. 양파김치 메탄올 추출물은 농도에 비례하여 비장세포의 증식을 유도하였고, 양파 추출물보다 더 높은 증식을 유도하였다. 양파 및 양파김치 메탄올 추출물을 처리한 대식세포주에서 NO의 생성이 농도 의존적으로 증가하였으며, 역시 양파김치 추출물이 양파 추출물보다 더 많은 NO을 생성량을 유도하였다. Antitumor activities of onion methanol extract (OME) and onion Kimchi methanol extract (OKME) were investigated by using aflatoxin B₁-mediated Salmonella typhimurium mutagenicity and the model of cytotoxicity on the cancer cell lines. Their immune activities were also investigated by using mouse spleen cells and macrophage cell lines, respectively. OME and OKME showed the enhanced antimutagenicity in a dose-dependent manner in particular, the activity of OKME was higher than that of OME. OME and OKME decreased over 20% of the proliferation of the A549 (lung cancer cell) and MCF-7 (breast cancer cell) cell lines when compared with the control at 1,000 ㎍/mL. The proliferation of mouse spleen cells and the NO production in marcrophage cell lines treated OME and OKME were increased in a dose-dependent manner compared with untreated control cells, and their activities of OKME were higher than those of OME.

대두박 식용사포닌의 항산화 및 대장암세포 성장 억제효과

박경욱 ( Kyung Uk Park ),김재용 ( Jae Yong Kim ),서권일 ( Kwon Il Seo ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2009 한국식품저장유통학회지 Vol.16 No.5

변산반도국립공원 내 이식된 미선나무군락지의 식생분석 및 생육특성

박경욱(Kyung-Uk Park),오현경(Hyun-Kyung Oh),김영하(Young-Ha Kim),양주영(Ju-Yung Yang) 국립공원연구원 2010 국립공원연구지 Vol.1 No.4

전라북도 임실군 병아리꽃나무 자생지의 생태적 특성

박경욱 ( Kyung Uk Park ),변무섭 ( Mu Sup Beon ),오현경 ( Hyun Kyung Oh ),유주한 ( Ju Han You ) 한국환경영향평가학회 2015 환경영향평가 Vol.24 No.4

The purpose of this study is to offer the basic data to the habitat conservation and management by surveying and analysing the ecological characteristics such as the flora, vegetation structure and soil of Rhodotypos scandens habitat. The flora were summarized as 131 taxa including 57 families, 99 genera, 107 species, 1 subspecies, 17 varieties and 6 forms. The life forms grouped as in the follows; the megaphanerophytes(MM) were 21 taxa, 24 taxa of the microphanerophytes(M), 30 taxa of nanophanerophytes(N), 6 taxa of chamaiphytes(Ch), 20 taxa of hemicryptophytes(H), 17 taxa of geophytes(G), 12 taxa of therophytes(Th) and 1 taxa of hydrophytes(HH). The present conditions of communities were 8 types including Zelkova serrata, Quercus aliena, Ulmus parvifolia, Rhamnella frangulioides, Castanea crenata, Albizia julibrissin, Celtis sinensis and Robinia pseudoacacia. In case of the dominant species by layers, the tree layer species were Zelkova serrata, Quercus aliena, Ulmus parvifolia, Castanea crenata, Albizia julibrissin, Celtis sinensis and Robinia pseudoacacia, and the subtree layer species were Rhamnella frangulioides, Q. aliena, Z. serrata, A. julibrissin, U. parvifolia and Broussonetia kazinoki. The shrub layer species was Rhodotypos scandens. In the results of analyzing the diversity index, H’ was from 1.691 to 2.610, from 2.197 to 3.466 in H’max, from 0.646 to 0.903 in J’ and from 0.097 to 0.354 in D. In the results of analysing the soil, there showed that acidity was 5.6, 0.5dS/m of electrical conductivity(EC), 59.1mg/kg of available P2O5, 49.7% of organic matter content, 0.4cmol+/kg of exchangeable K+, 13.5cmol+/kg of exchangeable Ca2+, 3.3cmol+/kg of exchangeable Mg2+.

대두박으로부터 분리한 식용 조사포닌의 항암 및 면역활성

박경욱(Kyung-Uk Park),위재준(Jae-Joon Wee),김재용(Jae-Yong Kim),정창호(Chang-Ho Jeong),강갑석(Kap-Suck Kang),조영숙(Young-Sook Cho),서권일(Kwon-Il Seo) 한국식품영양과학회 2005 한국식품영양과학회지 Vol.34 No.10

새로운 기능성 식품 소재를 개발하기 위하여 대두박으로부터 HP-20 수지를 이용하여 식용조사포닌을 분리한 후 이들에 대한 항암 및 면역 활성을 조사하였다. 분리한 식용사포닌은 1,000 ㎍/mL의 농도에서 A-549, MCF-7 및 SW480과 같은 암세포주의 성장을 강하게 억제하였으며, 폐암세포주에 1000 ㎍/mL의 농도를 사포닌을 첨가였을 때 세포의 형태가 심하게 변화되었다. 사포닌은 생쥐의 비장으로부터 분리한 면역세포에 대하여 1 ㎍/mL의 농도까지는 대조구에 비하여 그 증식을 유도하였으나 그 농도이상에서는 오히려 감소하는 경향을 보였다. 또한 대식세포주인 RAW 264.7에 사포닌의 처리 시 농도 의존적으로 NO의 함량이 높게 생성되었다. To develop a new functional food material, edible crude saponin was isolated from soybean cake using HP-20 resin and its anticancer effect and immuno-activity were investigated. The saponin significantly inhibited the growth of cancer cells such as A549, MCF-7 and SW480 at a concentration of 1,000 ㎍/mL. Morphological changes was observed in the A549 cells surface treated with the saponin of 1,000 ㎍/mL concentration. The proliferation of mouse spleen cells treated with saponin was increased in a dose-dependent manner compared with untreated control cells until the concentration of 1 ㎍/mL but decreased at higher concentrations than that. The NO production in marcrophage cell lines (RAW 264.7) treated with saponin was increased in a dose- dependent manner compared with untreated control cells.

홍경천 추출물의 생리활성

박경욱 ( Kyung Uk Park ),윤재호 ( Jae Ho Yoon ),김재용 ( Jae Yong Kim ),정창호 ( Chang Ho Jeong ),박채규 ( Chae Kyu Park ),송원섭 ( Won Seob Song ),서권일 ( Kwon Il Seo ) 한국식품저장유통학회 ( 구 한국농산물저장유통학회 ) 2005 한국식품저장유통학회지 Vol.12 No.5

변산반도국립공원 내 이식된 미선나무군락지의 식생분석 및 생육특성

오현경(Hyun-Kyung Oh),김영하(Young-Ha Kim),양주영(Ju-Yung Yang),박경욱(Kyung-Uk Park) 국립공원연구원 2010 국립공원연구지 Vol.1 No.3

본 연구는 부안댐 건설로 인해 수몰위기에 처해있는 미선나무 개체를 이식한 지역으로 현재 변산반도국립공원 특별보호구 내에 위치하고 있어 일반인의 접근이 불가능하며, 부안군 변산면 중계리 청림에 있는 천연기념물(제370호) 군락지보다 개체수가 많고 생육상태도 매우 양호하여 식생분석 및 생육특성을 조사한 결과, 다음과 같다. 변산반도국립공원 내 이식된 미선나무군락지의 식물상은 21과 33속으로 총 35종류가 확인되었다. 이중 미선나무는 환경부의 법정보호종인 멸종위기야생식물 II급, IUCN 평가기준에 따른 희귀식물 중 멸종위기종 및 우리나라 특산식물이다. 미선나무군락지 내의 군집구조는 2개의 조사구를 분석하여 자귀나무-졸참나무군락, 자귀나무군락으로 구분되었다. 이중 층위별 중요치는 자귀나무-졸참나무군락에서 팽나무, 비목, 신나무 순으로, 자귀나무군락에서 산뽕나무, 신나무, 팽나무 순으로 분석되었다. 미선나무의 중요치는 자귀나무-졸참나무군락에서 3.7%, 자귀나무군락에서 6.0% 로 분석되었다. 미선나무 개체군이나 잎의 형태적 특성 또한 2개의 조사구에서 채취하거나 분석하였다. 이중개체군은 조사구 1번에서 43개체, 조사구 2번에서 34개체를 정밀 분석하였으며, 미선나무 잎 60개를 채취하여 형태적 특성을 분석한 결과, 평균 엽장은 73.0 mm, 평균 엽폭은 35.0 mm, 평균 엽병장은 4.9 mm 이었다. This study investigated the area which Abeliophyllum distichum in danger of being submerged because of Buan-dam construction were replanted to analyze vegetation and growing condition. This area is located in specially preserved area of Byeonsanbando National Park, so this area is not open to the public. Abeliophyllum distichum growing in this area are growing more than a community of Abeliophyllum distichum located in Cheongrim, Jungkye-ri, Byeonsan-myeon, Buan-gun and designated as a natural monument (No. 370) and growing conditions for Abeliophyllum distichum are also better. As a result of study; we found that there are 21 families, 33 genus, 35 taxa and among these species the Abeliophyllum distichum are legally preserved species (Grade II) from Ministry of Environment, endangered species of rare species from standards of IUCN standards and also one of endemic plants in Republic of Korea. The structure of this community is classified as community of Albizzia julibrissin-Quercus serrata and community of Albizzia julibrissin from investigating 2 enumeration districts. The hierarchy of importance value is in the order of Celtis sinensis, Lindera erythrocarpa and Acer ginnala in the community of Albizzia julibrissin-Quercus serrata and in the order of Morus bombycis, Acer ginnala and Celtis sinensis in the community of Albizzia julibrissin. The percent of importance value of Abeliophyllum distichum is 3.7% in the community of Albizzia julibrissin-Quercus serrata and 6.0% in the community of Albizzia julibrissin. Moreover, in order to analyze characteristics of population of Abeliophyllum distichum and leaves shape, 43 individuals from first district, 34 individuals from second district and 60 leaves of Abeliophyllum distichum are closely examined. As a result, the average leaf length is 73.0 mm, the average leaf width is 35.0 mm and the average petiole length is 4.9 mm.

변산반도 노랑붓꽃과 붉노랑상사화 자생지의 식물상 및 식생현황

오현경 ( Hyun Kyung Oh ),한윤희 ( Yun Hee Han ),박경욱 ( Kyung Uk Park ) 전북대학교 농업과학기술연구소 2011 농업생명과학연구 Vol.42 No.2

The vascular plants in habitats Lycoris flavescens and Iris koreana, Byeonsanbando were listed 147 taxa; 54 families, 106 genera, 117 species, 3 subspecies, 24 varieties and 3 forms. 10 spots; extremely much plants of 59 species (No. 6) and extremely few plants of 16 species (No. 7). Divided into 147 taxa; woody plants were 76 taxa (51.7%) and herbaceous plants were 71 taxa (48.3%). Based on the list of legal protection species by the Ministry of Environment, 2 taxa; Abeliophyllum distichum, Iris koreana. Based on the list of rare plants by the Korea Forest Service and Korea National Arboretum, 5 taxa; Asarum maculatum and Eranthis byunsanensis (Least Concerned), Abeliophyllum distichum and Iris koreana (Critically Endangered), Iris minutiaurea (Vulnerable) and Korean endemic plants, 7 taxa (Philadelphus schrenkii var. schrenkii, Weigela subsessilis, Lycoris flavescens, etc.). Based on the specific plants by floral region were total 25 taxa (17.0% of all 147 taxa of vascular plants); Abeliophyllum distichum in class Ⅴ, Iris koreana in class Ⅲ, 4 taxa (Acer triflorum, Bupleurum longiradiatum, Cymopterus melanotilingia, etc.) in class Ⅱ. 19 taxa (Quercus variabilis, Clematis patens, Anemone raddeana, etc) in class Ⅰ. Especially, the part of habitats of Lycoris flavescens and Iris koreana which has a distribution around most of habitates have a wide distribution around valley so that degraded flood plain will be concerned because of the heavy rainfall and consequentially, a long term of monitoring is suggested for artificially estimating disturbances.

오봉산-국사봉 구간의 관속식물 분포 특성

오현경 ( Hyun Kyung Oh ),한윤희 ( Yun Hee Han ),박경욱 ( Kyung Uk Park ),변무섭 ( Mu Sup Beon ) 전북대학교 농업과학기술연구소 2011 농업생명과학연구 Vol.42 No.1

The vascular plants in Obongsan-Kuksabong sector were listed 249 taxa; 76 families, 167 genera, 212 species, 1 subspecies, 31 varieties and 5 forms. Divided into 249 taxa; woody plants were 111 taxa (44.6%) and herbaceous plants were 138 taxa (55.4%). Based on the list of naturalized plants, 7 taxa (Phytolacca americana, Trifolium repens, Oenothera odorata, Erechtites hieracifolia, etc.) and naturalization rate was 2.8% of all 249 taxa of vascular plants. Based on the list of rare plants by the Korea Forest Service and Korea National Arboretum, 3 taxa; Chionanthus retusa (Least concerned), Tricyrtis dilatata (Least concerned), Lilium callosum (Vulnerable) and Korean endemic plants, 5 taxa (Philadelphus schrenckii, Indigofera koreana, Weigela subsessilis, etc.). Based on the specific plants by floral region were total 17 taxa (6.8% of all 249 taxa of vascular plants); Weigela florida in class Ⅱ, 16 taxa (Quercus variabilis, Ulmus parvifolia, Spiraea blumei, Orixa japonica, Ilex macropoda, Grewia biloba var. parviflora, Vaccinium oldhami, Weigela florida, Veratrum versicolor, etc.) in class Ⅰ. The communities of vegetation present in Obongsan-Kuksabong sector separated from 2 sites of Pinus densiflora community, Platycarya strobilacea community and 6 sites of Quercus variabilis community.

변산반도국립공원 변산바람꽃 자생지내의 관속식물상 및 식생현황

오현경(Hyun-Kyung Oh),박경욱(Kyung-Uk Park),소민석(Min-Seok Soh),이지희(Ji-Hee Lee) 국립공원연구원 2011 국립공원연구지 Vol.2 No.2

변산바람꽃 자생지내의 관속식물상은 60과 112속 145종 15변종 2품종으로 총 162분류군이 확인되었다. 이중 목본식물은 79분류군(48.8%), 초본식물은 83분류군(51.2%)이 확인되었다. 관속식물상 중 법정보호종은 미선나무와 노랑붓꽃, 희귀식물은 개족도리, 변산바람꽃 등 4분류군, 특산식물은 진범, 고광나무, 병꽃나무, 청괴불나무, 붉노랑상사화 등 9분류군이 확인되었다. 또한 식물구계학적 특정식물은 V등급에 미선나무, III등급에 단풍나무, 이나무 등 4분류군, II등급에 복자기, 개시호, 왕쌀새 등 3분류군, I등급에 굴참나무, 꿩의바람꽃, 왜현호색, 회양목, 상산, 합다리나무, 장구밥나무, 오갈피, 정금나무, 올괴불나무, 큰천남성 등 18분류군으로 총 26분류군(전체 162분류군의 관속식물 중 16.0%에 해당)이 확인되었다. 특이사항으로는 변산바람꽃 자생지 일부가 풍화토인 전석지에 위치하여 토양침식이나 여름철 집중호우로 인한 훼손이 우려되고 탐방로 주변에 있어 인위적인 교란 가능성이 높기 때문에 향후 장기적인 모니터링이 요구된다. The vascular plants of habitats Eranthis byunsanensis were listed 162 taxa; 60 families, 112 genera, 145 species, 15 varieties and 2 forms. Divided into 162 taxa; woody plants were 79 taxa (48.8%) and herbaceous plants were 83 taxa (51.2%). Based on the legal protection species, endangered wild plants II were recorded; Abeliophyllum distichum, Iris koreana and rare plants by the Korea Forest Service and Korea National Arboretum, 4 taxa; Asarum maculatum, Eranthis byunsanensis and endemic plants, 9 taxa; Aconitum pseudolaeve var. erectum, Philoadelphus schrenckii, Weigela subsessilis, Lonicera subsessilis, Lycoris flavescens. Based on the specific plants by floral region were total 26 taxa (16.0% of all 162 taxa of vascular plants); Abeliophyllum distichum in class V, 4 taxa (Acer palmatum, Idesia polycarpa, etc.) in class III, 3 taxa (Acer triflorum, Bupleurum longiradiatum, Melica nutans) in class II, 18 taxa (Quercus variabilis, Anemone raddeana, Corydalis ambigua, Buxus microphylla var. koreana, Orixa japonica, Meliosma oldhamii, Grewia biloba var. parviflora, Acanthopanax sessiliflorus, Vaccinium oldhami, Lonicera praeflorens, Arisaema ringens, etc.) in class I. Especially, the part of habitats of Eranthis byunsanensis which has a distribution around weathering soil concerns about a estimating damages due to future landslip, most of habitates have a wide distribution around valley so that degraded flood plain will be concerned because of the heavy rainfall and consequentially, a long term of monitoring is suggested for artificially estimating disturbances.