배에서 추출된 Pectin과 Phenolic compound가 흰쥐의 국소뇌혈류 및 심혈관계에 미치는 영향

나창수 ( Chang Su Na ),윤대환 ( Dae Hwan Youn ),최동희 ( Dong Hee Choi ),정종길 ( Jong Gil Jeong ),은종방 ( Jong Bang Eun ),김정상 ( Jeong Sang Kim ) 대한본초학회 2003 大韓本草學會誌 Vol.18 No.2

N/A Objectives : Oriental pear was used as treating disorder of heart and blood circulation in oriental medicine. This study was designed to investigate that pear pectin, pear phenolic compound effects on cardiovascular system as regional cerebral blood flow(rCBF), mean arterial blood pressure(MAP), heart rate, cardiac contractile force. Methods : The experiments were performed on Sprague Dawley rats. 2K1C hypertension model was prepared by constricting the left renal artery with a sliver clip. Apple pectin, pear pectin and pear phenolic compound solutions were administrated via femoral vein for Sweeks, a day`s interval. Animals were divided into four groups, administration of apple pectin(AP1, pear pectin(PP) and pear phenolic compound( PPC ). Results : The results are that KBF was significantly increased at lmglkg in PPC group. The MAP was significantly decreased at l0㎍/kg in apple pectin, at l0㎍/kg, 100㎍/kg in pear pecitn than before adminstration, but increased at 1 m p d k g in pear phenolic compound. There was no significant effects in heart rate. Cardiac contractile force was significantly increased a at l0㎍/kg, l00㎍/kg, lmg/kg in pear phenolic compound. Conclusions : These results suggests that pear phenolic compound and pear pectin may be a significant effect in symptoms of heart and blood circulation..

합곡(合谷) 복유(復溜) 보사(補瀉) 시술(施術)이 소한(小汗)에 미치는 영향(影響)(2)

나창수 ( Chang Su Na ),최찬헌 ( Chan Hun Choi ),김영선 ( Young Sun Kim ),김왕인 ( Wang In Kim ),윤대환 ( Dae Hwan Youn ) 대한경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.2

Objectives: The purpose of this study is to identify the effect of tonifying or sedative manipulation on LI4 and KI7 for sweet deficiency under cold environment. Methods: The participants were divided into 4 groups; intact group without acupuncture(Intact), acupuncture group inserting straightly with LI4 & KI7 method((LI4-straight·KI7-straight), acupuncture group inserting·twisting with LI4 tonificaton & KI7 tonificaton method(LI4-tonify·KI7-tonify) and acupuncture group inserting·twisting with LI4 sedation & KI7 sedation method(LI4-purge·KI7-purge). We obtained the baseline data under cold condition with the temperature 14±1℃. After making the participant staying under cold condition for 10 minute, we measured sweating rate on skin surface and biological responses such as body temperature, oxygen saturation, pulse rate, systolic blood pressure, diastolic blood pressure, deoxy-Hb and oxy-Hb in subcutaneous. Results: The sweating rate on skin surface was not different in all treatment groups compared to the intact group. The body temperature was significantly increased in LI4-straight·KI7-straight group compared to the intact group. The oxygen saturation significantly increased in LI4-purge·KI7-purge group compared to the intact group. The systolic blood pressure was significantly increased in LI4-purge·KI7-purge group compared to the intact group. The deoxy-Hb was significantly decreased in LI4-purge·KI7-purge group compared to the intact group. Conclusions: The above results, the effect of LI4, KI7 acupuncture due to the tonifying or sedative manipulation on sweating control could be observed. However, other biological responses with the acupuncture of tonifying or sedative manipulation were giving different expressions. We suggest that continued research of tonifying or sedative manipulation is of importance order to find these expressions.

합곡(合谷) 복류(復溜) 보사(補瀉) 시술(施術)이 소한(少汗)에 미치는 영향(影響)(II)

나창수,최찬헌,김영선,김왕인,윤대환,Na, Chang-Su,Choi, Chan-Hun,Kim, Young-Sun,Kim, Wang-In,Youn, Dae-Hwan 경락경혈학회 2010 Korean Journal of Acupuncture Vol.27 No.2

Objectives : The purpose of this study is to identify the effect of tonifying or sedative manipulation on LI4 and KI7 for sweet deficiency under cold environment. Methods : The participants were divided into 4 groups; intact group without acupuncture(Intact), acupuncture group inserting straightly with LI4 & KI7 method((LI4-straight KI7-straight), acupuncture group inserting twisting with LI4 tonificaton & KI7 tonificaton method(LI4-tonify KI7-tonify) and acupuncture group inserting twisting with LI4 sedation & KI7 sedation method(LI4-purge KI7-purge). We obtained the baseline data under cold condition with the temperature $14{\pm}1^{\circ}C$. After making the participant staying under cold condition for 10 minute, we measured sweating rate on skin surface and biological responses such as body temperature, oxygen saturation, pulse rate, systolic blood pressure, diastolic blood pressure, deoxy-Hb and oxy-Hb in subcutaneous. Results : The sweating rate on skin surface was not different in all treatment groups compared to the intact group. The body temperature was significantly increased in LI4-straight KI7-straight group compared to the intact group. The oxygen saturation significantly increased in LI4-purge KI7-purge group compared to the intact group. The systolic blood pressure was significantly increased in LI4-purge KI7-purge group compared to the intact group. The deoxy-Hb was significantly decreased in LI4-purge KI7-purge group compared to the intact group. Conclusions : The above results, the effect of LI4, KI7 acupuncture due to the tonifying or sedative manipulation on sweating control could be observed. However, other biological responses with the acupuncture of tonifying or sedative manipulation were giving different expressions. We suggest that continued research of tonifying or sedative manipulation is of importance order to find these expressions.

신문(神門), 태백혈(太白穴)에 시행된 직자법(直刺法), 수기사법(手技瀉法)및 침습형 레이저 시술이 고혈압 백서에 미치는 영향

나창수 ( Chang Su Na ),윤대환 ( Dae Hwan Youn ),최찬헌 ( Chan Hun Choi ),이석희 ( Suk Hee Lee ),오광환 ( Kwang Hwan Oh ),정성호 ( Sung Ho Jeong ) 대한경락경혈학회 2008 Korean Journal of Acupuncture Vol.25 No.2

Objectives: This study was performed to investigate the effect of invasive Laser acupuncture therapy with green Light (LAT-G) at HT7(Sinmun), SP3(Taebaek) on the blood pressure in hypertensive rat induced by two kidney one clip. Methods: The experiments were performed on Sprague Dawley rats. 2K1C hypertension model was prepared by constricting the Left renal artery with a sliver clip. Animals were divided into four groups, which were simple acupuncture treatment group with straight needle insertion on the square(AT-SS), acupuncture treatment group with reducing manipulation in the opposite channel direction(AT-RD), Laser acupuncture treatment group with green Light 532 nm, 10mW power in the opposite channel direction(LAT-G10) and Laser acupuncture treatment group with green Light 532 nm, 20mW power in the opposite channel direction(LAT-G20). The treatments were performed once per two days for 10 days. Results: Body weight was increased significantly in LAT-G20 group compared with AT-RD group. The blood pressure was significantly decreased in LAT-G20 and LAT-G10 groups compared with AT-SS group. Conclusions: These results suggest that green Laser acupuncture therapy at SP3·HT7 is more effective than straight needle insertion on the square for controlling hypertension. It is possible that invasive green Laser acupuncture therapy (532 nm) can be used as a reducing method of the traditional acupuncture techniques.

모혈(募穴)의 탄력(彈力) 상태(狀態) 측정(測定)에 의한 허실(虛實) 진단(診斷) 연구(硏究)

나창수,윤여충,박현철,이동규,최찬헌,장경선,소철호,Na, Chang-Su,Yoon, Yeo-Choong,Park, Hyun-Cheal,Lee, Dong-Kyu,Choi, Chan-Hern,Jang, Kyung-Sun,So, Cheal-Ho 대한침구의학회 2000 대한침구의학회지 Vol.17 No.1

The meridian system is the most essential and basic connecting structure that maintains the vital activities of viscera and bowels by connecting them with each part of body's surface. Doctors can understand the healthy condition, and the region and deficiency-excessiveness of disease by observing the condition of Qi flowing. Deficiency and excessiveness could be differentiated by various symptoms expressed in meridian system. Especially there could be several clues like pain, heat-cold, protuberance-depression, change of color and shine in the line of channel leads to the judgment of deficiency-excessiveness The diagnosis of deficiency and excessiveness can be generalized by quantification of elastic status in skin surface along the meridian system. By comparing data from measurement of elastic condition with those from traditional deficiency and excessiveness, it could be utilized for the development of oriental medicine. All biological activities in the human body are based on meridian system according to the oriental medicine. Also the meridian system is viewed as basic and essential structure connecting internal viscera and each part of body. The areas of expressed channel phenomena are muscle to bone, muscle to muscle and bone to bone. These areas are called depression where meridian system is present and any changing state on those points can be measured. It could be difficult in diagnosing the reaction of meridian system because doctor can depend on his own judgment. Therefore, it is necessary to quantify and indexate channel reactions. To quantify the channel reactions, specially manufactured instrument was used to quantify the protuberance and depression to differentiate the deficiency and excessiveness. The results follow as below; 1. The elastic index measurement by the equipment proved a pattern of agreement showing the values that ranged within standard deviation 0.05kgf/cm throughout the experiment except few cases' measurement in CV-17. 2. To evaluate the state of deficiency & excessiveness of elastic index measurements in frontal point, elastic index measurements in the front paint were compared to the elastic index measured surrounding the point within 2.5 cm. Such result of indexing procedure was closely matched to the concept of palpitation. 3. If the elastic index values in the surrounding front point closely located to the elastic index values in the front point, the judgement on the state of deficiency and excessiveness was delayed. Otherwise, it was judged as deficiency or excessiveness. 4. Out of total 12 cases of comparing the elastic index values to the elastic index values in the surrounding front point, Three to nine front points were judged as either in the state of deficiency or excessiveness. 5. Among the nine front points judged as either in the state of deficiency or excessiveness, Four cases were matched to the electric index measured by EAV that evaluating the internal organs by five different phases. If more clinical cases are accumulated, it is expected to systematically theorize and improve the concept of deficiency and excessiveness in the internal organs using the front point.

부자(附子)오두(烏頭) 중독증(中毒症)에 관한 소고(小考)

나창수,윤여충,Na, Chang-Su,Yun, Yeo-Chung 대한한의학회 1995 대한한의학회지 Vol.16 No.1

In order to study on Bu-ja(Oh-du) toxicosis, the outhor investigated many documents. The results obtained were as follows: 1. Toxicosis of Bu-ja(Oh-du) arised mainly from carelessness in use. 2. In the symptoms of Bu-ja(Oh-du), Heart System(心系) symptom often came out. 3. The general and local paralysis, falling of blood pressure in Heart System(心系) symptom : vertigo, cramp in Liver System(肝系) symptom : nausea, vomiting in Spleen System (脾系) symptom : difficulty of breathing in Lung System(肺系) symptom : wasting disease in Kidney System (腎系) symptom frequently originated from toxicosis of Bu-ja(Oh-du). 4. In the detoification method, there are atropin injection, carrying out a gastric lavage, and supplying a liquid medicine from the western medicine : taking ginger-licorice root decoction, taking mung beans thick decoction and getting vomited with soaked cinnamon from the oriental medicine. And also keeping warm and oxygen inhalation were used to counteract poison. 5. A pregnant woman, heat of excess type(實熱), yang sydrome(陽證 )must not be taken Bu-ja(Oh-du).

고지혈증 유발 백서에서 열결 ${\cdot}$ 여구에 대한 침자 및 강도별 레이저조사가 체중, 식이효율, 지질대사 및 동맥경화지수에 미치는 영향

나창수,윤대환,조명래,강자돈,Na, Chang-Su,Youn, Dae-Hwan,Cho, Myung-Rae,Kang, Ja-Don 경락경혈학회 2007 대한침구의학회지 Vol.11 No.1

Objectives : This research was performed to investigate the effect of acupuncture using invasive low level laser therapy (LLLT) at Yolgyol (LU7) + Yogu (LR5) on weight gain, food intake, food efficiency, lipid metabolism, atherogenic index, HTR (HDL-cholesterol to total cholesterol ratio) and morphological change of hepatic tissue in hyperlipdemia rats. Methods : Experimental groups were divided into high fat diet group (Control group), high fat diet and acupuncture therapy group at LU7 + LR5 (AT group), high fat diet and acupuncture group using 10 mW LLLT at LU7 +LR5 (LA10 group), high fat diet and acupuncture group using 20 mW LLLT at LU7+LR5(LA20 group), high fat diet and acupuncture group using 60 mW LLLT at LU7 + LR5 (LA60 group), once per 3 days during 9 weeks. Results : Body weight was decreased significantly in AT and LA20 groups compared with Control group. Food intake was increased significantly in LA60 group compared with Control group. Food efficiency was decreased significantly in LA10, LA20 and LA60 groups compared with control group. In the lipid metabolism, total cholesterol was decreased significantly in AT, LA10, LA20 and LA60 groups, triglyceride was decreased significantly in LA10, LA20 and LA60 groups, TG/HDL-cholesterol ratio was decreased significantly in LA 60 group compared with control group. In the morphological change, hepatic tissue were not showed balloning degeneration and irregular arrangement of hepatic cell in LA10 and LA20 groups with control group. Conclusions : Acupuncture using LLLT at LU7+LR5 can manage hyperlipemia by controlling body weight, food intake, food efficiency ratio and lipid metabolism.

배(梨) 추출 Phenolic Compound 및 길경(桔梗) 행인(杏仁) 배합 투여가 Ovalbumin으로 유발된 천식 동물 모델에서 기관지 평활근 장력, 호산구 및 IL-4에 미치는 영향

나창수 ( Chang Su Na ),정종길 ( Jong Gil Jeong ),윤대환 ( Dae Hwan Youn ) 대한본초학회 2007 大韓本草學會誌 Vol.22 No.2

치매에 관한 최근의 연구 동향

나창수,김정상,채우석,박석천,Na, Chang-Su,Kim, Jeong-Sang,Chae, U-Seok,Park, Seok-Cheon 대한한방내과학회 1998 大韓韓方內科學會誌 Vol.19 No.1

Dementia is the neurodegenerative process that affects cognition, behavior and function and one of the most prominent diseases of dementia is Alzheimer's disease(AD). AD is a dementing illness characterized clinically by the progressive and irreversible deafferentation of the limbic system, association neocortex and basal forebrain. A number of conditions are known to be predisposing risk factors for AD. In several of these, initiation of glial-mediated inflammatory pathways as a mechanism of AD is getting a lot of attention. On the other hand, a biochemical marker for monitoring the onset and progression of the disease would be a valuable tool for disease management. Also such a marker might be used as an end point in clinical intervention protocols. This biochemical marker will have the potential for identifying subjects afflicted with the disease and possibly for monitoring the onset and longitudinal progression of the disease. Here we have reviewed the latest papers of different approaches to AD. Of course, there is a section of PET which is very useful clinically nowadays.

척택.어제 침자가 고해상도 적외선 카메라로 관찰한 촌구맥 부위의 온도 Pulsation 변화에 미치는 영향 연구

나창수,정효상,김지현,Na, Chang-Su,Jeon, Hyo-Sang,Kim, Jee-Hyun 대한의용생체공학회 2011 의공학회지 Vol.32 No.2

Arteria radialis is a branch of the brachial artery extending down the forearm around the wrist where it closes to skin surface. In the oriental medicine, the skin above arteria radialis has an important role because oriental medicine practitioners put their finger tips on the area, and diagnose patient's health conditions by feeling the pulsation of the arterial contraction. The finger tip diagnostic method relies on subjective decision of the practitioner; and there is a need to develop an objective diagnostic modality. The pulsation of the arterial contraction appears not only a movement on the site but also as temperature fluctuation due to pulsatile feeding of warmer blood. The goal of this study is to demonstrate a feasibility of using an infrared camera quantitatively to detect the temperature fluctuation on the skin. Clinical important three different areas, called chon, gwan, chuk, near a wrist where the arteria radialis reaches close to skin surface are marked with small pieces of surgical tape. A high-speed and high-resolution infrared camera with a 3 cm of field of view measures these areas for 10 second at 200 frames per second with a 320*240 pixel size. The pulsatile temperature fluctuation is calculated after passing a band pass filter to remove any stationary temperature over 10 second. The temperature fluctuation of a healthy male volunteer is measured at a room temperature as a control, and is compared with another measurement performed after 20 minutes staying in a room at a 40 degree Celsius. This comparison is repeated for three times, and indicates that the fluctuation increases after staying 20 minutes in the warm room. This increase becomes smaller when the person stays in the warm room with an acupuncture treatment that decreases body temperature. So that an objective diagnostics on the site may become feasible.