화장실 공기 중 미생물 분포 조사연구

김종규,김아혁,김중순,Kim, Jong-Gyu,Kim, A-Hyeok,Kim, Joong-Soon 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.4

Objectives: This study was performed to examine bioaerosols in indoor air in public restrooms, as well as to assess the effects of air temperature and relative humidity on bioaerosol levels. Methods: A cross-sectional survey was performed in ten male and ten female restrooms. An air sampler (Anderson type) was used for sampling total suspended bacteria (TSB), Gram-negative bacteria (GNB), Gram-positive bacteria (GPB), opportunistic bacteria (OP), Staphylococcus spp., and total suspended fungi (TSF). Results: The levels of TSB were $10-10^2CFU/m^3$ and TSF $10-10^2CFU/m^3$, respectively. The GNB level was $0-10CFU/m^3$, and GPB and OP levels were $10-10^2CFU/m^3$. Staphylococcus aureus and methicillin-resistant S. aureus (MRSA) were detected in 90% of the restrooms. The GPB level was higher in the female restrooms than in the male restrooms (p < 0.05). TSB, GNB, and TSF showed higher levels in restrooms in buildings over 30 years old (p < 0.01). The main effect of air temperature or relative humidity and interaction effect of the two factors on the TSB level were significant (p < 0.05), while the effect of relative humidity on the TSF level was significant (p < 0.001). Conclusions: These results indicate that there is a wide variation in the bioaerosol levels among different restrooms. The observed differences in bioaerosol levels reflect different building histories. The effects of air temperature and/or relative humidity reveal that bioaerosol levels may vary according to season or time of day. Future research is needed to further characterize the relation between the bioaerosol levels and surface contamination in restrooms.

Pellet Semen 의 Glycerol 첨가방법과 평형시간이 한우의 정액성상과 수태에 미치는 영향

김종규,서국성,신원집,이용빈 ( J . K . Kim,G . S . Suh,W . J . Shin,Y . B . Lee ) 한국축산학회 1973 한국축산학회지 Vol.15 No.2

This experiment was conducted to study the possibility of pellet semen as a replacement for ampoule semen and simplifying the method of glycerol addition to the egg yolk extender with Korean native cattle. Glycerol was added once and twice to the egg yolk semen extender and equilibration time was 6, 12 and 18 hrs. respectively. Each semen was freezed in the liquid nitrogen and thawed in a 5℃ water bath. Inseminated artificially frozen semen to the cows and calculated the fertility (N.R. 60-90 days) and sperm living index. The results obtained are as follows: 1. No significant difference was found between once and twice additions of glycerol on the living index of spermatozoa. 2. No significant difference was found between, 6, 12 and 18 hours equilibration intervals on the living index of spermatozoa. 3. Conception rates in the Korean Native Cattle of pellet and ampoule semen were 55.1% and 59.2% respectively. The results indicated that conception rate of pellet semen was no better than the ampoule semen.

An optimization strategy in wind-driven circulation with uncertain forcing problem off the southeastern coastal waters of Korea

김종규,김헌태,Kim Jong-Kyu,Kim Heon-Tae 한국해양환경·에너지학회 2001 한국해양환경·에너지학회지 Vol.4 No.2

한국 남동해역 취송순환에서의 부정확한 외력의 바람응력 문제에 대한 최적화 방법의 유용성에 관하여 검토하였다. 바람응력은 모델 및 수치적 정식화 과정에서 상층 경계조건 및 외력항으로 고려되었다. 그리고 바람응력 평가에 대한 최적화 방법의 적용성 및 모델변수의 초기값 추정치의 중요성을 검토하였다. 최적화 연구로부터 동해 남부해역의 취송순환에 관한 동한난류의 수송량 변화 및 통해 난수층의 형성과 분포특성을 바람응력, 바람응력 회전성 및 상층두께의 변동으로부터 파악할 수 있음을 확인하였다. We demonstrated the importance of initial estimates of model parameters and the utility of an optimization approach of the uncertain forcing of wind-driven circulation off the southeastern coastal waters of Korea. The wind stress represents the upper boundary condition in this model and enters in the model equation as a forcing term in the numerical formalism. The wind field contributes to maintain the almost time-independent distribution of the upper layer thickness feature in a north-south direction and negative wind stress curl to maintain the formation of warm eddy off the southeastern coastal waters of Korea. Elucidated is the variational characteristics of the East Korean Warm Current due to the variations of the zonally averaged wind stress (southward transport) from the seasonal variations of the meridional transport by the Ekman transport.

여성의 손 위생관리에 관한 연구

김종규,김중순,Kim, Jong-Gyu,Kim, Joong-Soon 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.3

Objectives: This study was performed to investigate hand washing awareness of females and load of indicator bacteria on their hands. This study focused on the variation according to their age. Methods: A self-administered questionnaire survey and bacterial analysis of indicator bacteria were carried out for 100 Korean women in their age from 20 s to 60 s. Hand samples were collected through a modified glovejuice method. Results: In the survey, significant difference (p < 0.05) was found among the age groups in the use of hand washing agents. The levels of aerobic colony count (ACC) were the highest in both hands among the 20s (p < 0.05). The levels of Escherichia coli were higher in both hands in their 20s and 30s. No significant difference was found in the levels of Staphylococcus aureus and Salmonella spp. However, the positive rates of S. aureus (left hand, 37.5~47.1%; right hand, 58.5~62.5%) and Salmonella spp. (left hand, 25.0~52.9%; right hand, 37.5~64.7%) were higher in the hands of the 20s and 30s, and then showed decreasing trend according to increase of age. The effect of hand washing frequency on the ACC level of hands was significant (p < 0.001). Conclusions: These results indicate that there was no remarkable difference of hand hygiene awareness among female age groups. The detection of S. aureus and Salmonella spp. on the hands of some females in each age group revealed poor hand hygiene practices. The significant effect of hand washing frequency on the ACC level suggests that frequent hand washing is helpful to reduce hand contamination.

Aperture와 Feedline Gap 결합으로 급전되는 마이크로스트립 패치 안테나의 전자기 결합 메커니즘

김종규,윤이근,조영기,Kim, Jong-Kyu,Yoon, Lee-Geun,Cho, Young-Ki 대한전자공학회 2001 電子工學會論文誌-TC (Telecommunications) Vol.38 No.1

개구결합으로 급전되는 마이크로스트립 안테나 구조에 대해, 접지면과 패치사이의 간격에 따라 ‘cavity'형과 ’parasitic'형의 두 가지 결합 메커니즘으로 나눌 수 있음을 이론적, 실험적으로 제시하였으며, 보다 단순한 급진구조로서 gap-결합 급전 마이크로스트립 안테나구조에서도 유사한 현상이 나타남을 확인하였다. 이러한 두 가지 결합메커니즘의 특징적 차이에 대하여 논의하였다. This article describes an observation that the aperture coupling mechanisms in the aperture coupled microstrip antenna can be divided into two categories, cavity and parasitic types, depending on the separation between the microstrip patch and the ground plane. The similar phenomenon was observed in the relatively simple gap coupled microstrip antenna. The specific characteristics between two coupling mechanisms is discussed.

화학물질 관리에 대한 이해 4 - 산업보건에서 나노입자 독성에 대해 우려하는 이유

김종규,Kim, Jong-Gyu 대한산업보건협회 2011 산업보건 Vol.279 No.-

남자 대학생의 손 씻기 의식과 실천에 관한 연구

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Young,Kim, Joong-Soon 한국환경보건학회 2009 한국환경보건학회지 Vol.35 No.1

Hand-washing has been recognized as a critical factor in controlling infection and cross-contamination. This study was performed to investigate hygienic behavior of male university students focusing on awareness of hand-washing, their hand washing practices, and the difference between their awareness and practices. Both a self-administered questionnaire survey and a separate direct observation in restrooms were carried out at a university campus over a four week period. In the survey, 93.6% of the responded students claimed to wash their hands after using toilet. However, just 16.9% of the observed students actually did so, according to a separate and unnoticed observational study. Among the observed students who washed their hands, 25.0% of them used soap, only 5.0% of them washed four parts of their hands, and 15.0% of them washed their hands for more than 10 seconds. Paper towel was the most common hand drying method in the direct observation and also in the survey. Significant differences between the questionnaire survey and the direct observation (p<0.0l) were found in hand-washing practices, duration of hand-washing, using soap, washing parts of the hand, and hand-drying method after using the toilet. This study indicates that there is a remarkable difference between the male university students' awareness of hand-washing and their hand-washing practices. This study reveals that there is the need for programs or campaigns to increase hand-washing practices of male university students.

세포융합에 의한 간장 발효균의 육종

김종규,Kim, Jong-Gyu 한국식품연구원 1990 食品技術 Vol.3 No.1

일부 음식점 주방의 부유세균 및 부유진균 조사연구

김종규,박정영,김중순,Kim, Jong-Gyu,Park, Jeong-Yeong,Kim, Joong-Soon 한국환경보건학회 2014 한국환경보건학회지 Vol.40 No.2

Objectives: The purpose of this study was to determine airborne bioaerosols such as bacteria and fungi in the kitchens of restaurants, and to assess the effects of thermal factors on the levels of the bioaerosols. Methods: Air samples were taken from kitchens of nine restaurants. An Anderson type air sampler was used for sampling and measurements. Petri dishes filled with a microbiological culture medium (trypticase soy agar for bacteria and Sabouraud dextrose agar for fungi) were used as the sampling surface. Results: The levels of bacterial aerosol measured were $10-10^3CFU/m^3$ and fungal aerosol $10-10^2CFU/m^3$, respectively The mean values of air temperature and relative humidity in the kitchens were $24.6^{\circ}C$ and 46.4%, respectively. Overall, the levels of bacterial aerosol varied by the restaurant type, and fungal aerosol by the business period (p < 0.05). The main effect of air temperature and interaction effect of air temperature and relative humidity onto the bacterial level were significant (p < 0.05), whereas the effects were not significant onto the fungal level. Conclusions: The results indicate a wide variation in the levels of bioaerosols among different kitchens. The observed differences in bioaerosol levels in the kitchens reflect different periods of use. The interactions of air temperature and relative humidity onto the bacterial level suggest that constant attention should be paid to avoid peaks of contamination during the summer season.

'2002년 월드컵'을 계기로 본 "한국전통음식의 과학"

김종규,Kim, Jong-Gyu 한국과학기술단체총연합회 2001 과학과 기술 Vol.34 No.11

[전통음식/장류(된장,간장,고추장 등] 된장ㆍ간장ㆍ고추장 한국 1천5백년 전 첫 이용 폐경기 여성 노화예방 크고 항암효과도