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오존(O<sub>3</sub>) 노출에 의한 조각깔따구(Glyptotendipes tokunagai)의 체색 변화 및 heat shock protein 70 발현 변화
김원석,최보형,김문경,채선하,곽인실,Kim, Won-Seok,Choi, Bohyung,Kim, Moon-Kyung,Chae, Seon Ha,Kwak, Ihn-Sil 한국하천호수학회 2020 생태와 환경 Vol.53 No.4
오존은 수돗물 정수장에서 이용되는 소독 물질로 미세오염 물질들을 비롯해서 박테리아나 병원성 미생물체를 효과적으로 제거하는 것으로 많은 연구가 보고되어 있다. 본 연구에서는 실내 사육 중인 붉은 체색을 지닌 Glyptotendipes tokunagai를 대상으로 서로 다른 농도의 오존 노출에 따른 영향을 파악하기 위해 치사율, 체색 변화와 heat shock protein 70 (HSP70) 유전자 발현을 측정하였다. 오존에 노출된 G. tokunagai에서 농도-시간 의존적으로 치사율 증가가 관찰되었다. 또한 체색 변화는 오존 농도에 따라 붉은색의 체색이 체절마다 엷어지며 탈색되고 경직되는 현상이 보였다. HSP70 유전자 발현은 저농도인 0.2~0.5 ppm에서 노출 10분과 20분에 유의한 수준으로 높게 나타났으나(P<0.05), 30분 노출 후에는 발현량이 감소하는 경향을 보였다. 생리적으로 저산소층에 대해 적응능력이 뛰어난 깔따구 경우에도 오존은 매우 강력한 치사 효과를 유발하여 30분 노출 후 경직과 헤모글로빈 파괴로 인한 탈색이 유발되는 것을 보여주었다. 따라서 본 결과는 수돗물 정수장에서 병원성 미생물을 제거하는 데 사용되는 오존이 수생물에 주는 영향성을 파악하는 기초자료로서 활용될 수 있을 것이다. Ozone (O<sub>3</sub>) is a general disinfectant to remove micro-pollutants in water treatment system. Previous studies have reported effect of ozone to bacteria and pathogens removal, but its effect to the relatively large organisms has little known. In this study, we investigated potential effects of ozone toxicity to the non-bite midge larvae (Glyptotendipes tokunagai) with accumulate mortality, coloration change and expression of heat shock protein 70 (HSP70). The accumulate mortality rate of G. tokunagai increased in a dose-time dependent manner and the highest mortality rate was observed to 75% at 30 minute of exposure duration with 2.0 ppm of ozone concentration. Exposure to ozone was a factor increasing body color of the larvae. The tendency of HSP70 mRNA expression showed up-regulation in ozone exposure at 20 minute. After that time, the expression of HSP70 in exposed group decreased to a similar level of control group. Our results clearly showed that ozone toxicity affects physical and molecular activity of G. tokunagai, implying the potential hazardous of ozone in the aquatic ecosystem including macroinvertebrates.
염분 노출에 따른 Chironomus riparius의 성장지연과 Heat shock protein 70 유전자 발현
김원석,임병현,홍철,최승원,박기연,곽인실,Kim, Won-Seok,Im, Byeong-Hyeon,Hong, Cheol,Choi, Seung-Won,Park, Kiyun,Kwak, Ihn-Sil 한국하천호수학회 2017 생태와 환경 Vol.50 No.3
We investigate that the impact of freshwater organism exposed to the salinity environment by the frequent rainfall following climate change. To evaluate the stress response following salinity exposure, we assessed the survival rate, molting success rate, the developmental period and mouthpart deformities in Chironomus riparius. In addition, we measured the molecular responses of biomarker gene, gene expression of heat shock protein 70 (HSP70) in C. riparius exposed to salinity after 96 hour. The C. riparius survival rates were showed on time dependent manner and not observed survival organisms above 15 psu at day 4. The pupation and emergence of C. riparius were not seen above 15 psu, and the molting success rate was less than 20% at 10 psu. The developmental retardation of C. riparius was well observed in the pupation and emergence period and was delayed by 4 days at 10 psu compared to the control and 5 psu. The mouthpart deformities after salinity exposure at 96 or 72 hour were observed at 10 psu and 15 psu. The expression of C. riparius HSP70 level was significantly increased exposure to 5 psu and 10 psu. Thus, salinity has been caused to be various ecotoxicological and molecular stress responses on freshwater organisms similar to harmful substances such as EDCs and so on. 기후변화로 잦아진 강우에 의해 염분 환경에 유입되는 담수생물의 영향을 파악해 보고자 하였다. 담수의 오염지표생물인 깔따구과의 실내 실험종인 C. riparius를 대상으로 염분 노출에 따른 스트레스 반응으로 생존율과 탈피율, 성장기간, 하순기절 기형을 분석하였고, 분자생물학적으로 스트레스 분자마커로 연구가 이루어진 HSP70 유전자 발현을 측정하였다. C. riparius의 생존율은 노출시간에 따라 감소하였으며 15 psu에서 4일이 경과하면 생존개체가 관찰되지 않았다. C. riparius의 pupation과 emergence는 15 psu 이상에서는 보이지 않았고 탈피의 성공은 10 psu에서 20% 이하였다. 또한 C. riparius의 성장지연은 pupation과 emergence 기간에서 잘 반영되어, 대조군과 5 psu에 비해 10 psu에서 4일이 늦어졌다. 염분 노출 96시간 후 관찰한 C. riparius 하순기절 형태 이상은 10 psu와 15 psu에서 소수 관찰되었다. C. riparius HSP70 유전자 발현은 대조군에 비해 5, 10 psu에서 약 2.5~4배 이상 발현량이 크게 증가하였다. 따라서 염분은 담수생물에게 매우 큰 스트레스를 제공하는 원인물질로 유해물질과 유사한 생태독성학적인 반응과 분자지표 반응을 보여주었다.
김원석,김영석,전성배,전상호,이의석,장현석,권종진,임재석,Kim, Won-Seok,Kim, Young-Seok,Jeon, Seong-Bae,Jun, Sang-Ho,Lee, Eui-Suk,Jang, Hyon-Seok,Kwon, Jong-Jin,Rim, Jae-Suk 대한악안면성형재건외과학회 2012 Maxillofacial Plastic Reconstructive Surgery Vol.34 No.2
Purpose: The purpose of this study was to evaluate the expression of osteogenic genes associated with bone regeneration on anodizing titanium surface. Methods: $20{\times}20{\times}1$ (mm) commercially pure titanium plate was made, one group was pure titanium, second group was punched, and last group was punched and anodized by electrochemical method. Through the osteogenic cell culture model, the expression of extracellular matrix proteins, such as bone morphogenetic protein-2, bone sialoprotein, aggrecan, osteocalcin, Alkaline phosphatase, collagen I had been evaluated by Real-time polymerase chain reaction, and the morphology of growing cells was evaluated by scanning electron microscopy. Results: The attachment of mesenchymal stem cell was even and well-oriented on all Ti surfaces. The osteogene expression was increased on punching groups but, decreased on anodizing surfaces in 3 week samples. Conclusion: Punched anodizing Ti has possibility be using as a dental implant material, but further in vivo study would be needed.
김원석(Won-Seok Kim),조성환(Seong-Hwan Cho),윤근식(Keun-Sig Yoon) 한국산학기술학회 2024 한국산학기술학회논문지 Vol.25 No.1
If ammunition is stored for a long time, it may not fire or explode normally due to corrosion of parts or natural decomposition of chemicals or it may threaten the safety of users due to premature explosion. To prevent this, it is important to determine whether to dispose of ammunition or continue to use it by judging its shelf-life. This is also effective in increasing combat power in wartime and reducing the defense budget. The ASRP (Ammunition Stockpile Reliability Program) conducts periodic tests on stored military ammunition and analyzes and evaluates the serviceability, safety, reliability, and performance of ammunition. Its task is to determine the storage extension or disposal of ammunition according to the evaluation results. In this study, the test results of the ASRP were used for regression analysis to confirm the trend of deterioration of the function of ammunition and estimate the shelf-life. An analysis was conducted on one item, 81MM mortar cartridges, and the performance of the fuze and propellant was analyzed. The fuze and propellant are important components that determine the function of mortar cartridges. As a result of the analysis, the shelf-life of an M004 fuze is about 28 years, and a KM006 fuzes shelf-life is about 74 years. The shelf-life based on the muzzle velocity is about 89 years for M00 propellant and about 170 years for KM00 propellant. The shelf-life based on the stabilizer is about 128 years for M00 propellant and about 75 years for KM00 propellant. It is expected that the results of the study could be applied to other mortar items and it could be used to improve quality or policies for ammunition.
김원석 ( Won Seok Kim ),최창용 ( Chang Yong Choi ),장미경 ( Mi Kyeong Jang ),나재운 ( Jae Woon Nah ) 한국키틴키토산학회 2011 한국키틴키토산학회지 Vol.16 No.2
Chitosan is an attractive biomaterial for wound care because of its biocompatibility and intrinsic hemostatic and antimicrobial properties. Honey has been used since ancient times for the treatment of some respiratory diseases and for the healing of skin wounds. In this study, we investigated the effect of mixture of oligochitosan and herbal honey(chito/H) on atopic dermatitis treatment. The effect of chito/H was analyzed by clinical skin features of atopic dermatitis skin lesions, measuring the serum lgE, and investigated skin surface using an optical microscope. The scores of skin symptom level and the serum IgE were decreased after treatment with chito/H compared with durg as a control group. These results suggest that the chito/H may be an alternative substance for the treatment of atopic dermatitis.
열펌프 작동온도 변화에 따른 태양열 하이브리드 열펌프 시스템의 운전 특성 연구
김원석(Won-Seok Kim),표종현(Jong-Hyun Pyo),조홍현(Honghyun Cho) 대한설비공학회 2010 대한설비공학회 학술발표대회논문집 Vol.2010 No.6
An experimental study was carried out to investigate operating characteristics of a solar hybrid heat pump system with operating conditions. The heat pump performance was analyzed with the hot water temperature, collecting temperature and heat pump operating temperature. As a results, the hot water temperature were significantly affected by the heat pump operating temperature at the morning(time 1) and noon(time 2). The hot water temperature was set by the radiation quality and collecting operation time at the afternoon(time 3). In addition, the operating characteristics of a heat pump was significantly influenced by the heat pump operating temperature.
사무소 건물 평면의 장단변비 변화에 따른 에너지 소비 패턴에 관한 연구
김원석(Won-Seok Kim),김삼열(Samuel Kim) 대한설비공학회 2010 대한설비공학회 학술발표대회논문집 Vol.2010 No.6
This study analyzed energy consumption of office building by ECOTECT ANALYSIS, a building energy simulation program. When lateral length of building increased, energy consumption of heating and cooling has been increased. Energy losses through conduction of building's fabric was increased but energy gains through direct isolation was increased. so it showed a little difference for total consumption energy. Gain of interior lighting level has been increased when lateral of building increased. So energy consumption of electric lighting has been decreased. Resultingly when the ratio of lateral to longitudinal length change, lateral long length of a building has more disadvantage too heating and cooling energy than square shape building but we found that the total consumption of energy could be excellent through advanced lighting design, at a plan for conservative of lighting energy.