3차원 엔진 유동 모사에서의 밸브 처리에 관한 연구

김우태(W.T.Kim),허강열(K.Y.Huh) 한국자동차공학회 1995 한국자동차공학회 춘 추계 학술대회 논문집 Vol.1995 No.11_2

A simple valve treatment technique is developed to simulate multiple moving valves in three?dimensional engine flow. In this technique the convective and diffusive fluxes and the pressure gradient are put equal to zero at valve surfaces. The valve motion is treated quasi-statically. Results show reasonable trends for variation of the velocity field.<br/>

PCR - RFLP 기법을 이용한 젖소 유전적 개량을 위한 선발도구로서 β- lactoglobulin 좌위의 유전자형 분석

정의룡(E . R . Chung),김우태(W . T . Kim),한상기(S . K . Han) 한국축산학회 1994 한국축산학회지 Vol.36 No.6

Genotypes of the β-lactoglobulin(β-LG) locus as a generic marker linked to quantitative trait loci affecting traits of economic importance in dairy cattle were determined by the PCR-RFLP or AFLP method. Genomic DNA was prepared from blood of Holstein cows. The PCR was used to amplify a 262 by region between nucleotides 367 and 629 from exon IV to intron N of the bovine β-LG gene using forward primer(GTCCTTGTGCTGACACCGACTACA-3`) and reverse primer(CAGGACACCGGCTCCTGGTATATGA-3`). After amplification, PCR products were digested with Hae III restriction enzyme, and the fragments were separated by agarose gel electrophoresis for RFLP or AFLP analysis of β-LG locu s. RFLP specific for the β-LG A and B alleles were identified with the Hae III restriction enzyme. The β-LG AA genotypes produced two fragments of 109 and 153bp and the BB genotypes three fragments of 109, 79 and 74bp. The AB genotypes showed the intermediate pattern. Thus, PCR amplification and RFLP analysis was shown to be a rapid and sensitive method for the discrimination of β-LG genotypes directly at the DNA level in dairy cattle of any age or sex. Consequently, the PCR-RFLP or AFLP method presented in this study can be used as a valuable tool for early selection of A1 bulls and calves with the desirable β-LG gene or BB genotype affecting superior milk production traits for genetic improvement of dairy cattle.

RAPD - PCR 기법을 이용한 젖소의 DNA 다형분석과 유전적 특성에 관한 연구

정의룡(E . R . Chung),김우태(W . T . Kim),한상기(S . K . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.5

This study was carried out to detect DNA polymorphisms in domestic Holstein dairy cattle using random amplified polymotphic DNA(RAPD) analysis, based on the amplification of random DNA sequences with arbitrarily chosen primers by the PCR-technique, to analyze genetic variation and characteristics within the breed and to develop the breed-specific genetic markers by using these RAPD markers. Preliminary RAPD analysis was performed on genetic DNA samples from a total of 30 Holstein cows and 10 Korean native cattle as a reference breed using 40 different random primers. Of these, sixteen primers for further investigation were selected based on the number and frequency of the polymotphisms produced. Using 10 single-primers, a total of 78 RAPD markers were produced, seventy eight(`74.4%) bands of which were polymorphic, whereas twenty (25.6%) bands were monomorphic. The average bandsharing was 0.353 within the Holstein breed. Each of the primers generated RAPD banding patterns different in both size, number and intensity of amplified fragments. A specific primer (P-9) was found to be useful in the individual identification, resulting from the different DNA polymorphism among individuals. Two of the primers, P-5 and P-10, produced Holstein breedspecific RAPD markers when compared with those from the Korean native cattle. The RAPD profiles using polyacrylamide gel were different from those obtained in the agarose gel. The separation of the fragments with low molecular weight was more apparent in the polyacrylamide gel. The pairs of primers produced more or less DNA bands amplified than the single primers, depending on the composition of the two primer sequences combined.

젖소의 주요 조직합성 복합체 ( MHC ) 로서 BoLA - DRB 3 유전자의 DNA 다형현상

정의룡(E . R . Chung),김상균(S . K . Kim),김우태(W . T . Kim) 한국낙농학회 1998 韓國酪農學會誌 Vol.20 No.4

N/A N/A

PCR - RFLP 기법을 이용해 젖소개량을 위한 유전적 표지로서 K- Casein 좌위의 유전자형 분석

정의룡(E . R . Chung),김우태(W . T . Kim),최석호(S . H . Choi),임태진(T . J . Rhim),한상기(S . K . Han) 한국축산학회 1995 한국축산학회지 Vol.37 No.1

Genotypes of K-casein(K-CN) locus as a genetic marker linked to quantitative trait loci affecting traits of economic importance in dairy cattle were determined by PCR-RFLP method. Genomic DNA was prepared from blood of Holstein cows. The PCR was used to amplify an 874 by region between nucleotides 10592 and 11466 from exon IV to intron IV of the bovine K-CN gene using sense primer(5`-GTGCTGAGTAGGTATCCTAG-3`) and antisense primer(5`GTAGAGTGCAACAACACTGG-3`). After amplification, PCR products were digested with four restriction enzymes, Hind III, Rsa I, Taq I, and Pst I, and the fragments were separated by agarose gel electrophoresis for RFLP analysis of K-CN locus. In addition to screening for the known Hind III and Rsa I restriction site polymorphisms of K-CN locus, we have found additional RFLPs specific for the K-CN A and B alleles in Taca I and Pst I enzymes. The amplified DNA product digested with each restriction enzyme generated specific RFLP pattern that allowed precise identification of K-CN AA, BB or AB genotypes. The K-CN genotypes determined for cows by the PCR-RFLP method agreed completely with the phenotypes obtained from milk samples of the same individuals. Thus, PCR amplification and RFLP analysis was shown to be a rapid and sensitive method for the discrimination of K-CN genotypes directly at the DNA level in dairy cattle of any age or sex. Consequently, the PCR-RFLP method presented in this study can be used as a valuable tool for early selection of AI bulls and calves with desirable K-CN B gene or K-CN BB genotype affecting superior milk production traits for genetic improvement of Holstein dairy cattle.

RAPD - PCR 기법을 이용한 젖소의 친지판정 및 가계분석에 관한 연구

정의룡,김우태,김연수,김동균 ( E . R . Chung,W . T . Kim,Y . S . Kim,D . K . Kim ) 한국낙농학회 1998 韓國酪農學會誌 Vol.20 No.1

국내 인공수정용 젖소 종모우 집단에 있어 κ-Casein 과 β-Lactoglobulin 유단백질의 DNA 유전자형 분석

정의룡,김우태,김연수,김동균 ( E . R . Chung,W . T . Kim,Y . S . Kim,D . K . Kim ) 한국낙농학회 1998 韓國酪農學會誌 Vol.20 No.2

이온프로브를 이용한 가솔린엔진의 화염전파측정에 관한 연구

한봉훈(B.H.Han),김우태(W.T.Kim),박심수(S.S.Park),이현순(H.S.Lee) 한국자동차공학회 1992 한국자동차공학회 춘 추계 학술대회 논문집 Vol.- No.-

The in-cylinder combustion phenomena of internal combustion engine have been the subject of many researches. However, a number of early studies were carried out by using the combustion pressure measurement or the visualization of flame propagation with modifications of engine hardware or rig equipments and thus their applications to engine development process were rather I imi ted. In order to investicate the<br/> flame propagation of the gasoline engine wi thout any modi fications of<br/> <br/> engine hardware, the ion probes are installed at cylinder head.<br/> <br/> The<br/> <br/> measurement technique is based upon the ion current detecting method. The applicability of the ion probe sensor is confirmed with a-engine<br/> at different engine operating parameters.<br/> The test results indicate that the ion probe sensor provides enough information of mean flame propagation speed and flame behaviors in the<br/> combustion chamber.<br/>

DNA 표지인자를 이용한 한국 재래산양의 품종식별

최순호,김계웅,정의룡,김우태,김연수,한상기 한국축산학회 1999 한국축산학회지 Vol.41 No.3

7Mis study was conducted to analyze genetic characteristics and to develop the breed-specific DNA marker for breed identification of Korean native goat(KNG) as an indigenous animal genetic resource using random amplified polymorphic DNA(RAPD) and DNA amplification fingerprinting(DAF) techniques. A total of 30 KNG wide the high phenotypic purity and five exotic breeds including imported black goat(1BG) as a meat type breed and Saancn(SAA). Alpine(ALP), Nubian(NUB) and Toggenburg(TOG) as dairy type breeds were used in this study. Ten individuals were sampled from each exotic breed. Genomic DNA derived from blood or milk samples was amplified by PCR using random primers. Thirty different l0-base oligonucleotide primers were used in the initial screening. Of the 30 arbitrary selected primers, twelve primers showing highly polymorphic and reproducible amplification patterns were chosen for further analysis of RAPD and DAF. A total of 75 RAPD markers were produced, 36(48.0%) bands of which were polymorphic, compared to a total of 184 DAF markers with 117(64.0%) polymorphic bands. Therefore, the amount of polymorphism detected by DAF is much greater than that of RAPD. The band sharing value (62%) in the KNG was the highest among goat breeds, indicating that genetic similarity within KNG population was higher than those of other goat breeds. Two of the primers. GEN-01(GGTGACGCAG) and GEN-08(ACCCCCGAAG), produced breed-specific DNA bands showing differences between breeds. The 1.80kb band of GEN-O1 primer and 1.0kb band of GEN-08 primer were present in all of the dairy goat breeds examined including SAA, but not present in the meat type breeds of KNG and IBG. On the contrary, the 0.70kb band of GEN-Ol and 0.60kb band of GFN-08 were found in all of the KNG, but not in the IBG nor any of the dairy goat breeds. Therefore, these DNA bands specific for KNG may be useful putative breed-diagnostic markers to discriminate KNG from other improved goat breeds. In comparison of genetic distance and dendrogram, the KNG was the most closely related to the IBG, followed by the SAA. In conclusion. the RAPD and DAF markers will be useful for estimating genetic variation and relationships in goat breeds and for the breed identification of KNG.

AFLP Marker 를 이용한 한우의 유전자 지문 분석

정의룡,김우태,김연수,한상기 한국동물자원과학회 2000 한국축산학회지 Vol.42 No.4

This study was conducted to investigate genetic variability and to develop the breed-specific DNA markers of Hanwoo (Korean cattle) and to estimate phylogenetic relationships among Hanwoo, Chinese Yanbian cattle and other foreign cattle breeds (Holstein, Angus, Charolais and Hereford) using AFLP markers as a new DNA fingerprinting technique. Genomic DNA was digested with a particular combination of two restriction enzymes with 4 base (Mse I and Taq I) and 6 base (EcoR I and Hind III) recognition sites, ligated to restriction specific adapters and amplified using the selective primer combinations. In the Hanwoo breed, the number of scorable AFLP bands detected per primer combination varied from 37 to 102, with an average of 66.1 using 13 primer combinations. A total of 859 markers were generated and 568 bands were polymorphic (66%). Among the primer combinations, E35/H12, M11/T32, E38/H13 and E32/H11 primer combinations showed a high level of polymorphism with 0.93, 0.89, 0.86 and 0.83, respectively. Therefore, these primer combinations were effective for AFLP analysis of Hanwoo. The level of polymorphism of Hanwoo was similar to that of Chinase Yanbian cattle (65%) and higher than those of other foreign cattle breeds (48% to 55%). E35/H32 and T32/M13 primer combinations generated AFLP fingerprinting showing individual-specific of Hanwoo. M13/H13 and T32/M13 primer combinations produced breed-specific DNA markers when compared AFLP DNA bands between breeds. Three bands (0.84kb, 0.70kb and 0.64kb) identified in M13M13 primer combination and two bands (0.25kb and 0.24kb) identified in T32/M13 primer combination were found to be unique in Hanwoo. Therefore, these AFLP DNA bands could be used as putative breed-specific DNA markers for Hanwoo breed identification. In comparison of genetic distances, Hanwoo was the most closely related to the Chinase Yanbian cattle, followed by the Hereford and Holstein breeds. AFLP fingerprints may be useful for parentage testing, pedigree analysis, genetic variability and relationships and for the development of DNA markers linked to economic traits in Hanwoo.