Synthesis of New Anthracycline Derivatives Including Butyric or Retinoic Acid Moiety.

김완중,박시호,유동진,강흔수,정순량,Kim, Wan Jung,Park, Si Ho,Yu, Dong Jin,Gang, Heun Su,Jeong, Sun Ryang 대한화학회 2001 Bulletin of the Korean Chemical Society Vol.22 No.6

The potential anticancer agents, new anthracycline analogues (2-9) have been synthesized from the glycosides daunomycin (1a) and doxorubicin (1b). Compounds 2 and 6 were prepared by nucleophilic displacement esterification of a 14-bromodauomycin(1c) with sodium or potassium salts of butyric and all trans retinoic acid, respectively. Compounds 3 and 7 were obtained from daunomycin (1a) by direct amidation with a butyric and all trans retinoic acid in the presence of EDCI and PP, respectively. Compounds 4 and 8 were obtained from doxorubicin (1b) by reaction with the corresponding acids in the same manner. Compounds 5 and 9 were prepared from doxorubicin (1b) by acylation with two equivalents of the corresponding acids under the same reaction conditions.

Synthesis of New Anthracyline Derivatives Containg Acetylsalicyclic or Palmitic Acid Moiety.

김완중,박시호,유동진,강흔수,정순량,Kim, Wan Jung,Park, Si Ho,Yu, Dong Jin,Gang, Heun Su,Jeong, Sun Ryang 대한화학회 2001 Bulletin of the Korean Chemical Society Vol.22 No.6

The potential anticancer agents new anthracycline derivatives (2~9) have been synthesized from daunomycin (1a) and doxorubicin (1b) ad starting materials. Compounds 2 and 6 were prepared by the nucleophilic displacement type esterification of a 14-bromodaunomycin (1c) with a acetylsalicylic and palmitic acid in triethylamine, respectively. Compound 3 and 7 were obtained from daunomycin (1a) by direct amidation with the corresponding acids in the presence of EDCI and PP as esterification regents. Whereas 4 and 8 were prepared by reaction of doxorubicin (1b) with one equivalent of acetylsalicylic and palmitic acid using DCC/DMAP, respectively, 5 and 9 were obtained from 1b by acylation with two equivalents of the corresponding acids using EDCI/PP reagents.

Synthesis and Antitumor Activity of New Anthracycline Analogues

김완중,유동진,강현수,장순량,Kim, Wan Jung,Yu, Dong Jin,Gang, Hyeon Su,Jang, Sun Ryang 대한화학회 2001 Bulletin of the Korean Chemical Society Vol.22 No.9

New anthracycline analogues 2-9 as potential anticancer agents have been synthesized from daunomycin (1a) and doxorubicin (1b). Compounds 2 and 6 were prepared by the nucleophilic displacement type esterification of 14-bromodaunomycin (1c) with N-benzoyl-(2R,3S)-phenylisoserine and L-pyroglutamic acid in triethyl-amine, respectively. Compounds 3, 7 and 4, 8 were prepared by the reaction of either daunomycin (1a) or doxorubicin (1b) with one equivalent of the corresponding acids in the presence of EDCI/PP. Compounds 5, 9 were obtained from 1b by reaction with 2.2 equivalents of the corresponding acids in the same manner. The cytotoxic activities of the analogues in comparison with adrimycin on cultured SNU-16 and MCF7 cell were described.

산화스트레스에 의해 유도된 근세포 손상에서 심비디움 뿌리추출물의 효과

김완중(Wan Joong Kim),김한성(Han-Sung Kim),오피츠 요크(Joerg Opitz),가바야마 카즈야(Kazuya Kabayama),김택중(Tack-Joong Kim) 한국생명과학회 2014 생명과학회지 Vol.24 No.9

근위축은 근육을 사용하지 않음으로써 발생하는 근육 조직의 손실 또는 근육을 지배하는 신경의 손상으로 정의할 수 있다. 이 상태는 다양한 근육질환에 관여하는 활성산소종이 관여 한다. 우리는 심비디움 뿌리 추출물이 과산화수소에 의해 유도된 C2C12 근육세포 생존율 손실과 세포사를 억제한다는 것을 찾았다. 또한 심비디움 뿌리추출물이 HSP70 단백질 발현증가와 SOD1 단백질 발현감소를 확인하였다. 이들의 결과는 심비디움 뿌리 추출물이 근위축에서 활성산소종을 환원하는 치료약물로서 작용이 가능할 것으로 사료된다. Skeletal muscle atrophy can be defined as a decrease in or a disease of the muscle tissue, or as a disorder of the nerves that control the muscle, through injury or lack of use. This condition is associated with reactive oxygen species (ROS), resulting in various muscular disorders. Exposure to ROS induces muscle atrophy through several biological factors, such as SOD1 and HSP70. We found that cymbidium root extract reduced the H₂O₂-induced viability loss in C2C12 myoblasts and inhibited apoptosis. In addition, we showed that the cymbidium root extract increased the expression of HSP70 and decreased the expression of SOD1 in the H₂O₂-induced C2C12 myoblasts. These results suggest that cymbidium root extract might have therapeutic value in reducing ROS-induced muscle atrophy.

직접 용액 축중합에 의한 Poly(lactic acid-co-mandelic acid) 의 합성 및 특성 조사

김완중(Wan Jung Kim),김지흥(Ji Heung Kim),김수현(Soo Hyun Kim),김영하(Young Ha Kim) 한국고분자학회 2000 폴리머 Vol.24 No.3

직접 용액 축중합에 의한 직쇄형 및 스타형 폴리락트산의 합성과 물성

김완중(Wan Jung Kim),이선영(Sun Young Lee),김수현(Soo Hyun Kim),김지흥(Ji Heung Kim) 한국공업화학회 1999 공업화학 Vol.10 No.7

N/A N/A

대장암에서 종양억제 유전자와 p53의 상위조절인자로서의 hSRBC 연구

김완중 ( Wan Jung Kim ),김효종 ( Hyo Jong Kim ),지성길 ( Sung Gil Chi ),김진오 ( Jin Oh Kim ),조주영 ( Joo Young Cho ),심찬섭 ( Chan Sup Shim ) 대한장연구학회 2007 Intestinal Research Vol.5 No.2

Background/Aims: hSRBC [human Serum deprivation response (sdr)-Related gene product that Binds to c-kinase] was identified using PKCδ or BRCA1 as a probe and located at 11p15.5-p15.4 region. Expression of hSRBC protein was also decreased in a number of breast, lung, and ovarian cancer cell lines, suggesting that hSRBC might be a putative tumor suppressor gene. Methods: The expression status of hSRBC was analyzed in 50 primary colon tumors and their adjacent 50 normal tissues, and 20 colon cancer cell lines. Transcript and protein expression of hSRBC was studied by quantitative RT-PCR and Western blot, respectively. siRNA-mediated knockdown of hSRBC expression was utilized to investigate its association with p53. Results: The mRNA expression of hSRBC was decreased in 60% (12/20) of colon cancer cell lines and 44% (22/50) of patient``s colon cancer tissues. Expression of hSRBC mRNA was significantly decreased in tumors compared to non-cancerous cells, while genomic level of hSRBC was not decreased in tumors. hSRBC expression was increased by 5-aza-2’-deoxycytidine treatment and hypermethylation of CpG sites was strongly associated with decreased expression. Ectopic transfection of hSRBC suppressed RKO cell count and hSRBC knockdown by siRNA augmented HCT116 cell numbers. Flow cytometry showed G1 arrest and apoptosis of colon cancer cells by restoration of hSRBC expression in RKO cells. Both basal and etoposide-mediated p53 expression was decreased when hSRBC expression was knockdowned with siRNA. Conclusions: hSRBC expression is frequently decreased by promoter CpG site hypermethylation. hSRBC down-regulates p53 expression in G1 phase and might be a novel upstream regulator of p53. (Intest Res 2007;5:131-143)

당뇨병 환자에서 RR 간격의 변화에 관한 연구

김완중(Wan Jung Kim),김중남(Jung Nam Kim),김호중(Ho Jung Kim),문철웅(Chul Oong Moon),이승일(Soung Il Lee),장경식(Kyoung Sig Chang),홍순표(Soon Pyo Hong) 대한내과학회 1990 대한내과학회지 Vol.39 No.4

N/A Heart rate variability is a noninvasive index of the neural, especially parasympathetic, activity of the heart. Autonomic neuropathy is frequently observed in diabetic patients, and a 5-year survival rate of this kind of patient is low. To evaluate autonomic neuropathy in diabetic patients, 35 diabetic patients (proliferative retinopathy: 14, neurogenic bladder:8, postural hypotenion:6) measured for RR intervals and Valsalva ratios compared with 77 persons of the normal control group. RR variations (standard deviation: SD, coefficient of variation: CV, max-min, max/min) during deep respiration and Valsalva ratios (Vmax-Vmin, Vmax/Vmin) during Valsalva maneuver were significantly lower in the diabetic patients than in the normal control group (p<0.001, respectively). RR variations and Valsalva ratios decreased progressively with an increase of age in the control group and in diabetic patients (p<0.001, respectively). RR variations and Valsalva ratios were significantly lower in the diabetic patients with retinopathy, neurogenic bladder, and postural hypotension compared to diabetic patients without such conditions (P value <0.01, <0.05, <0.05, respectively). It is concluded that diabetic autonomic neuropathy can be indicated by the measurement of RR variations and Valsalva ratios.

내시경점막하박리술로 치료한 조기위암에서 현미부수체 불안정성의 의의

김경민 ( Kyoung Min Kim ),김연수 ( Yeon Soo Kim ),조주영 ( Joo Young Cho ),정인섭 ( In Sup Jung ),김완중 ( Wan Jung Kim ),최익성 ( Ik Seong Choi ),유창범 ( Chang Beom Ryu ),김진오 ( Jin Oh Kim ),이준성 ( Joon Seong Lee ),진소영 ( 대한소화기학회 2008 대한소화기학회지 Vol.51 No.3

목적: DNA 불일치 교정 기전에 문제가 발생하면 현미부수체 불안정성(microsatellite instability, MSI)이 발생한다. 내시경점막하박리법으로 치료한 조기위암에서 현미부수체 불안정성의 의의를 알아보기 위해 현미부수체 불안정성과 임상요인 및 암종의 조직형과 표현형, 그리고 혈관내피성장인자, p53 발현과의 관계를 조사하였다. 대상 및 방법: 2005년 5월부터 2006년 11월까지 순천향대학교 서울병원에서 조기위암으로 내시경점막하박리법을 시행받은 94명의 환자를 대상으로 하였다. 현미부수체 불안정성을 확인하기 위하여 BAT25, BAT26, D2S123, D5S36, D17S250를 표지자로 하여 중합효소연쇄반응을 시행하였다. 연령, 성별, p53 발현율, 혈관내피성장인자 발현율, 암종의 침습 정도, 암종의 위치, 이시 및 동시 암의 발생률 등을 현미부수체 불안정성과의 연관성을 비교하였다. 결과: 현미부수체 불안정성은 총 95예 중 13예(13.7%)에서 보였다. 여성은 전체에서 28예(29.5%)였으며 이 중 현미부수체 불안정성은 28.6% (8/28예)로 남성에서의 7.5% (5/75예)보다 유의한 차이를 보이고 있었다 (p=0.006). 암의 위치에 따라 위각부와 전정부에서 발생한 경우(n=73)에서 현미부수체 불안정성의 발현은 13예로 17.8%에서 보이고 있었으며 위체부 상방에서 발생한 경우 (n=22)에서는 현미부수체 불안정성의 발현이 나타나지 않아 유의한 차이를 보였다. 그밖에 조직학적인 요인들과 현미부수체 불안정성 발현과는 통계적인 차이가 없었다. 결론: 이 번 연구에서는 현미부수체불안정성인 경우 암이 위의 원위 부에 잘 생기며, 여성에서의 발현율이 남성에 비해 높은 것으로 나타났으나 그 밖의 다른 인자들과의 연관성은 찾지 못하였다. 추후에 이 환자들에서 장기 예후 평가나 재발률에 관한 연구가 추가될 수 있으며 위암에서의 현미부수체 불안정성의 의미에 대한 더 많은 연구가 필요하다. Background/Aims: Microsatellite instability (MSI) is defined as a change of any length due to either insertion or deletion of repeating units, in a microsatellite within a tumor when compared to normal tissue. MSI is closely related with genetic instability, particularly in hereditary nonpolyposis colorectal cancer. MSI is found in 10-50% of all gastric cancers, suggesting that MSI may play an important role in carcinogenesis. The aim of this study was to investigate the relationship between microsatellite instability and clinicopathologic features in early gastric cancers (EGCs) treated by endoscopic submucosal dissection (ESD). Methods: We analyzed clinicopathological features of 95 specimens of EGCs including MSI, histologic type, mucin phenotype, p53, VEGF, location of cancer, depth of invasion, incidence of synchronous and metachronous cancer, age, and gender derived from 94 patients, treated by ESD during recent 19 months were analyzed in this study. Results: According to microsatellite stability, MSI was observed in 13 (13.7%) cases of 95 specimens. The incidence of MSI was increased in patients with cancer at lower part of stomach and female gender. There was no significant relation between MSI and clinicopathologic features including histologic type, mucin phenotype, p53, VEGF, and depth of invasion. Conclusions: Our results demonstrate that there is no relationship between MSI and clinicopathologic features except tumor location and gender in ECGs treated by ESD. However, further studies are needed to evaluate the significance of MSI in EGCs. (Korean J Gastroenterol 2008;51:167-173)

인도네시아의 교역조건과 투자 관계

김완중(Wan Joong Kim) 한국아시아학회 2014 아시아연구 Vol.17 No.1

인도네시아 경제의 특징은 자원의존적 경제구조를 가지고 있다는 것이다. 본 연구에서는 자원의존적 경제인 인도네시아에서 교역조건 개선이 투자에 긍정적인 영향을 미쳤는지의 여부를 ARDL모형을 이용하여 분석한다. 1980-2010년 시계열자료를 분석한 결과, 인도네시아에 있어서 교역조건개선은 장기와 단기에 있어서 모두 투자에 긍정적인 영향을 주지 못하는 것으로 나타났다. 이러한 결과는 수출재가 노동집약재인 경우 교역조건의 개선은 투자를 증가시키기 보다는 감소시킬 수 있다는 마추야마(Matsuyama 1988) 이론, 자원의존적인 국가에 있어서 교역조건의 개선은 경제에 부정적인 영향을 줄수 있다는 지대이론, 그리고 ‘네덜란드병(Dutch desease)’ 가설 등과 관련이 있는 것으로 보인다. As well known, Indonesia is a resource-dependent country. In this paper, It is tested that improvement of terms-of-trade positively affects the economy. Based on ARDL analysis, I found that terms-of-trade could not improve investment(fixed capital formation) of the country in the short run and in the long run. Those results can be understood in some economic theories such as Matsuyama`s theory(1988), rent-seeking theory, and hypothesis of ``Dutch desease``. On the other hand, it is found that economic growth is a major determinant of Indonesian investment.