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맥각균의 배양에 관한 연구(III) - 발효조에서의 Lysergic Acid 알칼로이드의 생성
김병각,옥선,최응칠,Kim, Byong-Kak,Auck, Sun,Choi, Eung-Chil 생화학분자생물학회 1973 한국생화학회지 Vol.6 No.3
맥각균을 발효조에서 배양할때 라이서직산 알칼로이드의 생성에 미치는 배양조건 및 첨가물질의 영향을 검토하기 위하여 Claviceps paspali Stevens and Hall 31호 균주를 Schwarting 배양액에서 배양하였으며 Tween 80, 분자상의 산소, 디메칠설폭사이도, 비산 나트륨을 각각 첨가했을 경우 및 솔비톨-푸마르산 배양액과 프로펠라형 교반기를 사용했을 경우의 영향을 실험하였다. 알칼로이드 총량의 정량은 Michelon-Kelleher 법을 이용하였다. 실험결과에 의하면 산소, 디메칠성폭사이드, 비산염의 각각 첨가 및 솔비톨-프마르산 배양액의 사용이 알칼로이드 생성을 증가하였다. 그러나 Tween 80의 첨가는 과잉의 포말을 발생시켜 배양액의 손실을 초래하였으며 결국 알칼로이드의 축적이 감소되었다. Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No.31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.
한국산 고등 균류의 성분 연구 (제37보) : 뽕나무버섯의 항암 성분 Antitumor Components of Armillariella mellea
김병각,최응칠,심미자,정경수,이정옥,김혜령,이종길,김진숙 한국균학회 1983 韓國菌學會誌 Vol.11 No.4
To find antitumor components in Korean basidiomycetes, the carpophores of Armillariella mellea which were collected in Gyeong Gi Province were extracted with distilled water at 90∼100℃ for eight hours. The hot water extract was concentrated under reduced pressure, mixed with three-fold volumes of ethanol and allowed to stand at 4℃ overnight. The precipitate was centrifugated and lyophilized to yield a protein-polysaccharide fraction. It was examined for antitumor activity against sarcoma 180 implanted in ICR mice. The fraction showed 75.7%, 83.9% and 94.1% of tumor inhibition ratios at the doses of 10, 20 and 50 ㎎/㎏/day, respectively. The chemical analysis of the fraction showed that it contained a polysaccharide(41.3%) and a protein(35.0%). The hydrolyzates of the polysaccharide moiety contained fucose(4.5%), xylose (1.1%), galactose(17.4%), glucose(55.4%), mannose(19.4%), and one unknown monosaccharide. The protein moiety contained seventeen amino acids. The protein-polysaccharide from A. mellea was administered, i.p., to mice and caused an influx of polymorphonuclear leukocytes (PMN) at 5∼24 hours which was followed by an accumulation of macrophages and disappearance of the PMN at 48∼72 hours.
맥각균의 배양에 관한 연구 ( 3 ) 발효조에서의 Lysergic Acid 알칼로이드의 생성
김병각,옥선,최응칠 ( Byong Kak Kim,Sun Auck,Eung Chil Choi ) 생화학분자생물학회 1973 BMB Reports Vol.6 No.3
Ergot fungus was culitvated in stirred fermentors in various modified conditions to investigate their influence on the production of lysergic acid alkaloids. Claviceps paspali Stevens and Hall Isolate No. 31 was cultured in mannitol-succinate medium of Schwarting, et al., and such modifications as additions of Tween 80, oxygen gas, dimethylsulfoxide, arsenate and the utilization of sorbitol-fumaric acid medium and propeller-type impellers were respectively examined. Quantitative determination of the total alkaloids was carried out using Michelon-Kelleher method. The results showed that additions of oxygen gas, dimethylsulfoxide, arsenate, and the utilization of sorbitol-fumaric acid medium increased the production of the alkaloids. However, the addition of Tween 80 caused extensive foaming and resulted in decrease of the alkaloid accumulation.
김병각,황성현,최응칠,권창호 ( Byong Kak Kim,Sung Hyun Whang,Eung Chil Choi,Chang Ho Kwon ) 생화학분자생물학회 1974 BMB Reports Vol.7 No.1
Korean ergot sclerotia which were collected from Agropyron semicostatum Nees, Miscanthus sinensis Anders. var. purpurascens Rend., M. sacchariflorus Benth. et Hook., M. sinensis Anders., Eulalia speciosa O. Kuntz, Themeda triandra Forsk. var. japonica Makino, and Phalaris arundinacea L. were investigated for their alkaloid constituents. Quantitative determinations of alkaloids were carried out using a modified procedure of van Urk and Michelon-Kelleher methods. The results showed that the two ergots from Agropyron semicostatum and Phalaris arundinacea contained total alkaloids up to 0. 137 per cent. The alkaloids of these two ergots are mostly water-insoluble alkaloids. The ergot parasitizing Reed canary grass was found to contain both water-soluble and water-insoluble alkaloids. The TLC and PPC tests for the ergot alkaloids from Agropyron semicostatum and Phalaris arundinacea revealed that they contain at least two alkaloids respectively and that the ergot from Themeda triandra Forsk var. japonica has one. The lipid contents of the seven ergots ranged from 13 to 29 per cent.