2009 개정 교육과정에 따른 중학교 과학 교과서의 화학 영역과 고등학교 화학 교과서에 사용된 비유의 분석

김경순,안인영,최용남,노태희,Kim, Kyungsun,Ahn, Inyoung,Choi, Yongnam,Noh, Taehee 대한화학회 2013 대한화학회지 Vol.57 No.6

In this study, we analyzed the analogies in the chemistry domain of 27 middle school science textbooks and 8 high school chemistry textbooks developed under the 2009 Revised National Curriculum. A total of 284 analogies (science, 143; chemistry, 141) were identified from the textbooks, which means that 8.11 analogies (science, 5.3; chemistry, 17.6) per textbook and 0.68 analogy (science, 0.66; chemistry, 0.70) per 10 pages were used on average. Compared with previous curricula that the number of analogies gradually increased, the use of analogy was found to be somewhat decreased in the 2009 Revised National Curriculum. The number of analogies found in each textbook considerably varied depending on course, unit, and publishing company. One hundred ninety one different kinds of analogies were used, among which 38(19.9%) were used over twice. Further analyses of the types of analogies indicated that verbal and pictorial analogy, analogy with abstract target and concrete analog, and enriched analogy were frequently used. However, the term 'analogy' and the description about the limitation of the analogy were rarely mentioned. Artificial analogy, teacher-centered analogy, and analogy with low systematicity were also found to be frequently used. Educational implications of these findings are discussed. 이 연구에서는 2009 개정 교육과정에 따른 중학교 과학 교과서 27권의 화학 영역과 고등학교 화학 교과서 8권에서 사용된 비유의 빈도와 유형을 분석하였다. 분석한 교과서에 사용된 비유는 총 284개(과학, 143개; 화학, 141개)로서, 교과서 한 권당 8.11개(과학, 5.3개; 화학, 17.6개), 10쪽마다 평균 0.68개(과학, 0.66개; 화학, 0.70개)의 비유가 사용되었다. 비유의 빈도는 이전 교육과정까지는 꾸준히 증가하는 추세였던 것과는 달리 2009 개정 교육과정에서 다소 감소하였다. 또한, 교과목별, 단원별, 출판사별로 사용된 비유의 빈도에 차이가 나타났다. 비유물의 종류는 총 191종(과학, 81종; 화학, 110종)이었고, 각 교과목별로 2번 이상 공통으로 사용된 비유물은 38종(19.9%)이였다. 유형별로 비유를 분석한 결과, 그림과 언어를 같이 이용한 비유 및 추상적 개념을 구체적 비유물에 대응시키는 비유가 주로 사용되었고, 공유 속성을 설명해 주는 부연 비유의 사용이 많았다. 그러나 비유임을 명시하거나 비유물의 제한점에 대해 언급한 비유들이 거의 없었고, 체계성이 낮은 비유나 작위적 상황을 적용한 비유 및 교사 중심의 비유가 많이 사용되는 것으로 나타났다. 이 연구의 결과에 대한 교육적 함의를 논의하였다.

귀전우, 백화사설초, 와송 추출물을 처치한 난소암과 자궁경부암 세포에서의 단백질 발현 변화

김경순,예성철,유병철,조종관,이연월,유화승,Kim, Kyung-Soon,Yea, Sung-Chul,Yoo, Byong-Chul,Cho, Chong-Kwan,Lee, Yeon-Weol,Yoo, Hwa-Seung 대한한방내과학회 2011 大韓韓方內科學會誌 Vol.32 No.1

Background : Despite recent advances in cancer management, prognosis of ovarian cancer is poor. Anticancer effects of herbal medicine, such as Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh, and Orostachys japonicus A. Berger, have been reported in treatment of ovarian and cervical cancers, but the systematic approaches to explain their molecular mechanism(s) have not yet been established. Objectives : To establish a basis of understanding for anti-cancer mechanisms of herbal medicine, we profiled protein expression in human ovarian and cervical cancer cells treated with the extracts from Euonymus alatus Sieb, Oldenlandia diffusa (Willd.) Roxburgh and Orostachys japonicus A. Berger. Methods : Human ovarian cancer cell line NIH:OVCAR-3, and human cervical cancer cell line HeLa were employed in the present study. Whole protein was obtained from the cells harvested at 48 hours after the treatment with herbal water-extract, and analyzed by 2DE-based proteomic approach. Results : Various changes of protein expression induced by the herbal treatment were monitored : down-regulation of molecular chaperone (calreticulin variant), glycolytic enzymes (D-3-phosphoglycerate dehydrogenase, glyceraldehyde 3-phosphate dehydrogenase and alpha-enolase), RNA processing molecules (hnRNP A2/B1), and antioxidant protein (peroxiredoxin 1). Conclusions : Repression of glycolysis has been accepted as the mechanism to increase anticancer reagent's effect. Thus, down-regulation of glycolytic enzymes by the herbal extracts suggested a possible synergistic effect of herbs in the presence of platinum-based therapeutics. In further study, as well as the synergistic effect of the herbs, it has to be further validated whether artificial regulation of hnRNP A2/B1 in ovarian cancer cells affects various cancer survival factors, since RNA processing can be interrupted by deranged expression of hnRNP subtypes, and it results in an inhibition of cancer cell growth.

모유수유와 분유수유에 따른 영아 장내 미생물 군집의 특징

김경순,신정,심지수,연수지,이평안,정문규,Kim, Kyeong Soon,Shin, Jung,Sim, JiSoo,Yeon, SuJi,Lee, Pyeong An,Chung, Moon Gyu 한국미생물학회 2019 미생물학회지 Vol.55 No.3

장 내에 존재하는 microbiome은 생활환경, 나이와 섭취하는 음식물에 따라 변하게 된다. 본논문에서는 모유를 섭취한 영아와 분유를 섭취한 영아들에 대한 장내미생물 군집의 변화를 비교하였다. 미생물의 군집변화를 차세대 염기서열 분석기법을 이용하여 분석하였으며 총 80개의 영아 분변을 통해 미생물 군집변화를 확인하였다. 모유그룹(BIG)과 분유그룹(FIG-A, FIG-B, FIG-C)간의 장내세균의 군집을 비교한 결과 BIG에서는 Actinobacteria 문이 전체 군집의 $74.22{\pm}3.48%$로 우점을 차지하였다. 분유그룹의 Actinobacteria 문을 비교하였을 때 FIG-A는 $73.46{\pm}4.12%$였지만 FIG-B와 FIG-C는 $66.52{\pm}5.80%$와 $68.88{\pm}4.33%$로 BIG와 FIG-A에 비해 낮은 비율을 보였다. 속(genus) 수준에서 살펴보면 Bifidobacterium이 전체 미생물군집에서 가장 높은 비율로 분포하고 있었으며 BIG가 $73.09{\pm}2.31%$로 가장 높았고 FIG-A, FIG-B 및 FIG-C는 각각 $72.25{\pm}4.93%$, $63.81{\pm}6.05%$ 및 $67.42{\pm}5.36%$를 차지하였다. Bifidobacterium 종의 경우 모든 그룹에서 Bifidobacterium longum이 우점을 차지하고 있었으며 BIG와 FIG-A가 전체 군집의 $68.77{\pm}6.07%$와 $66.85{\pm}5.80%$를 차지하였다. 이에 반해 FIG-B와 FIG-C는 각각 $58.94{\pm}6.20%$와 $61.86{\pm}5.31%$로 BIG와 FIG-A보다 낮은 비율로 분포하는 것을 분석하였다. FIG-A가 섭취한 분유는 Bifidobacterium의 선택적 증식이 우수하다고 알려진 갈락토올리고당, 갈락토실락토스, 시너지올리고당, 비피도올리고 및 장내균총개선소재를 혼합한 5-Bifidus factor 복합물이 포함되어 있다. 이와 같은 5-Bifidus factor라는 유산균에 대한 증식능이 우수한 프리바이오틱스를 혼합하였기 때문에 모유를 섭취한 영아의 장내에 존재하는 Bifidobacterium 속의 군집과 유사하게 나타난 것으로 판단된다. The intestinal microbiomes vary according to the factors such environment, age and diet. The purpose of this study was to compare the gut microbial diversity between Korean infants receiving breast-fed milk and formula-fed milk. We analyzed microbial communities in stool samples collected from 80 Korean infants using next generation sequencing. Phylum level analysis revealed that microbial communities in both breast-fed infants group (BIG) was dominated by Actinobacteria ($74.22{\pm}3.48%$). Interestingly, the phylum Actinobacteria was dominant in formula-fed infants group A (FIG-A) at $73.46{\pm}4.12%$, but the proportions of phylum Actinobacteria were lower in formulafed infants group B and C (FIG-B and FIG-C) at $66.52{\pm}5.80%$ and $68.88{\pm}4.33%$. The most abundant genus in the BIG, FIG-A, FIG-B, and FIG-C was Bifidobacterium, comprising $73.09{\pm}2.31%$, $72.25{\pm}4.93%$, $63.81{\pm}6.05%$, and $67.42{\pm}5.36%$ of the total bacteria. Furthermore, the dominant bifidobacterial species detected in BIG and FIG-A was Bifidobacterium longum at $68.77{\pm}6.07%$ and $66.85{\pm}4.99%$ of the total bacteria. In contrast, the proportions of B. longum of FIG-B and FIG-C were $58.94{\pm}6.20%$ and $61.86{\pm}5.31%$ of the total bacteria. FIG-A showed a community similar to BIG, which may be due to the inclusion of galactooligosaccharide, galactosyllactose, synergy-oligosaccharide, bifidooligo and improvement material of gut microbiota contained in formula-milk. We conclude that 5-Bifidus factor contained in milk powder promotes the growth of Bifidobacterium genus in the intestines.

동해 시 아파트 공급의 시대적 변천에 관한 연구

김경순,Kim, Kyung-Soon 한국주거학회 2006 한국주거학회 논문집 Vol.17 No.5

The purpose of this study was to explore the periodic changes of apartment supply between 1980 and 2001 in the City of Donghae. A survey was conducted with 82 apartment complexes that were built in during 21years. The data were itemized and specified by the unit house area, story, periodic and local apartment distribution. They were classified by five:(5) periods and analyzed by each apartment complexes for building characteristics. There were found that apartment housing were influenced by many primary factors such as demographics and a lot of the housing politics etc. The results of this study were as follows: 1) Apartment supply in the City of Donghae mass-produced from 1980 to 2001; Especially the forth period was supplied 5,999 households. 2) Between south area and north area was found a regional disparity. 3) The exclusive use $34{\sim}66m^{2}$ were continuously and centrally supplied according to the housing policy and practical demand. 4) For the types of story were variously found ; Especially the below 5 story apartment was continuously supplied.

Studies on the Ornithine Aminotransferase from Chicken Liver

김경순,박종옥,박광숙,Kim, Gyung-Soon,Park, Jong-Ok,Park, Kwang-Sook 생화학분자생물학회 1985 한국생화학회지 Vol.18 No.2

닭 간에서 ornitine aminotransferase는 주로 mitochondrial fraction에 존재하였다. 이 효소를 열처리, ammonium sulfate fractionation, DEAE-cellulose ion exchange chromatography, Sephadex G-200 filtration, CM-Sephadex column chromatography를 이용하여 정제해서 disc polyacrylamide gel electrophoresis를 하였더니 하나의 단백질 띠가 얻어졌다. 이 정제된 효소를 이용해서 몇가지 성질을 조사해본 결과는 다음과 갈다. SDS-polyacrylamide gel electrophoresis에 의해 측정된 분자량은 62,000이었고 gel filtration에 의한 값은 63,000이었으므로 이 효소는 monomer로 존재한다고 생각되었다. L-ornithine과 $\alpha$-ketoglutarate에 대한 $K_m$ 값은 각각 6.31 mM, 1, 03 mM 이였으며, 이 효소는 기질의 농도가 높을 때 저해되었고 또한 3 mM p-chloromercuriphenylsulfonic acid를 가해주면 80% 저해되었다. glyoxylate, oxaloacetate, pyruvate도 L-ornithine보다는 못하지만 amino group acceptor로서 작용하였으며, 효소활성에 대한 최적 pH는 7.3으로 측정되었다. Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The $K_m$ values for ornithine and $\alpha$-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than $\alpha$-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

Adsorption Behaviors of Indomethacin on Cholestyramine Resin

김경순,Kim, Kyung-Soon 한국약제학회 1988 Journal of Pharmaceutical Investigation Vol.18 No.3

In vitro studies were performed on the interaction of indomethacin with cholestyramine, a hypocholesterolemic substance. Cholestyramine showed a marked affinity for indomethacin among tested acidic drugs and the intensity of adsorption was dependent on pH, temperature and sodium chloride. Moreover, the combination of indomethacin with some acidic drugs that formed complexes with cholestyramine, considerably inhibited the adsorption of indomethacin on the resin.

닭 간의 Ornithine Aminotransferase 에 관한 연구

김경순,박종옥,박광숙 ( Gyung Soon Kim,Jong Ok Park,Kwang Sook Park ) 생화학분자생물학회 1985 BMB Reports Vol.18 No.2

Ornithine aminotransferase was purified from chicken liver and several properties of the enzyme were studied. The purified enzyme appeared to be homogeneous on polyacrylamide gel electrophoresis with and without SDS. The molecular weight of the enzyme was estimated as 62,000 by SDS-polyacrylamide gel electrophoresis, and as 63,000 by gel filtration. The K_m values for ornithine and α-ketoglutarate were found to be 6.31 mM and 1.03 mM, respectively. The enzyme was inhibited either by high substrate concentrations or by p-chloromercuriphenylsulfonic acid. Glyoxylate, oxaloacetate and pyruvate could act as amino group acceptors, but they were much less effective than α-ketoglutarate. Optimum pH of this enzyme was found to be 7.3.

난소암 치료에 대한 한의 임상 가이드라인

김경순,최홍식,김승모,유화승,Kim, Kyung-Soon,Choi, Hong-Sik,Kim, Seung-Mo,Yoo, Hwa-Seung 대한암한의학회 2016 大韓癌韓醫學會誌 Vol.21 No.2

Ovarian cancer is the tenth most common cancer in Korean female, and the third most common cancer of female reproductive organ after breast cancer and uterine cervical cancer. In spite of develop of conventional treatment, high modality of ovarian cancer comes from difficulty of an early diagnosis. Recent studies revealed that combining conventional and integrative medical treatment can reduce the adverse effect of surgical operation, chemotherapy and radiotherapy. Also it can improve survival rate, quality of life. However there isn't still a systemic clinical protocol for ovarian cancer in Korean medicine. This study will be helpful to establish clinical practice guidelines of Korean Medicine for ovarian cancer. And further studies on integrative ovarian cancer treatment are needed to build the clinical practice guidelines of ovarian cancer.

Amplification and Expression of Several Cellular Proto-Oncogenes in Squamous Cell Carcinoma Cell Lines

김경순,박노희,Kim, Kyung-Soon,Park, No-Hee 생화학분자생물학회 1990 한국생화학회지 Vol.23 No.2

사람의 정상적 epidermal keratinocyte(NHEK)와 사람의 squamous cell carcinoma cell line에서 여러 가지 cellular proto-oncogene의 존재, restriction profile, 그리고 expession을 조사하였다. NHEK와 cell line들로부터 얻은 발암유전자의 Eco RI restriction profile은 유사하였고 정상세포와 암세포들은 모두 몇 가지 proto-oncogene들을 발현하였으나 NHEK에 비해 암세포에서 이들 proto-oncogene의 발현되는 양이 많았다. 이 결과로부터 squamous cell carcinoma의 malignant transformation과 그 유지에 있어서 몇 가지 cellular proto-oncogene들의 과발현이 필요하다는 것을 알 수 있다. The restriction profiles and expression of various cellular proto-oncogenes in normal human epidermal keratinocytes and human squamous cell carcinoma cell lines were studied. Both normal human epidermal keratinocytes and cancer cell lines contained and expressed various proto-oncogenes such as erb-B-1, abl, fos, raf, H-ras, and myc. Although the Eco RI restriction profiles of the oncogenes from the normal cells and cancer cells were similar, the amount of the oncogene in the cancer cell lines was generally higher than that in the NHEK. Furthermore, the amount of expression of these proto-oncogenes was significantly higher in the cancer cell lines than in the normal keratinocytes. These data indicate that the overexpression of several cellular proto-oncogenes might be necessary for the malignant transformation and maintenance of the malignancies of squamous cell carcinoma.

상황맥락적인 피드백을 활용한 CAI가 화학 학습에 미치는 효과

김경순,정경진,차정호,강이영,노태희,Kim, Kyung-Sun,Chung, Kyoung-Jin,Cha, Jeong-Ho,Kang, Yi-Young,Noh, Tae-Hee 대한화학회 2007 대한화학회지 Vol.51 No.2

이 연구에서는 컴퓨터 보조 수업에서 상황맥락적인 피드백의 활용이 학생들의 개념 이해도, 과학 학습동기, 컴퓨터 보조 수업에 대한 인식에 미치는 효과를 조사하였다. 인천시의 한 남녀 공학 중학교에 있는 1학년 114명의 학생들을 상황맥락적 피드백 CAI, 피드백 CAI, CAI 집단에 배치하고, 8차시 동안 ‘물질의 세 가지 상태'와 ‘분자의 운동'에 대하여 수업을 실시하였다. 구획변인은 중간고사 과학 성적을 사용하였다. 이원 공변량 분석 결과, 사전 과학 성취 수준에 관계없이 상황맥락적 피드백 CAI 집단의 개념 이해도 점수가 다른 CAI 집단에 비해 높았고, 그 점수 차이가 통계적으로 유의미하였다. 상위 학생들의 경우 피드백 CAI 집단의 과학학습 동기 점수가 CAI 집단의 점수보다 통계적으로 유의미하게 높았으나, 하위 학생들의 경우에는 세 집단 간 점수 차이가 통계적으로 유의미하지 않았다. 컴퓨터 보조 수업에 대한 인식의 경우, 상황맥락적 피드백 CAI 집단과 피드백 CAI 집단의 상위 학생들은 CAI 집단의 상위학생에 비해 컴퓨터 보조 수업에 대해 긍정적으로 인식하였다. 피드백 CAI 집단의 하위 학생들은 다른 두 CAI 집단보다 부정적인 인식이 약간 높았다. 교육학적 함의를 논의하였다. This study investigated the effects of situational context feedback in CAI upon students' conceptual understanding, science learning motivation, and the perception of CAI. Seventh graders (N = 114) from a coed middle school in Incheon were assigned to the situational context feedback CAI (SCF-CAI), the feedback CAI (F-CAI) and the CAI groups, and were taught about ‘three states of matter' and ‘motion of molecules' for 8 class hours. Prior science achievement test score used as a blocking variable. Two-way ANCOVA results revealed that the conceptual understanding test scores of the SCF-CAI group were significantly higher than those of the other groups. For the higher achievers, the scores of the F-CAI group in science learning motivation test were significantly higher than those of the CAI group. However, there was no significant difference among the lower achievers of three groups. The higher achievers in the SCFCAI and the F-CAI groups perceived the CAI more positively than those of the CAI group. The lower achievers in the FCAI group perceived the CAI more negatively than those of the other groups. Educational implications are discussed.