실험적 허혈성 급성 신부전 모델에 대한 칼슘 길항제의 효과

권영주(Young Joo Kwon),이규백(Kyu Back Lee),권현민(Hyun Min Kyeon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),노정우(Chung Woo Noh) 대한내과학회 1989 대한내과학회지 Vol.37 No.4

N/A Recently, much attention has been focused on the role of calcium in mediating or propagating ischemic cell injury. Modifying alterations in cell calcium redistribution or cellular calcium influx with a variety of agents have been beneficial in ameliorating the degree of cell injury in a number of experimental settings. But studies on animal models about ischemic acute renal failure (ARF) produced either by infusion of vasoconstrictors or by interrupting renal artery blood flow have provided conflicting results. The purpose of this study was to evaluate the effect of calcium entry blockers in an ischemic ARF model by the renal artery clamp in rats. Nine cats were anesthetized with pentobarbital sodium (40mg/kg, I.M,) and a tracheostomy, an IV line and a urinary catheter were placed in position. Temperature was maintained at 37.5 degrees C. By an abdominal approach, both renal arteries were isolated. Four cats were used as controls and received saline for 2 hours before bilateral renal artery clamping. Another five cats were treated with verapamil (5㎍/kg/min.) systemically for 2 hours before bilateral renal artery clamping. All nine cats underwent 1 hour of renal artery clamp followed by 3 hours of reperfusion and hydration with saline. Before clamping and after reperfusion, blood and urine were sampled for creatinine, Na and K, and urine volume was measured. The results were as follows: In the control group, the serum creatinine level was 1.28㎍0.33㎎/dl before clamping and increased to 1.63±0.73㎎/dl after clamping; in the experimental group, the serum creatinine level was 1.20±0.33㎎/dl before clamping and increased to 1.75±0.50㎎/dl after clamping. In the control group, the Ccr value was 13.08±9.25ml/min before clamping and decreased to 0.41±0.22ml/min after clamping; in the experimental group, the Ccr value was 6.24±6.18ml/min before clamping and decreased to 0.14±0.09ml/min after clamping. But these results were not significant statistically. Our results suggested that systemic pretreatment with a calcium entry blocker (verapamil) may be ineffective in an ischemic ARF model by renal artery clamping in cats.

최근의 중학교 공간구조의 유형별 분류와 특성 고찰

권영주(Kwon.Young-Joo),김용승(Kim.Yong-Seung) 대한건축학회 2004 대한건축학회 학술발표대회 논문집 - 계획계/구조계 Vol.24 No.1

The 7th educational program based on "demander focus, learner focus, and opened education" requires many changes of school facilities.<br/> For the purpose of analyzing the characteristics of spatial structure of middle school corresponding to the 7th educational program, 25 numbers of middle schools founded during 2002~2003 in Gyeonggi-do were analyzed as the subject.<br/> At first, in order to understand the characteristics of spatial structure, 3 types of patterns such as "curriculum classroom, curriculum laboratory, home base" were categorized and it results in more three types of patterns such as "curriculum block, home base breakaway, curriculum laboratory breakaway", and after more objective analysis based on space syntax, the most opened space structure turns out to be "curriculum block type", and the most closed space structure turns out to be "home base breakaway type".<br/> <br/> <br/> <br/> <br/> <br/>

승모판 대치이식수술에 사용한 조직판막의 초음파심음향도 소견

김권배 ( Kwon Bae Kim ),김교성 ( Kyo Sung Kim ),김윤년 ( Yoon Nyun Kim ),허정욱 ( Jung Wook Hur ),김두환 ( Doo Hwan Kim ),권영주 ( Yong Joo Kwon ) 대한내과학회 1984 대한내과학회지 Vol.27 No.1

버어리주맥과 엽설의 니코틴과 nitrate함량에 의한 등급별 군집분석

한영림,성용주,권영주,김삼곤,이경구,김근수,이문수,Han, Young-Rim,Sung, Yong-Joo,Kwon, Young-Ju,Kim, Sam-Kon,Lee, Kyeong-Ku,Kim, Kun-Soo,Rhee, Moon-Soo 한국연초학회 2006 한국연초학회지 Vol.28 No.2

The grades of burley tobacco stems and scraps were classified followed by the nicotine and nitrate contents by using the cluster analysis. The chemical components of the burley tobacco stems and scraps gathered from 2002 to 2005 were analyzed with auto analyzer. According to the nicotine contents and the nitrate contents, the burley stems and scraps could be classified three groups, respectively. In case of the burley scraps, the AB3T, AB4TR, B1T and B2T grades belonged to the $1^{st}$ a group. The C1W and C2W grades belonged to the $2^{nd}$ group and the CD3W and CD4TR belonged to the $3^{rd}$. In case of the burley stems, the AB3T and AB4TR grades belonged to the $1^{st}$ group. The B1T, B2T, C1W and C2W grades belonged to the $2^{nd}$ group and the CD3W and CD4TR belonged to the $3^{rd}$ group. This classification of raw materials depending on the similarity in the chemical components might be helpful to control the properties of the Reconstituted Tobacco sheet.

신 증후 출혈열에서 신장 조직 검사의 임상적 의의

조원용(Won Yong Cho),이은주(Eun Joo Lee),김용섭(Yong Sup Kim),권현민(Hyun Min Kim),권영주(Young Joo Kwon),김형규(Hyung Kyu Kim),원남희(Nam Hee Won) 대한내과학회 1991 대한내과학회지 Vol.40 No.5

N/A To evaluate the relationship between derangement of renal function and the pathological findings in hemorrhagic fever with renal syndrome (HFRS), renal biopsy and functional studies were performed in 23 cases of HFRS. To correlate the pathologic findings with renal funtion, serum creatinine, creatinine clearance, and proteinurea were measured, and also an immunohisto-chemical study in kidney tissue was performed. The results were as follows: 1) The study population included 9 oliguric HFRS cases and 14 nonoliguric HFRS cases. Clinical manifestations at admission were more severe in oliguric cases. 2) Serum creatinine in oliguric cases at admission were 12.5±4.5 mg/dl, higher than the nonoliguric cases, 8.4±3.9 mg/dl (p<0.05). 3) There were no significant differences in the pathologic findings between oliguric and nonliguric HFRS. Pathologic findings were focal infiltration of inflammatory cells and edema of interstitium and destructive changes of tubular structure. 4) The infiltrating inflammatory cells were mostly T-lymphocytes. From the above findings, we can conclude that renal functional changes in HFRS are not related with the severity of the pathologic lesion. It is also sugsseted that infiltrating T-lymphocytes in the interstitium may be related with renal functional derangement ant the pathogenesis of proteinuria.

한국형 출혈열에서 단백뇨의 원인으로써 Cytokine 의 의의

이은주(Eun Joo Lee),이영호(Young Ho Lee),김선숙(Seon Sook Kim),차대룡(Dae Ryong Cha),권영주(Young Ju Kwon),김형규(Hyoung Kyu Kim),조원용(Won Yong Cho) 대한내과학회 1994 대한내과학회지 Vol.47 No.4

N/A Background: Recently it was proposed that T-lymphocyte infiltrated in renal interstitium might be associated with abnormal renal function and proteinuria in the hemorrhagic fever with renal syndrome (HFRS). Therefore to speculate the role of cytokines including interleukin 2 (IL-2), interferon α (IFN α) and interferon γ(IFN γ) for proteinuria and disturbed renal function in HFRS, author performed IL-2 assay in serum and urine, IFN α and IFN γ assay in urine in oliguric and diuretic phase of patients with HFRS who were proved serologically and showed typical clinical manifestations, Methods: The study population included 14 patients who had classical manifestations of HFRS with acute renal failure during clinical course. With the conventional biochemical and renal functional study, serum and urine IL-2, urine IFN α and IFN γ were measured in oliguric and diuretic phase of HFRS with radioimmunometric assay. Above measurement were also performed in control groups, who showed no renal dysfunction. Results: The results were as follows, 1) 24 hour proteinuria in oliguric phase (731.6±106.9 mg/day) was significantly higher than that in diuretic phase (90.9±89.8mg/day) (p=0.001). 2) There was no significant difference in serum concentration of Il-2 between the oliguric and diuretic phase, but there were significant difference compared with that of control group, in oliguric and diuretic phase, respectively (p<0.05). 3) The excretion of urine IL-2 of HFRS patients in oliguric phase (5.07±1.06fmol/mgCr) were significantly higher than that of the patients in diuretic phase (0.35±0.05fmol/mgCr) and control group (0.78±0.27fmol/mgCr) (p < 0.05). 4) The excretion of urine IFN g of HFRS patients in oliguric phase (5.27±4.27U/mgCr) were higher than that of patients in diuretic phase (0.27±1.12U/mgCr). Urinary excretion of IFN γ in oliguric phase (2,25±1.44U/mgCr) were significantly higher than that in diuretic phase (0.12±1.12U/mgCr) (p<0.05). 5) There was positive corre)ation between serum IL-2 and 24 hour proteinuria (r=0.67, p=0.018). Urine IL-2 showed reverse correlation with creatinine clearance (r =-0.69, p=0.013) and positive correlation with the amount of 24 hour proteinuria (r=0.80, p=0.003). 6) Urine IFN α and IFN γ showed reverse correlation with creatinine clearance (r=-0.61, p=0.03 and r =-0.67, p=0.017, respectively) and positive correlation with the amount of 24 hour proteinuria (r=0.80, p=0.003 and r=0.94, p=0.001, respectively). Conclusion: With above results it may be concluded that cytokine including IL-2, IFN α and IFN γ may have a significant role for proteinuria and renal dysfunction in the patient with HFRS.

가압경수로 고준위페기물 처분용기에 대한 크립해석

하준용,최종원,권영주,Ha Joon-Yong,Choi Jong-Won,Kwon Young-Joo 한국전산구조공학회 2004 한국전산구조공학회논문집 Vol.17 No.4

본 논문에서는 깊은 지하 500m에 처분된 가압경수로(PWR) 고준위폐기물 처분용기에 지하수압과 벤토나이트 팽윤압이 가해지는 동안 처분용기에 발생하는 크립변형을 예측하기 위하여 처분용기에 대한 구조해석을 수행하였다. 보통 이러한 크립변형은 처분용기에 추가적인 외력이 작용하지 않더라도 처분용기에 작용하는 압력과 내부의 높은 열에 의하여 발생될 수 있다. 처분용 기내부의 열분포의 복잡성 덴 시간의존성으로 인하여 일단 외부 지하수압 및 팽윤압만 고려하여 크립해석을 수행하였다. 이를 위하여 적당한 크립함수를 사용하였으며, 해석은 1억$(10^8)$초 동안 수행하였다. 해석결과 1억초 동안 발생하는 크립 변형률은 매우 작으며 주희 처분용기의 위아래 덮개에 발생함을 알 수 있었다. 그러나 처분용기의 구조강도에 중요한 내부 주철삽입물에는 훨씬 더 작은 미소한 변형률만 발생하여 처분용기에 발생하는 크립변형은 처분용기의 구조적인 안전성에는 큰 영향을 미치지 않음을 알 수 있었다. 해석 초기에 처분용기 내에 급격히 응력이 증가하여 최고치에 도달한 후 잠깐동안 이 응력 값을 유지하다가 그 이 후에는 급격히 응력 값이 감소하는 응력이완현상을 보이고 있기 때문에 발생 응력 측면에서도 전혀 처분용기의 구조적인 안전성에 문제가 없음이 확인되었다. In this paper, a structural analysis for the pressurized water reactor(PWR) spent nuclear fuel disposal canister which is deposited under the 500m deep underground is carried out to predict the creep deformation of the canister while the underground water and swelling bentonite pressure are applied on the canister. Usually the creep deformation may be caused due to the Pressure and the high heat applied to the canister even though additional external loads are not applied to the canister. These creep deformations depend on the time. In this paper, oかy the underground water and bentonite swelling Pressure are considered for the creep deformation analysis of the canister, because the heat distribution inside canister due the spent fuel is not simple and depends on time. A proper creep function is adopted for the creep analysis. The creep analysis is carried out during $10^8$ seconds. The creep analysis results show that the creep strains are very small and these strains occur usually in the lid and bottom of the canister not in the cast iron insert. A much smaller strain is found in the cast iron insert. Hence, the creep deformation doesn't affect the structural safety of the canister, and also the creep stress which shows the stress relaxation phenomenon doesn't affect the structural safety of the canister.

신장이식 환자에서 수술후 2 개월 이내의 핍뇨분석

권오상(Oh Sang Kwon),권영주(Young Joo Kwon),이영기(Young Gee Lee),정길만(Gil Mann Jung),김난희(Nan Hee Kim),장미경(Mi Kyoung Jang),김용섭(Yong Sub Kim),구자룡(Ja Ryong Ku),차대룡(Dae Ryong Cha),조원용(Won Yong Cho),표희정(Heui Jung P 대한내과학회 1998 대한내과학회지 Vol.54 No.1

N/A Objective: Graft survival rate has been improved due to newly developed immunosuppressive agents, care of recipient and operative method. However, since many risk factors are still threatening the graft survival, many studies have been underway to identify such factors, one of which has been on delayed graft function(DGF). Extending the definition of DGF to oliguria within 2 months postoperative period(POP), we began this study in order to evaluate what effects oliguria within 2 months POP have on graft survival and what are the risk factors involved. Methods: 103 patients who have had renal transplantation performed were divided into two groups (oliguric group and non-oliguric group), based on the presence or absence of oliguria within 2 months POP. Risk factors such as the recipient factors(age, gender), donor factors(age, gender), operative factors (warm ischemia time, intraoperative urine volume), HLA typing, postoperative hypotension, postoperative hypovolemia were compared between the two groups and the impact of oliguria on graft outcome was also analysed. Results: 1) 14 were Oliguric patients and 89 were non-oliguric patients. 2) One-year graft survival rate was 40% in the oliguric group and 98% in the non-oliguric group (P<0.05). 3) As the result of analyzing the risk factors, non living related donor(living non-related donor and cadaver donor) were 7(50%) in the oliguric group and 16(18%) in the non-oliguric group(P<0.05). The mean intraoperative urine volume was 442ml in the oliguric group and 774ml in the non-oliguric group (P<0.05). The occurrence of postoperative hypotension were 5(36%) in the oliguric group and 1 (1%) in the non-oliguric group(P<0.05). Other risk factors such as the recipient factors, donor factors, warm ischemia time, HLA typing and postoperative hypovolemia were not significantly different between the two groups. Conclusion: Graft survival rate in the oliguric group was lower than in the non-oliguric group. The risk factors for oliguria were non living related donor, intraoperative urine volume lower than 500ml and postoperative hypotension. In conclusion, renal transplantation from non living related donor needs to be proceeded with caution; the maintenance of intraoperative urine volume and the prevention of postoperative hypotension are essential for better graft outcome.

ESI-MS/MS를 이용한 소변 중 Globotriaosylceramide(Gb3)의 정량 및 임상 응용; 패브리병(Fabry) 진단

윤혜란,조경희,강승우,권영주,정춘식,이용수,Yoon, Hye-Ran,Cho, Kyung-Hee,Kang, Seung-Woo,Kwon, Young-Joo,Jeong, Choon-Sik,Lee, Yong-Soo 대한약학회 2007 약학회지 Vol.51 No.2

Measurement of globotriaosylceramide (Gb3, ceramide trihexoside) in urine has clinical importance for monitoring after enzyme replacement therapy in Fabry disease patients. The disease is an X-linked lipid storage disorder that results from a deficiency of the enzyme ${\alpha}$-galactosidase A (${\alpha}$-Gal A). The lack of ${\alpha}$-Gal A causes an intracellular accumulation of glycosphingolipids, mainly Gb3. A simple, rapid, and highly sensitive analytical method for Gb3 in urine was developed without labor-extensive pre-treatment by electrospray ionization MS/MS (ESI-MS/MS). Only simple 5-fold dilution of urine is necessary for the extraction and isolation of Gb3 in urine. Gb3 in diluted urine was dissolved in dioxane containing C17:0 Gb3 as an internal standard. After centrifugation it was directly injected and analyzed through guard column by in combination with multiple reaction monitoring mode of ESI-MS/MS. Eight isoforms of Gb3 were completely resolved from urine matrix. C24:0 Gb3 occupied 50% of total Gb3 as a major component in urine. Linear relationship for Gb3 isoforms was found in the range of 0.005${\sim}$5.0 ${\mu}$g/ml. The limit of detection (S/N=5) was 0.005 ${\mu}$g/ml and limit of quantification was 0.05 ${\mu}$g/ml for C24:0 Gb3 with acceptable precision and accuracy. Correlation coefficient of calibration curves for 8 Gb3 isoforms ranged from 0.9598 to 0.9975. This method could be useful for rapid and sensitive 1st line Fabry disease screening, monitoring and/or diagnostic tool for Fabry disease.

지속적 외래 복막 투석이 당뇨성 신부전 환자의 β2-Microglobulin 에 미치는 영향

최태승(Tae Seung Choe),김선숙(Sun Sook Kim),권영주(Young Joo Kwon),조원용(Won Yong Cho),김형규(Hyoung Kyu Kim),원동준(Dong Joon Woo) 대한내과학회 1991 대한내과학회지 Vol.41 No.3

N/A To evaluate the effect of CAPD on the removal of beta-2-microglobulin (B2MG) in the patients with diabetic renal failure, serum levels and clearances of creatinine, urea, and B2MG, ultrafiltration rates, mass transfer raters of B2MG, and equilibration curves were determined in 5 diabetics and 5 nondiabetics with chronic renal failure undergoing CAPD. The study was carried out using peritoneal dialysis solutions with 1.5% dextrose for 240minutes of dwell time. The valuse of plasma were the means of 2samples taken at the start and after 240minutes. The values of peritoneal dialysis solution were determined on 3mlsamples taken at the 30minutes, 60minutes, 120minutes, and 240minutes after dialysate infusion. The authors results are as follows; 1) The serum R2MG levels were 39.4mg/1 in nondiabetic and 18.2mg/1 in diabetic renal failure groups which were significantly higher values than normal values and significantly lower values than that of hemodialyzed patients. 2) The peritoneal clearances of B2MG were 0.64 ml/ min in nondiabetic and 0.90ml/min in diabetic renal failure groups which represented 7.7% and 11.0% of dialysate flow rate in each groups. In diabetic renal failure group, patient 'age and mass transfer rate of B2MG were positively correlated with the peritoneal clearance of BRMG (r=0.25 and 0.76). 3) The equilibration curves of creatinine and urea were well fitted to their standard equilibration curves in both groups. In diabetic renal failure group, the dialysate to plasma ratios of creatinine and urea showed the linear correlation with the in-dwelling time during the 240minutes of dwell time (r=0.9964 and 0.9742) 4) The equilibration curves of B2MG showed that the D/P ratios of the diabetic group were constantly higher than those of nondiabetic group. The equilibration slopes between 120minutes and 240minutes after indwelling were significantly lower than those between the start and 120minutes after dwelling in each nondiabetic and diabetic groups. In conclusin, the standard CAPD may allow a constant B2MG removal diabetic renal failure patients by the means of diffusion and increased peritoneal vascular permeability of B2MG.